Human amnion-derived mesenchymal stem cells induced osteogenesis and angiogenesis in human adipose-derived stem cells via ERK1/2 MAPK signaling pathway

( Yuli Wang ),( Xichen Chen ),( Ying Yin ),( Song Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.4

Mesenchymal stem cells (MSCs) have shown great potential in treating bone deficiency. Human adipose-derived stem cells (HASCs) are multipotent progenitor cells with multi-lineage differentiation potential. Human amnion-derived mesenchymal stem cells (HAMSCs) are capable of promoting osteogenic differentiation of MSCs. In this study, we investigated the effect of HAMSCs on HASCs by a transwell co-culture system. HAMSCs promoted proliferation, osteogenic differentiation, angiogenic potential and adiponectin (APN) secretion of HASCs. Moreover, the positive effect of HAMSCs was significantly inhibited by U0126, a highly selective inhibitor of extracellular signaling-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) signaling pathway. These observations suggested that HAMSCs induced bone regeneration in HASCs via ERK1/2 MAPK signaling pathway. [BMB Reports 2018; 51(4): 194-199]

A Comparison of Friction Force Calibration in Lateral Force Microscope

Wang Yuli-Ang,Kim Hong-Joon,Kong Ho-Sung,Zhao Xu-Zheng,Yoon Eui-Sung Korean Tribology Society 2006 KSTLE International Journal Vol.7 No.1

The main principle of two widely used methods which were proposed by Ruan and Bhushan, and by Ogletree and Carpick are introduced. Experiments were conducted using the two methods to measure friction force between AFM probe and silicon sample quantitatively. To characterize the frictional properties, the conversion factors of the two methods by which lateral electronic signal is converted into actual friction force were calculated. The experimental results show that that the conversion factors were extraordinarily different from each other. Further research should be done to identity the reasons for these differences.

Human Amnion-Derived Mesenchymal Stem Cells Protect Human Bone Marrow Mesenchymal Stem Cells against Oxidative Stress-Mediated Dysfunction via ERK1/2 MAPK Signaling

Wang, Yuli,Ma, Junchi,Du, Yifei,Miao, Jing,Chen, Ning Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.3

Epidemiological evidence suggests that bone is especially sensitive to oxidative stress, causing bone loss in the elderly. Previous studies indicated that human amnion-derived mesenchymal stem cells (HAMSCs), obtained from human amniotic membranes, exerted osteoprotective effects in vivo. However, the potential of HAMSCs as seed cells against oxidative stress-mediated dysfunction is unknown. In this study, we systemically investigated their antioxidative and osteogenic effects in vitro. Here, we demonstrated that HAMSCs significantly promoted the proliferation and osteoblastic differentiation of $H_2O_2$-induced human bone marrow mesenchymal stem cells (HBMSCs), and down-regulated the reactive oxygen species (ROS) level. Further, our results suggest that activation of the ERK1/2 MAPK signal transduction pathway is essential for both HAMSCs-mediated osteogenic and protective effects against oxidative stress-induced dysfunction in HBMSCs. U0126, a highly selective inhibitor of extracellular ERK1/2 MAPK signaling, significantly suppressed the antioxidative and osteogenic effects in HAMSCs. In conclusion, by modulating HBMSCs, HAMSCs show a strong potential in treating oxidative stress- mediated bone deficiency.

Human Amnion-Derived Mesenchymal Stem Cells Protect Human Bone Marrow Mesenchymal Stem Cells against Oxidative Stress-Mediated Dysfunction via ERK1/2 MAPK Signaling

Ning Chen,Yuli Wang,Junchi Ma,Yifei Du,Jing Miao 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.3

Epidemiological evidence suggests that bone is especial-ly sensitive to oxidative stress, causing bone loss in the elderly. Previous studies indicated that human amnion-derived mesenchymal stem cells (HAMSCs), obtained from human amniotic membranes, exerted osteoprotective effects in vivo. However, the potential of HAMSCs as seed cells against oxidative stress-mediated dysfunction is unknown. In this study, we systemically investigated their antioxidative and osteogenic effects in vitro. Here, we demonstrated that HAMSCs significantly promoted the proliferation and osteoblastic differentiation of H2O2-induced human bone marrow mesenchymal stem cells (HBMSCs), and down-regulated the reactive oxygen species (ROS) level. Further, our results suggest that activation of the ERK1/2 MAPK signal transduction pathway is essential for both HAMSCs-mediated osteogenic and protective effects against oxidative stress-induced dysfunction in HBMSCs. U0126, a highly selective inhibitor of extracellular ERK1/2 MAPK signaling, significantly suppressed the antioxidative and osteogenic effects in HAMSCs. In conclusion, by modulating HBMSCs, HAMSCs show a strong potential in treating oxidative stress- mediated bone deficiency.

