白鼠의 血淸, 硬骨 및 齒牙의 無機成分에 미치는 카드뮴의 影響

조수열(Soo Yeul Cho),안춘길(Choon Kil Ahn),정재홍(Jae Hong Jeung) 한국식품영양과학회 1983 한국식품영양과학회지 Vol.12 No.3

無機質 相互間에 미치는 영향을 檢討코저 CdCl₂ 溶液을 飮料水(0, 5, 10, 20, 40ppm)로하여 白鼠에게 4週間 投與하였을 때 血液, 硬骨 및 齒牙 중의 無機成分含量을 測定 考察한 結果는 다음과 같다.<br/> 1. Cd給與水準이 높아짐에 따라 현저한 體重減少와 낮은 飼料效率을 나타내었다.<br/> 2. 單位體重 당 臟器重量은 肝臟이 增加했는 반면에 다른 臟器들은 아무런 영향을 받지 않는다.<br/> 3. 血淸 중의 Cd含量은 40ppm群에서 현저하게 높았으나, Fe, Cu, Zn 및 Mg含量은 減少하였다.<br/> 4. 硬骨 중의 Cd含量과 P含量은 40ppm群에서 많아졌으나, Fe, Cu, Zn, Mg, Ca含量 및 灰分含量은 Cd給與水準을 높일수록 減少하는 傾向을 나타내었다.<br/> 5. 齒牙 중의 Cd含量은 血液과 硬骨에서 보다 훨씬 많았으며, Fe, Cu, Zn 및 P含量은 Cd給與水準을 높일수록 減少하는 傾向을 나타내었고, Mg含量과 灰分含量은 增加하는 傾向을 나타내었다. This experiment was undertaken to determine the effect of several levels of cadmium on growth and some mineral concentrations in the serum, tibia and teeth of rats.<br/> The results obtained are summarized as follows.<br/> Growth rate was significantly decreased as the feeding level of cadmium increased, especially body weight in the 40 ppm level was greatly reduced than that of initial body weight.<br/> The weight of liver was increased in 40 ppm group as a result of edema. But the weight of kidney, heart, spleen, lung and testis was not affected.<br/> The cadmium concentration of serum was higher in rats receiving 40ppm than that of other levels, and the concentrations of iron, zinc, copper, magnesium and calcium were decreased as the cadmium content of drinking water increased.<br/> The cadmium and phosphorus contents of tibia were greatly high in the 40ppm level, but the content of iron, copper, zinc, magnesium, calcium and ash tended to be decreased with increasing level of cadmium.<br/> The cadmium concentration of teeth was predominantly high compared with that of the serum and tibia. Also, the concentration of iron, copper and zinc tended to be dedrased with increasing cadmium level, but the contents of magnesium, calcium and ash tended to be increased.

Chlorella ellipsoidea 엽록체의 인지질 생합성 및 지방산 조성에 미치는 항생제의 효과

조수열(Soo Yeul Cho),이종삼(Chong Sam Lee) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.1

The effects of amphotericin B (150 ㎍/㎖) and cycloheximide (10 ㎍/㎖) on the biosynthesis of phospholipid and the composition of fatty acids in chloroplasts isolated from Chlorella were analyzed. The contents of the total lipid and phospholipid (PC, PE, PI) in treatment with antibiotics were lower compared with the control. In the whole cell system, the major fatty acids utilized for biosynthesis of phospholipid were palmitic acid (31.96%) and linoleic acid (16.96%) in control while those were palmitic acid (36.15%) and linolenic acid (16.71%) in treatment with amphotericin B. And in treatment with cycloheximide, palmitic acid (31.90%) and stearic acid (15.32%) were used in phospholipid formation. The major fatty acids in chloroplasts were analyzed as to be palmitic acid and linolentic acid in control (33.75%, 18.90%) and in treatment with amphotericin B (36.75%, 9.46%). However, it was shown that the major fatty acids in chloroplasts treated with cycloheximide were palmitic acid (28.01%) and oleic acid (19.27%).

