Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

Reinforcing Techniques and Property Evaluations of Electromagnetic Shielding Effective Fabrics Based on Polypropylene-coated Carbon Fibers

Jan-Yi Lin,Mei-Chen Lin,Yan-Yu Lin,Ting An Lin,Chen-Hung Huang,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3

In this study, the coating process is employed on metal wire to help improve the friction resistant property ofcarbon fibers, thereby provides more application feasibility. The yarn coating technique for reinforcement and woven fabricprocess are used to produce carbon/stainless steel/polyester/polypropylene/acrylic (CSPPA) woven fabrics that arecharacterized with softness and a light weight. The constituent coated yarns exhibit good conductivity after being coated witha PP layer, and likewise strengthen the woven fabrics in terms of mechanical behavior of tensile strength, elongation, bendingtorsion, creep resistance, and wear-resistant properties. The test results indicate that in the woven process, samples retaingood morphology. Due to PP sheath, the tensile strength of woven fabrics increases from 23 MPa to 42 MPa. Although thelamination layer numbers does not improve the EMI SE of woven fabrics, the EMI SE still reaches over 40 dB. Themanufacturing design proposed in this study provides an innovative finishing for carbon fibers without affecting the intrinsicproperties, and provides a greater range of application for carbon fibers.

The Field Test Result of Silicate Coating Using on Wood

( Ching Yi Lin ),( Naoto Ando ) 한국목재공학회 2011 한국목재공학회 학술발표논문집 Vol.2011 No.-

Performance Improvement with a Combined Scheme of Rapid Thermal Annealing and Multi-channel Structure for Poly-Si TFTs with Various Device Dimensions

Ching-Lin Fan,Yi-Yan Lin,Yan-Hang Yang 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.8

This study presents a combined scheme of rapid thermal annealing (RTA) and multi-channel structure for improving the performance of polycrystalline silicon (poly-Si) thin film transistors (TFTs). Results show that RTA treatment can improve device performance by decreasing the number of in-grain defects and improving the quality of the gate oxide. The multi-channel structure improves the electrical characteristics of the poly-Si TFTs by decreasing the number of grainboundary defects. Thus, the combined scheme can effectively reduce defect states and improve the gate-oxide’s quality simultaneously, resulting in a significant improvement in the performance of poly-Si TFTs. In addition, the performance improvement is particularly apparent for the TFTs with a thinner gate oxide and smaller channel length. Therefore, the proposed scheme is a good candidate for improving the electrical characteristics of TFTs for use in small-to-medium size displays.

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( Lin Yi-ching ) 한국기초조형학회 2018 한국기초조형학회 작품집 Vol.2018 No.1

Controlled Release of Clenbuterol from a Hydroxyapatite Carrier for the Treatment of Alzheimer’s Disease

Yi-Wen Lin,Chih-Hsiang Fang,Ya-Jyun Liang,Ching-Yun Yang,Wei-Ting Kuo,Feng-Huei Lin 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Alzheimer’s disease is a neurodegenerative disorder, and Aβ aggregation is considered to be the central process implicated in its pathogenesis. Current treatments are faced by challenges such as serious side effects and reduced drug bioavailability. In this study, we developed a drug delivery system for intramuscular injection that uses cellular activity to achieve constant and long-term drug release. Methods Synthesized mesoporous hydroxyapatite (SHAP) was prepared via co-precipitation, and hydrophobic surface modification using stearic acid was then used to load clenbuterol by physical absorption, thus creating the drug delivery system. Clenbuterol release was achieved through cellular activity, with macrophage uptake triggering lysosome/endosome disruption, cytoplasmic release, extracellular exocytosis, and subsequent systemic circulation. Results We found that clenbuterol-loaded SHAP enabled sustained release for more than 2 weeks and effectively modulated inflammation, reduced Aβ oligomer-induced toxicity, and prevented Aβ aggregation. Conclusions Our findings suggest that treatment with clenbuterol loaded in this SHAP delivery system could be a promising strategy for treating Alzheimer’s disease.

Shielding-benefit Evaluation of Electromagnetic Radiation and UV Radiation for Multifunctional Composite Polypropylene Woven Fabrics

Ting An Lin,Yu-Chun Chuang,Jan-Yi Lin,Mei-Chen Lin,Ching-Wen Lou,Keng Siang Sim,Jia-Horng Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.10

People have increasingly rising health consciousness in recent years and researchers are thus devoted themselvesto develop multi-functional textile products. In this study, stainless steel (SS) filaments are used for electromagnetic shieldingeffectiveness (EMSE) while polypropylene (PP) filaments are used for ultraviolet resistance and good mechanical properties. Spinning and weaving continuous formation techniques are employed to produce wrapped yarns with SS and PP filaments,after which a weaving process is employed for the preparation of SS/PP woven fabrics. The woven fabrics are tested forEMSE and UV resistance, examining the effect of the lamination-layer numbers and lamination-layer angles. Test resultsshow that the optimal EMSE and UV resistance occur when SS/PP woven fabrics are laminated with two layers at 90 °. Notonly focus on the mechanical performance, the proposed woven fabrics with good EMSE, UV resistance, and a light weight,and are good candidate for a variety of application as required. The proposed UV resistance and EMSE woven fabricssignificantly increase the additional values of traditional textiles.

