제주지역 수생식물에서 발굴된 Sordariomycetes강 균류 3종의 국내 최초 보고

오유선 ( Yoosun Oh ),문혜연 ( Hye Yeon Mun ),고재덕 ( Jaeduk Goh ),정남일 ( Namil Chung ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.2

Three endophytic fungal strains were isolated from aquatic plants collected in Jeju. The fungi were identified by sequencing and phylogenetically analyzing their internal transcribed spacer (ITS) regions. Consequently, the three strains NNIBRFG2982, NNIBRFG2984 and NNIBRFG 3040 were confirmed as the fungal species Acremonium tubakii, Colletotrichum cliviae and Cylindrocarpon pauciseptatum, respectively, all of which are new to Korea.

제주 수생식물에서 분리한 내생균류의 다양성

오유선(Yoosun Oh),문혜연(Hye Yeon Mun),고재덕(Jaeduk Goh),정남일(Namil Chung) 한국생명과학회 2017 생명과학회지 Vol.27 No.6

내생균류는 숙주식물 내에서 생물학적 그리고 비생물학적 스트레스로부터 저항성을 갖는데 도움을 준다. 수생식물은 수생환경에 서식하며 육상식물보다 수분스트레스에 더 많이 노출되어있다. 본 연구에서는 제주에 있는 남생이못, 수장동습지, 용수저수지, 강정천 4개의 습지에서 11개의 수생·수변식물을 채집하여 연구하였다. 전처리를 통해 식물 외생균을 제거하고 뿌리에서 내생균류를 분리하였다. 남생이못에서 126균주, 수장동습지에서 22균주, 용수저수지에서 44균주, 강정천에서 32균주가 분리되어 총 224개의 내생균류를 분리하였다. 분리된 균은 ITS를 이용해 동정한 후, 다양성 분석을 하였다. 남생이못에서 분리된 내생균류는 4개 문(Phylum), 7개 강(class), 12개 목(order), 19개 과(family), 30개 속(genus)로 구분되었다. 수장동습지에서 분리된 내생균류는 4개 문, 5개 강, 6개 목, 11개 과, 11개 속으로 구분되었으며, 용수저수지에서 분리된 내생균류는 4개 문, 5개 강, 7개 목, 12개 과, 13개 속으로 구분되었다. 강정천에서 분리된 내생균류는 1개 문, 2개 강, 5개 목, 7개 과, 9개 속으로 구분되었다. 4개의 습지에서 공통으로 분리된 속은 Alternaria속, Colletotrichum속, Fusarium속이었다. 향후 내생균류의 다양한 생태학적 분포와 다양성 규명에 대한 연구에 기초자료로 이용될 것이다. Endophytic fungi are present in host plants and contribute to resistance to biotic and abiotic stress. Aquatic plants are living in aquatic environment such as saltwater or freshwater and exposed more water stress than other land plants. In this study, we investigated 4 wetlands in Jeju and collected 11 aquatic plants. Exogenous microbes were removed by preprocessing of plants and endophytic fungal strains were isolated from the plants. We isolated 126 fungal strains from Namsaengi-pond, 22 fungal strains from Sujangdong-marsh, 44 fungal strains from Yongsu-reservoir and 32 fungal strains from Gangjeongcheon. The fungal strains were identified using internal transcribed spacer (ITS) region and analyzed the phylogeny and diversity. Endophytic fungi isolated from plants of Namsaengi-pond were classified to 30 genera, 19 families, 12 orders, 7 classes and 4 phyla. Endophytic fungi of Sujangdong-marsh were classified to 11 genera, 11 families, 6 orders, 5 classes and 4 phyla. Endophytic fungi of Yongsu-reservoir were classified to 13 genera, 12 families, 7 orders, 5 classes and 4 phyla. Endophytic fungi isolated from Gangjeongcheon were classified to 9 genera, 7 families, 5 orders, 2 classes and 1 phyla. Overall, they were divided 40 genera and Alternaria, Colletotrichum and Fusarium were isolated from 4 sites in common. By investigating the endophytic fungi in aquatic plants, it is for baseline data that determination of diversity and the ecological distribution of endophytic fungi.

담수환경에서 발굴된 Didymella 속 3종의 국내 최초 보고

문혜연 ( Hye Yeon Mun ),고재덕 ( Jaeduk Goh ),오유선 ( Yoosun Oh ),정애란 ( Ae-ran Jeong ),정남일 ( Namil Chung ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.1

Three fungal isolates, NNIBRFG108, 1139, and 1480, were isolated from freshwater environments; NNIBRFG108 from plant litter in Samcheok, Gangwon and NNIBRFG1139 and 1480 from the soil in Jeju & Gimcheon, Gyeongbuk, Korea. Based on the morphological characteristics and phylogenetic analysis of internal tanscribed spacer (ITS), 28S rDNA region, and β-tubulin gene, NNIBRFG108, NNIBRFG1139, and NNIBRFG1480 isolates were confirmed as Didymella segeticola, D. ellipsoidea, and D. aeria, respectively. Neither species has previously been described in Korea.

