찔레뿌리 초임계 추출물의 항균, 항산화 및 항염증 활성

고영심,최선은 한국식품저장유통학회 2018 한국식품저장유통학회지 Vol.25 No.2

The roots of Rosa multiflora Thunberg have been used in traditional oriental medicine as remedies for rheumatic arthralgia and scabies. In this study, the anti-fungal, anti-oxidant, and anti-inflammatory activities of a supercritical extract of Rosa multiflora root were investigated in vitro. To investigate the anti-oxidant and anti-inflammatory effects of the supercritical extract, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, and the inhibition of nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were examined, respectively. In addition, the anti-fungal activities of the extract were assessed. The results showed a concentration-dependent, increase in ABTS radical scavenging activity. The supercritical fluid extracts of Rosa multiflora root exhibited low toxicity to RAW 264.7 cells at 100 µg/mL the highest concentration tested. Cells stimulated with LPS produced more nitric oxide than normal control cells; however, cells treated with the supercritical fluid extract decreased this production in a concentration-dependent manner. Finally, the supercritical fluid extracts showed significant anti-fungal activity. These results suggest that extracts of the roots of Rosa multiflora might be used to develop potent anti-fungal, anti-oxidant, and anti-inflammatory agents, and may be useful as ingredients for related new functional cosmetic materials.

중소병원 간병인의 표준주의에 대한 인지도와 수행도

고영심,박보현 대한의료관련감염관리학회 2016 의료관련감염관리 Vol.21 No.2

Background: There is not enough evidence about the level of awareness and performance for healthcare-associated infection control among caregivers working in medium and small hospitals. Methods: Self-reported questionnaires were distributed to 144 caregivers who worked at nine medium and small hospitals in Gyeongnam Province. The questionnaire comprised a total of 15 items outlining standard precautions. Results: The subjects who had less than two years of work experience showed significant differences between awareness and performance in three domains: hand washing (F=4.41, P=.006), protective equipment (F=4.18, P=.008), and linen and environment (F=4.74, P=.004). There was a significant difference between awareness and performance among those working 24 hours in the following areas: hand washing (F=4.19, P=.018) and protective equipment (F= 7.31, P=.001). Conclusion: There were significant differences between awareness and performance associated with the importance of infection control, heavy workloads from long working hours, joint caretaking responsibilities, and short careers of less than two years. There is therefore a need to shorten the long working hours of caregivers.

소방공무원의 대처, 자기노출, 반추, 조직문화가 외상 후 성장에 미치는 영향

고영심,하영미,김지아,조한아 한국디지털정책학회 2020 디지털융복합연구 Vol.18 No.4

반복적 외상 사건을 겪은 소방공무원의 외상 후 성장 경험

고영심,하영미,Ko, Youngshim,Ha, Yeongmi 한국직업건강간호학회 2021 한국직업건강간호학회지 Vol.30 No.3

Purpose: The study aimed to explore experiences of the posttraumatic growth (PTG) in firefighters with repeated exposure to traumatic events. Methods: Participants were 11 firefighters from two fire departments, who had experienced more than one critical trauma events. Data were collected through personal interviews from August to October 2020 and analyzed by Colaizzi's phenomenological methods. Results: The PTG experiences were derived into four categories: 'growth in self-perception', 'rediscovery of the meaning of life', 'deep interpersonal relationships', and 'discovery of the meaning of work'. Conclusion: These findings could be used as basic information for developing PTG program for firefighters such as logo-therapy, semantic therapy, and self-disclosure intervention using expressive writing and speaking.

