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전재석(Jaeseok Jeon),박현배(Hyunbae Park),문희석(Heeseok Moon),곽수진(Sujin Kwag) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5
In this paper, we described about evaluation of human sensibility using park assist system of the commercial vehicle. The perpendicular parking and parallel parking have been carried out as a user test and subjects are classified as beginning drivers, drivers with less than 1 year experience and over 3 years experience. As a result, parking duration of beginning drivers and drivers with less than 1 year experience who use automatic parking assistance system is similar to drivers with over 3 years experience. However error occurred according to sensor position and distance between vehicle and parking space. And also it occurred that the vehicle leaves from parking line or does not recognize the parking space. Therefore, if we improve its completeness by compensating problems that came up from this evaluation and distribute the automatic parking assistance system, it is expected that the park assistance system is to help driver providing convenience of parking.
조예슬(Yeseul Jo),박수진(Sujin Park),노관평(Gwan Pyeong Roh) 한국환경보건학회 2018 한국환경보건학회지 Vol.44 No.4
Objectives: The occurrence characteristics of BTEXS and phytoncides were investigated by type of urban forest. Methods: Four types of urban green space (Hongneung Forest, Mt. Chunjang, residential park, and traffic island) and Gwangneung Forest were selected. Monitoring of phytoncides and BTEXS was conducted considering the activity times of urban residents (five times per day) using a Tenax TA tube and suction pump in June 2017 (one day). Results: Phytoncide concentrations were ranked as Gwangneung Forest>Hongneung Forest>Mt. Cheonjang> traffic island>residential park. Relatively high concentrations of phytoncides were also identified in the urban forest. There was no significant difference between Gwangneung Forest and the urban forest. BTEXS concentrations were ranked as traffic island>residential park>Hongneung Forest>Gwangneung Forest>Mt. Cheonjang. Traffic island and residential park showed high levels of BTEXS depending on the inflow of vehicles. The difference in concentration by time was significant for the traffic island in particular. Pollutant levels in Hongneung Forest were as low as in Gwangneung Forest. Conclusion: The concentrations of phytoncides and BTEXS were different by types of urban green space, and the potential for health and hygiene of urban forests were able to be investigated. This study is expected to provide as basic data for the creation of urban forest spaces in the future.
Park, Sujin,Yang, Kyung-Min,Park, Yuna,Hong, Eunji,Hong, Chang Pyo,Park, Jinah,Pang, Kyoungwha,Lee, Jihee,Park, Bora,Lee, Siyoung,An, Haein,Kwak, Mi-Kyung,Kim, Junil,Kang, Jin Muk,Kim, Pyunggang,Xiao, Korean Society of Cancer Prevention 2018 Journal of cancer prevention Vol.23 No.1
<P><B>Background</B></P><P>Smad3 linker phosphorylation plays essential roles in tumor progression and metastasis. We have previously reported that the mutation of Smad3 linker phosphorylation sites (Smad3-Erk/Pro-directed kinase site mutant constructs [EPSM]) markedly reduced the tumor progression while increasing the lung metastasis in breast cancer.</P><P><B>Methods</B></P><P>We performed high-throughput RNA-Sequencing of the human prostate cancer cell lines infected with adenoviral Smad3-EPSM to identify the genes regulated by Smad3-EPSM.</P><P><B>Results</B></P><P>In this study, we identified genes which are differentially regulated in the presence of Smad3-EPSM. We first confirmed that Smad3-EPSM strongly enhanced a capability of cell motility and invasiveness as well as the expression of epithelial-mesenchymal transition marker genes, <I>CDH2</I>, <I>SNAI1</I>, and <I>ZEB1</I> in response to TGF-β1 in human pancreatic and prostate cancer cell lines. We identified <I>GADD45B</I>, <I>CTGF</I>, and <I>JUNB</I> genes in the expression profiles associated with cell motility and invasiveness induced by the Smad3-EPSM.</P><P><B>Conclusions</B></P><P>These results suggested that inhibition of Smad3 linker phosphorylation may enhance cell motility and invasiveness by inducing expression of <I>GADD45B</I>, <I>CTGF</I>, and <I>JUNB</I> genes in various cancers.</P>
Arctic Primary Aerosol Production Strongly Influenced by Riverine Organic Matter
Park, Jiyeon,Dall’Osto, Manuel,Park, Kihong,Kim, Jung-Hyun,Park, Jongkwan,Park, Ki-Tae,Hwang, Chung Yeon,Jang, Gwang Il,Gim, Yeontae,Kang, Sujin,Park, Sanghun,Jin, Yong Keun,Yum, Seong Soo,Simó American Chemical Society 2019 Environmental science & technology Vol.53 No.15
<P>The sources of primary and secondary aerosols in the Arctic are still poorly known. A number of surface seawater samples-with varying degrees of Arctic riverine and sea ice influences-were used in a sea spray generation chamber to test them for their potential to produce sea spray aerosols (SSA) and cloud condensation nuclei (CCN). Our interdisciplinary data showed that both sea salt and organic matter (OM) significantly influenced the SSA production. The number concentration of SSA in the coastal samples was negatively correlated with salinity and positively correlated with a number of OM tracers, including dissolved and chromophoric organic carbon (DOC, CDOM), marine microgels and chlorophyll <I>a</I> (Chl-<I>a</I>) but not for viral and bacterial abundances; indicating that OM of riverine origin enhances primary aerosol production. When all samples were considered, transparent exopolymer particles (TEP) were found to be the best indicator correlating positively with the ratio number concentration of SSA/salinity. CCN efficiency was not observed to differ between the SSA from the various samples, despite differences in organic characteristics. It is suggested that the large amount of freshwater from river runoff have a substantial impact on primary aerosols production mechanisms, possibly affecting the cloud radiative forcing.</P> [FIG OMISSION]</BR>
