사람 腦를 使用한 Thromboplastin 製造에 關한 實驗的 硏究

鄭在泓,宋文源 계명대학교 醫科大學 病理學敎室同門會 1987 樂山 鄭在泓 敎授 頌喜 紀念論文集 Vol.S No.-

1. The author has tried to prepare a standard thromboplastin for the determination of prothrombin time utilizing relatively fresh child human brain obtained at the time of autopsy, through acetone treatment. 2. A 13. 5 second thromboplastin extract, slightly slower than. the 12 second commercially available Difco product, was successfully prepared. 3. No influence on prothrombin time is’ noted when the thromboplastin solution is extracted from the powdered thromboplastin at the temperature, between 45 0C and 550 C. However, definite prolongation is observed when the extraction is made at above 600 C. 4. It appears that the optimal concentrations of powdered thromboplastin for the preparation of thromboplastin solution lie between 300 mg and 500 mg. 5. An attempt was made to prepare a slower acting thromboplastin sensitive at the level of therapeutic range, using rabbit brain and lung extract in equal parts, with uncertain success. 6. It is felt that the prospect of preparing a satisfactory fast acting standard thromboplastin is good, judging from the knowledge acquired through this experimentation. At this time, .however, we are unable to draw any specific conc1usion concerning the preparation of a sensitive slower acting thromboplastin from the rabbit.

血中 Ammonia 微量定量에 있어서 pH 및 Alkali 媒劑가 測定値에 미치는 影響에 對한 硏究

鄭在泓 계명대학교 醫科大學 病理學敎室同門會 1987 樂山 鄭在泓 敎授 頌喜 紀念論文集 Vol.S No.-

An attempt was made to determine the origin of artifactual blood ammonia formed in the shed blood; Experimentation, with blood specimens from more than 400 healthy individuals, was carried out over a period of four and a half years, utilizing two popular microdiffusion blood ammonia methods, those of Seligson and Conway. The actions of several alkali [pstassium carbonate, carbonate-bicarbonate mixture and borate-sodium hydroxide (with a pH of 9 to 11.4)] were compared by placing them in the diffusion apparatus with specimens of blood. It was found that the ammonia concentration tended to be higher with increasing pH and vice versa lowering with decreasing pH. While occasional extremely implausible results were obtained in experiments using the potassium carbonate and carbonate-bicarbonate mixture, on the other hand, no such aberrations were observed with borate-sodium hydroxide. Within the pH range, from 9 to 10.1, the yield of ammonia when plotted against the pH of the blood-alkali mixture showed a straight line. Increasing beyond 10, resulted in an asymptomatic character of diffusion curve. For these reasons, borate-sodium hydroxide reagent which results in a pH of 10.1 in the presence of blood, is selected as the choice of weak alkali media for the determination of true blood ammonia. The author has to abandon his earlier thought that aberrations in ammonia analyses can be largely attributed to faulty technique in collecting and handling blood. Careful control of these techniques resulted in some lowering of the ammonia concentration, but failed to prevent the more serious aberrations encountered. In search for clarification of the origin of extra ammonia, studies were carried out by experimenting with glutamine, both in aqueous solutions and following mixture with blood. The results suggest that non-protein glutamine is an unlikely source of extra ammonia. The only other plausible source of the extra ammonia liberated from blood, therefore, seemed to be the blood proteins, Accordingly, the action of various alkaline reagents on plasma albumin, globulin and on whole blood was tested, after prolonged dialysis to remove preformed ammonia. Ammonia was released from each of the protein solutions used. However, the action of borate in this release was less pronounced than that of the carbonate-bicarbonate mixture. This latter mixture, in turn, liberated substantially less ammonia than did potassium carbonate. Therefore, it is concluded that the origin of artifactual ammonia may be traced to the decomposition of protein by the action of alkali. The author has revised the method of measuring blood ammonia in such a way as to avoid spontaneous production of ammonia from blood proteins. The technique involves the use of sodium borate buffer at a pH of 10.8±0.2, which, when mixed with blood, results in a pH of 10.1 The adequacy of this technic is well proven by the satisfactory results obtained in a large number of recovery studies. The technique described, applied to venous specimens that were collected in EDTA tubes and promptly analyzed, was used to study a group of 16 healthy persons, 20 to 25, male, with no history of previous liver involvement. The mean blood ammonia was 0.42 μg./ml. expressed as N. The lowest value was 0.21, the highest, 0.61, with standard deviation of ± 0.124.