온라인 수업의 질, 셀프리더십이 간호대학생의 학업적 자기효능감에 미치는 영향

고영심,한상미,Ko, Youngshim,Han, Sang Mi 한국융합학회 2021 한국융합학회논문지 Vol.12 No.4

본 연구는 온라인 수업을 경험한 간호대학생들의 온라인 수업의 질, 셀프리더십 및 학업적 자기효능감의 정도를 파악하고 학업적 자기효능감의 영향요인을 규명하고자 실시하였다. 연구대상은 일 대학의 간호대학생 115명이었으며, 연구설계는 구조화된 설문지를 통해 자료를 수집한 서술적 조사연구이다. 연구결과 간호대학생의 학업적 자기효능감에 셀프리더십(β=.431, p=<.001)과 온라인 수업의 질 하위요인인 수업환경(β=.314, p=.004)이 유의한 영향요인으로 나타났다. 이 변수들은 간호대학생의 학업적 자기효능감을 총 37.7% 설명하는 것으로 나타났다. 본 연구결과를 바탕으로 온라인 수업을 받는 간호대학생의 학업적 자기효능감을 향상을 위해 셀프리더십 함양과 온라인 수업 환경개선의 기초자료로 활용되기를 기대한다. This study aimed to examine the online lecture quality, self-leadership, and degree of academic self-efficacy of nursing students who experienced online lecture and to identify the factors that influence academic self-efficacy. The subject of this study was 115 nursing students at one university, and the study design is a descriptive survey study that collected data through a structured questionnaire. As a result of this study, self-leadership(β =.431, p=<.001) and online class environment(β =.314, p=.004), a sub-factor of online lecture quality, were significant influencing factors on the academic self-efficacy of nursing college students. These variables explained 37.7% of the total variance in academic self-efficacy. Based on the results of this study, it is expected that it will be used as basic data for cultivating self-leadership and improving the online class environment in order to improve the academic self-efficacy of nursing students taking online classes.

