일산화탄소가 뇌내 신경전달물질 및 그 합성효소에 미치는 영향

윤재순 梨花女子大學校 藥學硏究所 1991 藥學硏究論文集 Vol.- No.1

We studied the effect of carbon monoxide(CO)-induced bypoxia on monoamine neurotransmitter and their synthetic enzyme in rat brain. When the rats were acute or chronic intoxicated at CO 5000 ppm for 30 minutes or 2000ppm for 1 week each 3hours, dopamine content increased significantly with decreasing of its turnover on striatum and norepinephrine content was diminished in hypothalamus. 5-hydroxytryptamine content was increased significantly and its turnover was decreased both in striatum and hypothalamus. Tryosine hydroxylase activity was reduced in striatum. These results suggest that inhibition of TH activity in CO-induced hypoxia is owing to lack of oxygen supply threfore NE content is decreased. We suggest that increasing of dopamine and 5-hydroxytryptamine are due to reduction of its turnover.

內分泌先調疾患에 대한 高麗人蔘의 藥效에 대한 硏究 : 甲狀腺疾患에 대하여 In Hypo or Hyperthyroidism

尹再順 梨花女子大學校 韓國生活科學硏究院 1980 韓國生活科學硏究院 論叢 Vol.26 No.-

호르몬으로 인한 甲狀腺機能亢進症과 低下症의 血淸中 酸素活性, 脂質, 蛋白質, 糖量 등을 측정하였을 때 亢進症에서는 S-GOT, S-GPT의 活性度 上昇, 總 cholesterol, trigyceride, 總脂質量 低下, 蛋白質量 低下, 血糖量 上昇과 같은 病的 狀態가 나타났으며, 여기에 人參을 투여하면, S-GOT, S-GPT의 活性度 上昇은 억제되고 血糖量은 低下되는 藥效를 나타내었고, 其他에 대해서는 통계학적으로 有意性 있는 영향을 미치지 못하였다. 低下症에서는 S-GOT, S-GPT, S-LDH 活性上昇, 總 cholesterol. 總脂質量 上昇, trigly-ceride 量 低下, 蛋白質量 上昇, 血糖量 低下와 같은 病的 狀態를 역시 招來했으나 人參 투텨로 酵素活性은 低下되고 總 cholesterol, 總脂質量의 증가는 억제되었고, 血糖量도 증가되어 正商化하는 데 有效하였다. 즉 TH 內分泌失調症患인 亢進症과 低下症의 血淸 중 成分變動은 人參으로 正商化되는 데 有效하였으며, 有害作用은 없었다. Activities of S-GOT, S-GPT, and S-LDH in rat serum those have been in hypo and huper-thyridism were investigated under the influences of ginseng extract in order ot find the thera-perutic effect. The levels of total lipids, proteins and glucose were measured concerning with the same aims. Activites od S-GOT, S-GPT, and levels of the serum glucose were proved to increase in hyperthyroidism, however levels of total cholesterol, triglyceride and total lipids were reduced. Raising of S-GOT, S-GPT activities and glucose level were stabilized to normal level by treatment of ginseng extract. In hypothyroidsm, activities of S-GOT, S-GPT, and S-LDH were found to increase, levels of total cholesterol, total lipies and proteins in serum increased altogether. But reductuon was observed in the levels of triglyceride and glucose in the same serum. Adding the ginseng extract, acthivities of the above three enzymes were stabilized, and cholesterol and lipids were normalized in their levels. Variation of these levels caused by either hypo or hyperthyroidism were found to be stabi-lizabie by the addition of ginseng extract giving no side effects.

