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Down-regulation of SENP1 Expression Increases Apoptosis of Burkitt Lymphoma Cells
Huang, Bin-Bin,Gao, Qing-Mei,Liang, Wei,Xiu, Bing,Zhang, Wen-Jun,Liang, Ai-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Objective: To investigate the effect of down-regulation of Sentrin/SUMO-specific protease 1 (SENP1) expression on the apoptosis of human Burkitt lymphoma cells (Daudi cells) and potential mechanisms. Methods: Short hairpin RNA (shRNA) targeting SENP1 was designed and synthesized and then cloned into a lentiviral vector. A lentiviral packaging plasmid was used to transfect Daudi cells (sh-SENP1-Daudi group). Daudi cells without transfection (Daudi group) and Daudi cells transfected with blank plasmid (sh-NC-Daudi group) served as control groups. Flow cytometry was performed to screen GFP positive cells and semiquantitative PCR and Western blot assays were employed to detect the inference efficiency. The morphology of cells was observed under a microscope before and after transfection. Fluorescence quantitative PCR and Western blot assays were conducted to measure the mRNA and protein expression of apoptosis related molecules (caspase-3, 8 and 9). After treatment with $COCl_2$ for 24 h, the mRNA and protein expression of hypoxia inducible factor -$1{\alpha}$ (HIF-$1{\alpha}$) was determined. Results: Sequencing showed the expression vectors of shRNA targeting SENP1 to be successfully constructed. Following screening of GFP positive cells by FCM, semiqualitative PCR showed the interference efficiency was $79.2{\pm}0.026%$. At 48 h after transfection, the Daudi cells became shrunken, had irregular edges and presented apoptotic bodies. Western blot assay revealed increase in expression of caspase-3, 8 and 9 with prolongation of transfection (P<0.05). Following hypoxia treatment, mRNA expression of HIF-$1{\alpha}$ remained unchanged in three groups (P>0.05) but the protein expression of HIF-$1{\alpha}$ markedly increased (P<0.05). However, in the sh-SENP1-Daudi group, the protein expression of HIF-$1{\alpha}$ remained unchanged Conclusion: SENP1-shRNA can efficiently inhibit SENP1 expression in Daudi cells. SENP1 inhibition may promote cell apoptosis. These findings suggest that SENP1 may serve as an important target in the gene therapy of Burkitts lymphoma.
Simulated Annealing Utilization for Intelligent Reflecting Surfaces
Wen-Bin Li,Thu L. N. Nguyen,Yoan Shin 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.6
In this paper, we first formulate the path loss model of an intelligent reflecting surface (IRS) in terms of IRS reflection coefficients, then adopt a simulated annealing algorithm to determine a global maximum point of the path loss. Consequently, we can improve the performance of IRS-assisted communication system by avoiding such position deviations in practice. Simulation results show that an optimal solution can be achieved by implementing the proposed algorithm.
( Bin Yin ),( Chen Xi Hu ),( Zheng Chen ),( Wen Jun Zhao ),( Li Rong Wei ),( Yan Wen Zheng ),( Chao He ),( Yan Zeng ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.2
Vesicular Stomatitis Virus G Glycoprotein and ATRA EnhancedBystander Killing of Chemoresistant Leukemic Cells by HerpesSimplex Virus Thymidine Kinase/Ganciclovir
Study on Volume Stability of Chemical Foaming Cement Paste
Wen-bin Liu,Xiong Zhang 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.7
The paper is aimed at investigating the mechanism and cause of volume instability of chemical foaming cement paste. Volume stability were studied by introducing ultimate shear stress of the technical parameter. The research shows that the ultimate shear stress should be between 25 Pa and 40 Pa when the paste was mixed for 30 minutes, and the ultimate shear stress reached 75 Pa when the paste was mixed for 2 hours. The paste is not easy to collapse, sink. By force analyzing of the paste, mathematical model of the paste was established. The ultimate shear stress was controlled by water-binder ratio, quick setting agent and foam stabilizer. The result shows that the water-binder ratio is 0.54~0.58, the setting accelerator is 2% of cement content, and the foam stabilizer is 1.6% of cement content, which played an important role in the volume stability of the paste.