李奎報의 假傳體文學攷

鄭在洪 효성여자대학교 국어국문학연구실 1959 국어국문학연구논문집 Vol.8 No.-

크롬親和性細胞腫의 超微形態學的 觀察

權建營,鄭在泓 啓明大學校 醫科大學 1983 계명의대학술지 Vol.2 No.2

저자들은 최근 전형적인 임상증상을 보이는 크롬친화성세포종 2례를 경험하여 육안적소견 및 광학현미경적 소견을 자세히 검토하고 이중 1례(S83-3304)에서는 외과적으로 적출한 종괴도부터 전자현미경적 시료를 채취하여 1㎣로 세절한 다음 전자현미경 시료제작의 각 과정을 거쳐 uranyl acetate와 lead nitrate로 이중전자염색을 한 다음 Hitachi H-600형 전자현미경을 통하여 관찰하고 한편으로는 chromaffin 세포내에 존재하는 catecholamine 과립들의 크기를 자세히 알기 위해 형태계측기인 AIM 65형 Graphic Data Image Analyzer(Ladd, USA)를 사용하여 Catecholamine 과립들의 크기를 측정한 결과는 다음과 같다. 전자현미경적으로 chromaffin 세포들은 그 세포질에서 많은 돌기를 내어 인접 세포의 세포질 속으로 함입하는 양상을 흔히 볼 수 있으며, 핵은 비교적 크고 뚜렷한 핵소체를 가지며 이염색질(heterochromatin)의 덩어리가 핵막 내면쪽으로 붙어있는 양상을 볼 수 있었고, 한편 세포질 내에는 다양한 크기의 epinephrine 과립과 noreepinephrine과립들이 산재되어 있으며 이들은 모두 한계막에 의해 싸여져 있었는데 이들 두가지 형태의 과립들은 Johannessen, Shin 등과 Brown 등이 기술한 과립들의 형태와 일치하였으며, 또한 몇몇 과립들은 중심핵이 없고 한계막은 늘어나 공포와 같은 구조를 볼 수 있으며 이 밖에 세포질 내에는 무수한 mitochodria와 잘 발달된 Golgi 장치 그리고 freeribosomes과 소량의 glycogen 입자들도 관찰할 수 있었다. 형태계측학적 소견으로 catecholamine 과립들은 그 크기가 다양하며 그 직경은 대부분 100㎚에서 600㎚에 이르렀으며 숫적으로 또한 크기에 있어서 norepinephrine 과립들이 epinephrine 과립들보다 많고 컸으며 그 평균치를 산정하였을 때 norepinephrine 과립들이 325㎚였으나 epinephrine 과립들은 281㎚로 측정되었다. 한편 가장 큰 과립은 764㎚였으며 가장 작은 과립은 31㎚ 이들은 모두 epinepirine을 포함하는 과립들이었다. Experienced were two cases of functional pheochromocytoma of adrenal glands with pertinent clinical findings. An electron microscopic study was performed on one case (S83-3304) with particular reference to delineation of chromaffin granule specificity and that of type of catecholamine content. Also measurement was made for the dimension of the catecholamine granules, using a morphometric instrument, the graphic image analyzer, AIM 66 (Ladd, USA). Results observed were summarized as follows: 1. LIght microscopically, case 1 (S83-3304) showing clusters and cords of cells with abundant granular eosinophilic cytoplasm. These tumor cells were separated by thin bands of connective tissue with plentiful thin-walled blood vessels in close proximity to tumor cells. Their nuclei were ovoid and medium-sized. Occasional hyperchromatic and degenerating forms. Case 11 (S83-6514 0 was much more aggressive and atypical in that this tumor showing irregular and hyperomatic nuclei with occasional tumor giant cells to the extent to be suggestive of anaplastic neoplasm. 2. Electron microscop ically, the neoplastic chromaffin cells were polymorphic with numerous processes interdigitating with invagination into the adjacent cells. The large nuclei with prominent nucleoli revealed coarse heterochromatin clumps attached to the inner-aspect of nuclear membrane. In the cytoplasm, catecholamine granules with varying size were scattered and each was surrounded by limiting membrane. The electron dense portion of each epinephrine granule was composed of the finest subparticles which varied in diameter, from 50 to 100A˚. Whereas norepinephrine granules were quite different from epinephrine granules, in that there noted a clear space or halo interposing between the limiting membrane of the granules and the electron-dense core. Also the core of norepinephrine granules was more electron-dense and no fine granularity as noted in the case of epinephrine granules. Also present in the cytoplasms were numerous mitochondria, well-developed Golgi apparatus and some free ribosomees. 3. Morphometrically, the catecholamine granules had variable sizes, mostly from 100 to 600 nm in dimension. Norepinephrine-containing granules were larger than epinephrine-containing granules. Mean diameter of norepinephrine granules was 325 nm and that of the epinephrine granules being 281nm. The largest granule was 764nm and the smallest granule, 31nm, all of which belonged to the bracket of epinephrine-containing granules.

