Glucocorticosteroid가 흉선 세포의 아형 및 핵산에 미치는 영향

문성록,이시은,장선일,조성경,정헌택 최신의학사 1991 最新醫學 Vol.34 No.1

Shade guide의 형태가 색상 결정에 미치는 영향

박걸,김동준,이시은,황윤찬,오원만,황인남 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.3

This study was conducted in order to assess whether the form of the shade guide affects in deciding the color of the teeth using the shade guide. Eight shade light cured composite resins (Esthet-X, Dentsply, Milford, USA) were used in this study. Shade guides including the model of maxillary central incisors, teeth-form shade guide, doughnut form shade guide, and shade guide with perforated gray shield were prepared with eight shade composite resins and provided the codes randomly. After arranging the models of teeth, 19 dentists working at the clinic of the Dentistry of Chonnam University Hospital and 65 students of college of dentistry, Chonnnam University selected the shade guides corresponding to the color of each tooth on the gray board under the D_(65) standard illuminant. Bl shade showed highest accuracy of about 95% among all shade guides of 3 forms applied to the test and regardless of observer, tooth form shade guide showed the highest accuracy (p < 0.05) , and the doughnut form showed the lowest accuracy (p < 0.05). At the time of deciding on the color of the teeth using the shade guides as a result of above, the forms of the shade guides can affect the accuracy, and it suggests that the development of the diversified forms of shade guides, which may obtain more accurate results, is required. 본 연구에서는 shade guide를 이용하여 치아의 색상을 결정하는데 있어 shade guide의 형태가 미치는 영향을 평가 하기 위해 시행하였다 8가지 서로 다른 색상 code (Al, A2, Bl, B2, B3, C2, C3, D3)를 사용하는 광중합 복합레진 (Esthet-X, Dentsply, USA)을 이용하여 각 색상마다 상악 중절치 모형, 치아형 shade guide, 도넛형 shade guide, 및 회색 shield를 포함하는 shade guide를 제작하고 무작위로 기호를 부여하였다. 제작된 치아 모형을 배열하고 전남 대학교병원 치과 진료처에 근무하는 19명의 수련의와 전남대학교 치과대학 2, 3학년 학생 65명을 대상으로 회색 배경 판과 D_(65) 표준광 하에서 각 치아의 색상과 일치하는 shade guide를 선택하게 하였다. 연구 결과 Bl 색상은 실험에 적용한 3가지 형태의 모든 shade guide에서 약 95%의 가장 높은 정확도를 보였으며 , 색상간 색차가 가장 적은 B2와 C2는 3가지 형태의 모든 shade guide에서 서로 비슷한 정도의 교차 선택율을 보였다. 또한 관찰자에 상관없이 치아 형태의 shade guide는 가장 높은 정확도를 보인 반면 (p < 0.05), 도넛 형태의 shade guide는 가장 낮은 정확도를 보였다 (p <0.05)

Flagellin-adjuvanted cancer vaccine induces an excellent therapeutic efficacy in an orthotopic genital cancer model

Shee Eun LEE 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.10

Tetanus toxin fragment C fused to flagellin makes a potent mucosal vaccine

Lee, Shee Eun,Nguyen, Chung Truong,Kim, Soo Young,Thi, Thinh Nguyen,Rhee, Joon Haeng 대한백신학회 2015 Clinical and Experimental Vaccine Research Vol.4 No.1

<P><B>Purpose</B></P><P>Recombinant subunit vaccines provide safe and targeted protection against microbial infections. However, the protective efficacy of recombinant subunit vaccines tends to be less potent than the whole cell vaccines, especially when they are administered through mucosal routes. We have reported that a bacterial flagellin has strong mucosal adjuvant activity to induce protective immune responses. In this study, we tested whether FlaB could be used as a fusion partner of subunit vaccine for tetanus.</P><P><B>Materials and Methods</B></P><P>We constructed fusion proteins consisted with tetanus toxin fragment C (TTFC), the nontoxic C-terminal portion of tetanus toxin, and a Toll-like receptor 5 agonist from <I>Vibrio vulnificus</I> (FlaB). Mice were intranasally administered with fusion protein and protective immune responses of the vaccinated mice were analyzed.</P><P><B>Results</B></P><P>FlaB-TTFC recombinant protein induced strong tetanus-specific antibody responses in both systemic and mucosal compartments and prolonged the survival of mice after challenge with a supra-lethal dose of tetanus toxin.</P><P><B>Conclusion</B></P><P>This study establishes FlaB as a successful fusion partner for recombinant subunit tetanus vaccine applicable through mucosal route, and it further endorses our previous observations that FlaB could be a stable adjuvant partner for mucosal vaccines.</P>

