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- 저자 : ( Qiang Huang ) (검색결과 137 건)
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Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets
Huang Yahong,Wanqi Liang,Aihu Pan,Zhiai Zhou,Qiang Wang,Cheng Huang,Jianxiu Chen,Dabing Zhang 한국미생물학회 2006 The journal of microbiology Vol.44 No.5
FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli(ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, C83907 (K88ad, CT+, ST+). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.
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Qiang Huang,Yongwang Kang,Jinxia Song,Shiyu Qu,Yafang Han,Xiping Guo 대한금속·재료학회 2014 METALS AND MATERIALS International Vol.20 No.3
Nb-Si in situ composites with a nominal composition of Nb-22Ti-12Si-X (at.%, X represents Ni, Co, B, or Ge)are prepared using non-consumable arc melting technology. The effects of the alloying elements on themicrostructures and mechanical properties are investigated. The Nb-22Ti-12Si ternary alloy consists of Nbss andNb3Si. A new phase of Ti2Ni or Ti2Co is introduced into the ternary system after adding Ni or Co. The addition of6% Ge promotes the formation of Nb5Si3 and creates significantly finer microstructures. The values of the hightemperature strength and room temperature fracture toughness of Nb-22Ti-12Si-6Ge are 566.33MPa and12.81MPa·m1/2, respectively, which are approximately 88.2% and 18.5% higher than those of Nb-22Ti-12Si(300.98 MPa and 10.81 MPa·m1/2). The addition of 3% B changed the morphological features and inducedthe appearance of large fine rosettes, which is favorable for restraining the crack propagation, and it also leadsto a 22.3% increase in room temperature fracture toughness compared with the Nb-22Ti-12Si ternaryalloy.
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Huang, Qiang,Liu, Lei,Liu, Chen-Hai,Shao, Feng,Xie, Fang,Zhang, Chuan-Hai,Hu, San-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10
Background: There are few molecular markers known to predict cholangiocarcinoma (CCA) prognosis. Smad7 has a certain relationship with epithelial-mesenchymal transition (EMT), but its relevance to CCA in unclear. Therefore expression and clinical significance of Smad7 in CCA was the focus of this study. Methods: Expression of Smad7, E-cadherin and vimentin was assessed in 41 patients with CCA by immunohistochemistry and analyzed for associations with clinical parameters. Results: Smad7 and vimentin expression in the CCA tissue was dramatically higher than that in adjacent tissues. In addition, Smad7, vimentin and E-cadherin expression was significantly associated with CCA lymph node metastasis and perineural invasion($P{\leq}0.05$), but not other factors, such as gender, age, tumor location, tumor type and tumor differentiation degree (P>0.05). The overall survival and relapse-free survival rate was significantly higher in patients with negative Smad7 expression than those with positive Smad7 expression. Conclusion: EMT phenomena may occur in the process of CCA invasion and metastasis. Smad7, which was highly expressed in CCA, may be considered to be one feedback regulator in late stages and could have potential as a prognostic indicator for clinical assessment.
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Qiang Huang 아시아테플 2022 The Journal of Asia TEFL Vol.19 No.2
Language learning in classrooms has been studied from different perspectives. Among numerous focuses, the present study aims to examine three dimensions—classroom activities, classroom anxiety and teacher roles as well as their interactions by investigating 49 students of an English class at university. Quantitative instruments of three 5-likert scales were used to measure the number and the frequency of classroom activities used in the English class, the level of classroom anxiety of students as well as their perceptions of teacher roles. Results showed that the English class was “often” dominated by interactive activities, coupled “sometimes” with language exercises and “occasionally” with real-life tasks. In this activity-dominated English class, learners revealed rather low level of classroom anxiety. In contrast, the overall higher mean of teacher role perceived indicated noticeably facilitating impact of the teacher in class. Correlational analyses disclosed that classroom anxiety was neither significantly correlated with teacher role nor with classroom activity. However, a significant correlation was found between classroom activities and teacher roles.