Aloe vera가 Alloxan 糖尿흰쥐의 血糖量 및 Insulin量에 미치는 영향에 관한 硏究

尹再順,金惠善 梨花女子大學校 韓國生活科學硏究院 1983 韓國生活科學硏究院 論叢 Vol.31 No.-

Aloe vera Linne가 組織再生作用과 細胞復活作用이 있으며 췌장 β-cell의 insulin分泌上皮細胞의 增植過程을 촉진한다는 報告가 있어 A. vera의 糖尿病에 對한 效果와 그 作用機轉을 규명하고 저 alloxan 糖尿흰쥐와 epinephrine에 의한 一時的 過血糖흰쥐에게 A. vera, chloropropamide 또 두 藥物을 倂用投與하여 血糖量과 glycogen量 및 血中 insulin量의 變化를 Somogyi-Nelson法과 Radioimmunoassay法으로 各各 測定하였다. A. vera는 alloxan糖尿흰쥐에 있어서 血糖量降下作用과 肝藏中 glycogen含量增加作用이 있었으며 epinephrine投與에 의한 一時的過血糖흰쥐에 對해서 A. vera前處置로 血糖量上昇作用이 抑制되었다. 또한 alloxan 糖尿흰쥐에 對해서 A. vera는 insulin分泌促進作用으로 血中 insulin量이 增加되었다. They reported that Aloe vera was effective in regeneration of wound tissues and cells, and that Aloe vera stimulated the proliferative process in regeneration of insular epithelium of the pancreas. This experiment was carried out to investigate the effect of Aole vera on Diabetes mellitus. Crud powder of Aloe vera and chloropropamide were given orally to alloxan diabetic rats either alone or in combination. Level of blood glucose and serum insulin in alloxan diabeic rats were measured by Somogyi-Nelson method and radioimmunoassay method respectively. We also investigated that effect of Aloe vera on blood glucose level in transient hyperglycemic rats by epinephrine. Thus, it is possible to conclude that Aloe vera had lowering effect on blood glucose level and increasing effect on glycogen content in alloxan diabetic rats, inhibiting effect on induction of transient hyperglycemia by epinephrine, and stimulaing effect on the recretion of insulin in alloxan diabetic rats.

Eicosanoid 유도체가 흰쥐 혈압 변화에 미치는 영향

윤재순,윤연숙,신정희,최현진,최진아 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

Arachidonic acid (AA, C20:4, w-6) and eicosapentanoic acid (EPA,C20:5, w-3), which are polyunsaturated fatty acids forming eicosanoids, were tested for their effects on blood pressure in Wistar rats and SHR. AA is the most important precursor for the biosynthesis of eicosanoids which include the prostaglandins, prostacyclin (PGI_2), thromboxane A_2 (TXA_2) and the leukotriens. TXA_2 is a potent vasoconstrictor and a powerful inducer of platelet aggregation causing myocardial infarction and hypertention. In contrast, PGI_2 induces vasodilation and inhibits platelet aggregation. In this study, AA markedly increased blood pressure, but its effect was antagonized by both EPA a structural analog of AA, and dazmegrel, a TX synthetase inhibitor. Also, AA enhanced the antihypertensive effects of hydralazine and captopril, and EPA reduced TXA_2 production. These results indicate that the hypotensive effects of EPA might be closely related to the decrease in TXA_2 biosynthesis due to competitive inhibition by structural similarity of the EPA to the AA, the precursor of TXA_2.

