NEDD8-activating enzyme inhibitor, MLN4924 (Pevonedistat) induces NOXA-dependent apoptosis through up-regulation of ATF-4

Liu, Xiaojun,Jiang, Yanan,Wu, Jianfu,Zhang, Wenjuan,Liang, Yupei,Jia, Lijun,Yu, Jinha,Jeong, L.S.,Li, Lihui Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>It has been reported that MLN4924 can inhibit cell growth and metastasis in various kinds of cancer. We have reported that MLN4924 is able to inhibit angiogenesis through the induction of cell apoptosis both in vitro and in vivo models. Moreover, Neddylation inhibition using MLN4924 triggered the accumulation of pro-apoptotic protein NOXA in Human umbilical vein endothelial cells (HUVECs). However, the mechanism of MLN4924-induced NOXA up-regulation has not been addressed in HUVECs yet. In this study, we investigated how MLN4924 induced NOXA expression and cellular apoptosis in HUVECs treated with MLN4924 at indicated concentrations. MLN4924-induced apoptosis was evaluated by Annexin V-FITC/PI analysis and expression of genes associated with apoptosis was assessed by Quantitative RT-PCR and western blotting. As a result, MLN4924 triggered NOXA-dependent apoptosis in a dose-dependent manner in HUVECs. Mechanistically, inactivation of Neddylation pathway caused up-regulation of activating transcription factor 4 (ATF-4), a substrate of Cullin-Ring E3 ubiquitin ligases (CRL). NOXA was subsequently transactivated by ATF-4 and further induced apoptosis. More importantly, knockdown of ATF-4 by siRNA significantly decreased NOXA expression and apoptotic induction in HUVECs. In summary, our study reveals a new mechanism underlying MLN4924-induced NOXA accumulation in HUVECs, which may help extend further study of MLN4924 for angiogenesis inhibition treatment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MLN4924 triggered NOXA-dependent apoptosis in a dose-dependent manner in HUVECs. </LI> <LI> Inactivation of neddylation caused up-regulation of ATF4, a substrate of CRL. </LI> <LI> NOXA was transactivated by ATF4 and further induced apoptosis. </LI> <LI> Knockdown of ATF-4 significantly decreased NOXA expression and apoptotic induction. </LI> <LI> We revealed a new mechanism underlying MLN4924-induced NOXA accumulation in HUVECs. </LI> </UL> </P>

A reagent-assisted method in SERS detection of methyl salicylate

Li, Yali,Li, Qianwen,Wang, Yanan,Oh, Joohee,Jin, Sila,Park, Yeonju,Zhou, Tieli,Zhao, Bing,Ruan, Weidong,Jung, Young Mee Elsevier 2018 Spectrochimica acta. Part A, Molecular and biomole Vol.195 No.-

<P><B>Abstract</B></P> <P>With the explosive application of methyl salicylate (MS) molecules in food and cosmetics, the further detection of MS molecules becomes particularly important. Here we investigated the detection of MS molecules based on surface-enhanced Raman scattering (SERS) in a novel molecule/assistant/metal system constructed with MS, 4,4′-(hexafluoroisopropylidene) bis (benzoic acid) and Ag nanoparticles (AgNPs). The minimum detection concentration is 10<SUP>−4</SUP> M. To explore the function of assisted reagent, we also referred another system without assistant molecules. The result demonstrates that SERS signals were not acquired, which proves that the assistant molecules are critical for the capture of MS molecules. Two possible mechanisms of MS/assistant/AgNPs system were speculated through two patterns of hydrogen bonds. The linker molecules acted as the role of the bridge between metallic substrates and target molecules through the molecular recognition. This strategy is very beneficial to the expanding of MS detection techniques and other hydrogen bond based coupling detections with SERS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel molecule/assistant/metal system was constructed to achieve the SERS detection of methyl salicylate. </LI> <LI> The intermolecular hydrogen bond was employed as the driving force to accomplish the assembly. </LI> <LI> The obstacle of non-adsorption of target molecules on substrates was solved through the hydrogen-bond-based assembly method. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Identification of native charge-transfer status of p-aminothiolphenol adsorbed on noble metallic substrates by surface-enhanced infrared absorption (SEIRA) spectroscopy

Li, Qianwen,Wang, Yanan,Li, Yali,Park, Yeonju,Chen, Yujing,Wang, Xu,Zhao, Bing,Ruan, Weidong,Jung, Young Mee Elsevier 2018 Spectrochimica acta. Part A, Molecular and biomole Vol.204 No.-

