다섯 가지 해조류 에탄올 추출물의 항돌연변이 활성 및 암세포 성장억제 효과

김성애(Sung-Ae Kim),김진(Jin Kim),우미경(Mee-Kyung Woo),곽충실(Chung Shil Kwak),이미숙(Mee Sook Lee) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.4

한국과 일본을 비롯한 극동아시아 지역에서 널리 사용되고 항암효과가 있는 것으로 알려진 톳, 다시마, 미역, 파래, 김을 대상으로 각 해조류 에탄올 추출물을 Ames test를 이용하여 돌연변이 유발 억제능을 검색하고, MTT assay를 이용하여 HeLa cell, MCF-7 cell과 SNU-638 cell에 대한 암세포 성장억제 효과를 비교한 결과는 다음과 같다. 5종의 해조류 에탄올 추출물의 2-anthramine에 대한 간접작용 돌연변이능 억제효과를 S. Typhimurium TA 98에서 측정한 결과, 톳(100%), 다시마(94.2%)가 3.5 ㎎/plate에서, 파래(68.5%)가 4.5 ㎎/plate에서 효과적인 돌연변이 억제능을 나타냈고, S. Typhimurium TA 100에서는 파래(100%), 톳 (100%), 다시마(94.1%)가 각각 1.5 ㎎/plate, 2.5 ㎎/plate, 4.5 ㎎/plate의 농도에서 최대 돌연변이 억제 효과를 보였다. 반면 미역과 김은 간접작용 돌연변이 억제 효과가 없는것으로 나타났다. 직접작용 항돌연변이능을 S. Typhimurium TA 98로 측정한 결과, 톳, 미역과 파래 모두 4.5 ㎎/plate의 농도에서 각각 83%, 73%, 73%의 저해율을 보여 2-nitroflouorene의 돌연변이능에 대한 저해율이 비교적 높은 것으로 나타났다. 또한 S. Typhimurium TA 100에서 sodium azide의 돌연변이능에 대한 저해효과가 가장 큰 해조류는 톳, 다시마와 파래로 세 가지 해조류 모두 3.5 ㎎/plate의 농도에서 100% 저해율을 보였다. 반면, S. Typhimurium TA 98에서 미역과 김은 4.5 ㎎/plate의 농도에서 각각 73%, 50% 정도의 저해율을 나타냈고, S. Typhimurium TA 100에서는 0% 저해율을 보여 직접작용 돌연변이에 대한 저해 능력이 적은 것으로 나타났다. MTT assay를 이용하여 HeLa cell과 MCF-7 cell, SNU-638 cell에 대한 암세포 성장억제 효과를 관찰한 결과, 5종 해조류의 에탄올 추출물 모두 암세포 증식을 억제하였다. HeLa cell의 성장을 90% 이상 저해한 해조류는 톳, 다시마, 미역, 파래였고, 그 효과를 건조무게로 비교하면 파래, 미역, 다시마, 톳의 순이었다. 김은 56% 저해율을 나타냈다. MCF-7 cell에 대한 세포 성장억제 효과 역시 90% 이상 저해율을 나타낸 해조류는 파래, 미역, 다시마, 톳의 순이었고, 김은 50%의 저해율을 나타냈다. SNU-638 cell에 대한 세포 성장억제 정도는 HeLa cell이나 MCF-7 cell에 대한 세포성장 억제 효과보다는 다소 낮았다. 저해율이 70% 이상인 해조류는 파래, 미역, 다시마, 톳이었고, 김은 68%로 나타나 해조류 간에 차이는 적었다. 이상에서 Ames test와 MTT assay를 통해 해조류 에탄올 추출물의 항돌연변이 효과와 암세포 성장억제 효과를 검색한 결과, 톳, 파래와 다시마는 항돌연변이 효과 및 암세포 성장억제 효과가 우수한 것으로 나타났고, 암세포에 대한 항암 효과는 각 시료마다 정도의 차이는 있으나 5종의 해조류 모두 암세포 증식을 억제하는 것으로 나타났다. 이 결과는 해조류가 개발 가능한 고부가가치의 기능성식품 소재임을 확인한 것으로서, 이를 위해서는 갈조류, 녹조류, 홍조류 등의 해조류에 함유된 암예방 또는 항암작용을 가진 새로운 생리활성물질을 탐색할 필요가 있다. The protective effects of ethanol extracts from 5 seaweeds on the mutagenic and cytotoxic damage were evaluated. They were separately extracted using ethanol from dried samples at room temperature, and freezedried. The inhibition effects on the mutagenicity in Salmonella assay by Ames test and cancer cell inhibitory effect in HeLa cell, MCF-7 cell and SNU-638 cell by MTT assay were assayed. Seaweed fusiforme, sea tangle and green laver showed strong inhibitory effect against 2-nitrofluorene, sodium azide- or 2-anthramine-induced mutagenicities in Salmonella Typhimurium TA 98 and TA 100 at the level of 2.5 mg ethanol extract per plate. Cancer cell inhibitory effect was shown with all of the seaweed extracts. Green laver, sea mustard, sea tangle and seaweed fusiforme showed strong cytotoxicity against HeLa and MCF-7 cells, with inhibiting by 92~93% and 89~92%, respectively. These data show that 5 seaweeds tested in this study might be potent functional foods for cancer prevention, and consumption of these seaweeds in adequate amount is recommended.