Wen Bin Hung,Juyn Ye Chen,Kien Wen Sung,Teng Ming Chen 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.3
The colloidal submicron needle-like Ba2SiO4 : Eu2+ phosphor coated on microtextured silicon surface has been used as a luminescent down-shifting (LDS) material for enhancing the power conversion efficiency of solar cells. Under a simulated onesun illumination, upon phosphor coating the solar cells showed an enhancement of the short-circuit current density (Jsc) increased from 34.00 to 34.48 mA/cm2 , meanwhile the power conversion efficiency (PCE) was enhanced from 15.13% to 15.36%. The related underlying mechanism for these enhancements can be attributed to photon down-shifting and light scattering effect through the investigation of PLE spectrum, reflectance, and external quantum efficiency. Our results indicate that the colloidal submicron needle-like Ba2SiO4 : Eu2+ phosphor not only act as LDS centers in the UV region but also serve as an antireflection coating for enhancing the light absorption in the visible regime.
Use Chains to Block DNS Attacks: A Trusty Blockchain-based Domain Name System
Wen-Bin Hsieh,Jenq-Shiou Leu,Jun-Ichi Takada 한국통신학회 2022 Journal of communications and networks Vol.24 No.3
The Internet has become one of the most importanttechnologies in the world, and hackers use various methods tolaunch cyber attacks to profit from it. Phishing is one of famoussocial engineering attacks, it is often used to steal user data,including login credentials and credit card numbers. Althoughthe Transport Layer Security certificate is used to verify the trustof websites, there are still a series of vulnerabilities. The demandfor trusted IP addresses has led a lot of research, including IPwhitelisting, DNS filtering and so on. However, these technologiesstill have many shortcomings. In view of this, we proposeda novel mechanism for verifying websites using blockchaintechnology. The URL and IP address of a permissioned websiteare recorded in blockchain through a specific smart contract. A DNS query is executed through a smart contract designedto avoid URL redirection attacks. With the help of immutablenature of blockchian, phishing websites can be detected. Themechanism will not add any load to users and provides tamperprooffunctions based on the characteristics of blockchain. Thecomparison of related works shows that the proposed mechanismis more secure. We also provided a reference implementationof the proposed mechanism on Ethereum Quorum simulationplatform, which proves the effectiveness and practicability of themechanism.
Research on the Correlation between Wavy Modeling of Garment and Fabric Properties
( Wen Bin Zhang ),( Wu Tuo ),( Jing Ru Liu ) 한국패션비즈니스학회 2004 패션 비즈니스 Vol.8 No.6
On the basis of garment spatial configuration technology, the correlation of fabric properties and garment modeling was studied in this paper. With abundant experimental data, practical modeling and the judgment of fabric properties and physical shape, we analyzed the change rules that restricting all kinds of parameters of fabric properties and garment modeling, and discussed the relation of wavy modeling and all correlated factors of fabric properties. The result showed the basis of choosing fabric for different style garment, which would help designers choosing fabric for modeling design or structure design.
지속가능 엔지니어링을 위한 PLM 시스템의 개념 설계에 관한 연구
Wen-Bin Zhao,노상도(Sang Do Noh) (사)한국CDE학회 2012 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2012 No.2
PLM, Product Lifecycle Management 는 초기 제품 기획 단계인 고객 요구사항 파악에서부터 유지관리, 복구의 마지막 단계까지 제품의 전 라이프 사이클에 걸쳐 관련된 정보를 통합적으로 관리하는 것으로, 신속한 제품 개발과 협업 달성 등 제조업이 당면한 다양한 요구를 만족시킬 수 있는 새로운 패러다임이다. 일반적으로 PLM 에서는 제조과정에서 발생 가능한 문제점을 사전에 파악하여 비용과 시간을 최소화하고, 기술과 서비스 지원을 통한 생산성 향상을 추구하고 있다. 그러나, 근래에 들어 산업화 이후 문명을 지탱하고 있는 자원의 일방적 채취와 소비가 더 이상 인간의 번영을 보장 할 수 없다는 인식이 확산되고 있으며, 화석 연료를 기반으로 하는 직선적인 생산 및 소비 형태의 한계와 문제점이 드러나고 있다. 그 결과 저탄소 녹색성장, 청정생산, 친환경 제품 등의 이슈화가 본격적으로 진행되고 있으며, 제조 기업에서도 지속가능한 발전은 향후 산업 발전의 주요한 추진 방향으로 잡게 되었다. 본 논문에서는 제조 기업에서 기존의 제품, 생산 엔지니어링과 함께 지속가능 엔지니어링 수행을 지원하는 새로운 PLM 의 정보모델을 연구하였으며, PLM 시스템의 개념 설계를 진행하였다.