Inhibitory Effect of Cordycepin on Human Platelet Aggregation

박화진,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

Cordycepin separated from Cordyceps militaris is a major physiologic active component in Cordyceps militaris. The platelet aggregation is stimulated by Ca^(2+), which is either mobilized from intracellular endoplasmic reticulum or transported from extracellular space. cGMP antagonizes the actions of Ca^(2+). Based on these facts, we have investigated the effects of cordycepin on the mobilization of Ca^(2+) and the production of cGMP on collagen (10 ㎍/ml)-induced human platelet aggregation. Cordycepin potently stimulated the human platelet aggregation induced by collagen (10 ㎍/ml) in a dose-dependent manner. Cordycepin (500 μM) inhibited also the collagen-induced human platelet aggregation in the presence both 1 mM and 2 mM of CaCl_(2). These are in accord with the results that cordycepin inhibited the Ca^(2+)-influx on collagen-induced human platelet aggregation. These results suggest that cordycepin decrease the intracellular Ca^(2+) concentration to inhibit collagen-induced human platelet aggregation. Besides, cordycepin increased the level of cGMP on collagen-induced human platelet aggregation. This result is related with the decrease of intracellular Ca^(2+) concentration, because cGMP inhibits the mobilization of Ca^(2+). In addition, cordycepin inhibited the human platelet aggregation induced by LY-83583, inhibitor of guanylate cyclase. This result suggested that cordycepin inhibit the platelet aggregation by stimulating the activity of guanylate cyclase. In conclusion, we demonstrated that cordycepin might have the antiplatelet function by inhibiting Ca^(2+)-mobilization via the stimulation of the production of cGMP.

홍삼류의 섭취가 비만과 혈중 지질의 상호관계에 미치는 영향

박화진,이정희,이소진,함혜선,조현정,임창률,유영빈,박기현 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.4

비만은 동맥경화의 위험인자로서 혈중에 triglyceride의 농도를 종가시키고, 상대적으로 HDL-Cholesterol의 농도를 감소시킴과 동시에 수축기 혈압을 상승시킨다. 본 연구에서 홍삼제품류를 4년 또는 5년 동안 복용해 온 건강한 사람 (ginseng군)과 홍삼제품을 복용하지 않은 건강한 사람 (control군)을 대상으로 하여 신체 계측치로부터 비만지수를 구하고 이것과 혈중의 triglyceride (TG)농도, TG/HDL-Cholesterol ratio 및 수축기 혈압과의 상호관계를 연구한 바, ginseng군에서는 대조군에 비해 혈중의 TG 농도가 일정하게 유지되었고, TG/HDL-Cholesterol ratio 및 수축기 혈압도 일정하게 유지되었다. 이러한 현상은 ginseng군에서 기호품으로 alcohol을 섭취하거나, 흡연을 한 경우에도 상관없이 일정하게 유지 되었다. 이 결과는 홍삼제품류를 장기 복용하면 비만의 저하 및 고혈압 또는 동맥경화의 위험인자가 억제될 수 있고, 결론적으로 비만, 고혈압 및 동맥경화를 예방할 수 있을 것으로 추정한다. Obesity is the risk fcactor of atherosclerosis and not only increases triglyceride concentration in blood but also decreases relatively the ratio of TG to HDL-Cholesterol in blood. In case of obesity, systolic blood pressure is also increased in responding the increase of TG in blood. Index of obesity in red ginseng-taking group (ginseng group) was lower as compared with non-red ginseng-taking group (control group). The TG concentration, the ratio of triglyceride to HDL-cholesterol in blood and systolic blood pressure were decreased in the subjects of ginseng group compared with that in control group. It is inferred that long-term intake of ginseng products may help to prevent the risk of atherosclerosis and obesity.

Inhibitory Effect of Clavicepitaceae on Serotonin Release out of Human Platelets and Human Platelet Aggregation

박화진,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

We have investigated the effects of hypha-water extracts (HWE), fruit body-water extracts (FWE) and cordycepin from Cordyceps militaris on serotonin release out of human platelets and human platelet aggregation. HWE and FWE inhibited the release of [^(3)H]-serotonin from human platelet stimulated by thrombin (2 U/ml) or collagen (20 ㎍/ml) in a dose-dependent manner. Furthermore, cordycepin, a major component of Cordyceps militaris, inhibited the human platelet aggregation induced by collagen (10 ㎍/ml) in a dose-dependent manner. These results suggest that cordycepin containing in HWE and FWE may inhibit the serotonin release by suppressing the collagen-induced human platelet aggregation. Accordingly, our data demonstrate that HWE and FWE containing much cordycepin might have antithrombotic and antimigrainous functions.