<P><B>Abstract</B></P> <P>p-Aminothiophenol (PATP) is a preferred molecule in research on surface-enhanced Raman scattering (SERS) because of its unique characteristics of high spectral activity, easily induced charge-transfer (CT), and sensitivity to molecular structural changes. However, some aspects are still unclear, such as the initial steady state of PATP on noble metallic substrates without strong additional excitation with incident and/or induced electromagnetic radiation. Information about the initial steady state, especially the intrinsic CT state, is of great importance to elucidate the dynamic processes of CT and/or molecular structural changes under additional excitation. To investigate the native state of an adsorbed molecule, a suitable probe method that does not disrupt the native state of the whole system, including both molecules and substrates, is required. SERS is not applied in this context because of its use of high-energy visible and near-infrared light. Herein, a low-energy probe method, surface-enhanced infrared-absorption (SEIRA) spectroscopy, is employed as a suitable method for studying the native adsorption state of PATP on silver nanoisland films. The molecular structure and adsorption state were investigated. The intrinsic CT state received particular attention by analyzing the CT-related vibration of B<SUB>2</SUB> modes. Using Fourier transform infrared (FTIR), transmission SEIRA and reflection SEIRA spectroscopy, we explained why the relative intensities of some bands were different under different conditions. A quasi-standing orientation of PATP adsorbed on the substrates was also confirmed. More importantly, we demonstrated that there is no perceptible CT between PATP and silver nanoisland films; in contrast, CT generally occurs in a disruptive manner in SERS. Density functional theory (DFT) calculations and the selection rules for infrared (IR) transmission and reflection-absorption spectroscopy were used to analyze the spectra throughout the paper. SEIRA proved to be an effective technique to explore the native adsorption state of molecules without the excessive external disturbance induced by excitation. The results are very important in providing insight into molecules in surface-interface chemistry, enhanced spectroscopy and photoelectronics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The native state of PATP was investigated when adsorbed on silver substrates. </LI> <LI> FTIR and SEIRA allow a probe method with less interference on surfaces. </LI> <LI> The intrinsic charge-transfer state was analyzed through the vibration modes of B<SUB>2</SUB>. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Cholesteryl naphthalimide-based gelators: Their applications in the multiply visual sensing of CO₂ based on an anion-induced strategy

Zhang, Xin,Li, Haimiao,Mu, Huifang,Liu, Yifan,Guan, Yanan,Yoon, Juyoung,Yu, Haitao Elsevier 2017 Dyes and pigments Vol.147 No.-

<P><B>Abstract</B></P> <P>Several cholesteryl naphthalimide-derived compounds have been sythesized and developed as CO<SUB>2</SUB> sensors. The derivatives, incorporating ether chains, were found to be capable of gelating various organic solvents, due to the modulation of the hydrophilicity/hydrophobility properties of them. The resulting organogels were well characterized by field emission scanning electron microscopy (FE-SEM), FT-IR spectroscopy, concentration- and temperature-dependent <SUP>1</SUP>H NMR spectra, and powder X-ray diffraction (PXRD). These gelators can be applied as fluorescent sensors for CO<SUB>2</SUB> in DMSO solutions via the anion-triggered strategy. Furthermore, the resulting organogelation systems in DMSO could also provide visual sensing means for CO<SUB>2</SUB> via the reversible solution-to-gel transition. Particularly, the re-gelation of DMSO induced by CO<SUB>2</SUB> bubbling could be directly observed without the aid of the heating-and-cooling operation. And the reversible sensing process could be repeated several times with the excellent chemical stability of the gelation system after the repeated stimuli of fluoride anion, CO<SUB>2</SUB> and N<SUB>2</SUB> with heating. These investigations indicate the developed organogelators own the applicable potential in the CO<SUB>2</SUB> detection.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Two new cholesteryl naphthalimide-based organogelators are developed for CO<SUB>2</SUB> sensing based on an anion-induced strategy. </LI> <LI> Sensors <B>1b</B> and <B>1c</B> exhibited optically ratiometric detection effects for CO<SUB>2</SUB> in solution with relatively low detection limits. </LI> <LI> Gelator <B>1c</B> can provide a visual sensing means for CO<SUB>2</SUB> directly via solution-gel transition. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Design and Construction of Polyaniline/Reduced Graphene Oxide Three-Dimensional Dendritic Architecture on Interdigital Electrode for Sensitive Detection Nitrite

