原發性 後復膜腫瘍 28例에 對한 臨床的 考察

鄭相鳳(Sang Bong Chung),權宏保(Koing Bo Kwun),鄭在龍(Jae Ryong Jung),朴永寬(Young Kwan Park),李鐵(Chul Lee) 대한외과학회 1970 Annals of Surgical Treatment and Research Vol.12 No.8

진단이 늦어진 기관삽관 후 발생한 턱관절 탈구

정상봉 ( Sang Bong Chung ),전형배 ( Hyoung Bae Jeon ),김태관 ( Tai Kwan Kim ) 대한외상학회 2015 大韓外傷學會誌 Vol.28 No.2

Mandible dislocation during endotracheal intubation is an unusual occurrence but easy to be overlooked for its unfamiliarity. We recently had a case of iatrogenic bilateral dislocation of a temporomandibular joint during orotracheal intubation and emphasize the importance of an early awareness of this possible complication. [ J Trauma Inj 2015; 28: 75-78 ]

미만형 위암에서 임상병리학적 인자와 Hsp70, BAG1과 Raf-1 발현간의 상관성

정상봉(Sang Bong Jung),이현욱(Hyoun Wook Lee),정경태(Kyung Tae Chung) 한국생명과학회 2016 생명과학회지 Vol.26 No.1

본 연구에서는 미만형 위암조직에서의 임상병리학적 인자와 HSP70, BAG1, Raf-1의 발현간의 상관관계를 평가하고자 하였다. Hsp70은 열충격단백질(heat shock protein)의 환경적 스트레스로부터 세포를 보호하는 분자생물학적 chaperone으로 작용하는 것뿐 아니라 anti-apoptotic 작용을 통하여 암세포의 생존과 전이를 촉진시키는 것으로 알려져 있다. Proto-oncogene의 일종인 Raf는 사람을 포함한 진핵세포의 생장에 관여하는 중요한 신호전달경로인 mitogen-activated protein kinase (MAPK)경로의 중심인자로서 다양한 암에서 그 발현이 증가한다고 알려져 있다. Bcl-2에 결합하여 세포사멸을 저해하는 것으로 처음 보고된 Bcl-2-associated athanogene-1 (BAG-1)은 다양한 암에서 그 발현이 증가된다고 보고되었다. 또한 BAG-1은 HSP70과 상호작용하여 Raf-1-ERK signal pathway와 cell growth를 조절하는 것으로 보고되었다. Hsp70의 발현은 임파절 전이가 있는 경우 24례(64.9%)에서 양성, 13례(35.1%)에서 음성을 보였으며, 임파절 전이가 없는 경우 6례(26.1%)에서 양성, 17례(73.9%)에서 음성을 보여 임파절의 전이가 있는 경우가 임파절 전이가 없는 경우에 비해 유의하게 높은 발현율을 보였다(p=0.007). N-stage에 따른 발현은 N-stage가 증가함에 따라 발현율이 유의하게 증가하였다(p=0.006). BAG1의발현은 전체 대상 환자 중 43례(71.7%)에서 양성, 17례(28.3%)에서 음성소견을 보였으며, 65세 미만에서 65세 이상에 비해 유의하게 높은 발현율을 보였다(p=0.035). RAF1의 경우 전체 대상 환자 중 45례(75.0%)에서 양성, 15례(25.0%)에서 음성소견을 보였으며, BAG1 양성환자에서 RAF1의 발현이 유의하게 증가 하였다(p=0.021). 본연구 결과 Hsp70은 종양의 진행과 상관성이 있을 것으로 생각되어지며, BAG1과 Raf-1은 미만형위 암의 진단적 마커로 유용할 것으로 사료된다. The aim of this study was to evaluate the relationships between the expression of Heat shock protein70(HSP70), Raf-1 and Bcl-2-associated athanogene-1 (BAG1) protein in diffuse type gastric carcinoma and examine association of HSP70, Raf-1 and BAG1 expression with various clinic-pathological factors and survival. Heat shock protein70 is induced in the cells in response to various stress conditions, including carcinogens. Overexpression of heat shock protein 70 has been observed in many types of cancer. The proto-oncoprotein Raf is pivotal for mitogen-activated protein kinase (MAPK) signaling, and its aberrant activation has been implicated in multiple human cancers. Overexpression of BAG1 protein has been documented in some type of human cancer. BAG1 has been reported to interact with protein involved with a variety of signal pathway, and regulation of cell differentiation, survival and apoptosis. These interaction partners include HSP70 and Raf-1. The percentage of tumors exhibiting HSP70 positivity was significantly in cases of positive lymph node metastasis (64.9%) compared to cases without lymph node metastasis (35.1%, p=0.007). HS70 expression was correlated with pathological N-stage (p=0.006). Expression of BAG1 was detected in the majority of diffuse type gastric carcinoma tissues (71.7%), especially in younger patients (80% vs 52.6%, p=0.035). Furthermore BAG1 expression was correlated with tumor size (p=0.020). Raf-1 expression was found to be significantly associated with tumor size (p=0.005). The result indicate that HSP70 was significantly correlated the progression of diffuse type gastric cancer. Expression of BAG1 and Raf-1 may be used as diagnostic markers for gastric carcinoma.