환경조화형 지속적 농업에 관한 고추 영농실태 및 선호 분석

金忠實 慶北大學校 經濟經營硏究所 1996 經商論集 Vol.24 No.2

主要 田作目의 供給反應函數에 관한 比較分析

김충실,이순석 慶北大學校 經濟經營硏究所 1994 經商論集 Vol.22 No.2-3

Farmers' management behavior has been constrained by the trade liberalization. Therefore, the purpose of this study is to inquiry farmers' intention and examine the crop system of the upland crops in Kyungpook Province in recent. The main methodology of this study is to estimate the supply response functions for major upland crops, using time-series data as well as cross-sectional data. Main results are as follows : If current prices were 100. The expectation prices of the major upland crops in open-market days. - Red pepper, seasame, bean, apple, celery cabbage, grapes and water melon would be 67.3, 59.0, 71.1, 80.4, 68.6, 75.0, 82.1 respectively. If the current land size were 100, The farmer's cultivating land of red pepper, seasame, soybean, apple, cabbage, grapes and water melon would be 68.9, 52.9, 74.7, 77.3, 85.0, 91.4, and 99.2. The price elasticities in cross-section analysis are much higher than those in time-series analysis. From the above results, the following implications can be drawn. Apple, red pepper, seasame and soybean, whose productions are expected to face a big change, are needed to establish a production system through a long-run master plan that is initiated by the local government.

식품산업의 전자상거래 이용실태 및 전망 : 선진국의 현황을 중심으로 Based on Conditions of Developed Countries

김충실,박재화 慶北大學校 經濟經營硏究所 1999 經商論集 Vol.27 No.2

GIS를 이용한 지역농업 특성 분석과 정책방향

김충실,이상호 韓國農業政策學會 1999 농업경영정책연구 Vol.26 No.1

텍스트문법에 기초한 한국어 읽기 교육 방안 -초급과 중급학습자를 대상으로-

김충실 ( Zhong Shi Jin ) 한국텍스트언어학회 2011 텍스트언어학 Vol.30 No.-

The thesis discusses the theories of reading education and the previous research on reading education of Korean in China, then it analyses the reason why the reading education research is laggard in China. The paper also focuses Formal Schema on the foundation of reading educational theories and research achievements, and it insists that it is necessary to put the semantics-grammar import method and the vocabulary-centered method. Meanwhile, it presents the point that the reading methods should change along with the change in the type of discourses.