Li Li,Huan Liu,Boya Li,Yanan Guo,Liming Qing,Baohui Wang 한국고분자학회 2020 Macromolecular Research Vol.28 No.5

The polyaniline/reduced graphene oxide (PANI/RGO) modified interdigital electrode (IDE) has been successfully fabricated by in situ electrochemical reduction and electrochemical polymerization through cyclic voltammetry. The morphology and topography of PANI/RGO characterized by SEM and AFM display intercrosslinked dendritic structure in three dimensions, and it is favorable for the detection of nitrite due to its large surface area, which can provide the large electrocatalytic active surface and various diffusion paths for nitrite. Herein, the obtained PANI/ RGO/IDE was employed for the electrochemical monitoring platform of nitrite for the first time and the electrochemical performance of the as-developed sensor was investigated via cyclic voltammetry and chronoamperometry. At the optimum conditions, the PANI/RGO/IDE has a linear response in the range from 0.4 to 183.7 mM with a sensitivity of 457.4 μA mM-1 cm-2 and a detection limit of 0.1 μM. Moreover, the obtained PANI/RGO/IDE with excellent long-term stability and reproducibility also can be employed for practical application for the determination of nitrite in tap water, the results show that the recovery rate is desirable. It is expected that IDE can be employed as the substrate electrode decorated with various materials to construct highperformance electrochemical sensors.

Influence of water injection parameters on the performance of a water-lubricated single-screw air compressor

Yanan Li,Jingfu Wang,Yuting Wu,Biao Lei,Ruiping Zhi,Lili Shen 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1

A thermodynamic model of a water-lubricated single-screw air compressor was established to examine the impact of water injection parameters on performance. Heat transfer and leakage between humid air and water was considered by analyzing the impacts of rotation speed, discharge pressure, the rate of water injection, and diameter of droplets on the performance of a compressor. The discharge temperature could be reduced by increasing the rate of water injection, resulting in the compression procedure moving towards an isothermal state. The increase in the rate of water injection under rated conditions from 60 L/min to 80 L/min resulted in a reduction in the compressor discharge temperature, increased the volume efficiency, and increased adiabatic efficiency by 11.1 K, 1.5 %, and 2.8 %, respectively. Water injection atomization increased the area of transfer of heat between humid air and water and improved the performance of compressor.

One-Step Hydrothermal Synthesis of Bi2S3-TiO2-RGO Composites with Enhanced Visible Light Photocatalytic Activities

Yanan Li,Zhongmin Liu,Yaru Li,Yongchuan Wu,Jitao Chen,Yanjun Liu,Ping Na 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.5

The Bi2S3-TiO2-RGO composites were synthesized by a facile one-step hydrothermal method and applied for the photocatalytic degradation of Rhodamine B (Rh B) under the visible light. The Bi2S3-TiO2-RGO composites were characterized by transmission electron microscopy, X-ray diffraction, Raman and Fourier transform infrared spectrometer. The results indicated that the Bi2S3-TiO2-RGO composites were successfully prepared, and Ti-O-C and S-C bonds were existing among Bi2S3, TiO2 as well as RGO. Furthermore, the photocatalytic ability of Bi2S3-TiO2-RGO composites was excellent under visible light due to its responding to the whole visible light region, low recombination rate of photogenerated electron–hole pairs and relatively negative conduction band. Rh B photocatalytic degradation rate was 99.5% after 50 min and still could reach 98.4% after five cycles. Finally, a formation mechanism as well as a photocatalytic mechanism of Bi2S3-TiO2-RGO composites were proposed based on the experimental results.