자기계발동기유형과 직무수행변인간 차별적 영향관계와 심리적 안녕감의 조절효과

고영심(Ko Young Sim),강영순(Kang Young Soon) 제주대학교 관광과경영경제연구소 2014 産經論集 Vol.33 No.-

개인-환경적합성, 다중초점 몰입, 다중초점 조직시민행동간 차별적 영향관계와 몰입유형의 매개효과

고영심(Ko Young Sim),강영순(Kang Young Soon) 한국인사관리학회 2015 조직과 인사관리연구 Vol.39 No.2

본 연구는 대상유사성모형을 적용하여 연구대상 변인들을 조직, 직무, 사람의 세 가지 초점으로 분류한 후, 다음과 같은 목적을 달성하기 위해 수행되었다. 첫째, 개인-환경적합성(POF, PJF, PSF)이 다중초점 몰입유형(조직몰입, 직무몰입, 상사몰입), 다중초점 몰입유형이 다중초점 조직시민행동(OCBO, OCBJ, OCBS)에 미치는 차별적 영향력을 규명하였다. 둘째, 개인-환경적합성(POF, PJF, PSF)과 조직시민행동 유형(OCBO, OCBJ, OCBS)들 사이에 작용하는 몰입유형(조직몰입, 직무몰입, 상사몰입)의 매개효과를 규명하였다. 연구수행을 위한 자료수집방법은 동일방법편의를 최소화하기 위해 측정시기를 분리하는 방법을 활용하였으며, 연구표본은 서울과 제주지역의 공사기업 및 공무원들을 대상으로 하였다. 분석결과를 요약하면 다음과 같다. 첫째, 조직몰입에는 POF, PJF, PSF 순으로, 직무몰입에는 PJF, POF, PSF 순으로, 상사몰입에는 PSF, POF, PJF 순으로 각각 차별적 영향을 미치고 있다. 둘째, OCBO에는 조직, 직무, 상사몰입 순으로, OCBJ에는 직무, 조직, 상사몰입 순으로, OCBS에는 상사, 직무, 조직몰입 순으로 차별적 영향을 미치고 있다. 셋째, 조직몰입과 직무몰입은 개인-환경합성유형(POF, PJF, PSF)과 조직시민행동유형(OCBO, OCBJ, OCBS)의 관계를 부분매개하고, 상사몰입은 개인-환경적합성유형(POF, PJF, PSF)과 OCBO, OCBS의 관계를 부분매개하고 있다. 연구결과를 토대로 시사점을 제시하면 다음과 같다. 첫째, 연구변인들을 다중초점으로 분류한 후, 대상유사성모형을 통해 차별적 관계를 사후검증을 통해 실증적으로 규명한 초기연구이다. 둘째, OCBJ의 개념화를 시도한 후, 이를 실증적으로 연구하여 OCB의 연구범주 확대에 기여하고 있다. 셋째, 개인-환경적합성과 결과변인 사이에 작용하는 매개변인을 규명하여 관련연구의 활성화에 도움을 주고 있다. The present study conducted in the perspective of target similarity model after classified foci of research target toward organization, job, and person. The purpose of current research is as follows. First, differential effects of person-environment fit(POF, PJF, PSF) on multifoci types of commitment(organizational commitment; OC, job commitment: JC, superior commitment: SC) and multifoci types of commitment on OCB(OCBO, OCBJ, OCBS) were demonstrated. Second, commitment types (OC, JC, SC) as mediators in the relationships between person-environment fit (POF, PJF, PSF) and OCB(OCBO, OCBJ, OCBS) were tested. Temporal separation of measurement was utilized to minimize common method bias by self-report measurement, and data applied in the analysis was collected from employees in state-owned companies, private companies, and government officers in Seoul and Juju. The results are as follows. First, size of relative effects, 1) on OC were POF, PJF, PSF next, 2) on JC were PJF, POF, PSF and last, 3) on SC were PSF, POF, PJF. Second, size of relative effects, 1) on OCBO were OC, JC, SC next, 2) on OCBJ were JC, OC, SC and, 3) on OCBS were SC, JC, OC. Third, OC and JC partially mediated the relationship between person-environment fit(POF, PJF, PSF) and OCB(OCBO, OCBJ, OCBS), and SC partially mediated the relationships between person-environment fit(POF, PJF, PSF) and OCBO, OCBS respectively. The implications of current study are follows. First, the approach after classified multifoci toward research variables is an initial attempt to test differential relations by post-analysis through target similarity model empirically. Second, after conceptualizing OCBJ, the concept was empirically tested, and the attempt contributes to expand research area of OCB. Third, results on mediation between personenviron ment fit and outcome variables help to facilitate similarity research.

자기효능감과 자기계발동기 및 혁신행동과의관계

고영심(Young-Sim Ko),김희철(Hee-Cheol Kim) 한국무역연구원 2014 무역연구 Vol.10 No.1

The current study was designed to exannne in comprehensively both contexts. First, we examined the relationships of self-efficacy to 4 types of self-development motivation and 4 types self-development motivation to innovative behavior. Second, mediating effects of self-development motivation were examined in the relationship between self-efficacy and innovative behavior. The data which was applied to analysis collected from 242 employee s who have worked for state-owned companies and private companies in Jeju island which were well-equipped the competence development. The results were 1) self-efficacy was related with introjected regulation, identified regulation, and intrinsic regulation positively, but not with external regulation, 2) both introjected regulation and intrinsic regulation were associated with innovative behavior positively but not with external regulation and identified regulation, 3) partial mediating effect by introjected regulation and intrinsic regulation were proved in the relationship between self-efficacy and innovative behavior. Implications are as follow. First, self-determination theory which was mainly studied in psychology and pedagogy was introduced in organizational psychology by the present study. It contributes to facilitate the research of self-determination theory in organizations. Second, the results of which self-efficacy affects self-development motivation require a variety of program, which improve self-efficacy of employees, from companies in order to enhance intrinsic regulation for self-development. Third, we offer the necessity of human resource management system and learning programs so as to improve intrinsic self-development motivation and to increase innovative behavior.