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Huang, Qiang,Liu, Lei,Liu, Chen-Hai,You, Hao,Shao, Feng,Xie, Fang,Lin, Xian-Sheng,Hu, San-Yuan,Zhang, Chuan-Hai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Background: MicroRNAs are noncoding RNA molecules that posttranscriptionally regulate gene expression. The aim of this study was to determine the role of microRNA-21 in cholangiocarcinomas and its relationship to cholangiocarcinoma RBE cell capacity for invasion and metastasis. Methods: MicroRNA-21 expression was investigated in 41 cases of cholangiocarcinoma samples by in situ hybridization and real-time PCR. Influence on cholangiocarcinoma cell line invasion and metastasis was analyzed with microRNA-21 transfected cells. In addition, regulation of reversion-inducing-cysteine-rich protein with kazal motifs (RECK) by microRNA-21 was elucidated to identify mechanisms. Results: In situ hybridization and real-time quantitative PCR results for patients with lymph node metastasis or perineural invasion showed significantly high expression of microRNA-21 (P<0.05). There was a dramatic decrease in cholangiocarcinoma cell line invasion and metastasis ability after microRNA-21 knockdown (P<0.05). However, overexpression significantly increased invasion and metastasis (P<0.05). Real-time PCR and Western-blot analysis showed that microRNA-21 could potentially inhibit RECK expression in RBE cells. Survival analysis showed that patients with higher expression levels of microRNA-21 more often had a poor prognosis (P<0.05). Conclusions: MicroRNA-21 may play an important role in cholangiocarcinoma invasion and metastasis, suggesting that MicroRNA-21 should be further evaluated as a biomarker for predicting cholangiocarcinoma prognosis.
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Shuai Huang,Xiumin Xie,Qiang Xu,Xinhua Zhao,Guangwei Deng,Qiang Zhou,You Wang,Hai-Zhi Song 한국광학회 2020 Current Optics and Photonics Vol.4 No.6
A practical single photon source for fiber-based quantum information processing is still lacking. As a possible 1.55-µm quantum-dot single photon source, an InGaAsP/InP-air-aperture micropillar cavity is investigated in terms of fabrication tolerance. By properly modeling the processing uncertainty in layer thickness, layer diameter, surface roughness and the cavity shape distortion, the fabrication imperfection effects on the cavity quality are simulated using a finite-difference time-domain method. It turns out that, the cavity quality is not significantly changing with the processing precision, indicating the robustness against the imperfection of the fabrication processing. Under thickness error of ±2 nm, diameter uncertainty of ±2%, surface roughness of ±2.5 nm, and sidewall inclination of 0.5°, which are all readily available in current material and device fabrication techniques, the cavity quality remains good enough to form highly efficient and coherent 1.55-µm single photon sources. It is thus implied that a quantum dot contained InGaAsP/InP-air-aperture micropillar cavity is prospectively a practical candidate for single photon sources applied in a fiber-based quantum information network.
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Gao Huang,Weimin Zhang,Fei Meng,Zhangguo Yu,Xuechao Chen,Marco Ceccarelli,Qiang Huang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.7
In this paper, a master-slave control system is proposed and applied in an intention-actuated exoskeletal robot to assist user locomotion and lower extremity rehabilitation simultaneously. In particular, to increase users’ sense of participation, the motion of the exoskeleton and the wheelchair, which is denoted as slave motion in this study, is actuated by the user’s intention, which is denoted as master motion and thus makes patients feel that they are moving the wheelchair. This master-slave motion control system can help to eliminate patients’ fear of medical apparatus and instruments. The bicycling motion actuated by one motor is implemented to realize the rehabilitation motion exercise. Experimental results validate a position-force control strategy for the exoskeleton motors, and show that the proposed method can help users to move around and to exercise their legs simultaneously and effectively.
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Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets
Yahong, Huang,Liang, Wanqi,Pan, Aihu,Zhou, Zhiai,Wang, Qiang,Huang, Cheng,Chen, Jianxiu,Zhang, Dabing The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.5
FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, $C_{83907}$ (K88ad, $CT^+,\;ST^+$). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.
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Experimental investigation of multi-layered laminated glass beams under in-plane bending
Qiang Liu,Xiaokun Huang,Gang Liu,Zhen Zhou,Gang Li 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.60 No.5
Due to its relatively good safety performance and aesthetic benefits, laminated glass (LG) is increasingly being used as load-carrying members in modern buildings. This paper presents an experimental study into one applicational scenario of structural LG subjected to in-plane bending. The aim of the study is to reveal the in-plane behaviors of the LG beams made up of multi-layered glass sheets. The LG specimens respectively consisted of two, three and four plies of glass, bonded together by two prominent adhesives. A total of 26 tests were carried out. From these tests, the structural behaviors in terms of flexural stiffness, load resistance and post-breakage strength were studied in detail, whilst considering the influence of interlayer type, cross-sectional interlayer percentage and presence of shear forces. Based on the test results, analytical suggestions were made, failure modes were identified, corresponding failure mechanisms were discussed, and a rational engineering model was proposed to predict the post-breakage strength of the LG beams. The results obtained are expected to provide useful information for academic and engineering professionals in the analysis and design of LG beams bending in-plane.
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