Relevant Reduction Effect with a Modified Thermoplastic Mask of Rotational Error for Glottic Cancer in IMRT

정재홍,정주영,조광환,류미령,배선현,문성권,김용호,최보영,서태석 한국물리학회 2017 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.70 No.3

The purpose of this study was to analyze the glottis rotational error (GRE) by using a thermoplastic mask for patients with the glottic cancer undergoing intensity-modulated radiation therapy (IMRT). We selected 20 patients with glottic cancer who had received IMRT by using the tomotherapy. The image modalities with both kilovoltage computed tomography (planning kVCT) and megavoltage CT (daily MVCT) images were used for evaluating the error. Six anatomical landmarks in the image were defined to evaluate a correlation between the absolute GRE () and the length of contact with the underlying skin of the patient by the mask (mask, mm). We also statistically analyzed the results by using the Pearson’s correlation coefficient and a linear regression analysis (P < 0.05). The mask and the absolute GRE were verified to have a statistical correlation (P < 0.01). We found a statistical significance for each parameter in the linear regression analysis (mask versus absolute roll: P = 0.004 [P < 0.05]; mask versus 3D-error: P = 0.000 [P < 0.05]). The range of the 3D-errors with contact by the mask was from 1.2% - 39.7% between the maximumand no-contact case in this study. A thermoplastic mask with a tight, increased contact area may possibly contribute to the uncertainty of the reproducibility as a variation of the absolute GRE. Thus, we suggest that a modified mask, such as one that covers only the glottis area, can significantly reduce the patients’ setup errors during the treatment.

맛닭을 이용한 오색삼계탕의 관능검사비교

정재홍,최희숙,장정옥,김형렬,양미옥 한국식품영양학회 2011 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2011 No.12

두경부암 토모테라피 방사선치료에서 Aid-pillow 사용에 따른 pitch와 yaw의 변화 분석

정재홍,조광환,김용호,문성권,민철기,김은석,이경배,정주영,서태석,최보영 대한방사선방어학회 2013 대한방사선방어학회 학술발표회 논문요약집 Vol.2013 No.04

기능성과 다양한 조리법에 따른 맛닭 삼계탕의 관능검사비교

정재홍,최희숙,장정옥,김형렬,양미옥 한국식품영양학회 2011 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2011 No.12

白鼠의 無機質代謝에 미치는 칼슘과 마그네슘의 影響 : 2. Effect on the sodium, potassium, copper and iron metabolism in tissues 2. 組織 중의 Na, K, Cu 및 Fe 代謝에 미치는 影響

