제주 수생식물에서 분리한 내생균류의 다양성

오유선(Yoosun Oh),문혜연(Hye Yeon Mun),고재덕(Jaeduk Goh),정남일(Namil Chung) 한국생명과학회 2017 생명과학회지 Vol.27 No.6

내생균류는 숙주식물 내에서 생물학적 그리고 비생물학적 스트레스로부터 저항성을 갖는데 도움을 준다. 수생식물은 수생환경에 서식하며 육상식물보다 수분스트레스에 더 많이 노출되어있다. 본 연구에서는 제주에 있는 남생이못, 수장동습지, 용수저수지, 강정천 4개의 습지에서 11개의 수생·수변식물을 채집하여 연구하였다. 전처리를 통해 식물 외생균을 제거하고 뿌리에서 내생균류를 분리하였다. 남생이못에서 126균주, 수장동습지에서 22균주, 용수저수지에서 44균주, 강정천에서 32균주가 분리되어 총 224개의 내생균류를 분리하였다. 분리된 균은 ITS를 이용해 동정한 후, 다양성 분석을 하였다. 남생이못에서 분리된 내생균류는 4개 문(Phylum), 7개 강(class), 12개 목(order), 19개 과(family), 30개 속(genus)로 구분되었다. 수장동습지에서 분리된 내생균류는 4개 문, 5개 강, 6개 목, 11개 과, 11개 속으로 구분되었으며, 용수저수지에서 분리된 내생균류는 4개 문, 5개 강, 7개 목, 12개 과, 13개 속으로 구분되었다. 강정천에서 분리된 내생균류는 1개 문, 2개 강, 5개 목, 7개 과, 9개 속으로 구분되었다. 4개의 습지에서 공통으로 분리된 속은 Alternaria속, Colletotrichum속, Fusarium속이었다. 향후 내생균류의 다양한 생태학적 분포와 다양성 규명에 대한 연구에 기초자료로 이용될 것이다. Endophytic fungi are present in host plants and contribute to resistance to biotic and abiotic stress. Aquatic plants are living in aquatic environment such as saltwater or freshwater and exposed more water stress than other land plants. In this study, we investigated 4 wetlands in Jeju and collected 11 aquatic plants. Exogenous microbes were removed by preprocessing of plants and endophytic fungal strains were isolated from the plants. We isolated 126 fungal strains from Namsaengi-pond, 22 fungal strains from Sujangdong-marsh, 44 fungal strains from Yongsu-reservoir and 32 fungal strains from Gangjeongcheon. The fungal strains were identified using internal transcribed spacer (ITS) region and analyzed the phylogeny and diversity. Endophytic fungi isolated from plants of Namsaengi-pond were classified to 30 genera, 19 families, 12 orders, 7 classes and 4 phyla. Endophytic fungi of Sujangdong-marsh were classified to 11 genera, 11 families, 6 orders, 5 classes and 4 phyla. Endophytic fungi of Yongsu-reservoir were classified to 13 genera, 12 families, 7 orders, 5 classes and 4 phyla. Endophytic fungi isolated from Gangjeongcheon were classified to 9 genera, 7 families, 5 orders, 2 classes and 1 phyla. Overall, they were divided 40 genera and Alternaria, Colletotrichum and Fusarium were isolated from 4 sites in common. By investigating the endophytic fungi in aquatic plants, it is for baseline data that determination of diversity and the ecological distribution of endophytic fungi.

제주지역 수생식물에서 발굴된 Sordariomycetes강 균류 3종의 국내 최초 보고

오유선 ( Yoosun Oh ),문혜연 ( Hye Yeon Mun ),고재덕 ( Jaeduk Goh ),정남일 ( Namil Chung ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.2

Three endophytic fungal strains were isolated from aquatic plants collected in Jeju. The fungi were identified by sequencing and phylogenetically analyzing their internal transcribed spacer (ITS) regions. Consequently, the three strains NNIBRFG2982, NNIBRFG2984 and NNIBRFG 3040 were confirmed as the fungal species Acremonium tubakii, Colletotrichum cliviae and Cylindrocarpon pauciseptatum, respectively, all of which are new to Korea.

정신과 입원환자의 신체적 문제에 대한 조사

문유선,임기영,김도훈,이혜리,유계준,오병훈,민경준 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.6

This study was designed to determine the frequency and characteristics of physical symptoms and diseases in the psychiatric inpatients, and was directed to assess the physical problems and possible ways to resolve them in a closed psychiatric unit. Through evaluation of medical records of 386 inpatients from Kwangju Severance Psychiatric Hospital of Yonsei Medical College during a one-year peroid, from September 1, 1993 to August 31, 1994, we classified the physical problem according to ICD-9CM, comparing with the contents of primary health care in care in family practice. The results were as follows ; Among the total number of 386 psychiatric inpatients, 159 patients(41.2%) had the physical problems with 196 physical diagnoses(1.23 diagnoses per a patient). The most common diagnoses were constipation, upper respiratory tract infection, tinea, alcholic liver disease, dental caries in a descending order. The most common systemic diagnoses were diseases of digestive system, skin, ENT(ear, nose and throat)and dental diseases. The 81.6% of the physical problesm were consulted to a family physician with referral rate of 5.3%. Our results suggest that the majority of the physicial problems in a closed psychiatric unit could be resolved by consulting to a family physician.