교통사고에 의해 발생한 뇌내 좌상 1례

박혜연,전소분,김선아,정금선,조종기,송근호,이영원 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A two years old intact female Minature Schnauzer was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with an intermittent seizure by traffic accident. No specific finding was found by radiography and computer tomography (CT). Hematologic and blood chemical examination revealed that PCV (62.5%), BUN (72.6 mg/dl), CK (1769 U/l), ALT (169 U/l) and ALP (353 U/l) were increased. This dog was recovered by medical treatment.

코로나19 대응을 위해 한시적으로 허용된 전화상담·처방에 대한 병원급 의료공급자 인식과 만족도에 관한 연구

유혜림,김수민,박춘선,김지애 건강보험심사평가원 2021 HIRA RESEARCH Vol.1 No.1

.

유산소 리듬 운동프로그램이 노인의 혈압, 심박수 및 생활만족도에 미치는 효과

우선혜,김효정,박영숙 대한보건협회 2001 대한보건연구 Vol.27 No.3

The purpose of this study was to test the effects of aerobic rhythmical exercise program on blood pressure, pulse and the life satisfaction of the elderly. This quai-experimental study was designed as a non-equivalent control group pretest-posttest study. The subjects were 36 subjects(experimental group: 19 subjects, control group: 17 subjects), aged between 65 and 73 years who have normal cognition, sensory function and cerebellum function. Aerobic rhythmical exercise program was developed on the basis of aerobic exercise, Korean tradititional dance and music by the author. The blood pressure, pulse rate and life satisfaction were measured prior to and following the experimental treatment. Data were analyzed with x^2-test, t-test, mean, standard deviation, percentage of change and ANCOVA test using SAS program. The results were as follows. 1. There was no significant difference of systolic blood pressure between experimental and control group following the aerobic rhythmical exercise program(F=1.39, P=0.247). 2. The diastolic blood pressure of the experimental group was significantly lower than that of control group following the aerobic rhythmical exercise program(F=2.96, p<0.1). 3. There was no significant difference of resting heart rate between experimental and control group following the aerobic rhythmical exercise program(F=0.07, P=0.793). 4. The life satisfaction of the experimental group was significantly higher than that of control group following the aerobic rhythmical exercise program(F=9.94, p<0.01). On the basis of the above findings, aerobic rhythmical exercise program is effective in reducing diastolic blood pressure and promoting life satisfaction of the elderly. Thus, this program can be recommended as an effective nursing intervention for the elderly.

견비통의 한·양방 진료 및 협진의 경제성 평가

장혜정,홍상민,박유선,남동우,이두익,이재동,이윤호,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : The purpose of this study was to evaluate and compare the cost-effectiveness of Eastern, Western and collaborative treatments and suggest a cost-effective approach for patients with frozen shoulder pain. Methods : Using the data of fifty-two patients, treatment effectiveness was measured by CSA, SPADI, and ROM scales and changes from the baseline score were evaluated. Data source for cost estimation was based on the national health insurance (NHI) payment system. Because the price in NHI was differentiated by health care institutions, five collaborative types were considered in assessing costs. Cost-effective ratios were computed tor economic evaluation. Results : Compared with Eastem treatinent, collaborative and Westerm treatments showed better effects on CSA scale after 4 weeks' treatment. The collaborative approach was also the most effective treatment on SPADI and ROM scales The direct cost per patient receiving Eastern treatment was less than other treatments. In general, collaborative treatment dominated Eastern and Western treatments in cost-effectiveness analysis However, the cost-effectiveness ratio of Eastern treatment resulted in \9,0OO compared to W29,OOO of collaborative treatment on SPADI Four different indicators of ROM scales resulted in different approaches as the cost-effective treatment. Conclusiuns : Considering cost-effectiveness ratios, collaborative treatment was the best treatment on CSA and SPADl scales after 4 weeks' treatment As for ROM scales, the recommended altematives were Eastern treatment for patients with abduction and adduction disabilities, Western treatment for those with flexion disability, and collab-orative approach for those with extension disability.