뇌 저산소성 장해시의 뇌내 신경전달물질 및 에너지대사 물질의 변화

윤재순 대한약학회 1990 大韓藥學會 總會 및 學術大會 Vol.39 No.-

일산화탄소 중독 저산소증 마우스의 대뇌피질 신경세포 분포에 관한 연구

尹再順,安有淑,李熙來 梨花女子大學校 韓國生活科學硏究院 1991 韓國生活科學硏究院 論叢 Vol.47 No.-

We studied the changes of the distribution on nerve cells in cerebral cortex of hypoxic mice induced by carbon monoxide(CO). When the mice exposed to 5,000 ppm CO for 60 minutes, carboxyhemoglobin level was 82%. At zero, 1st, 3rd, 5th, 7th and 14th day after CO intoxication, hypoxic and normal mice were fixed by 3.5% formalin and each of motor(area 4), somatosensory(area 3) and visual(area 17) cortices of the cerebral cortex, was sectioned and stained with cresyl violet. Using light microscope quantitative analysis was followed. Our results could be summarized as follows : The insignificant and significant decreases in number of nerve cells, were observed in the motor and somatosensory cortices, at zero, 1st day and at 3rd, 5th, 7th, 14th day after CO intoxication respectively. The same mode of decreases in the number of nerve cells was also observed in the visual cortex, at zero and at 1st, 3rd, 5th, 7th, 14th day after CO intoxication respectively. A series of decreasing phenomena in the above results were more evident in visual cortex than motor and somatosensory cortices. It may be concluded from these findings that the decrease of nerve cells in the mice exposed to CO causes the damage of nerve cell in the cerebral cortex and it is connected with necrosis.

Flunarizine이 腦虛血性흰쥐의 腦에너지 代謝에 미치는 영향

尹再順,徐銀珠 梨花女子大學校 韓國生活科學硏究院 1989 韓國生活科學硏究院 論叢 Vol.44 No.-

뇌허혈증은 뇌순환장해성질환이며 Ca^2+의 과량 유입으로 조직세포의 막전압이 변하여 정상적에너지산생이 어려워지며 결국 뇌세포는 괴사에 이르게 된다. 양측총경동맥을 결찰하여 뇌허혈을 일으킨후 대뇌피질내 에너지대사관련물질을 정량한 결과 ATP, Pcr, 및 glucose량이 정상뇌에 비해 감소하였고 lactate량은 증가하였다. 이러한 현상은 정상쥐에 비해 노령화쥐, 선천성고혈압쥐, DOCA-salt 고혈압쥐 순으로 뚜렷하였다. 허혈성흰쥐에 Ca^2+길항제인 flunarizine을 2배의 등비수열적용량 10, 20, 40mg/kg을 복강내에 전처치 하였을 때 뇌에너지대사장해는 용량의존적으로 회복되었다. 이때도 노령화쥐가 회복율이 가장 부진하였다. 허혈증모델에 flunarizine 전처치한 정상혈압허혈증모델이나 경동맥결찰 30분후 뇌혈류를 재순환시킨 어린쥐는 뇌에너지대사관련물질이 거의 정상뇌에 가깝게 회복되었다. 이와같은 결과로 flunarizine은 세포내 Ca^2+의 과잉유입을 방지하여 적혈구변형능저하방지, 혈관수축억제작용으로 뇌혈류를 증진시켜 뇌에너지대사를 개선하여 뇌보호작용이 나타난 것이라 생각된다. Recent hypothesis suggests that intracellular accumulation of Ca^2+ is a common denominator of ischemic cellular damage. Flunarizine, Ca^2+ entry blocker, increases cerebral blood flow in cerebral vascular diseases. The present study was designed to evaluate the effect of flunarizine on cerebral energy metabolism in the ischemic rat brain made by bilateral carotid artery ligation. The content of ATP, creatine phosphate and glucose decreased while lactate increased after thirty minutes ligation in the aging rats, the spontaneous hypertensive rats and the DOCA-salt treated hypertensive rats. Flunarizine improved changes of the cerebral energy metabolites in a dose-dependent-response pattern. Especially, resuscitation of brain ischmic damage showed a remarkable recovery of energy metabolite to the normal state of the brain. The results showed that flunarizine ameliorated the cerebral energy metabolism in the ischemic rat brain and had a brain protective effect.