Multilayer-Structured Poly-Vanadium Acid/Polyaniline Composite: Synthesis and Properties for Humidity Sensing

Li Li,Yanan Guo,Chao Zhao,Liyuan Song 한국고분자학회 2018 Macromolecular Research Vol.26 No.7

A high-performance humidity sensor based on multilayer-structured poly-vanadium acid/polyaniline (V/PANI) composite was reported in this paper. Two-layer-structured V/PANI composite was fabricated by dip-coating poly-vanadium acid and electrochemically polymerizing PANI onto the interdigitated gold electrode in sequence, and then the process could be repeated to prepare the second two-layer-structured V/PANI composite. The crystalline phase properties and structural characteristics of the poly-vanadium acid, PANI, and V/PANI composite were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, and UV-visible spectroscopy, respectively. The morphological characteristics of the first two-layer-structured V/PANI composite and multilayer-structured V/PANI composite were characterized by scanning electron microscopy. Additionally, sensors based on the multilayer-structured V/PANI composite showed good humidity sensing properties. For instance, its impedance changed linearly for approximately four orders of magnitude in a wide range of 11-97% RH. Furthermore, it displayed small hysteresis (∼5%RH), fast response (t90% of 8 s and 12 s for adsorption and desorption processes, respectively), good stability. The possible sensing mechanism was analyzed by considering the special multilayer structure and using complex impedance spectra and the corresponding equivalent circuit of the sensor. The measurement results highlight the multilayer-structured V/PANI composite film is a candidate material for constructing humidity sensors.

Enhancing the Thermo-Stability and Anti-Biofilm Activity of Alginate Lyase by Immobilization on Low Molecular Weight Chitosan Nanoparticles

Li, Shangyong,Wang, Yanan,Li, Xiao,Lee, Beom Suk,Jung, Samil,Lee, Myeong-Sok MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.18

<P>Bacterial biofilm causes severe antibiotic resistance. An extracellular polymeric substance (EPS) is the main component in the bacterial biofilm. Alginate is a key EPS component in the biofilm of Pseudomonas aeruginosa and responsible for surface adhesion and stabilization of biofilm. Alginate lyase has emerged as an efficient therapeutic strategy targeting to degrade the alginate in the biofilm of P. aeruginosa. However, the application of this enzyme is limited by its poor stability. In this study, chitosan nanoparticles (CS-NPs) were synthesized using low molecular weight chitosan and alginate lyase Aly08 was immobilized on low molecular weight chitosan nanoparticles (AL-LMW-CS-NPs). As a result, the immobilization significantly enhanced the thermal stability and reusability of Aly08. In addition, compared with free Aly08, the immobilized AL-LMW-CS-NPs exhibited higher efficiency in inhibiting biofilm formation and interrupting the established mature biofilm of P. aeruginosa, which could reduce its biomass and thickness confirmed by confocal microscopy. Moreover, the biofilm disruption greatly increased the antibiotic sensitivity of P. aeruginosa. This research will contribute to the further development of alginate lyase as an anti-biofilm agent.</P>

Effects of essential oil coated with glycerol monolaurate on growth performance, intestinal morphology, and serum profiles in weaned piglets

Wang Yanan,Li Juntao,Wang Hua Kai,Mi Yihai,Xue Yongqiang,Li Jiaan,Ma Yongxi 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.5

Objective: This study aimed to investigate the effects of essential oil coated with glycerol monolaurate (GML) on the growth performance, intestinal morphology, and serum profiles of weaned piglets. Methods: A total of 144 weaned piglets (Duroc×[Landrace×Yorkshire], average weight 8.07±3.33 kg) were randomly assigned to three groups with six replicate pens and eight piglets per pen: i) CON: a corn-soybean basal diet; ii) LEG: with 1,000 mg/kg essential oil coated with GML; and iii) HEG: with 2,000 mg/kg essential oil coated with GML. Results: Results showed that average daily gain was increased (p<0.05) linearly by essential oil coated with GML supplementation on day 14 to 28 and day 0 to 28 compared with the CON group. Dietary supplementation with HEG increased (p<0.05) total antioxidant capacity and catalase activity on day 14, and immunoglobulin A (IgA) and IgM concentration on day 28 and tended to increase IgG on day 28. In addition, the crypt depth in the jejunum was reduced (p<0.05), and villus height and villus height/crypt depth in the ileum were increased (p<0.05) in the HEG group compared with the CON group. Moreover, lower (p<0.05) concentrations of tumor necrosis factor-α, interferon-γ, interleukin-1β (IL-1β), IL-8, and IL-10 were observed in the jejunum of piglets supplemented with HEG compared with the CON group. In addition, dietary HEG tended to decrease IL-6 level in the jejunum of piglets compared with the CON group. Conclusion: Dietary essential oil coated with GML can improve growth performance of weaned piglets. Moreover, supplementing 2,000 mg/kg essential oil coated with GML was demonstrated to improve antioxidant ability, and intestinal morphology, and reduce jejunal inflammatory factor levels.