A Novel In Vitro Human Colon Model System for Investigating Interaction of Gut Microbes with Human Cells

Raehyun KIM,Peter ATTAYEK,Yuli WANG,Kathleen FURTADO,Rita TAMAYO,Christopher SIMS,Nancy ALLBRITTON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Healthy human bodies host hundreds of trillion microbes. More than 99% of these microbes colonize the gut. These gut microbes, also called gut microbiota, have huge impacts on the health and disease states of the hosts. An in vitro intestinal model system that enables co-culturing the gut microbes with human cells can be a valuable tool to elucidate their interaction. However, creating a model system that possesses a proper oxygen gradient to meet different oxygen demands of the gut microbes, primarily obligate anaerobic bacteria, and the human cells, has been challenging. Here, we show a simple in vitro human colon model system that creates the physiological oxygen gradient by utilizing the cellular oxygen consumption and modulating oxygen influx. The behaviors of the primary human colon epithelial cells in the oxygen gradient were compared to those cultured in the conventional aerobic condition. Multiple obligate anaerobic gut bacterial strains were successfully co-cultured in the model system. This novel and simple in vitro colon model system may be a valuable tool to study the interaction of gut bacteria and the host cells.

Self-sustaining oxygen gradient formation and its use for investigating the gut bacteria/host interactions

김래현,( Pete Attayek ),( Yuli Wang ),( Kathleen L. Furtado ),( Rita Tamayo ),( Nancy L. Allbritton ) 한국공업화학회 2022 한국공업화학회 연구논문 초록집 Vol.2022 No.-

Hydration Behaviors of Portland Cement with Different Lithologic Stone Powders

Zuqiang Xiong,Peng Wang,Yuli Wang 한국콘크리트학회 2015 International Journal of Concrete Structures and M Vol.9 No.1

In this study, influence of different stone powders (SP), including limestone powders (LP), quartzite powders (QP), and granitic powders (GP), on the hydration behaviors of portland cement, for example, setting time, hydration heat, and hydration products, were discussed. The initial and the final setting time both shorten when the content of LP is 5 %, however, they are slightly delayed by the other two SPs. The LP has no obvious influence on the arrival time of the first peak in the exothermal curves, and it makes the peak value decrease; the other two SPs postpone the appearance of the first peak, and they also make the peak value decrease. For the second peak, LP shifts the peak position to the left, QP has no effect on this peak position, and GP makes the appearance of this peak delayed by 143 min. Similarly, three kinds of SPs have different influence on the hydration products of portland cement. The LP precipitates the formation of hydrated calcium carbo aluminate, the QP the formation of hydrated garnet, and the GP makes the amount of Tobermorite increase.

Increased Circulating CXCL10 in Non-Segmental Vitiligo Concomitant with Autoimmune Thyroid Disease and Alopecia Areata

( Li Zhang ),( Xinya Xu ),( Shujun Chen ),( Yuli Kang ),( Xiuxiu Wang ),( Chengfeng Zhang ),( Leihong Xiang ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.4