曺秀悅,鄭在洪 嶺南大學校 環境問題硏究所 1982 環境硏究 Vol.2 No.1

This experiment was undertaken to investigate the effect of the diet containing two levels of calcium(0.5%, 0.1%) and magnesium (0.04%, 0.0002%) on the sodium, potassium, copper and iron concentrations of kidney, liver and femur in rats. Thirty-two males of Sprague-Dewley strain, 70-80g, were divided into 4 groups and each group(8 rats)was fed ad libitum for 4 weeks. sodium and potassium in the liver, kidney and femur were determined by flame spectrophotometry. Copper and iron in liver, kidney and femur were determined by atomic absorption spetrophotometry. The results obtained are summarized as follow. Net weight gain and feed efficiency ratio were decreased in the calcium and magnesium-deficient groups. The weight of liver, heart and kidney generally showed increasing tendency in the calcium-deficiency group. The weight of iung was showed to decreased in the magnesium-deficient group compared to the control group. The sodium and potassium concentrations of liver, kidney, and femur are the dietary calcium and magnesium levels did not tend to affect on the sodium concentration of kidney and potassium concentration fo femur and liver. But sodium content of liver tended to be significantly increased in the magnesium deficient-group. The potassium concentration of kidney in the deficient-groups(II,III,IV) tended to be greatly increased as compared with the control group. The sodium concentration of femur in the deficient-groups(II, III, IV) was slightly lower as comapared with that of the control group. Both copper and iron contents of the kidney, liver, and femur were the highest in kidney and the dietary Ca and Mg levels were not effected on the copper content of liver. The iron concentration of liver in the Ca-deficient group tended to be greatly higher than that of the other groups. The copper contents of kidney in the Mg-deficient group was lower than that of the other groups, and the iron contents in the deficient groups(II, III, IV) lower as compared with the control group. The copper content of the femur tended to be lower at the deficient levels(II, III, IV) and the copper concentration at the Mg-deficient level was greatly increased as compared with that of the control group.

칼슘의 첨가에 따른 라면의 조직감과 관능적 특성

정재홍 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.3

칼슘의 첨가가 밀가루의 아밀로그래프에한 호화 성질과 파리노그래프에 의한 반죽 성질을 조사하였고, 칼슘을 첨가하여 제조한 라면의 조직감과 관능적 특성에 미치는 영향을 검토하기 위하여 밀가루에 대하여 1.0%∼3.0%를 사용하여 라면을 제조한 뒤 면의 조직 특성, 조리시험을 측정하고 관능검사를 평가하였다. 칼슘의 첨가는 아밀로그래프의 호화개시 온도를 지연시켰으며, 최고 점도를 감소시켰다. 패리노그래프의 흡수율과 반죽의 안정도는 칼슘의 첨가에 의해 증가되었다. 칼슘을 첨가하여 제조한 라면의 층밀림 압출력과 경도는 대조구보다 높게 측정되었다. 3.0%의 칼슘을 첨가하여 제조한 라면의 무게 증가는 대조구 보다 높게 나타났으나, 부피 증가는 오히려 낮게 나타났다. 용출량의 변화는 칼슘의 첨가량에 따라 약간 감소하는 경향을 보였다. 요오드 정색도는 모든 시료가 2.12∼2.22으로 유사하게 나타났다. 칼슘을 첨가하여 제조한 라면의 관능 검사 결과는 비교적 좋은 점수를 얻었다. 이 결과에서 알 수 있듯이 라면 제조시 칼슘의 첨가량은 3.0%의 수준이 효과가 큰 것으로 평가되었다. In an attempt to evaluate the effects of calcium on paste or gelatinization properties by amylograph and mixing properties by farinograph of wheat flour, and on viscosity property, cooking quality, textural and sensory properties of Ramyon were examined. The contents of calcium used were from 1.0% to 3.0% based on flour weight. The viscosity property of wheat flour with calcium was increased the initial pasting temperature but the amylograph peak viscosity were decreased in vice versa. The farinography absorption, stability and breakdown were increased by calcium. The shear extrusion force and hardness of Ramyon manufactured with calcium were shown much higher value than those of control. At cooking quality examination of Ramyon manufactured with calcium, weight of cooked Ramyon was increased but volume was decreased . Extraction amounts of Ramyon manufactured with calcium during cooking were much smaller than those of control. These changes will provided many advantages in the preparation of Ramyon. The I_2 reaction value of Ramyon manufactured with calcium and control were shown to almost same values. Sensory properties of cooked Ramyon which was manufactured with calcium showed quite acceptable. Based on the cooking and sensory evaluation test, addition of 3.0% calcim to wheat flour may be suitable for processing Ramyon.