콜레스테롤 식이로 사육한 흰쥐의 혈청 및 간지질 성분에 미치는 카페인 및 비타민 E의 영향

조수열(Soo-Yeul Cho),박미리(Mi-Lee Park),박원학(Won-Hak Park),정재홍(Jae Hong Jeong) 한국식품영양과학회 1989 한국식품영양과학회지 Vol.18 No.1

카페인과 비타민 E가 체내 지질대사에 미치는 영향을 검토코저, 콜레스테롤 첨가 식이에 카페인(0.25%), 비타민 E(400㎎/㎏ diet) 및 카페인+비타민 E를 각 군에 따라 급여하고 6주간 사육한 후, 흰쥐의 성장, 각종 장기중량, 혈청 및 간중의 지질성분을 측정하고 아울러 간조직검사를 행한 결과는 다음과 같다.<br/> 체중증가량은 CF군만이 유의하게 낮았다. 간 무게는 Basal+Chol.군에 비해 전 실험군에서 유의적인 차이가 없었으며, 신장의 경우 CF군과 CF+VE군에서 유의하게 증가하였다. 혈청중의 총지질은 VE군과 CF+VE군에서 감소하였다. 총 콜레스테롤 및 HDL-Cholesterol은 CF군에서 증가하였으며, 트리글리세리드(TG)는 Basal+Chol. 군에 비해 CF군, VE군 및 CF+VE군에서 유의하게 낮았다. 간중의 조지질은 VE군, CF+VE군에서 감소하였고, TG함량은 VE군, CF+VE군 및 CF군에서 감소하였다. 한편 간조직검사는 Basal+Chol.군이 미세한 과립상의 지방소적으로 팽창된 데 비해 VE군 및 CF+VE군에서 비교적 호전되었으며, CF군 및 CF+VE군에서 비교적 호전되었으며, CF군의 경우 지방소적의 증가와 동양혈관의 확장이 관찰되었다. This experiment was undertaken to investigate the effect of caffeine and vitamin E on the lipid composition of serum and liver in cholesterol-fed rats. Thirty-five male rats of Sprague-Dawley strain weighing about 120±10 g were divided into 5 groups, each group receiving a different diet for 6 weeks. i.e. basal diet (Basal group), basal diet supplemented with 1.0% cholesterol (Basal+Chol. group), basal diet supplemented with 1.0% cholesterol and 0.25% caffeine (CF group), basal diet supplemented with 1.0% cholesterol and 400ppm vitamin E (VE group), and basal diet supplemented with 1.0% cholesterol and caffeine plus vitamin E (CF+VE group).<br/> Net weight gain was significantly depressed only by caffeine supplementation. Serum total lipid was significantly decreased in VE and CF+VE groups. Serum total cholesterol and HDL-cholesterol were slightly increased by caffeine supplementation, whereas triglyceride (TG) was significantly decreased in CF, VE and CF+VE group. Contents of crude lipid and TG in liver were significantly reduced by supplementation of vitamin E. No differences in liver cholesterol were found among experimental groups. Histologically, Basal+Chol. group showed a spotty necrosis, including a profuse fine microvaculoar lipid accumnlation. CF group exhibited a mild inflammatory infiltration, as well as macro- and microvacuolar lipid accumulation. But those were regenerated by supplementation of vitamin E.

차전자수침액이 흰쥐의 체내 지질대사에 미치는 영향

조수열(Soo-Yeul Cho),김명주(Myung-Joo Kim) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.4