아나운서의 직업 만족도와 직업 지속의도

황유선(Yoosun Hwang),오미영(Meeyoung Oh) 한국콘텐츠학회 2013 한국콘텐츠학회논문지 Vol.13 No.6

본 연구의 목적은 방송 콘텐츠 제작 종사자로서 아나운서의 직업 현실을 파악하려는 것이다. 이를 위해 아나운서들의 직업 만족도와 지속 의도를 직업 선택 동기, 조직 정체성, 그리고 사회적 평판 차원에서 살피고자 하였다. 아나운서들의 실제 직업 만족도와 직업 지속 의도에 가장 영향을 미치는 요인이 무엇이며, 아나운서 직종이 과연 대중이 인식하는 이른바 인기 직종 관념과 얼마나 부합하는지 밝히기 위한 것이다. 연구방법은 현직 아나운서 대상 설문조사 방식을 사용하였다. 응답자는 총 106명이었다. 위계적 회귀분석 결과 아나운서들의 직업 만족도와 지속 의도는 업무에 대한 외재적 동기가 아닌, 내재적 동기와 관련이 큰 것으로 밝혀졌다. 아나운서 업무 만족도 및 직업 지속 의도와 가장 밀접한 관계를 지닌 요인은 조직 일치감으로 나타났으며, 사회적 평판 요인은 직업 만족도와 지속 의도에 모두 유의미한 영향력을 발휘하지 못하는 것으로 드러났다. 이는 아나운서의 직업 만족도와 지속 의도가 외적 보상이나 조건 및 평가에 크게 좌우되지 않는다는 것을 의미한다. The object of this study is to determine and prioritize the factors that affect job satisfaction and job continuity intention of an important media content provider group - TV/radio announcers. In this study, the determinant factors are classified as “internal” factors (i.e. career motivation and identity as a member of an organization) and “external” factors such as reputation and prestige. A survey was conducted among active announcers in Korea, and 106 responded. The data was processed by multiple regression analysis. The result showed that organizational identity affected the job satisfaction and job continuity intention most, and perceived external prestige affected least. In conclusion, announcer’s job satisfaction and job continuity intention have more meaningful relationship with internal factors than with external factors.

First Reports of Unrecorded Mortierellomycetes and Umbelopsidomycetes Fungi from Freshwater Ecosystems in Korea

Jaeduk Goh,Yoosun Oh,Hye Yeon Mun 한국균학회 2023 韓國菌學會誌 Vol.51 No.3

In this study, we isolated several fungal strains from filtered water and sediment collectedfrom rivers and streams. The strains were identified by molecular phylogenetic analyses ofrDNA sequences (internal transcribed spacer [ITS], large subunit of ribosomal DNA [LSU]). The morphological characteristics of the fungi were investigated using microscopy, and theculture characteristics of fungi grown on several media were examined. We identified fourspecies previously unknown in South Korea, namely, Dissophora globulifera, Linnemanniaexigua, Mortierella rishikesha and Umbelopsis autotrophica.

Association of Change in Smoking Status and Subsequent Weight Change with Risk of Nonalcoholic Fatty Liver Disease

Jeong Seogsong,Oh Yun Hwan,Choi Seulggie,Chang Jooyoung,Kim Sung Min,Park Sun Jae,Cho Yoosun,Son Joung Sik,Lee Gyeongsil,Park Sang Min 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.1

Background/Aims: Smoking is considered a risk factor for the development of nonalcoholic fatty liver disease (NAFLD). However, the association of a weight change after a change in smoking status and the risk of NAFLD remains undetermined. Methods: This study used the Korean National Health Insurance Service-National Sample Cohort. Based on the first (2009 to 2010) and second (2011 to 2012) health examination periods, 139,180 adults aged at least 40 years were divided into nonsmoking, smoking cessation, smoking relapse, and sustained smoking groups. NAFLD was operationally defined using the fatty liver index. The adjusted odds ratio (aOR) and 95% confidence interval (CI) were calculated using multivariable-adjusted logistic regression. Results: Compared to nonsmoking with no body mass index (BMI) change, the risk of NAFLD was significantly increased among subjects with BMI gain and nonsmoking (aOR, 4.07; 95% CI, 3.77 to 4.39), smoking cessation (aOR, 5.52; 95% CI, 4.12 to 7.40), smoking relapse (aOR, 7.51; 95% CI, 4.81 to 11.72), and sustained smoking (aOR, 6.65; 95% CI, 5.33 to 8.29), whereas the risk of NAFLD was reduced among participants with BMI loss in all smoking status groups. In addition, smoking cessation (aOR, 1.76; 95% CI, 1.35 to 2.29) and sustained smoking (aOR, 1.64; 95% CI, 1.39 to 1.94) were associated with higher risk of NAFLD among participants with no BMI change. The liver enzyme levels were higher among participants with smoking cessation and BMI gain. Conclusions: Monitoring and management of weight change after a change in smoking status may be a promising approach to reducing NAFLD.

