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감광성고분자의 합성에 관한 연구(II) -Aromatic Azide에 의한 Polyvinyl Alcohol의 광가교
오경남,홍성일 한국섬유공학회 1983 한국섬유공학회지 Vol.20 No.3
Photosensitive polymers of 4, 4-diazidodiphenyl+PVA, diazidoterephthalate + PVA, and PVA-p-azidobenzoate were prepared and their photosensitive characters were studied. The results showed that the gel fraction was 0.85 for PVA-p-azidobenzoate and 0.45 for 4, 4-diazidodiphenyl+PVA and diazidoterephalate +PVA respectively. Sensitizers of acetophenone, benzophenone and 5-nitroacenaphthene were added, and following effects were observed for each type of photosensitive resins. In the case of 4, 4-diazidodiphenyl+PVA, all sensitizers increased gel fraction to the same value of 0.55. However, in the case of diazidoterphthalate + PVA, gel fraction was increased to 0.70 by benzophenone and 0.55 by acetophenone. When benzophenone, acetophenone and 5-nitroacenaphthene were added to PVA-p azidobenzoate, the gel fractions were increased to be 0.95, 0.90 and 0.90, respectively.
방사선 조사가 분리대두단백의 기능적, 구조적 특성에 미치는 영향
오경남,이숙영 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.3
The effects of irradiation on the functional and structural characteristics of soy protein isolates were studied. Soymilk was irradiated at 1, 5, and 10kGy, after which soy protein isolates were prepared. The functional properties of soy protein isolates were examined including solubility, emulsion capacity and stability, foam capacity and stability, structural properties as represented by SDS-PAGE pattern, and secondary and tertiary structures. The solubility and emulsion capacity were increased by radiation treatment at 1kGy however the values were adversely affected again as dosage was increased above 5kGy. As irradiation dosage increased, an increase of foaming capacity at lkGy and a decreasing turnover afterwards were also noted in foaming capacity, although the differences were not statistically significant. The SDS-PAGE pattern showed fragmentation and aggregation of protein molecules as affected by irradiation in proportion to the dosage increase. The results of CD and fluorescence spectroscopy revealed increased aperiodic structure contents with the dosage increase. It was assumed that irradiation dosagefrom 5 to 10kGy could initiate minimal denaturation of protein in various foods compared to general heat treatment.
DNA Comet Assay를 이용한 콩류의 방사선 조사 확인
오경남(Kyeong-Nam Oh),김경은(Kyeung-Eun Kim),양재승(Jae-Seung Yang) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.5
콩류의 방사선 조사 여부를 DNA comet assay로 확인 하였다. 대두, 강남콩, 그리고 팥을 각각 0.1, 0.3, 0.5, 0.7, 그리고 1.0 kGy의 저선량으로 조사하고 비조사 시료와 조사시료간의 DNA 손상정도를 현미경으로 육안 검사 및 comet의 tail 길이를 측정하여 상호 비교하였다. 모든 시료에서 비조사 시료보다 조사시료의 tail 길이가 더 길었으며 조사 선량이 증가할수록 tail 길이가 길게 나타났다. 비조사 시료에서도 손상된 세포의 comet 모양의 핵이 관찰되기는 하였으나 대부분 비손상된 세포의 원형모양의 핵이 관찰되었으며 조사된 시료에서는 comet 모양의 핵이 주로 관찰되는 경향이어서 비조사 시료와 조사시료간에 comet 양상을 비교할 수 있었다. 한편 100개의 세포를 무작위로 선택하여 comet tail 길이를 측정하고 통계분한 결과, 비조사 시료와 조사시료간의 유의적인 차이가 있었고 조사선량이 증가할수록 tail 길이가 유의적으로 증가함을 확인하였다. DNA comet assay로 간단하고 비교적 저렴한 비용으로 짧은 시간 내에 콩류의 방사선 조사 여부를 확인할 수 있었다. The single cell-gel electrophoresis assay (comet assay) was used to identify irradiated beans. Soy beans, kidney beans, and red beans were irradiated with (60)^Co gamma rays at 0.1, 0.3, 0.5, 0.7, and 1.0 kGy. Beans were peeled out, crushed lightly, and treated with phosphate-buffered saline (PBS) to extract cells. The extracted cell suspension was mixed with agarose gel solution and spread on an agarose precoated slide. After lysis of the cells, they were subjected to microgel electrophoresis for 2 minutes, and then silver-stained. We found that the DNA fragments of the irradiated samples were stretched, migrated out of the cells, and formed tails towards the anode giving the appearance of comets, while the unirradiated or the undamaged cells formed very short or no tails. The tail lengths of irradiated samples were significantly increased as irradiation dose increased at the above 0.3 kGy.