모델링을 이용한 밀식사과원의 재식거리, 재식방향 및 수고에 따른 광 이용 효율성 비교

김충실(Chung-Sil Kim),최병호(Byeong-Ho Choi),정영주(Young-Ju Jung),윤태명(Tae-Myung Yoon) 한국원예학회 2023 원예과학기술지 Vol.41 No.3

農工地區開發事業이 農外所得增大에 미치는 效果硏究 : 古鏡, 東面, 鎭川, 墨溪 農工地區를 중심으로 한 比較分析

金忠實,李東鎬,윤경호,兪鎭采,尹秉都 경북대학교 경제경영연구소 1989 經商論集 Vol.17 No.3

·The main objectives of this study are lies on examinging indirect or direct impacts on non-farm income by rural industrialization program. ·This study made a comparative analysis on effects by 4 industrial complexes being sampled one complex in Youngnam, Honam, Chungcheong and Kangwon regions respectively among seven rural industrial complexes appointed across the country in 1984. ·Effects on non-farm income of the rural industriazation program; The major income effect by this program consist of wage income that farm house-hold can earn from rural industrial complex, though we can consider induced effects. The average annual wage income as a non-farm income from the complex is 940 million won per industrial complex, the local farm house-hold share is 77.4% of that (but in case of Shinjung complex 2,115 million won and 85.7% respectively). This average income per the complex is equivalent to farm income to be earned by 302 ha in rice production in 1987. ·In addition to this monetary (quantitative) income effect, we must consider effect on the quality of life in rural society. The average rate of substitution of the new opportunity (altering his job from farming to manufacturing) for monetary cost is 52.7% meaning that farm income is undervalued than that of non-farm activities in the complex. This rate is regarded as a kind of opportunity cost for altering job of our farmers being in agony. ·Employment effect of rural industrial complex; Employment structure, by age, of the enterprises in rural industrial complex, consist of 42% (20-29 year old) and 33.6%(30-39 year old) respectively, by educational attainment high school graduates 55.6%, middle school graduates 22.9%, and college 10%. Farm house-hold share of the total employment is 57%, and the local farm house-hold ratio of that 85%. Total employment number of the 4 industrial complexes are 1723, yet, in a word, employment effect does not reach greatly to employment goal(1 person per 2 farm house-hold). ·Financial statements analysis; According to financial structure analysis, total assets turnover of the complex is far lower than the average of medium enterprises (manufacturing) in Korea, net worth to total assets is somewhat higher than that of medium enterprises but it is not reached greatly theoretical standards. Cost of sales to net sales, though there are differences among enterprises, is 74.5%, that the ratio shows higher margin than that of the medium enterprises (manufacturing), but normal profit to net sales and net profit ratio are calculated so weak, the average break-even point ratio of the complex measured 102.4%

농업정책목표(農業政策目標)의 체계화(体系化) 1

김충실 경북대학교 농업과학기술연구소 1984 慶北大農學誌 Vol.2 No.-

Degree of difficulty in policy problems depends on a gap between present situation and desiriable situation. It is a international common sense that Agricultural policy is performed on the basis of protectionism, but yet it seems to be a hot issue in Korea, and there are many difficult problems in agricultural policies. Natural of these problems is interdependent. and a more efficient approach to these is to be systematic one. Therefore, in this paper, as a basic study to make more desirable agricultural policy, a basic structure for the systemization of goals or targets of Agricultural policy is studied. Because value judgement is much difficult matter in normative approach, basic goals of agricultural policy used in this study are selected among collective preference indicators which "are being required" or "should be required" clearly in our society (Korea), and the context of recent agricultural policies history in OCED countries is referenced to gain this goals in Agricultural policies.

農業政策目標의 體系化(Ⅰ)

金忠實 慶北大學校 1985 農業科學技術硏究所報 Vol.2 No.-

Degree of difficulty in policy problems depends on a gap between present situation and desirable situation. It is a international common sense that Agricultural policy is performed on the basis of protectionism, but yet it seems to be a hot issue in Korea, and there are many difficult problems in agricultural policies. Natural of these problems is interdependent, and a more efficient approach to these is to be systematic one. Therefore, in this paper, as a basic study to make more desirable agricultural policy, a basic structure for the systemization of goals or targets of Agricultural policy is studied. Because value judgement is much difficult matter in normative approach, basic goals of agricultural policy used in this study are selected among collective preference indicators which「are being required」or「should be required」clearly in our society(Korea), and the context of recent agricultural policies history in OCED countries is referenced to gain this goals in Agricultural policies.