연속 2 단계 교반조 효소 반응장치의 최적화에 관한 연구

박영훈,유두영 한국화학공학회 1978 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.16 No.4

For production of 6-aminopenicillanic acid by enzymatic hydrolysis of benzylpenicillin, two-stage continuous stirred tank enzyme reactor system was evaluated. Using proper mathematical model of the reaction kinetics and kinetic constants of soluble and immobilized forms of penicillin amidohydrolase, the reactor system was simulated with an aid of a computer and a set of optimum operating conditions was found. In comparison with the single stage system, the productivity of the two-stage enzyme reactor system was 50% higher than that of the single stage system under the same operating conditions. For the industrial applications, the reactor system using immobilized penicillin amidohydrolase was found to be far more advantageous in terms of productivity than that using soluble enzyme because of the reduced inhibitory effect of the product, 6-aminopenicillanic acid, on the immobilized enzyme as compared to the same effect on the soluble enzyme.

영지식 증명을 이용한 가입기간이 정해진 서비스에서 이동 통신 기기간 인증 기법

박영훈,서승우,Park, Young-Hoon,Seo, Seung-Woo 대한전자공학회 2013 전자공학회논문지 Vol.49 No.11

이동 통신 기술이 발달함에 따라 통신 기기간의 메시지 교환이 가능한 서비스가 생겨나고, 그 사용 횟수가 폭발적으로 늘고 있다. 기기간의 통신을 위해서는 통신 기기간의 서비스 구성원이라는 인증이 선행되어야 한다. 하지만, 기존 인증 기술은 Trusted party와 같은 제 삼자와의 통신이 수반되는데, 이로 인하여 대역폭이 낭비되거나, 기지국 범위 밖의 이동 통신 기기는 기기간 통신에 참여할 수 없다는 문제점이 발생할 수 있다. 본 논문에서는 제 삼자의 개입이 없는 새로운 이동 통신 기기간 인증 기법을 소개할 것이다. 제안된 기술에 대하여, 서비스의 가입 여부 및 서비스 가입 시간을 모두 검증해야 하므로, 이를 가능하게 하는 새로운 영지식 증명 기법을 개발하여 적용할 것이다. 또한, 이 영지식 증명 기법은 인증정보를 암호화된 그대로 검증하기 때문에 증명 하고자 하는 기기의 프라이버시가 보장이 되며, 질의-응답 방식을 사용하기 때문에 다른 기기의 인증 메시지를 재사용하는 공격으로부터 보호할 수 있다. As the mobile communication technology is developed, the services for communication between the mobile devices are provided, and the amount of usage is increasing tremendously. For the device-to-device communication, the device should be verified if it is a service member. The existing verification schemes include interactions with the third party, while this may cause the problems that the bandwidth is dissipated and the devices which are out of the communication range of the base station cannot communicate with other devices. To solve such problems, we propose a new scheme for verification between mobile devices without interaction of third party. For the proposed scheme, we develop and employ a new zero-knowledge proof protocol, which verifies the device's membership and its expiration time. Furthermore, the scheme guarantees privacy of the mobile device since it checks the encrypted verification message without decrypting, and protects replaying attack since it uses challenge-response method.

생분해성 고분자소재 생산기술

박영훈 한국미생물학회 1993 微生物과 産業 Vol.19 No.1

국내에서의 생분해성고분자소재 생산에 관한 기술개발은 아직도 초기실험실적 개발단계로 평가되고 있다. 산업계에서는 태평양화학(주) 및 고려합성 등에서 연구개발 보고가 이루어진 바 있는데 고려합섬의 경우 미생물을 이용한 발효법으로 PHB생산 기술을 확보 현재 산업화를 위한 scale-up 연구를 진행시키고 있다고 하나 자세한 기술내용은 알려져 있지 않다. 현재 과기원, 서울대, 경북대 등에서 PHB 생산관련 연구가 활발히 진행되고 있으며 실험실적 결과도 매우 양호한 것으로 알려지고 있다.