일부 운수사업장 근로자의 건강진단 유소견자의 사후관리 실행과 관련요인

윤혜은,박종,이영선,강성득,김철호 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Objective : The purpose of this study was to find the relationship between the follow-up management status for medical problem to be treated and various characteristics of some workers at transportation business. Methods : A questionnaire survey was made toward 183 workers with medical problem(C and D2) among 945 examine in 1997. Results: Sixty four point five percent of surveyee received the examination result report and 40.5% were explained about the result 80.3% of surveyee answered that they knew the result of health examination. Most workers felt the necessity of the health examination, but 54.1% were dissatisfied with present health examination system and 66.7% pointed it out as nominal The rate of follow-up management for the medical problem was 62.8% By the multiple logistic regression the follow-up management rate for those who knew the health examination result were 2.98 times higher than those who didn't know the result (OR=2.98, 95% CI 1.41~6.29) The rate for those who perceived the medical problem already were 2.64 times higher than those who perceived the problem for the first time (OR=2.64, 95% CI 1.13~6.15). The rate for those who had subjective symptoms were 2.83 times higher than those who didn't have subjective symptoms. Conclusion : sixty two point eight percent of workers with medical problem performed the follow-up management and perception of health examination result, perception of the problem in the past and experience of subjective symptoms were related with the follow-up management.

청소년이 지각한 가족기능과 희망과의 관계

우선혜,노지영,박영숙 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: This Study a descriptive correlation survey with the purpose of identifying the relationship between family function and hope in adolescents. It was done to provide basic data for developing family centered nursing strategies to promote adolescents' hope. Methods: The subjects of this study were 1030 high school students in J city. Data were analyzed with SPSS 10.0 program. Frequencies and percentages were used to compare the subjects' general characteristics. Minimum values, maximum values, mean scores and mean grades were used to identify the level of family function and hope. Pearson's correlation coefficient was used to analyze the relationship between family function and hope in adolescents, and t test, ANOVA, and Scheffe test were used to examine the level of family function and hope by general characteristics. Results: 1. The level of adolescents' family function ranged from 26 to 97, the mean score was 66.85±13.16, and the mean grade was 2.76±0.52. 2. The level of adolescents' hope ranged from 55 to 153, the mean score was 111.48±17.64 and the mean grade was 2.78±0.44. 3. It showed that there is a significantly positive correlation (r=0.546, p=.000) between adolescents' family function and hope. That is, the better family function adolescents have, the higher level of hope they have. 4. Adolescents' family function showed significant differences according to school record (F=6.363, p=.002), school life satisfaction (F=4.922, p=.007), father's education (F=2.640, p=.048), mother's education (F=3.586, p=.013) and domestic economy status(F=3.022, p=.049). 5. Adolescents' hope showed significant differences according to religion (t=2.018, p=.044), school type (t=2.567, p=.010), school record (F=9.712, p=.000), school life satisfaction (F=11.367,p=.000), father's education (F=4.632, p=.003), mother's education (F=4.709, p=.003), domestic economy status (F=4.235, p=.015), relationship with friends (F=8.187, p=.000), and subjective health condition (F=5.490, p=.001). Conclusion: First, in order to improve adolescents' family function, which encourages hope in adolescents, it is necessary to develop nursing interventions and to make further studies. Second, it is necessary to develop instruments for improving Korean adolescents' family function and hope. Third, further studies are required to improve adolescents' family function and hope in special situations such as disease, drug abuse and juvenile delinquent.

乾癬患者 臨床報告

吳恩英,朴惠宣,池鮮渶,具德謨 동의대학교 한의학연구소 2001 동의ㆍ경산 한의학 학술대회 Vol.5 No.-

A Reports were done on proriasis which were treated by medications of Sasang Constitution and Constitution-Acupunture in Dept. surgery, opthalmology &tolaryngology, college of Oriental Medicine, Kyungsan University, Pohang, Korea, from May 30. 2000 to JUN 30 2001. we gave score and checked involvement, plaque, erythematous popules, invasiveness according to psoriasis area and severity index. The results were as follows: 1. Sasang Constitution classification was Soyanggin 14(93.33%), Taeumgin 1(6.67%) in the 15 cases. Hyungbangsabaeksan is used in 9 cases(60%), Yanggeuksanhoatang is used in 3 cases(13.11%), Geopungchunggisan is used in 3 cases(20%), Hyungbangdojucksan is used in 1 case(6.57%). 2. In the 9 cases, we checked for over 4 weeks, clinical severity of involvement, plaque, total score(psoriasis area and severity index) was siginificantly decreased(p<05). clinical severity of erythematous popules, itching was decreased but lt is not siginificant(p>05). 3. The mean duration of treatmemt was 73.3 days, result of Excellent or Good improvement was showed on over 120 days. These resilts indicate that Sasang Constitution and Constitution-Acupuntuer treatment is effetive on psoriasis and the more study is needed.