네트워크기반 로봇 축구 시스템

조동권,정상봉,성영휘,Cho, Dong Kwon,Chung, Sang Bong,Sung, Young Whee 대한임베디드공학회 2009 대한임베디드공학회논문지 Vol.4 No.1

In this paper, a network based robot soccer system is proposed. The system consists of robots, an image processing sub-system, a game server, and client systems. Embedded technique is applied to the hardware and software for controlling the robots and image processing. In this robot soccer system, a gamer can see and control robots in a remote site through Internet. During the game, the game server gives geometrical information on robots such as positions and orientations. We demonstrated the game in public and obtained optimistic results even though some technical problemssuch as communication delay and precise control for the robots should be improved.

면역세포화학적 방법을 이용한 고양이 중간뇌에서 Calbindin D-28k 와 Parvalbumin을 함유한 GABA의 분포상에 관한 연구

김종중(Jong-Joong Kim),정상봉(Sang-Bong Jeong),김남훈(Nam-Hoon Kim),안계훈(Gye-Hoon Ahn),정윤영(Yoon-Young Chung),문정석(Jeong-Seok Moon),장인엽(In -Youb Chang),김홍순(Hong-Soon Kim) 대한체질인류학회 2000 대한체질인류학회지 Vol.13 No.2

간추림 고양이 중간뇌에서 calbindin D-28k (CB) 와 parvalbumin (PV), 그리고 gamma-aminobutyric acid (GABA) 의 분포상과 세포의 형태 및 크기를 관찰하고, 동일세포 E 안에서 이들의 공존을 확인하기 위하여 본 실험을 시행 하였다. CB, PA 및 GABA 에 대한 특이성을 지난 단세포군항체를 이용한 면역세포화학적 염색을 하여 관찰한 결과 다음과 같은 결론을 얻었다. 1 적색핵, 흑색질, 눈돌림신경핵, 청색핵에서 CB-IR, PV-JRGABA-IR 세포를 가장 많이 관찰할 수 있었다. 2 적색핵에서는 PV-IR 세포가, 흑색질에서는 CB-IR 세포가 더 많이 출현하였다. 3. PV-IR 과 GABA-IR 이 통일세포에 공존하는 것을 관찰할 수 있었다. 4. CB-IR, PV-IR, GABA- IR 세포의 세포체의 형태는 방추형, 타원형, 원형이었고 그 크기는 15-20 μm 이었으며 세포의 모양을 흩극, 이극, 뭇극신경원이었다.