LiBr와 CaBr₂가 흡착된 알루미나상에서 일어나는 염화 알킬의 브롬화반응

박영훈,신영문,조범준,김창배,Park, Yeong Hoon,Shin, Young Mun,Cho, Beom Jun,Kim, Chang Bae 한국공업화학회 1996 공업화학 Vol.7 No.5

알루미나에 LiBr와 $CaBr_2$를 각각 흡착시켜 용매가 없는 상태에서 여러 가지 염화 알킬과 반응시켜 좋은 수득률로 해당하는 브롬화 생성물을 얻을 수 있었다. 이러한 높은 반응성은 알루미나에 흡착되어 있는 극소량의 물과 브롬화 금속간의 상호작용이 알루미나 상에서 일어날 수 있기 때문이라는 것을 Differential scanning calorimetry (DSC) 열분석 실험과 문헌을 통하여 고찰하였다. Several alkyl chlorides were brominated using LiBr and $CaBr_2$ adsorbed on alumina in the absence of solvent. The yields of alkyl bromides were fairly high. Such a high reactivity by these bromide salts might be due to the interaction of minute amount of water and the salt on alumina. Differential scanning calorimetry (DSC) thermogram was provided and discussed.

가토에서 트리암시놀론이 증식유리체망막병증과 간세포 성장인자의 농도에 미치는 영향

박영훈,이원기,김경락,김현승,이영춘,정성근,Young Hoon Park,Won Ki Lee,Kyung Rak Kim,Hyun Seung Kim,Young Chun Lee,Sung Kun Chung 대한안과학회 2006 대한안과학회지 Vol.47 No.2

Purpose: To investigate the intravitreal level change of hepatocyte growth factor (HGF) in a rabbit model of proliferative vitreoretinopathy (PVR) and the effect of triamcinolone on PVR and intravitreal HGF concentration. Methods: Experimental PVR was induced in 21 New Zealand white rabbits by performing a scleral laceration, followed by repair and intravitreal injection of 0.1 ml of autologous blood. Seven eyes (group A) were injected with a high -dose of triamcinolone (5 mg), 7 eyes (group B) with a low -dose of triamcinolone (1 mg), and 7 control eyes (group C) with only balanced salts solution (BSS), respectively. Intravitreal HGF levels were measured by ELISA test taken at 0, 3, 7, 14, 21, and 28 days after PVR induction. PVR grade was determined by serial indirect ophthalmoscopic examination at different time points mentioned. Results: Elevated HGF levels were found in the vitreous peaking at 7 days after PVR -inducton in all 3 groups. The differences in HGF levels of treated groups A and B, and the control group C were statistically significant (p<0.001). The differences of PVR grades among the 3 groups were not statistically significant. However, gross and microscopic examinations of the control group C showed that proliferations and morphologic changes of retinal pigment epithelial cells were more prominent than in the treated groups. Conclusions: The intravitreal level of HGF varied with time in the rabbit PVR model. Also, our results suggest that intravitreal triamcinolone suppresses the expression of intravitreal HGF in the rabbit PVR model.

하중분배 계수를 적용한 비충전 강합성 바닥판 활하중 모멘트 산정

박영훈,김성훈,Park, Young hoon,Kim, Sung Hoon 대한토목학회 2014 대한토목학회논문집 Vol.34 No.6

강합성 바닥판은 두 방향의 휨 강성이 다르기 때문에 직교이방성의 성질을 가진다. 강합성 바닥판의 휨 강성비는 활하중 모멘트에 영향을 미친다. 비충전 강합성 바닥판의 휨 강성비는 메인 베아링 바 간격과 직접적인 관계를 가지고 있어 메인 베아링 바 간격과 형상비 영향이 고려된 하중분배 계수식에 대한 연구가 필요한 것으로 판단된다. 본 연구에서는 휨 강성비를 고려하는 AASHTO LRFD Bridge Design Specification에 의한 비충전 강합성 바닥판의 활하중 모멘트를 평가하고 비충전 강합성 바닥판의 하중분배 계수식을 제안하고자 한다. Because of the different flexural rigidity between longitudinal and transverse direction, orthotropic plate theory may be suitable for describing the behavior of composite deck. The ratio of flexural rigidity between longitudinal and transverse direction affects the live load moment. Because of the ratio of flexural rigidity of concrete unfilled steel grid deck has a direct relationship with main bearing bar spacing, it is concluded that the study for the distribution factor which is effected by main bearing bar spacing and aspect ratio is needed. In this study, evaluate the live load moment of concrete unfilled steel grid deck using the AASHTO LRFD Bridge Design Specification and presents the distribution coefficient equation for concrete unfilled steel grid deck.