A Bacterial Flagellin, Vibrio vulnificus FlaB, Has a Strong Mucosal Adjuvant Activity To Induce Protective Immunity

Lee, Shee Eun,Kim, Soo Young,Jeong, Byung Chul,Kim, Young Ran,Bae, Soo Jang,Ahn, Ouk Seon,Lee, Je-Jung,Song, Ho-Chun,Kim, Jung Mogg,Choy, Hyon E.,Chung, Sun Sik,Kweon, Mi-Na,Rhee, Joon Haeng American Society for Microbiology 2006 Infection and immunity Vol.74 No.1

<B>ABSTRACT</B><P>Flagellin, the structural component of flagellar filament in various locomotive bacteria, is the ligand for Toll-like receptor 5 (TLR5) of host cells. TLR stimulation by various pathogen-associated molecular patterns leads to activation of innate and subsequent adaptive immune responses. Therefore, TLR ligands are considered attractive adjuvant candidates in vaccine development. In this study, we show the highly potent mucosal adjuvant activity of a <I>Vibrio vulnificus</I> major flagellin (FlaB). Using an intranasal immunization mouse model, we observed that coadministration of the flagellin with tetanus toxoid (TT) induced significantly enhanced TT-specific immunoglobulin A (IgA) responses in both mucosal and systemic compartments and IgG responses in the systemic compartment. The mice immunized with TT plus FlaB were completely protected from systemic challenge with a 200× minimum lethal dose of tetanus toxin. Radiolabeled FlaB administered into the nasal cavity readily reached the cervical lymph nodes and systemic circulation. FlaB bound directly to human TLR5 expressed on cultured epithelial cells and consequently induced NF-κB and interleukin-8 activation. Intranasally administered FlaB colocalized with CD11c as patches in putative dendritic cells and caused an increase in the number of TLR5-expressing cells in cervical lymph nodes. These results indicate that flagellin would serve as an efficacious mucosal adjuvant inducing protective immune responses through TLR5 activation.</P>

Toll-Like Receptor Ligands as Cancer Immunotherapeutics

Shee Eun Lee,Joon Haeng Rhee 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.3

The pyrH Gene of Vibrio vulnificus Is an Essential In Vivo Survival Factor

Lee, Shee Eun,Kim, Soo Young,Kim, Choon Mee,Kim, Mi-Kwang,Kim, Young Ran,Jeong, Kwangjoon,Ryu, Hwa-Ja,Lee, Youn Suhk,Chung, Sun Sik,Choy, Hyon E.,Rhee, Joon Haeng American Society for Microbiology 2007 Infection and immunity Vol.75 No.6

<B>ABSTRACT</B><P>We have suggested an important role of the <I>pyrH</I> gene during the infectious process of <I>Vibrio vulnificus.</I> Previously, we have identified 12 genes expressed preferentially during human infections by using in vivo-induced antigen technology. Among the in vivo-expressed genes, <I>pyrH</I> encodes UMP kinase catalyzing UMP phosphorylation. Introduction of a deletion mutation to the <I>pyrH</I> gene was lethal to <I>V. vulnificus</I>, and an insertional mutant showed a high frequency of curing. We constructed a site-directed mutant strain (R62H/D77N) on Arg-62 and Asp-77, both predicted to be involved in UMP binding, and characterized the R62H/D77N strain compared with the previously reported insertional mutant. We further investigated the essential role of the <I>pyrH</I> gene in the establishment of infection using the R62H/D77N strain. Cytotoxicity was decreased in the R62H/D77N strain, and the defect was restored by an in <I>trans</I> complementation. The intraperitoneal 50% lethal dose of the R62H/D77N strain increased by 26- and 238,000-fold in normal and iron-overloaded mice, respectively. The growth of the R62H/D77N strain in 50% HeLa cell lysate, 100% human ascitic fluid, and 50% human serum was significantly retarded compared to that of the isogenic wild-type strain. The R62H/D77N mutant also had a critical defect in the ability to survive and replicate even in iron-overloaded mice. These results demonstrate that <I>pyrH</I> is essential for the in vivo survival and growth of <I>V. vulnificus</I> and should be an attractive new target for the development of antibacterial drugs and replication-controllable live attenuated vaccines.</P>