흰쥐 편도체에서 칼슘결합단백인 calbindin-D28K함유 신경세포의 생후 발달에 관한 면역조직학적 연구

정윤영,김종중,문정석,박영란,정상봉,김현곤,박상수 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and Objectives : The calcium-binding protein, calbindin-D28K(CB) is widely distribution of CB in rat amygdala during postnatal maturation, in order to provide an insight into the developmental role it may play in the amygdaloid neurons. Materials and Methods : The present study is based on 28 postnatal cases ranging from the day of birth, postnatal day 0 (P0) to 30 days (P5, P10, P15, P20, P30), and these cases were compared with adult rat amygdala. Cryostat sections processed free-floating with monoclonal antibody by immunohistochemistry. Results : CB-immunoreactive neurons were already found at birth in all amygdaloid nuclei except the intercalated nucleus. The intensity and number of immunoreactivity of CB-containing neurons increased during the first 10 days of postnatal life but dramatically decreased at P15. Mature patterns of CB-immunoreactive neurons were achieved at P20. In the adult amygdala, CB-immunoreactive neurons were distributed in almost all amygdaloid nuclei except the intercalated nucleus. Conclusion : The present results show early appearance of CB immunoreactivity in the amygdala. It may be related to the early appearance of CB immunoreactivity in many other brain regions, and suggests that CB may play a functional role during the development of the nervous system including amygdala.

Ethionine投與로 因한 肝細胞의 蛋白合成機構들의 變化

鄭相鳳,金秉憲,洪錫宰 慶北大學校 醫科大學 1976 慶北醫大雜誌 Vol.17 No.2

치자(Fructose gardeniae)의 수용성 추출액(Crocin색소)이 괴�성 세균의 증식에 미치는 영향

봉만전,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1983 中央醫大誌 Vol.8 No.4

In our previous study, it was found that some of bacteria grown on agar medium containing soluble extracts of Fruclose gardeniae(refered to corcin components) producted dark violet pigment around their colonies, and development of the colonies on the medium was slightly enhanced. It was presumed that the corcin components did contain some growth factors for some of bacteria. In the present study, to determine the presence of growth factor in the crocin components, growth curves of bacterial concentration at early stage of exponential growth of Brucella abortus, Bordetella pertussis, Mycobacterium bovis and Propionibacterium acnes in the broth medium were measured in the presence or absence of crocin components. In addition, development of bacterial colonies of rapidly or moderate rapidly growing strains of M. fortuitum-chelonei complex on Middlebrook 7H10 agar medium with or without crocin components was compared. The results are summarized as follows: Bacterial growths of B. abortus and M. bovis in the broth were markedly enhanced by the crocin components, whereas growths of neither P. acnes nor B. pertussis were affected by the crocin components. Development of bacterial colonies of M. fortuitum-chelonei complex on Middlebrook 7H10 agar medium with corcin components were also enhanced. Visible colonies of M. fortuitum-chelonei complex on 7H10 agar medium with corcin components appeared 24 to 48 hr earlier than without crocin components. These results indicated that the crocin components might be of value for the cultivation of some fastidious bacteria in their culture.

