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육철,백운화,박관화,Yook, Cheol,Pek, Un-Hua,Park, Kwan-Hwa 한국식품과학회 1991 한국식품과학회지 Vol.23 No.2
옥수수 전분을 propylene oxide와 반응시켜 치환도가 $0.031{\sim}0.147$되는 hydroxypropyl 옥수수 전분을 제조하여 물리화학적 특성을 규명하였다. Hydroxypropylation에 의하여 팽윤력, 용해도 및 물결합 능력이 증가하고 생전분의 효소 분해력이 높아졌으며 요오드 흡착강도가 낮아졌다. 아울러 hydroxypropylation에 의하여 전분의 결정화도도 감소되었다. 한편, 옥수수 전분을 hydroxypropylation시켰을 때 분자량 $1.34{\times}10^7$ 이상의 큰 분자는 감소하였고 $1.34{\times}10^7{\sim}1.18{\times}10^5$의 전분 분자는 증가하였다. Hydroxypropylation에 의하여 옥수수 전분 입자의 형태에는 별 변화가 없었으나 입자크기는 평균입경이 $13.9\;{\mu}m$에서 $16.0\;{\mu}m$로 증가하였다. Hydroxypropylated starches were prepared by reaction of corn starch with propylene oxide and their physicochemical properties were compared with those of the native starch. Swelling power, solubility and water binding capacity increased with the increase of hydroxypropylation. The hydroxypropylation of corn starch significantly reduced the extent of digestion and iodine absorption. Starch molecules larger than $1.34{\times}10^7{\sim}$ decreased whereas molecules ranging from $1.34{\times}10^7{\sim}1.18{\times}10^5$ increased by hydroxypropylation. Granule size increased by hydroxypropylation but this did not significantly affect the granule surface appearance by SEM. The hydroxypropylation improved the solubility and water binding capacity of corn starch.
고초균으로부터 β - glucanase 유전자의 분리 및 대장균에서의 발현
서연수,이영호,백운화 ( Yeon Soo Seo,Young Ho Lee,Un Hua Pek ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.4
A gene coding for β-glucanase which can specifically hydorlyze barley β-glucan and lichenan was isolated from Bacillus subtilis ATCC6633 by molecular cloning using a plasmid vector pBR325. 2.1 Kb BamHI-HindIII DNA fragment conferred β-glucanase activity on Escherichia coli HB101 and 1.7 Kb PstI DNA fragment included in 2.1 Kb fragment gave rise to β-glucanase producing colonies, while its expression depended upon its inserting orientation in pUC9. This gene directed efficiently the synthesis of β-glucanase in E. coli. Up to 2496 of total activity was found outside of the cells. Fine restriction map was also constructed for further sequencing analysis. The transcriptional direction of cloned β-glucanase gene was determined and its putative functional unit could be deducted from the molecular weight of β-glucanase and by deletion mapping.
김의식,손광수,지해성,명남진,백운화 ( Eui Sik Kim,Kwang Soo Son,Hae Sung Jee,Nam Jin Myung,Un Hua Pek ) 한국하천호수학회 1996 생태와 환경 Vol.29 No.1
This study was proposed to control algal biomass in order to improve transparency of an artificial pond, using the manipulation of a pond ecosystem. Zooplanktons isolated from natural populations were tested for their algal clearance rate. The size of two ponds were 1,500 and 2,000 m^3, respectively. Average depth of two ponds was shallow around 1 m. Fish were completely excluded from Pond-A instead of introducing zooplanktons, and Pond-B was added fish with the same condition of Pond-A. Turbidity of Pond-A was less than 10 FTU and transparency was about 1 meter depth during the experimental periods(May-Aug., 1995). In contrast, Chl-a and turbidity of Pond-B were 40 FTU and 55㎍·1^-1 and transparency was mostly less than 20 cm during summer season.
고핵산 함유 Saccharomyces cerevisiae 균주를 이용한 정미성 효모 추출물의 제조
김재식,김진욱,심원,김정완,박관화,백운화,Kim, Jae-Sik,Kim, Jin-Wook,Shim, Won,Kim, Jung-Wan,Park, Kwan-Hwa,Pek, Un-Hua 한국식품과학회 1999 한국식품과학회지 Vol.31 No.2
핵산 함량이 높은 변이주 Saccharomyces cerevisiae B24를 산업적으로 활용하기 위해 배양 후 자기소화 혹은 효소 분해법으로 효모 추출물을 제조하였다. 배양액의 균체 농도를 10% (w/w)로 하고 pH 5.0, $50^{\circ}C$에서 서서히 교반하면서 48시간 자기소화시켰을 때 세포 내용물이 효모 추출물로 이전되는 수율은 65% 정도였으나 리보핵산은 정미력이 없는 다른 성분으로 분해되어 정미성 핵산성분인 5'-IMP나 5'-GMP가 거의 검출되지 않았다. 반면 $90^{\circ}C$ 이상의 온도로 B24 배양액 1 L를 열처리하여 핵산 분해 효소를 불활성화시키고 세포벽을 변성시킨 다음 ${\beta}-1,3-glucanase$, phosphodiesterase, adenylic deaminase, protease 등을 순차적으로 작용시켜 정미성 5'-IMP와 5'-GMP가 총 3.2% 함유된 효모 추출물(고형분 함량 70%) 84 g을 얻을 수 있었고 이 때 추출물 수득율은 고형분 기준으로 85% 이었다. 반면 모균주인 S. cerevisiae ATCC 7754를 효소적으로 분해할 때 정미성 5'-IMP와 5'-GMP가 총 2.2% 함유된 효모 추출물(고형분 함량 70%)을 77 g밖에 얻지 못하였다. Yeast extracts were prepared using either autolysis or enzymatic digestion methods for industrial application of the Saccharomyces cerevisiae B24 strain developed previously to have high RNA content. Extraction ratio of yeast extract from yeast cell reached 65% when autolysis of yeast slurry having 10% solid content was induced at $50^{\circ}C$ and pH 5.0 by agitating with 100 rpm. However, neither 5'-IMP nor 5'-GMP was detected from the autolyzate. In another attempt to prepare a yeast extract S. cerevisiae B24 culture was treated at $90^{\circ}C$ and then treated by various enzymes including ${\beta}-1,3-glucanase$, phosphodiesterase (nuclease P1), adenylic deaminase, and a protease. The yeast extract prepared by the enzymatic digestion method contained 3.2g of 5'-IMP and 5'-GMP/100g dry yeast extract.