Flagellin is a strong vaginal adjuvant of a therapeutic vaccine for genital cancer

Lee, Shee Eun,Hong, Seol Hee,Verma, Vivek,Lee, Youn Suhk,Duong, Tra-My Nu,Jeong, Kwangjoon,Uthaman, Saji,Sung, Young Chul,Lee, Jae-Tae,Park, In-Kyu,Min, Jung-Joon,Rhee, Joon Haeng TaylorFrancis 2016 Oncoimmunology Vol.5 No.2

<P><B>ABSTRACT</B></P><P>Cervical cancer is a high-incidence female cancer most commonly caused by human papilloma virus (HPV) infection of the genital mucosa. Immunotherapy targeting HPV-derived tumor antigens (TAs) has been widely studied in animal models and in patients. Because the female genital tract is a portal for the entry of HPV and a highly compartmentalized system, the development of topical vaginal immunotherapy in an orthotopic cancer model would provide an ideal therapeutic. Thus, we examined whether flagellin, a potent mucosal immunomodulator, could be used as an adjuvant for a topical therapeutic vaccine for female genital cancer. Intravaginal (IVAG) co-administration of the E6/E7 peptides with flagellin resulted in tumor suppression and long-term survival of tumor-bearing mice. In contrast to IVAG vaccination, intranasal (IN) or subcutaneous (SC) immunization did not induce significant tumor suppression in the same model. The vaginal adjuvant effect of the flagellin was completely abolished in Toll-like receptor-5 (TLR5) knock-out mice. IVAG immunization with the E6/E7 peptides plus flagellin induced the accumulation of CD4<SUP>+</SUP> and CD8<SUP>+</SUP> cells and the expression of T cell activation-related genes in the draining genital lymph nodes (gLNs). The co-administered flagellin elicited antigen-specific IFNγ production in the gLNs and spleen. The intravaginally administered flagellin was found in association with CD11c<SUP>+</SUP> cells in the gLNs. Moreover, after immunization with a flagellin and the E6/E7 peptides, the TLR5 expression in gLN cells was significantly upregulated. These results suggest that flagellin serves as a potent vaginal adjuvant for a therapeutic peptide cancer vaccine through the activation of TLR5 signaling.</P>

Toll-Like Receptor Ligands as Cancer Immunotherapeutics

Lee, Shee Eun,Rhee, Joon Haeng 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.3

Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) expressed in a wide spectrum of cell types that recognize distinctive ligands and subsequently activate adaptive immune responses. TLR ligands are considered a promising target for development of immunomodulatory agents. Extensive clinical investigations are currently underway to develop TLR ligands-based non-specific immunostimulants and vaccine adjuvants. It has been well accepted that cancer cells develop a strategy to avoid host immune responses by producing inhibitory molecules. In addition, tumor-associated antigens are often not strong enough to induce effective anti-cancer immune responses. In this context, immunostimulants or adjuvants are critically required for more effective cancer immunotherapies. Here, we discuss recent progresses in the field of cancer immunotherapy under special emphasis on the TLR ligands as a component of immunostimulatory agents.

Poster Session 2 : Cellular signal transduction: hormones and mediators ; TNF-α is involved in osteoclast survival

( Shee Eun Lee ),( Han Bok Kwak ),( Hong Hee Kim ),( Zang Hee Lee ) 한국생화학분자생물학회 (구 한국생화학회) 2001 생화학분자생물학회 춘계학술발표논문집 Vol.2001 No.-