MLL4 Regulates the Progression of Non-Small-Cell Lung Cancer by Regulating the PI3K/AKT/SOX2 Axis

Yang Yang,Rongfang Qiu,Qiaoyou Weng,Ziwei Xu,Jingjing Song,Siyu Zhao,Miaomiao Meng,Dengke Zhang,Chunli Kong,Hailin Wang,Min Xu,Zhongwei Zhao,Jiansong Ji 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose Mixed-lineage leukemia protein 4 (MLL4/KMT2D) is a histone methyltransferase, and its mutation has been reported to be associated with a poor prognosis in many cancers, including lung cancer. We investigated the function of MLL4 in lung carcinogenesis. Materials and Methods RNA sequencing (RNA-seq) in A549 cells transfected with control siRNA or MLL4 siRNA was performed. Also, we used EdU incorporation assay, colony formation assays, growth curve analysis, transwell invasion assays, immunohistochemical staining, and in vivo bioluminescence assay to investigate the function of MLL4 in lung carcinogenesis. Results We found that MLL4 expression was downregulated in non–small cell lung cancer (NSCLC) tissues compared to adjacent normal tissues and tended to decrease with disease stage progression. We analyzed the transcriptomes in control and MLL4- deficient cells using high-throughput RNA deep sequencing (RNA-seq) and identified a cohort of target genes, such as SOX2, ATF1, FOXP4, PIK3IP1, SIRT4, TENT5B, and LFNG, some of which are related to proliferation and metastasis. Our results showed that low expression of MLL4 promotes NSCLC cell proliferation and metastasis and is required for the maintenance of NSCLC stem cell properties. Conclusion Our findings identify an important role of MLL4 in lung carcinogenesis through transcriptional regulation of PIK3IP1, affecting the PI3K/AKT/SOX2 axis, and suggest that MLL4 could be a potential prognostic indicator and target for NSCLC therapy.

Aerodynamic performance of high-lift blades in low-pressure turbines with periodic upstream wakes

Siyu Yang,Baopeng Xu,Fu Tian,Bin Wang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.5

The aerodynamic performance of high-lift blades was experimentally investigated at different Reynolds numbers ranging from 0.8×10 5 to 1.8×10 5 . Upstream wakes, inherent in real aero-engines, were generated by moving bars operating at reduced frequencies (Fr) of 0.3 and 0.6. Measurements were carried out by pneumatic probes and static pressure taps on the blade surfaces. The results show that high-lift blades experience a significant rise in profile loss under steady conditions, which is mitigated by upstream wakes due to the suppressed separation bubble. The loading distributions relate the non-dimensional flow deceleration rate (DR) to the profile loss. It is found that the variation pattern depends on the flow state, which is classified into parabolic increase, linear increase, and concave parabolic variation. A single hot-wire probe was employed to measure the boundary layer at the trailing edge. The results are used to examine the modified loss model based on Denton’s method.

The Development of Catering Giant Haidilao: The Leader in Quality Tracks

Li Xueyi,Jiang Xinmei,Zhang Siyu,Yang Xishe,Yang Yang Academy of Asian Business (AAB) 2022 Academy of Asian Business Review Vol.8 No.1

The purpose of this case study is to analyze the success story of Haidilao, a leading Chinese restaurant company known for its supreme service and innovative business model. The report is presented in three parts. The first part examines the history of Haidilao, discussing the background of its founding, its current position, and important turning points.The second section explores the factors behind Haidilao's success, categorized by internal management and external strategies. The unique corporate values of Asian companies that Haidilao possesses are highlighted. The third section discusses the internal and external challenges faced by Haidilao and the suggested solutions. Finally, this conclusion examines what we can learn from Haidilao's success. Against the backdrop of rapid changes in the world's restaurant industry, this case study hopes to provide insights for the emerging restaurant industry in Asia by analyzing the success story of a hot pot empire. Since Haidilao has a unique Asian-style business strategy in its development process, it is hoped that those Asian companies in the midst of globalization will take the best from the bad and learn from Haidilao's practices in industry chain layout, new technology application, and human resource management while learning valuable lessons from Haidilao's business process.

