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이호식,강현욱,장경욱,권영수,김태완,이원재,Lee, Ho-Shik,Kang, Hyen-Wook,Jang, Kyung-Uk,Kwon, Young-Soo,Kim, Tae-Wan,Iwamoto, M.,Lee, Won-Jae 한국전기전자재료학회 2006 전기전자재료학회논문지 Vol.19 No.11
Monolayers of lipids on a water surface have attracted much interest as models of biological membranes, but also as precursors of multilayer systems promising many technical applications. They exhibit very interesting physico-chemical properties as two-dimensional and interfacial systems. Until now, many potential methodologies have been developed in order to gain a better understanding of the relationship between the structure and function of the monolayers.
파라쿼트 농도에 따른 HepG2 세포의 물리적 특성 변화와 실시간 모폴로지 관찰
이동윤(Dong-Yun Lee),강현욱(Hyen-Wook Kang),Hiroshi Muramatsu,권영수(Young-Soo Kwon) 대한전기학회 2009 대한전기학회 학술대회 논문집 Vol.2009 No.7
Paraquat is well-known to cause hepatotoxic responses in human and other mammal species. In solution, it forms free radicals and charge-transfer complex of which formation plays an important role in determination of its biological activity in the presence of various anions. The HepG2 cells were cultured onto a quartz crystal sensor which is possible to detect the density and a viscosity changes using the resonance frequency (F) and the resonance resistance (R). The plot of F-R diagram is able to explain the rheological change of cells onto the surface of the quartz crystal sensor. In this paper, we investigated the physical properties of the HepG2 cells cultured onto a ITO electrode of the quartz crystal sensor according to the paraquat injection at various concentrations (100 ㄹmM, 10 mM, 1 mM). We also observed the morphological changes with a micro CCD camera, simultaneously. The HepG2 cells were cultured onto the ITO electrode surface of the quartz crystal modified a collagen film in CO₂ incubator. After the paraquat injection, we observed the changes of the morphologies by the micro CCD camera depending on time and analyzed the physical changes of cells on the electrode surface of quartz crystal using F-R diagram. From all results, we proved the effect of paraquat at various concentrations which is led to an apoptosis such as weakening and death of the cells by oxidation and reduction reaction that were produced the superoxide anions and other free radicals.