Grignard 시약과 브로모벤질과의 반응에서 첨가전이 금속이온의 영향 연구

김정균,고영심,윤웅찬,김민숙,Jack C. Kim,Young-Sim Koh,Ung-Chan Yoon,Min-Sook Kim 대한화학회 1993 대한화학회지 Vol.37 No.2

Grignard 시약,CH_3$MgI와 브로모벤질과의 반응은 전자전달과정(SET)과 라디칼 이온쌍 중간체를 거쳐 생성되는 동종짝지음 생성물 bibenzyl을 주생성물로 이온성 공격에 의해 생성되는 교차짝지음 생성물, 에틸벤젠을 부생성물로 생성시키며 전이금속 불순물이 거의 제거된 순수 금속 Mg으로 CH_3$MgI을 제조하였을 때 그 비는 78:22이었다. 그러나 실험실용 금속 Mg을 사용하였을 때 그 비는 감소하여 67:33이었으며 이 비는 촉매량으로 첨가시킨 $FeCl_3$ 양의 증가로 더욱 감소되어 최고 20:80 비로 Fe 이온은 이온성 교차생성물, 에틸벤젠을 주생성물로 유도하였으며 이러한 반응결과는 좋은 수소원자주게로 디이소프로필 에테르를 디에틸 에테르 대신 용매를 사용하였을 때에도 큰 변화가 관찰되지 않았다. 이러한 관찰은 Grignard 시약과 유기할로겐 화합물이나 카르보닐 화합물과의 반응에서 촉매 양의 전이금속염이 전자전달과정 속도를 증가시켜 동종짝지음 이합체 생성물의 증가를 일으키는 보고와 반대의 경향을 보인다. 이러한 촉매량의 $FeCl_3$ 첨가가 CH_3$MgI의 이온성 공격의 증가와 그에 따른 교차짝지음 생성물의 증가로 유도하는 것은 브로모벤질과 반응 중 생성된 Fe 종과의 착화합물 형성에 의해 벤질탄소-브롬 결합이 CH_3$MgI의 탄소음이온 공격에 대해 반응성이 증가되는 것으로 설명하였다. 이러한 설명은 2-페닐-1-브로모에탄의 CH_3$MgI와의 반응연구에서 관찰된 반응성 결여가 뒷받침하고 있다. The effect of ferric ion on the reaction of CH_3$MgI with benzylbromide was investigated by determining the product ratio between cross-coupling product, ethylbenzene (A) and homocoupling product, bibenzyl (B) in the presence of ferric ion. When CH_3$MgI prepared with pure magnesium was used, the ratio of A to B was 22 to 78 and with reagent grade magnesium, the ratio became 33 to 67 indicating that metallic impurities in magnesium affect the reaction mechanism to lead less homocoupling product, B. The ratio changes became significant when ferric chloride was added in the reaction mixture in catalytic amounts and the ratio of A to B reached to 80 to 20 at maximum. The reaction in the presence of ferric ion seems to follow mainly an ionic mechanism which involves iron-benzyl bromide ${\pi}$-complex formation. The complex formation is expected to be able to enhance ionic attack of CH_3$MgI on benzyl carbon to give more A.

아미노산으로부터 3-(2-Chloroethyl) hydantoin들의 합성과 그들의 항암작용 평가

김정균,윤이규,고영심,윤웅찬,박무영,문경호 대한약학회 1983 약학회지 Vol.27 No.4

Six hydantoin derivatives, 3-(2-chloroethyl) hydantoin (6a), 3-(2-chloroethyl)-5-isopropylhydantoin (6b), 3-(2-chloroethyl)-5-isobutylhydantoin (6c), 3-(2-chloroetbyl)5-(2-butyl) hydantoin (6d), 3-(2-chloroethyl)-5-benzylhydantoin (6e), 3-(2-chloroethyl)-5-(indolyl-3-methyl) hydantoin (6f), were prepared by the treatment of the corresponding salt of amino acids with 2-chloroethyl isocyanate in cold water, followed by refluxing in concentrated HCl solution. Anticancer activity of the synthesized hydantoin derivatives were examined on murine leukemia L1210 cells growing in Fischer medium. Among them, 3-(2-chloroethy)-5-isobutyl-hydantoin (6c) showed substantially low $ED_{50}$ value of $9.6{\mu}g/ml$.