A Selective Colorimetric Sensor for Pb2+ Detection by Using Phenylboronic Acid Functionalized Polydiacetylene Liposomes

Siyu Zhang,Baoli Shi,Guang Yang 한국고분자학회 2020 Macromolecular Research Vol.28 No.1

Due to the high toxicity for brain and the nervous system of humans and other living organisms, the design and fabrication of a robust and sensitive method for the detection of Pb2+ in the aqueous solution has attracted the increasing attention among scientists. Herein, a colorimetric and fluorometric sensor for the detection of Pb2+ in the aqueous solution by incorporating a new phenylboronic acid functionalized polydiacetylene liposome was designed. The molar ratio of 4:1 between the monomers, 10,12-pentacosadiynoic acid (PCDA) and phenylboronic acid functionalized PCDA (PCDA-pBA) not only offered sufficient binding sites for Pb2+, but also interrupted the efficient π-conjugation backbone of polydiacetylene (PDA) chains associated with the color and fluorescence changes. Compared with other metal ions, only Pb2+ could cause the color change and the fluorescence enhancement. Moreover, the hybrid PDA liposomes exhibited high sensitivity, fast response time (10 s), and a reasonable detection limit for Pb2+ (0.1 μM). This work not only provides a versatile methodology for the fabrication of sensors for Pb2+ detection, but also is of great fundamental value for a deeper understanding of the complexion-induced color changes based on polydiacetylene materials, and may pave the way for fabrication a new method for recognizing other metal ions.

Lack of Association Between the Matrix Metalloproteinase-2 -1306C>T Polymorphism and Breast Cancer Susceptibility: a Meta-analysis

Yang, Lu,Li, Ning,Wang, Siyu,Kong, Yanan,Tang, Hailin,Xie, Xinhua,Xie, Xiaoming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: Since inconsistent results have been reported regarding the relation between the matrix metalloproteinase-2 (MMP-2) -1306C>T polymorphism and susceptibility for breast cancer, we performed a meta-analysis to investigate the issue. Materials and Methods: An internet search of PubMed and EMBASE was performed to identify eligible studies. Pooled odds ratios (ORs) with their corresponding confidence intervals (CIs) were calculated to evaluate any association between MMP-2 -1306C>T polymorphism and breast cancer susceptibility. Results: Nine case-control studies were included in the meta-analysis, involving 9,858 cases and 10,871 controls. Overall, there was no evidence of any association between the MMP-2 -1306C>T polymorphism and breast cancer susceptibility in different genetic models (T-allele vs C-allele: OR=0.95, 95%CI, 0.82-1.10, p=0.49; TT vs CC: OR=1.03, 95%CI, 0.90-1.19, p=0.66; TT+TC vs CC: OR=0.93, 95%CI, 0.78-1.10, p=0.38; TT vs TC+CC: OR=1.02, 95%CI, 0.89-1.17, p=0.77). In the subgroup analysis by ethnicity, CC was associated with a significant increase in breast susceptibility among Latin-Americans in the dominant model (OR=0.61, 95%CI, 0.40-0.93, p=0.02), but the association disappeared in other models. No significant association was observed among Europeans, East Asians and others in different genetic models. In the subgroup analysis by their source of controls, no significant association between MMP-2 -1306C>T polymorphism and breast cancer susceptibility was noted among population-based studies and hospital-based studies in different genetic models. Conclusions: The results of this meta-analysis suggest that MMP-2 -1306C>T polymorphism is not associated with breast cancer susceptibility, although the association among Latin-Americans in the dominant model was significant.

Sensitive Colorimetric Sensor for Lead Ions and VOCs Based on Histidine-Functionalized Polydiacetylene

Guang Yang,Ziwei Nie,Siyu Zhang,Zhaoliang Ge,Jiayin Zhao,Jiuru Zhang,Bin Li 한국고분자학회 2020 Macromolecular Research Vol.28 No.13

Due to the high toxicity of lead ions (Pb2+) and volatile organic compounds (VOCs) to human health and the environment, increasing attention has been paid by scientists to the development of simple, flexible and sensitive sensors or methods that are capable of tracing Pb2+ ions or VOCs with colorimetric visualization. In this study, the fabrication of colorimetric sensors based on histidine-functionalized diacetylene (His-DA), chromatic π-conjugated liposomes, for tracing Pb2+ ions was described. With a suitable molar ratio (4:1) between the10,12-pentacosadiynoic acid (PCDA) and His-DA monomers, the histidine units of the hybrid liposomes could rationally interrupt the efficient backbone length of polydiacetylene (PDA) chains, which causes the probe to be highly sensitive and selective for colorimetric visualization of tracing Pb2+ ions. Moreover, the His-PDA films also displayed sensitivity to volatile organic solvents or vapors, which could promote vivid color changes from blue to purple or pink. These interesting findings indicate that histidine-functionalized diacetylene may offer a promising way to design smart devices for real applications of sensing or tracing hazardous substances in the future.