차전자 성분이 지질대사에 미치는 영향을 알아보기 위해서 흰쥐에 lard를 다량 첨가한 식이를 급여한 후 차전자수침액을 투여하여 성장시킨 후 체중, 장기 중량 및 지질 함량을 측정하였다. 고지방식이군과 차전자수침액 투여군의 체중은 별 차이가 없었으나 이를 병행 투여할 경우 대조군에 비하여 유의적인 증가를 보였으며, 식이효율은 고지방식이를 급여한 BL과 BLP군이 대조군인 BD군에 비하여 유의적 증가를 나타내었다. 각 장기의 중량은 식이 차이에 따른 유의적 변동을 나타내지 않았다. 혈청과 간조직 중의 콜레스테롤 함량은 고지방식이 급여시 대조군에 비하여 유의적인 증가를 보였으며, 차전자수침액을 병행 투여할 경우 유의적인 감소를 나타내었다. 인지질과 HDL-콜레스테롤은 고지방식이 급여시 감소되었으나 차전자수침액 투여에 의해 효과적인 회복이 있었다. 간손상 지표효소인 혈청 중의 AST, ALT 및 LDH 활성은 고지방식이를 급여할 경우 현저한 증가가 나타났으나 차전자수침액 병행 투여시 유의적인 감소를 나타내었다. 이러한 결과로 보아 차전자수침액의 함유 성분이 간기능을 증가시키며 지질대사에 영향을 미치므로써, 차전자가 고지혈증 및 간손상을 예방 또는 치료할 수 있을 것으로 사료된다. The present study has been undertaken to investigate the effect of plantaginis semen extract on lipid composition and activities of several enzymes in lard administrated rats. Forty male rats of Sprague-Dawley strain with average weight of 130±10g were divided into four groups [basal diet(BD), basal+plantaginis semen(BP), basal+lard(BL) and basal+plantaginis semen+lard (BLP)]. Lard administrated groups showed increase in weight gain and that of BLP group significantly increased. Feed intake showed no significant differences with lard administration. The weights of liver, kidney and heart of the rat were not affected by plantaginis semen and lard administration. Serum and liver triglyceride and cholesterol contents significantly increased compared to BD group but significantly decreased by the addition of plantaginis semen extract (BLP group). Serum and liver phospholipids decreased in lard administrated group but effectively restored by administration of plantaginis semen extract. HDL-cholesterol content significantly decreased in lard-fed group and somewhat increased in plantaginis semen extract group. Serum aminotransferase, lactate dehydrogenase activities significantly increased in lard fed group but addition of plantaginis semen extract significantly decreased the enzyme activities compared with that of lard administrated group.

Collarless 도재전장주조관의 제작 방법에 따른 치경부 변연적합도에 관한 연구

조수열,장기환,임시덕,주경삼 한국위생과학회 1997 한국위생과학회지 Vol.3 No.1

The purpose of this study was to evaluate the marginal gap in the collarless ceramo-metal restoration by the different techniques and materials. Three ceramic materials were used : 1) Vita porcelain 2) Ceramco porcelain 3) Vita porcelain and margin porcelain technique, wax technique, and LCS technique. The results were as follows : At point A, the marginal adaptation in case of Vita porcelain by LCS technique was best ㎛ and its marginal gap was 59.9 ㎛ At point B, the marginal adaptation was best in case of Vintage porcelain by margin porcelain technique and its marginal gap was 60.0 ㎛ at point B. At point C, the marginal adaptation was best in case of Vita porcelain by wax technique and its marginal gap was 60.1 ㎛.

길경 추출물의 간 보호효과

조수열,윤수홍,하헌,송휘수 한국위생과학회 1995 한국위생과학회지 Vol.1 No.1

Platycodi Radix extract was tested for therapeutic effects an the hepatic injuries models, environmental carcinogenic compound, benzo(a)pyrene induced liver damages. An increase in serum level of hepatic aminotransferase and alkaline phosphatase induced by i.p. treatment of benzo(a)pyrene was suppressed by pretreatment with extract at 0.15-0.5g/kg/day for 7 consecutive days. And lipid content was also changed especially by water ext. and chloroform ext. treatment. So it was concluded that Platycodi Radix could be a promising liver protective agent.

전통음료 소재가 납투여한 흰쥐의 체내 무기질 함량 및 간과 신장조직의 형태학적 변화에 미치는 영향

조수열(Soo-Yeul Cho),김명주(Myung-Joo Kim),이미경(Mi-Kyung Lee),박은미(Eun-Mi Park),장주연(Joo-Yeun Jang),최정목(Jeung Mok Choi),김덕진(Duk-Jin Kim) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.2