Ethionine投與로 因한 肝細胞의 蛋白合成機構들의 變化

鄭相鳳,金秉憲,洪錫宰 慶北大學校 醫科大學 1976 慶北醫大誌 Vol.17 No.2

저자는 흰쥐에 1.25% DL-ethionine은 4㎖를 단회 복강내 주입한 초기 실험군과, 기본식에 DL-ethionine을 0.25% 첨가한 사료로 3개월 및 6개월간 사육한 다음 도살한 군의 간조직에 일어나는 변화를 관찰하고 얻는 결과를 요약하면 다음과 같다. Ethionine을 복강내로 투여하면, rER의 확장, polysome의 감소, Golgi complex의 위축 등의 퇴행성 변화가 야기되고, 이들 변화는 시간이 지남에 따라 점점 심해지는 경향이었으며, 장기간 식이로 투여한 군에서는 상기한 단백합성 저해의 소견과 더불어, 각종 형태의 lysosome이 증가하였다. 이상의 연구성적으로 보아 ethionine으로 인한 간세포상해는 rER의 확장 및 그막으로부터의 ribosome의 탈락 그리고 free polysome의 감소로부터 시작하여, 이 변화는 Golgi complex로 파급되어 심한 세포의 상해의 결과, 마침내 lyosome의 증가를 초래하는 것이라 생각된다. The author studied the effects of ethionine adminstration on the intracellular fine structures of liver cell, focusing on the organelles involving protein synthesis. In one group of animal, rats were received intraperitoneal injection of 1.25% DL-ethionine and sacrificed at 30 minutes, I hour after injection respectively. Another group animals were feeded with ethionine for 3 months and 6 months and sacrificed. The livers were extracted out and observed under electron microscope. The results were summarized as follow; Degenerative changes of the organelles induced by intraperitoneal injection of ethionine were characterized by dilatation of rER, decreased number of polysome and atroph of Golgi complex. These changes were already evident than the group of animal which were sacrificed at 30 minutes after treatment and become progressively more severe with prolonged time interval between treatment and sacrifice, Longterm ethioinine feeded group showed increased number of various lysosome in addition to the above mentioned changes of inhibited protein synthesis. The results suggested that liver cell injury induced by ethionine were initiated from dilatation of rER, shedding of ribosome and decreased number of free polyribosome. And these changes were extended to the Golgi complex and resulted to increased number of lysosome.

어렝놀래기, Ptergogus flagellifera(Valenciennes)의 生殖周期와 性轉換

李榮敦,鄭相喆,高有峰 濟州大學校海洋硏究所 1992 해양과환경연구소 연구논문집 Vol.16 No.-

1990년 10월부터 1991년 9월까지 그리고 1992년 7월-10월까지 제주도 북방함덕연안을 중심으로 채집된 어렝놀래기, Pteragogus flagellifera를 대상으로 그들의 生殖生態를 파악하기 위하여 體色變化,生殖巢 熟度指數, 그리고 生殖巢의 組織學的 방법에 의한 生殖週期, 性轉換過程들을 조사한 결과는 다음과 같다. 1. 生殖巢 熟度指數(GSI)는 수컷이 6월에 0.62, 암컷은 7월에 10.15로 최대값을 나타내고 있다. 그리고 암수 다같이 8월에 접어들면서 下降하기 시작하면서, 9월부터 낮은값을 나타내고 있다. 2. 生殖週期는 成長期(5∼6월), 成熟期(6∼7월), 完熟 및 産卵期(7∼8월), 回復 및 休止期(8∼4월)등의 연속적인 週期를 나타냈다. 3. 體長 9.5∼11.5㎝사이에 14개체, 體長 13.0∼15.0㎝사이에 3개체가 雌性에서 雄性으로 性轉換過程의 生殖巢를 가지고 있다. 4. 암컷에서 수컷으로 性轉換함에 따라 적갈색에서 흑녹색으로 體色變化가 나타나고 있다. 5. 본 種은 암컷이 性轉換 하여 2차精巢를 갖는 單雄性의 雌性先熟魚로 간주된다. Reproductive cycle and sex reversal of the cock-tail wrasse, Pteragogus flagellifera, were investigated histologically on the gonadal development, sex change of the gonad and studied by gonadosomatic index(GSI),color patterns. Samples were collected at the costal area of Hamduck in Cheju-do, from october, 1991 to october, 1992. In male and female, GSI began to increase from May(male) and June(female) when the water temperature began to increase and reached it's maximum value in Jun(male) and July(female). It began to decrease from August, thereafter, maintained relatively low value until May of nex year. The annual reproductive cycle of this species could be classified into four successive development stage : growing stage(May-June), mature stage(June-July), ripe and spent stage(July-August), degenerative and resting stage (August-April). The species is a protogynous hermaphrodite, and has two color patterns:red-yellow(A) and dark-green (B). A-fish were less than 12.0㎝ SL and consisted of female (74.4%) and fish with transitional gonad (25.6%). B-fish consisted of secondary male (87.3%) and fish with transitional gonad (12.7%). In P.flagellifera, individuals with hermaphrodetic gonad were observed in 8.0∼12.0㎝ SL. Sex reversal of this species could be occured from 8.0∼12.0㎝ SL.