강원 삼척 및 경북 영주의 담수지역에서 발굴된 수생균류 3종의 국내 최초 보고

문혜연 ( Hye Yeon Mun ),고재덕 ( Jaeduk Goh ),오유선 ( Yoosun Oh ),정남일 ( Namil Chung ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4

Three aquatic fungi were isolated from samples of freshwater-deposited plant litter and foam collected in Samcheok, Gangwon-do, and Yeongju, Gyeongsangbuk-do, Korea. Based on their morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS) rDNA region, the three isolates NNIBRFG329, NNIBRFG339, and NNIBRFG19 were confirmed as aquatic fungi: Articulospora tetracladia, Margaritispora aquatica, and Aquanectria penicillioides. These species were known as aquatic fungi but neither species has been previously reported in Korea.

무제치늪에 자생하는 식물의 뿌리에서 분리한 내생진균의 군집분석 및 다양성 분석

천우재(Woo-Jae Cheon),최혜림(Hye-Rim Choi),김현(Hyun Kim),남윤종(Yoon-Jong Nam),오유선(Yoosun Oh),정민지(Minji Jeong),이난영(Nan-Yeong Lee),하상철(Sang-Chul Ha),김종국(Jong-Guk Kim) 한국생명과학회 2016 생명과학회지 Vol.26 No.12

이 논문은 무제치늪에서 채집한 기장대풀(Isachne globosa Kuntze), 솔방울고랭이(Scirpus karuisawensis Makino), 이삭귀개(Utricularia racemosa Wall.), 좀개수염(Eriocaulon decemflorum Maxim.)등 4종의 식물 뿌리에서 서식하는 내생곰팡이의 분포 및 다양성을 확인하기 위해 조사하였다. 총 226 균주가 분리되었고, 3문(Phyla), 7강(Class), 10목(Order), 22과(Family), 31(genera)속으로 분리되었다. 내생진균에서 가장 많이 분리된 속은 Acephala (19.9%), Tolypocladium (16.3%), Neopestalotiopsis (11.5%), Perenniporia (7.1%) 순으로 확인하였다. 다양성지수 분석에 있어서, 이삭귀개(Ur)가 다른 식물 종에 비해 종 풍부도 (Menhinick’s index = 2.37), (Margalef’s index = 4.46)와, 종 균등도 (Simpson’s index diversity = 0.91), 그리고 종 다양성(Shannon’s indx = 2.57)로 높게 나왔다. 이러한 분석 결과를 봤을 때, 이삭귀개(Ur)에서 내생진균이 가장 다양하게 서식할 수 있는 것으로 보여진다. Wetlands exhibit intermediate characteristics of both terrestrial and aquatic ecosystems, and the bio-diversity is rich in these unique biological habitats. The symbiotic relationships between plants and fungi that inhabit these wetlands play an important role in natural resource management, biodiversity, and conservation. Accordingly, the mujechi, having academic value for the study of the natural environment, was investigated in terms of genetic diversity of endophytic fungi, which inhabit the roots of wild plants. The internal transcribed spacer (ITS) region was amplified to identify fungal strains. In total, 226 strains were isolated and categorized into three phyla, seven classes, 10 orders, 22 families, and 31 genera. In plants by endophytic fungi were classified in Isachne globosa (Ig) to 19 genera, Scirpus karuisawensis (Sk) to 11 genera, Utricularia racemosa (Ur) to 19 genera, and one incertae sedis, Eriocaulon decemflorum (Ed) to 11 genera. The fungal taxa was identified the genera Acephala (19.9%), Tolypocladium (16.3%), Neopestalotiopsis (11.5%), and Perenniporia (7.1%). The fungal group isolated from Isachne globosa (Ig) grew the largest number of isolated fungal strains. After comprehensive evaluation, the endophytic fungal group from Utricularia racemosa (Ur) ranked highest in diversity analyses. From the roots of wild plant in mujechi-neup, it confirmed the distribution and diversity of endophytic fungi. This study provides the basic data to understand fungal community structure in peat wetlands.

정신과 입원환자의 신체적 문제에 대한 조사

문유선,임기영,김도훈,이혜리,유계준,오병훈,민경준 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.6

This study was designed to determine the frequency and characteristics of physical symptoms and diseases in the psychiatric inpatients, and was directed to assess the physical problems and possible ways to resolve them in a closed psychiatric unit. Through evaluation of medical records of 386 inpatients from Kwangju Severance Psychiatric Hospital of Yonsei Medical College during a one-year peroid, from September 1, 1993 to August 31, 1994, we classified the physical problem according to ICD-9CM, comparing with the contents of primary health care in care in family practice. The results were as follows ; Among the total number of 386 psychiatric inpatients, 159 patients(41.2%) had the physical problems with 196 physical diagnoses(1.23 diagnoses per a patient). The most common diagnoses were constipation, upper respiratory tract infection, tinea, alcholic liver disease, dental caries in a descending order. The most common systemic diagnoses were diseases of digestive system, skin, ENT(ear, nose and throat)and dental diseases. The 81.6% of the physical problesm were consulted to a family physician with referral rate of 5.3%. Our results suggest that the majority of the physicial problems in a closed psychiatric unit could be resolved by consulting to a family physician.