전통음료의 소재로 애용되는 녹차, 감잎, 홍화 및 두충의 열수추출물이 납투여된 흰쥐의 체내 무기질 함량 및 간과 신장조직의 형태학적 변화에 미치는 영향을 구명하기 위하여 체중 ㎏당 25 ㎎의 납을 매주 1회 경구투여하였다. 녹차잎, 감잎, 홍화씨 및 두충수피의 열수추출물은 매일 일정시간에 체중 ㎏당 1.26 g 수준이 되도록 4주간 경구투여하였다. 전통음료 소재의 열수추출물 급여시 납투여로 인한 혈청과 간조직의 납축적 억제와 혈청 중의 철과 구리 함량 증가에 효과적인 것을 관찰할 수 있었다. 납투여시 혈청 중 포도당과 알부민 함량은 유의적으로 감소되었으며 크레아티닌과 빌리루빈 함량은 유의적으로 증가되었다. 반면, 실험식이 급여시 이들 혈청 중 성분함량의 회복을 보였다. 또한 간조직 및 신장조직의 형태학적 변화는 납단독투여군에서 괴사성 소견 및 염증세포의 출현이 관찰되었으나 전통음료 소재의 열수추출물 급여시 조직학적 변화를 완화시키는 것으로 나타났다. This study was designed to investigate the effect of Korean traditional tea materials on lead (Pb) accumulation and histological changes in Pb-administered rats. Male rats were divided into six groups: a normal, Pb, and Pb-water extract of Korean traditional tea materials (green tea: GT, persimmon leaf: PL, safflower seed: SS, Eucommia ulmoides: EU) groups. Pb (25 ㎎/㎏ BW) was orally administered once a day for 4 weeks. The extract of GT, PL, SS, and EU were administered based on 1.26 g of raw traditional tea materials/㎏ BW/day. Pb contents of serum and liver were significantly higher in the Pb group than in the normal group. However, the water extracts of GT, PL, SS, and EU administration lowered Pb contents of serum and liver in the Pb-administered rats, respectively. Serum Zn content was significantly higher in the Pb group than in the normal group, whereas, Cu and Fe contents were lower in the Pb group. Hepatic Ca content was significantly lower in the Pb group compared to the normal group. In the present study, water extract of Korean traditional tea materials administration effectively improved Cu and Fe contents of serum and liver. Infiltration of the local inflammation was found in Pb group, whereas water extracts of Korean traditional tea materials administration attenuated histological changes of tissues.

천연자원의 간기능 보호 효과에 관한 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 울금의 효과 The Effect of Curcumae Rhizoma on Benzo(a)pyrene Induced Hepatotoxicity

조수열,윤수홍,이윤경,이정숙 효성여자대학교 부설 한국환경위생연구소 1992 환경위생연구 Vol.2 No.1

우리의 생활환경중 어디에나 존재하는 강력한 간독성유발물 benzo(a)pyrene으로 유도된 간장해에 대한 울금의 예방 및 치료효과를 관찰하여 다음과 같은 결과를 얻었다. 1. 울금 추출물 급여는 B(a)P투여로 증가한 혈청 ALT 와 ALP혈청을 현저히 회복시켰으나 혈청 AST활성은 회복시키지 못하였고, 혈청LDH의 활성은 전 실험군 사이에 유의적인 변화가 없었다. 2. 간조직 및 혈청중의 지질함량 변동은 B(a)P단독투여로 인한 유의성 있는 변화에 대해 단지 혈청중의 HDL-cholesterol과 total cholesterol 함량만이 울금 추출물 투여에 의해 정상범위로 회복되었다. 3. 간조직중의 glutathione 함량은 울금 추출물 투여로 B(a)P 단독투여군에 비해 유의성 있는 회복을 보였고, 과산화지질 함량은 B(a)P 단독투여로 인해 유의성 있게 증가하였으나 울금추출물 급여가 이런 증가를 회복시키지는 못했다. This study was performed to investigate the effect of Curcumae Rhizoma extract on the hepatic detoxication when benzo(a)pyrene, a hepatotoxi-cant, was administrated. The results were as followed : 1. The markedly increased enzyme activities(ALT, ALP) were decreasedby administration the Curcumae Rhizoma extract. 2. Serum HDL-cholesterol and total cholesterol contents were normal inthe Curcumae Rhizoma extract groups. 3. Liver glutathione content were decreased by administration the CurcumaeRhizoma extract. 4. It tended that the curative effects were better than the protective effectsof the Curcumae Rhizoma extract.

민들레잎추출물의 흰쥐 체내 지질대사 개선 효과

조수열(Soo-Yeul Cho),박지윤(Ji-Yoon Park),오연진(Yeon-Jin Oh),장주연(Joo-Yeun Jang),박은미(Eun-Mi Park),김명주(Myung-Joo Kim),김광수(Kwang-Soo Kim) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.4