Isolation and Characterization of Previously Undescribed Seventeen Fungal Species Belonging to the order Hypocreales in Korea

Goh Jaeduk,Oh Yoosun,Park Young-Hwan,Mun Hye Yeon,Park Sangkyu,Cheon Wonsu 한국균학회 2022 韓國菌學會誌 Vol.50 No.1

Hypocreales is one of the largest orders within the class Sordariomycetes in Ascomycota. Several species of this order are cosmopolitan and have a broad range of habitats. Here, we isolated several fungal strains from environmental samples, including freshwater sediment and plant litter. The strains were identified via molecular and phylogenetic analyses of rDNA and other DNA markers, such as TUB , RPB2 , and EF1 . The morphological characteristics of the fungi were investigated using microscopy, and culture characteristics were assessed from their growth on several media. We identified 17 species previously unrecorded in Korea: Dactylonectria hordeicola, Flavocillium bifurcatum, Fusarium luffae, Ilyonectria ilicicola, Ilyonectria qitaiheensis, Ilyonectria robusta, Lecanicillium aphanocladii, Nectria ulmicola, Neonectria lugdunensis, Ovicillium oosporum, Pseudonectria foliicola, Sarocladium spinificis, Scolecofusarium ciliatum, Trichoderma appalachiense, Trichoderma subviride, Trichoderma taiwanense, and Trichoderma tsugarense. Hypocreales is one of the largest orders within the class Sordariomycetes in Ascomycota. Several species of this order are cosmopolitan and have a broad range of habitats. Here, we isolated several fungal strains from environmental samples, including freshwater sediment and plant litter. The strains were identified via molecular and phylogenetic analyses of rDNA and other DNA markers, such as , , and . The morphological characteristics of the fungi were investigated using microscopy, and culture characteristics were assessed from their growth on several media. We identified 17 species previously unrecorded in Korea: Dactylonectria hordeicola, Flavocillium bifurcatum, Fusarium luffae, Ilyonectria ilicicola, Ilyonectria qitaiheensis, Ilyonectria robusta, Lecanicillium aphanocladii, Nectria ulmicola, Neonectria lugdunensis, Ovicillium oosporum, Pseudonectria foliicola, Sarocladium spinificis, Scolecofusarium ciliatum, Trichoderma appalachiense, Trichoderma subviride, Trichoderma taiwanense, and Trichoderma tsugarense.

Seven Previously Unrecorded Fungal Species Isolated from Freshwater Ecosystems in Korea

( Jaeduk Goh ),( Hye Yeon Mun ),( Yoosun Oh ) 한국균학회 2021 韓國菌學會誌 Vol.49 No.2

Various freshwater ecosystems, such as streams, lakes and rivers, provide a dynamic habitat for fungi. In this study, we isolated several fungal strains from freshwater sediment and plant litter. The strains were identified using molecular phylogenetic analyses of rDNA and beta tubulin (TUB) sequences. Morphological characteristics of the fungi were also investigated using microscopy and culture characteristics of the fungi grown on several media. We identified seven species previously unrecorded in Korea, Nothophoma spiraeae, Westerdykella aquatica, W. aurantiaca, W. dispersa, Chrysosporium sanyaense, C.pseudomerdarium and Taeniolella phialosperma.

Four Species of Montagnulaceae Unrecorded in Korea and Isolated from Plant Litter in Freshwater

( Jaeduk Goh ),( Hye Yeon Mun ),( Yoosun Oh ),( Namil Chung ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4

Freshwater is a diverse and complex environment for fungi and provides several types of habitat including plant litter, sediment, and carcasses. We collected plant litter from the main stream and branch streams of Nakdong River, Sohancheon in Samcheok, and Geumoreum in Jeju. From several samples of plant litter, we isolated 8 fungal strains belonging to 4 Montagnulaceae species unrecorded in Korea: Paraconiothyrium archidendri, Paraphaeosphaeria sporulosa, Paraphaeosphaeria michotii, and Paraphaeosphaeria viridescens. These fungi were identified by phylogenetic analysis using the internal transcribed spacer (ITS) region and examination of morphological characteristics. Montagnulaceae is known as one of the families in Pleosporales and includes coniothyrium-like fungi. In this study, we described phylogenetic analysis and mycological characteristics of these species, and this is the first report of these taxa in Korea.

강원 삼척 및 경북 영주의 담수지역에서 발굴된 수생균류 3종의 국내 최초 보고

문혜연 ( Hye Yeon Mun ),고재덕 ( Jaeduk Goh ),오유선 ( Yoosun Oh ),정남일 ( Namil Chung ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4

Three aquatic fungi were isolated from samples of freshwater-deposited plant litter and foam collected in Samcheok, Gangwon-do, and Yeongju, Gyeongsangbuk-do, Korea. Based on their morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS) rDNA region, the three isolates NNIBRFG329, NNIBRFG339, and NNIBRFG19 were confirmed as aquatic fungi: Articulospora tetracladia, Margaritispora aquatica, and Aquanectria penicillioides. These species were known as aquatic fungi but neither species has been previously reported in Korea.