Fabrication of a Biodegradable Multi-layered Polyvinyl Alcohol Stent

Mei-Chen Lin,Ching-Wen Lou,Jan-Yi Lin,Ting An Lin,Yueh-Sheng Chen,Jia-Horng Lin 한국섬유공학회 2018 Fibers and polymers Vol.19 No.8

This study aims to develop biodegradable vascular stents that prevent permanent damage caused by rejection of the immune system of the human body. Polyvinyl alcohol (PVA) yarns are twisted to enhance their strength. The twisted yarns are braided and then coated with chitosan (CS). The CS-coated PVA vascular stents are chemically crosslinked with genipin (GP) to improve their flexibility and biodegradability. Their morphological characteristics are also observed using a stereoscopic microscope, and their properties are evaluated through scanning electron microscopy, Fourier transform infrared, bending test, biodegradability test, drug release measurement, and MTT assay. Results reveal that wet PVA-CS-GP vascular stents coated with multiple CS layers can maintain a tubular structure when they are bent. After crosslinking is performed, the compressive strength of the PVA-CS-GP stents is 17.04 times higher than that of pure PVA. The weight loss rate of the PVA-CS-GP vascular stents as <3 % after 30 days. The PVA-CS-GP vascular stents composed of 0.10 % heparin sodium show a good drug release effect. Biological activity test indicates that these stents exhibit good proliferation, and our structural model verifies that they are good vascular stents.

Polyester/Polylactic Acid/Stainless Steel Composite Bone Scaffolds Made by Electrochemical Treatment: Process Design and Property Evaluations

Mei-Chen Lin,Ching-Wen Lou,Jan-Yi Lin,Ting An Lin,Shih-Peng Wen,Jia-Horng Lin 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

This study combines and twists 75D polyester (PET) multi-filaments and polylactic acid (PLA) multi-filaments with twist coefficients of 2, 3, 4, 5, and 6 to form 150D PET/PLA plied yarns. The 0.08-mm-diameter stainless steel (SS) fibers are made into SS braids with a 60-tooth braid gear and a take-up gear with 60, 70, 80, 90, or 100 teeth. PET/PLA plied yarn and SS braids are then combined and electrochemically treated with an electric current of 100, 200, 300, 400, or 500 mA at 60 ℃ for 24 hours, forming the PET/PLA/SS composite bone scaffolds. PET/PLA/SS composite bone scaffolds are observed by scanning electron microscope (SEM) and energy dispersive spectroscope (EDS), and tested for weight increase rate and biocompatibility. The experiment results show that the optimal twist coefficient for PET/PLA plied yarn is 4 and the optimal tooth number on the take-up gear for SS braids is 80. SEM observation result shows that hydroxyapatite (HA) deposits on the surface of PET/PLA/SS composite bone scaffolds and attaches to the PET/PLA plied yarns. Finally, regardless of electric currents, all PET/PLA/SS composite bone scaffolds possess good biocompatibility.

Taurine Attenuates Hepatic Inflammation in Chronic Alcohol-Fed Rats Through Inhibition of TLR4/MyD88 Signaling

Chao-Jen Lin,Chun-Ching Chiu,Yi-Chen Chen,Mu-Lin Chen,Tsai-Ching Hsu,Bor-Show Tzang 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.12

Accumulating evidence indicates that overconsumption of ethanol contributes in many ways to the pathogenesis of hepatic injury. Although studies indicate that taurine decreases lipogenesis, oxidative stress, and inflammatory cytokines, the protective effect of taurine against alcohol-induced liver injury is still unclear. To clarify the precise signaling involved in the beneficial effect of taurine on alcohol-induced liver injury, rats were randomly divided into four treatment groups: (1) control (Ctl), (2) alcohol (Alc), (3) Alc + taurine (Tau), and (4) Alc + silymarin (Sil). The Tau and Sil groups had lower lymphocyte infiltration and significantly lower TLR-4/MyD88 and IκB/NFκB compared to the Alc group. The inducible nitric oxide synthase (iNOS), C-reactive protein (CRP), tumor necrosis factors (TNF)-α, interleukin (IL)-6, and IL-1β were also significantly lower in the Tau and Sil groups than in the Alc group. The experimental results indicated that hepatoprotection against alcohol-induced inflammation may be mediated by decreased TLR-4/MyD88 signaling.