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- 저자 : Boya Li (검색결과 2 건)
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Li Li,Huan Liu,Boya Li,Yanan Guo,Liming Qing,Baohui Wang 한국고분자학회 2020 Macromolecular Research Vol.28 No.5
The polyaniline/reduced graphene oxide (PANI/RGO) modified interdigital electrode (IDE) has been successfully fabricated by in situ electrochemical reduction and electrochemical polymerization through cyclic voltammetry. The morphology and topography of PANI/RGO characterized by SEM and AFM display intercrosslinked dendritic structure in three dimensions, and it is favorable for the detection of nitrite due to its large surface area, which can provide the large electrocatalytic active surface and various diffusion paths for nitrite. Herein, the obtained PANI/ RGO/IDE was employed for the electrochemical monitoring platform of nitrite for the first time and the electrochemical performance of the as-developed sensor was investigated via cyclic voltammetry and chronoamperometry. At the optimum conditions, the PANI/RGO/IDE has a linear response in the range from 0.4 to 183.7 mM with a sensitivity of 457.4 μA mM-1 cm-2 and a detection limit of 0.1 μM. Moreover, the obtained PANI/RGO/IDE with excellent long-term stability and reproducibility also can be employed for practical application for the determination of nitrite in tap water, the results show that the recovery rate is desirable. It is expected that IDE can be employed as the substrate electrode decorated with various materials to construct highperformance electrochemical sensors.
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Li Shiyi,Sun Jiachen,Yang Jinxiu,Yang Yi,Ding Hongfan,Yu Boya,Ma Kui,Chen Minliang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Aging skin is characterized by a disturbed structure and lack of blood supply, which makes it difficult to heal once injured. ADSCs secrete large amounts of cytokines, which promote wound healing and vascular regeneration through paracrine secretion, and the number of cytokines can be elevated by hypoxic pretreating. However, the components of ADSCs are difficult to retain in wounds. Gelatin methacrylate (GelMA) is a photopolymerizable hydrogel synthesized from gelatin and has recently emerged as a potentially attractive material for tissue engineering applications. GelMA loaded with concentrated hypoxic pretreated ADSCs conditioned medium could provide a new method of treating wounds in aged skin.Primary ADSCs were isolated from human adipose tissue and characterized by flow cytometry and differentiation test. ADSCs in passages 4-6 were pretreated in the hypoxic and normoxic environments to collect conditioned medium, the conditioned medium was then concentrated to prepare concentrated ADSCs conditioned medium(cADSC-CM)(the one collected from ADSCs under hypoxia was called hypo-CM ,and the one from normoxia was called nor-CM). The concentration of cytokines was detected. After treated with cADSC-CM, the abilities of proliferation, migration, and tube formation of human umbilical vascular endothelial cells (HUVECs) were assayed, and Akt/mTOR and MAPK signal pathway was detected using western blotting. GelMA+hypo-CM hydrogel was prepared, and a comprehensive evaluation of morphology, protein release efficiency, degradation rate, mechanical properties, and rheology properties were performed. Full-thickness skin wounds were created on the backs of 20-month-old mice. After surgery, GelMA, GelMA+F12, GelMA+hypo-CM, and GelMA+nor-CM were applied to the wound surface respectively. H&E, Masson, and immunohistochemistry staining were performed, and a laser Doppler perfusion imager was used to evaluate the blood perfusion. The student’s t-test was used for analysis between two groups and a one-way analysis of variance (ANOVA) was used for analysis among multi groups.Our results revealed that 1) wounds in aged skin healed more slowly than that in young skin and exhibited poorer perfusion; 2) hypoxic pretreated ADSCs secreted more cytokines including VEGF by activating HIF1α; 3) hypo-CM promoted proliferation and migration of HUVECs through VEGF/Akt/mTOR and MAPK signal pathway; 4) GelMA-hypoCM accelerated wound healing and angiogenesis in aged skin in vivo.GelMA loaded with concentrated hypoxic pretreated adipose-derived mesenchymal stem cells conditioned medium could accelerate wound healing in aged skin by promoting angiogenesis.
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