흰쥐에게 1% 콜레스테롤과 0.25% 콜산나트륨을 첨가한 고콜레스테롤혈증 유발식이와 민들레추출물을 분획별(열수추출물군, 에틸아세테이트추출물군, 에테르추출물군)로 나누어 급여하여 민들레추출물이 고콜레스테롤 혈증에 미치는 영향을 구명하였다. 체중증가량과 식이섭취량은 대조군에 비하여 열수추출물군에서 유의적으로 증가되었으며 식이효율의 유의적인 차이는 관찰되지 않았다. 혈청과 간조직 중의 중성지질, 총콜레스테롤과 콜레스테릴에스테르 농도는 대조군에 비하여 민들레추출물 급여시 유의적으로 감소되었으며, 특히 에틸아세테이 트추출물군의 간조직 중의 중성지질, 유리콜레스테롤 및 총콜레스테롤의 감소효과가 현저한 것으로 나타났다. 반면, 혈청 인지질 농도는 민들레추출물 급여시 유의적인증가를 나타내었으며 간조직에서는 유의적이지는 않으나 증가되는 경향이었다.<br/> 혈청 중 킬로미크론, VLDL-콜레스테롤 농도는 민들레추출물 급여시 유의적으로 감소되었는데 열수추출물과 에틸아세테이트추출물군의 감소정도가 큰 것으로 관찰되었다. 반면, HDL-콜레스테롤 농도는 열수추출물과 에틸아세테이트추출물 급여시 유의적인 증가를 나타내었다. 동맥경화지수는 민들레추출물군 모두 유의적으로 감소되었다.<br/> 간조직 중의 HMG-CoA reductase 활성은 대조군에 비하여 민들레 열수추출물군에서 유의적으로 감소되었으며, ACAT 활성은 민들레추출물 급여군 모두 유의적인 감소를 나타내었다. 본 실험결과 민들레추출물 특히, 열수추출물과 에틸아세테이트추출물 급여는 고콜레스테롤식이를 급여한 흰쥐의 간조직 중 ACAT 활성을 감소시키므로써 혈청과 간조직의 총콜레스테롤과 유리콜레스테롤 축적을 억제하는 것으로 사료된다. The effect of dandelion leaf extracts on lipid metabolism was investigated in high cholesterol-fed rats. Four groups of rats were given a high cholesterol diet containing 10 g cholesterol/kg and 2.5 g sodium cholate/kg for 6 weeks. The control group received without dandelion leaf extracts and the other three groups received with one of dandelion leaf extracts (water, ethyl acetate, ether), respectively. Weight gain and feed intake were significantly higher in water group than control group. Serum triglyceride, total cholesterol, cholesteryl ester, chylomicron and VLDL-cholesterol concentrations were lower, whereas HDL-cholesterol and phospholipid concentrations were significantly higher in dandelion leaf extract supplemented-groups than control group. Dandelion extract increased HDL-C/total-C ratio and lowered atherogenic index. Hepatic triglyceride, total cholesterol, free cholesterol and cholesteryl ester concentrations lowered in dandelion leaf extract groups compared to control group. Especially, hepatic triglyceride and free cholesterol levels were lower in ethyl acetate extract group than in water and ether extract groups. Dandelion leaf extract supplements did not affect hepatic phospholipid cencentration. Hepatic HMG-CoA reductase activity was significantly lower in water extract group than the others. ACAT activity was significantly decreased by dandelion extract supplements.

콜레스테롤 첨가식이로 사육한 백서의 체내 무기질 및 간장조직 변화에 미치는 구리와 아연의 영향

조수열,정재홍,김덕진,임경혜 嶺南大學校 環境問題硏究所 1987 環境硏究 Vol.6 No.2

This experiment was undertaken to investigate the effect of copper and zinc on mineral status and liver histologic change in rats fed with cholesterol supplemented diets. Thirty-six male Sprague-Dawley rats, weighing 100±10g were employed in the study. Zinc was supplemented at 0ppm, 30ppm, 300ppm, and copper was 1.5ppm, 6ppm of the diet for 4weeks, respectively. Levels of Ca, Mg, Na, K, Cu, Zn, and Fe in liver showed no signigicant differences with dietary copper and zinc levels. Difference of copper level in kidney between high zinc group III (CNH), V(CLH), and normal zinc group I (cnn) was not found. The copper concentrations of the serum were lower in copper deficiency groups. In parenchymal cell of liver, group II(CNN) showed vacuolation and bile duct proliferation. Group III(CNH) & group IV(CNL) showed partly inflamnatory and necrosis. Group V(CLH) and VI(CLL) showed dilation of sinusoid and inflammatory cell infiltrated. And the level of lipid deposition was marked in group III(CNH) and mild in the rest of e rest of the groups.