중추성 항고혈압약이 뇌내 신경전달물질의 생합성 효소에 미치는 영향

윤재순 대한약학회 1985 약학회지 Vol.29 No.4

It has been reported that clonidine is $\alpha_2$-adrenergic agonist, potnet new hypotensive drug in human with low dose. The change in blood pressure is implicated in the concentration, release, uptake and metabalism of catecholamine and activity of catecholamine synthesizing enzyme in specific brain areas. Thus the experiment was set up to investigate the effect on the enzyme activity of clonidine alone and that of clonidine pretreated with imipramine or tranylcypromine by measuring activity of the Dopa-forming enzyme, tyrosine hydroxylase (TH) and epinephrine forming enzyme, phenylethanolamine-N-methyl transferase (PNMT) in brain and adrenal gland. The TH activity in brainstem and substantia nigra is decreased by intraperitoneally administered clonidine 0.1mg/kg twice a day for 5 days, but increased in the rats pretreated with imipramine 10mg/kg intraperitoneally given 26 hrs and 5 hrs before decaptitation. However the TH activity in all regions of brain is increased in rats pretreated with tranylcypromine 10mg/kg intraperitoneally twice a day for 5 days. The effect of clonidine on TH activity is due to inhibition release of norepinephrine by activation of presynaptic $\alpha_2$-adrenoreceptor, axon terminal result in the decrease of TH activity in brain. The increasing of TH activity in brain results in attenuation of the role of clonidine by pretreated with imipramine or tranylcypromine in rats. The activity of PNMT was not significantly affected by clonidine, imipramine and tranylcypromine in adrenal gland.

Clonidine의 혈압강하및 적출정관 평활근수축에 미치는 6-Hydroxydopamine의 영향

윤재순,장문희 대한약학회 1987 약학회지 Vol.31 No.2

The effect of neurotoxic compound 6-hydroxydopamine (6-OHDA) on the change in blood pressure and contractile response of Vas deference by centrally acting agents has been studied in normal and DOCA-salt induced hypertensive rats. The treatment of neonatal rats with 6-OHDA (2$\times$100mg, 250mg Kg$^{-1}$s.c) significantly inhibited the antihypertensive and relaxant effects of Vas deference of clonidine(100$\mu\textrm{g}$ Kg$^{-1}$iv.). The simultaneous administration of desipramine with clonidine into neonatal rats decreased the antihypertensive response of clonidine although treated did not affect the relaxative response of Vas deference. Furthermore, the antihypertensive and relaxant responses of clonidine were reduced by the neonatal rats with 6-OHDA regardless of the administration of desipramine. When neonatal rats were administered with 6-OHDA, the development of DOCA-salt hypertension was prevented. These results suggest that 6-OHDA, clonidine and desipramine hada significant effect on the development and the inhibition of central hypertension mediating the central adrenergic neuron due to their affinity to the central nervous system.

국내의 돼지 인플루엔자 바이러스(H1N1, H3N2)의 혈청학적 조사

윤재순,박봉균,한정희,Yoon, Jai-soon,Park, Bong-kyun,Han, Jeong-hee 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.3

Swine influenza is an acute, infectious respiratory disease caused by type A influenza viruses in pigs. In the previous studies, serological surveys have indicated the presence of H3N2 swine influenza virus (SIV) since 1995 in Korea. And the percentage of the antibody-positive rate was 39.12% in the survey determining the prevalence of H1N1 SIV antibodies in 2002. The purpose of this study was therefore to investigate the sero-prevalence of SIV regard to the age of the pig and the season between June 2004 and May 2005. In this study, a total of 932 sera were used. These sera were randomly selected from blood samples, which were submitted to Department of Veterinary Pathology, Kangwon National University and Department of Veterinary Virology, Seoul National University from June 2004 to May 2005. These sera have been tested by ELISA test kit (IDEXX Lab, USA) for the SIV H3N2, H1N1 respectively. SAS version 9.1 was used for the statistical analysis based on the age of the pig and the season. The overall sero-prevalence of the antibody against H3N2 SIV was 20.82% (194/932). The overall sero-prevalence of the antibody against H1N1 SIV was 37.23% (347/932). The overall dual sero-prevalence of the antibody against H3N2 and H1N1 SIV was 10.62% (99/932). H3N2 has significant difference in statistically regarding the age of the pig and the season (p<0.0001). H1N1 has significant difference in statistically regarding the age of the pig (p<0.0001) but has not significant difference in statistically regarding the season (p=0.5882).