Park, Sujin,Lim, Jung Mi,Park, Seon Hwa,Kim, Suree,Heo, Sukyeong,Balla, Tamas,Jeong, Woojin,Rhee, Sue Goo,Kang, Dongmin Elsevier 2019 FREE RADICAL BIOLOGY AND MEDICINE Vol.131 No.-
<P><B>Abstract</B></P> <P>Binding of epidermal growth factor (EGF) to its cell surface receptor induces production of H<SUB>2</SUB>O<SUB>2</SUB>, which serves as an intracellular messenger. We have shown that exogenous H<SUB>2</SUB>O<SUB>2</SUB> reversibly inactivates the phosphatidylinositol 4-phosphate [PtdIns(4)P] phosphatase Sac1 (suppressor of actin 1) at the Golgi complex of mammalian cells by oxidizing its catalytic cysteine residue and thereby increases both the amount of Golgi PtdIns(4)P and the rate of protein secretion. Here we investigated the effects of EGF on Sac1 oxidation and PtdIns(4)P abundance at the Golgi in A431 cells. EGF induced a transient increase in Golgi PtdIns(4)P as well as a transient oxidation of Sac1 in a manner dependent on elevation of the intracellular Ca<SUP>2+</SUP> concentration and on H<SUB>2</SUB>O<SUB>2</SUB>. Oxidation of Sac1 occurred at the Golgi, as revealed with the use of the Golgi-confined Sac1-K2A mutant. Knockdown of Duox enzymes implicated these Ca<SUP>2+</SUP>-dependent members of the NADPH oxidase family as the major source of H<SUB>2</SUB>O<SUB>2</SUB> for Sac1 oxidation. Expression of a Golgi-targeted H<SUB>2</SUB>O<SUB>2</SUB> probe revealed transient EGF-induced H<SUB>2</SUB>O<SUB>2</SUB> production at this organelle. Our findings have thus uncovered a previously unrecognized EGF signaling pathway that links intracellular Ca<SUP>2+</SUP> mobilization to events at the Golgi including Duox activation, H<SUB>2</SUB>O<SUB>2</SUB> production, Sac1 oxidation, and PtdIns(4)P accumulation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EGF elicits transient H<SUB>2</SUB>O<SUB>2</SUB> production at the Golgi by Ca<SUP>2+</SUP>-dependent Duox. </LI> <LI> This H<SUB>2</SUB>O<SUB>2</SUB> inactivates Sac1 at the Golgi by transiently oxidizing its catalytic Cys. </LI> <LI> Transient inactivation of Sac1 results in a transient increase in Golgi PtdIns(4)P. </LI> <LI> This new EGF signaling pathway links Ca<SUP>2+</SUP> to Golgi PtdIns(4)P via Duox-H<SUB>2</SUB>O<SUB>2</SUB>-Sac1. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
ERADication of EDEM1 occurs by selective autophagy
Sujin Park,Insook Jang,Jin Won Cho,Jürgen Roth 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Misfolded and misassembled glycoproteins are retained in the endoplasmic reticulum (ER) where they are exposed to the protein folding machinery and protein quality control. The Unfolded Protein Response is activated in response to an accumulation of unfolded or misfolded proteins in the lumen of the ER. Eventually, misfolded and mis-assembled glycoproteins are targeted for degradation by a process called ER-associated protein degradation (ERAD). EDEM1 is an ERAD component that interacts with misfolded luminal glycoproteins and routes them for dislocation. This is followed by their ubiquitination and proteasomal degradation. Although EDEM1 was proposed to be a lectin–like protein and to react with Man8GlcNAc2 oligosaccharides of glycoproteins, still very little is known about the turnover and degradation mechanism of EDEM1 and how this relates to the fate of its substrates. Previously, we reported that EDEM1 exits the ER by a vesicular non-COPII-mediated mechanism and becomes rapidly degraded by basal autophagy. Here, we provide detailed insight into the mechanism by which EDEM1 becomes degraded. After its dislocation to the cytosol, EDEM1 is apparently making complexes with the selective autophagy receptors p62, NBR1 and Alfy. We observed co-distribution of EDEM1 and selective autophagy receptors by double and triple confocal laser scanning immunofluorescence. By quantifying the relationship of EDEM1 and the selective autophagy receptors as visualized by confocal laser scanning immunofluorescence and double immunogold electron microscopy, dramatical changes were observed in HepG2 cells. Following inhibition of autophagy by wortmannin, the number and size of cytoplasmic clusters composed of EDEM1 and the selective autophagy cargo receptors dramatically increased and this aggregate formation was independent of the activity of HDAC6. We observed that deglycosylation of EDEM1 occurred by the action of the cytosolic peptide N-glycanase since treatment with inhibitors resulted in a strong increase in the amount of glycosylated EDEM1. Inhibition of cytosolic peptide N-glycanase also inhibited wortmannin-induced aggregation of EDEM1 and its complex formation with p62. This indicates that deglycosylation of EDEM1 is a prerequisite for subsequent ubiquitination and interaction with selective autophagy receptors. This demonstrates that the ERAD component EDEM1 itself undergoes ERAD involving selective autophagy