Background: Vitiligo is a common acquired pigmentary disease caused by destruction of epidermal melanocytes in underlying autoimmune response. Few studies have been focused on the role of chemokines in non-segmental vitiligo (NSV) concomitant with autoimmune thyroid disease (AITD) and alopecia areata (AA). Objective: The aim of this study was to determine the best serum biomarker for predictive role in the progression of vitiligo and to evaluate the influence of AA and/or AITD on vitiligo by using the biomarker. Methods: This prospective cohort study recruited 45 NSV patients: 14 without either AITD or AA, 12 with AITD, 11 with AA, and 8 with both AITD and AA. Serum levels of CXCL1, CXCL8, CXCL9, CXCL10, CXCL12, CXCL13, and CXCL16 were analyzed by ELISA. CXCR3 mRNA expression was detected on PBMCs by RT-PCR. Improvement was evaluated using repigmentation scales. Results: Serum CXCL10 levels, along with the expression of CXCR3 mRNA were higher in NSV patients with AITD or AA alone than in those without AITD or AA. Moreover, serum CXCL10 levels, along with the expression of CXCR3 mRNA were higher in NSV patients with both AITD and AA than in those with AITD or AA alone. Poorer repigmentation was observed in NSV patients with both AA and AITD than in those with AA or AITD alone. Conclusion: CXCL10 could be a biomarker to predict the progression of NSV. Dermatologists should pay much attention to those NSV patients concomitant with AITD and/or AA, for comorbidity might lead to more active autoimmune reaction. (Ann Dermatol 31(4) 393∼402, 2019)

An Ultra-narrow Bandwidth Filter for Daytime Wind Measurement of Direct Detection Rayleigh Lidar

Han, Fei,Liu, Hengjia,Sun, Dongsong,Han, Yuli,Zhou, Anran,Zhang, Nannan,Chu, Jiaqi,Zheng, Jun,Jiang, Shan,Wang, Yuanzu Optical Society of Korea 2020 Current Optics and Photonics Vol.4 No.1

A Rayleigh Lidar used for wind detection works by transmitting laser pulses to the atmosphere and receiving backscattering signals from molecules. Because of the weak backscattering signals, a lidar usually uses a high sensitivity photomultiplier as detector and photon counting technology for signal collection. The capturing of returned extremely weak backscattering signals requires the lidar to work on dark background with a long time accumulation to get high signal-to-noise ratio (SNR). Because of the strong solar background during the day, the SNR of lidar during daytime is much lower than that during nighttime, the altitude and accuracy of detection are also restricted greatly. Therefore this article describes an ultra-narrow bandwidth filter (UNBF) that has been developed on 354.7 nm wavelength of laser. The UNBF is used for suppressing the strong solar background that degrades the performance of Rayleigh wind lidar during daytime. The optical structure of UNBF consists of an interference filter (IF), a low resolution Fabry-Perot interferometer (FPI) and a high resolution FPI. The parameters of each optical component of the UNBF are presented in this article. The transmission curve of the aligned UNBF is measured with a tunable laser. Contrasting the result of with-UNBF and with-IF shows that the solar background received by a Licel transient recorder decreases by 50~100 times and that the SNR with-UNBF was improved by 3 times in the altitude range (35 km to 40 km) compared to with-IF at 10:26 to 10:38 on August 29, 2018. By the SNR comparison at four different times of one day, the ratio-values are larger than 1 over the altitude range (25~50 km) in general, the results illustrate that the SNR with-UNBF is better than that with-IF for Rayleigh Lidar during daytime and they demonstrate the effective improvements of solar background restriction of UNBF.

Effect of Magnesium Carbonate on Hydration and Hardened Properties of Portland Cement Paste

Fanghui Li,Zuqiang Xiong,Cheng Wang,Yuli Wang 대한토목학회 2020 KSCE Journal of Civil Engineering Vol.24 No.12

The effects of magnesium carbonate on the setting time and compressive strength of Portland cement paste were studied when the content of magnesium carbonate was 0wt%, 0.5wt%, 1wt%, 2wt%, and 3wt% by the weight of cement. The influence mechanism was analyzed through combining with the tests of hydration heat, X-ray diffraction (XRD), differential thermal analysis-thermogravimetric analysis (DTA-TG), and scanning electron microscope (SEM). The results show that magnesium carbonate makes the setting time of cement paste shorten, and makes the compressive strengths of cement paste increase at 1 day and 7 days, but decrease at 28 days. The testing results of the heat of hydration, XRD, SEM, and DTA-TG show that magnesium carbonate promotes the formation of Ettringite (AFt) and the hydration of calcium silicate in the early stage when its content is less than 1wt%, but the addition of magnesium carbonate is unfavorable to the later hydration of Portland cement. Based on the above analysis, it is suggested that the content of magnesium carbonate is not more than 1wt%, which has good reference value for using magnesium carbonate to prepare early strength agent for cement paste.