The Preparation, Fluorescence Mechanism and Applications of High Efficiency Polymer Carbon Dots

Bai YANG,Shoujun ZHU,Siyu LU,Yubin SONG,Xiaohuan ZHAO 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Dynamic Mechanical Properties of Red Sandrock-Polypropylene Fiber Reinforced Concrete Composite under Impact Load

Liyun Yang,Fei Zhang,Huanzhen Xie,Siyu Chen,Changyu Lin 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.3

To investigate the dynamic mechanical properties and fracturing behavior of the red sandrock-polypropylene fiber reinforced concrete (R-PPFRC) composite, R-PPFRC composite specimens with different Polypropylene fiber (PPF) dosage were fabricated. Conducted quasi-static compression experiments and dynamic experiments by using Split Hopkinson pressure bar (SHPB) apparatus and testing machine. The fracture characteristics and process of composite specimens were analyzed with the assistance of ultra-high-velocity camera photography. It can be concluded that the dynamic increase factor and the dynamic compressive strength of composite specimens R-PPFRC increase with the increase of load level, indicating a remarkably strain rate strengthening effect. The optimal PPF content is determined finally. The dynamic impact toughness, ultimate toughness, and dissipation energy of R-PPFRC are significantly affected by the load level and the PPF content. The fracturing process of the composite specimen is divided into four phases, crack initiation, crack expansion, crack cluster and ultimate failure. After going through these phases, the ultimate failure pattern is mainly shear and split mixed failure.

Sedimentology, geological modeling and prediction of the remaining oil distribution in a complicated fault-block reservoir in the Weicheng Oilfield, Dongpu Depression, China

Jinkun Yang,Weilu Li,Siyu Gao,Jinliang Zhang,Yi Zhang,Jiawei Lu 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.5

Reservoirs in the Weicheng Oilfield, which occur in Paleogene Shahejie Formation Member 4 (Es4), were formed during the initial and strongly rifting stage of the Dongpu Depression. The geometry and distribution of sands of different types have controlled the heterogeneity of the reservoirs and have resulted in exploration challenges and relatively low recoveries. Based on various static and dynamic geological data including drilling, logging, seismic and production data, the microfacies features and distribution of Es4 were analyzed. Geostatistical three-dimensional (3-D) models and an integrated numerical simulation were constructed to quantify the geometry, spatial distribution and continuity of the reservoir sands and to predict the remaining oil distribution rules. Two depositional systems were recognized in Es4: terminal fans deposits in the lower part and partial shallow-lake deposits in the upper part. The three-dimensional geological model provided insights into the external and internal geometries, distributions and physical properties of the reservoir units. A sedimentary and connectivity feature analysis suggested four types of flow units: Type I units, which are primary sands of distributary channels and bars; Type II units, which are primary sands of proximal overbank and beaches; Type III units, which are sands of distal overbank; and Type IV units, which are mud flats and inter-beach-bar. The numerical simulation reproduced the changing process of the remaining oil, and the areas that have both high remaining oil saturations and reserve abundance, which are controlled by micro-facies and influenced by the structural trend and injection-production relations, comprise the key potential reservoirs: the sand pinch-out area, the imperfect pattern area and structural highs in Type I units and most of the areas in Type II units.

IL-17A Secreted by Th17 Cells Is Essential for the Host against Streptococcus agalactiae Infections

( Jing Chen ),( Siyu Yang ),( Wanyu Li ),( Wei Yu ),( Zhaowei Fan ),( Mengyao Wang ),( Zhenyue Feng ),( Chunyu Tong ),( Baifen Song ),( Jinzhu Ma ),( Yudong Cui ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.5

Streptococcus agalactiae is an important bacterial pathogen and causative agent of diseases including neonatal sepsis and meningitis, as well as infections in healthy adults and pregnant women. Although antibiotic treatments effectively relieve symptoms, the emergence and transmission of multidrug-resistant strains indicate the need for an effective immunotherapy. Effector T helper (Th) 17 cells are a relatively newly discovered subpopulation of helper CD4⁺ T lymphocytes, and which, by expressing interleukin (IL)-17A, play crucial roles in host defenses against a variety of pathogens, including bacteria and viruses. However, whether S. agalactiae infection can induce the differentiation of CD4⁺ T cells into Th17 cells, and whether IL-17A can play an effective role against S. agalactiae infections, are still unclear. In this study, we analyzed the responses of CD4⁺ T cells and their defensive effects after S. agalactiae infection. The results showed that S. agalactiae infection induces not only the formation of Th1 cells expressing interferon (IFN)-γ, but also the differentiation of mouse splenic CD4⁺ T cells into Th17 cells, which highly express IL-17A. In addition, the bacterial load of S. agalactiae was significantly increased and decreased in organs as determined by antibody neutralization and IL-17A addition experiments, respectively. The results confirmed that IL-17A is required by the host to defend against S. agalactiae and that it plays an important role in effectively eliminating S. agalactiae. Our findings therefore prompt us to adopt effective methods to regulate the expression of IL-17A as a potent strategy for the prevention and treatment of S. agalactiae infection.