정방형내에서 경사냉각면이 동결현상에 미치는 영향에 관한 연구

박영훈,김병철 조선대학교 에너지.자원신기술연구소 1998 에너지·자원신기술연구소 논문지 Vol.20 No.1

It was studied on the phenomena of freezing with inclination cooling surfaces in the cube One side of the test section was cooled and the other sides were insulated The effects for the initial temperature, the inclination angle on the temperature field and the shape of the ice-water interface were observed In the beginning of freezing, with increasing the inclination angle the freezing rate was increased and in the stable density layers the convective fluid flow became small, the freezing was fast In the region above the 4℃ of initial temperature, the frozen thickness in the inclined surface of the upper part was thinner than that of lower part, but with time the frozen thickness of upper part was thicker than that of lower part, and also below the 4℃ of initial temperature the frozen thickness in the upper part was thicker than that of lower part from the beginning. 기호설명 H Height of enclosure GREEKS L length of enclosure η Dimensionless vertical coordinate [y/H] T Temperature ε Dimensionless horizontal coordinate [x/L] x Horizontal coordination SUBSCRIPTS y Vertical coordination i inital t Time c cold θ Angle of cooling surface

Phenazin 環을 가진 界面活性劑의 合成과 그 抗菌性(제2보)

朴永勳 尙志大學校 1983 論文集 Vol.4 No.-

7-Alkyl-1-hydroxyphenazine derivatives, bearing butyl or octyl group, were synthesized by the condensation reaction of pyrogallol-1-monoethylether with 3,4-diaminoalkylbenzene, prepared from aniline and alcohols as starting materials through alkylation of 3-nitro-4-aminoalkylbenzene. And the products were also identifed by elementary analysis, IR and NMR spectra. Ir spectra were determined in pressed potassium bromide disks. Nmr spectra were determined on a Varian spectrometer and shifts are reported in parts per million with TMS as an internal standard. Surface tension of these derivatives in aqueous solution was determined. And also antibiotic activity of these derivatives was tested, using Gram positive and negative bacillus and fungi. It showed that the antibiotic activity of these alkyl-substituted 1-hydroxyphenazine derivatives was stronger than that of the 1-hydroxyphenazine.

다양한 탐구활동을 통한 학습자의 학습태도와 흥미 신장

박영훈 한국미래교육학회 2015 미래교육연구 Vol.5 No.2

학생중심의 다양한 탐구활동 수업을 통해 학습자의 학습 태도와 흥미 신장의 변화를 알아보기 위한 본 연구는 문제풀이에만 치중하는 학생들에게 과정을 중요시하게하고 탐구의 즐거움을 느낄 수 있도록 하며, 수학 교과학습에 대한 학생들의 흥미 신장과 자기주도적 학습 태도를 배양할 수 있는 교수·학습 방법을 고안·실천하였다. 실천과제로는 첫째, 2009개정 수학과 교육과정과 교과서의 기본학습내용 분석을 통해, 탐구활동 수업을 위한 준비를 하였으며, 둘째, 중1수학 교과의 단원별 탐구활동 계획을 세우고 자료를 수집 재구성하여 다양한 탐구활동 수업 방법을 구안하였고, 셋째, 재구성한 자료를 교수·학습 활동에 적용하여 학생들의 수학교과에 대한 학습태도와 흥미의 변화를 알아보았다. 이러한 활동 결과 학생들의 수학교과에 대한 태도 변화에서 긍정적으로 대답한 비율이 향상되었으며 이는 수업에 적극적으로 참여하고 학습의욕을 키우며 스스로 학습하는 태도에도 영향을 미치는 것으로 나타났다. 또한, 수학 교과에 대한 긍정적 태도 변화는 수학에 대한 흥미 신장으로 연결되었다고 볼 수 있다. This study examines the instructional methods of math exploration activities and their influence on students’ math knowledge, interest, and self-directed learning skills. In particular, the study focuses on the improvement of students’ learning attitude as they do not learn through math drills but through math exploration activities that put emphasis on the process of solving math problems. In order to do this, the 2009 National Curriculum and textbook content were analyzed, looking for ways to prepare for the exploration activities. Then, various exploration activities were created through the planning as well as reconstructing instructional materials. Third, reconstructed materials were carried out through instructional methods in order to see the changes in students’ learning attitudes and interests in mathematics. After the activities, the result showed that students had a positive change in their math learning attitude. It appeared that students’ motivation and active participation had increased students’ desire to learn, and affect their self-directed learning skills. In addition, positive changes in students’ learning attitude seemed to be connected with their improved interest in mathematics.

Pyrolysis characteristics and Kinetic parameters of jatropha curcas

박영훈,김동현,김진수,김승수 한국공업화학회 2007 한국공업화학회 연구논문 초록집 Vol.2007 No.1