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Peng Wu,Qian-qian Guo,Zhi-wei Qin 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.5
Pesticide residue often remains on the surface of cucumber fruits after application, but the metabolic pathway and genes involved in pesticide metabolism remain unclear. In this study, we employed whole transcriptional analysisusing a high-throughput tag-sequencing technique (Tag-seq) to identify the genes involved in cucumber’s metabolism of the fungicide propamocarb. Transcript abundance was investigated by analyzing gene expression profiles. Differential expression analysis revealed the up-regulation of 546 genes, and the down-regulation of 185 genes. Using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, genes identified from whole transcriptome analysis as related to the phenylpropanoid pathway were most significantly differentially expressed. Fourteen of these genes were up-regulated after treatment with propamocarb. Further analysis revealed that these genes encoded six enzymes: phenylalanine ammonia-lyase (PAL), cytochrome P450 (CYP), AMP-dependent CoA ligase (AMP), anthranilate Nbenzoyltransferase protein (ANP), UDP-glucosyl transferase family protein (UDP), and peroxidase (POD). Compared to the control, the activities of PAL and POD were significantly increased (p < 0.01) after treatment with propamocarb, as was lignin synthesis such that acetylbromide-extractable lignins were increased by 41.1% (p < 0.05). Based on these results, we propose that propamocarb up-regulates the expression of genes involved in the phenylpropanoid pathway, leading to induction of lignin synthesis, which in turn triggers defense mechanisms in cucumber.
Peng Fu,Zhi-Jun Xu,Ruiqing Chu,Wei Li,Qian Xie,Guo-Zhong Zang 한국물리학회 2011 Current Applied Physics Vol.11 No.3
Eu_2O_3 (0―0.8 wt.%)-doped 0.82Bi_0.5Na_0.5TiO_3―0.18Bi_0.5K_0.5TiO_3 (BNKT18) lead-free piezoelectric ceramics were synthesized by conventional solid-state processes. The compositional dependence of phase structure and electrical properties of the BNKT18 ceramics was studied. X-ray diffraction (XRD) data shows that 0.2―0.8 wt.% Eu_2O_3 has diffused into the lattice of BNKT18 ceramics and forms a pure perovskite phase. SEM images indicate that the grain size of BNKT18 ceramics decreases with the increase of Eu_2O_3 content, and all the modified ceramics have a clear grain boundary and a uniformly distributed grain size. At room temperature, the ferroelectric and piezoelectric properties of the BNKT18ceramics have been improved with the addition of Eu_2O_3, and the BNKT18 ceramics doped with 0.4 wt.%Eu_2O_3 have the highest piezoelectric constant (d_33 = 145 pC/N), highest relative dielectric constant (ε_r = 1134) and lower dissipation factor (tan δ = 0.045) at a frequency of 10 kHz. The BNKT18 ceramics doped with 0.2 wt.% Eu_2O_3 have the highest planar coupling factor (k_p = 0.251). The temperature dependence of the relative dielectric constant shows T_m increases with the addition of Eu_2O_3.
De Peng Yuan,Xiao Feng Xu,Hong Woo-Jong,Si Ting Wang,Xin Tong Jia,Yang Liu,Shuang Li,Zhi Min Li,Qian Sun,Qiong Mei,Shuai Li,정기홍,Song Hong Wei,Yuan Hu Xuan 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5
Sheath blight disease (ShB) severely afects rice production; however, the details of defense against ShB remain unclear. To understand the rice defense mechanism against ShB, an RNA sequencing analysis was performed using Rhizoctonia solani inoculated rice leaves after 48 h of inoculation. Among them, 3417 genes were upregulated and 2532 were downregulated when compared with the control group (>twofold or<1/2). In addition, the diferentially expressed genes were classifed via Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and MapMan analyses. Fifty-nine GO terms and seven KEGG pathways were signifcantly enriched. A MapMan analysis demonstrated that the phytohormone and metabolic pathways were signifcantly altered. Interestingly, the expression levels of 359 transcription factors, including WRKY, MYB, and NAC family members, as well as 239 transporter genes, including ABC, MFS, and SWEET, were signifcantly changed in response to R. solani AG1-IA inoculation. Additionally, OsWRKY53 and OsAKT1 negatively regulate the defense response in rice against R. solani via gain of function study for OsWRKY53 and loss of function study for OsAKT1, respectively. Furthermore, several diferentially expressed genes contain R. solani-responsive cis acting regulatory elements in their promoter regions. Taken together, our analyses provide valuable information for the additional study of the defense mechanisms against ShB, and the candidate genes identifed in this study will be useful resource for future breeding to enhance resistance against ShB.
( Guo Hua Yi ),( Zhi Min Wang ),( Yi Peng Qi ),( Lun Guang Yao ),( Juan Qian ),( Long Bo Hu ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.6
White spot disease (WSD) is caused by the white spot syndrome virus (WSSV), which results in devastating losses to the shrimp farming industry around the world. However, the mechanism of virus entry and spread into the shrimp cells is unknown. A binding assay in vitro demonstrated VP28-EGFP (envelope protein VP28 fused with enhanced green fluorescence protein) binding to shrimp cells. This provides direct evidence that VP28-EGFP can bind to shrimp cells at pH 6.0 within OS It However, the protein was observed to enter the cytoplasm 3 h post-adsorption. Meanwhile, the plaque inhibition test showed that the polyclonal antibody against VP28 (a major envelope protein of WSSV) could neutralize the WSSV and block an infection with the virus. The result of competition EI,ISA further confirmed that the envelope protein VP28 could compete with WSSV to bind to shrimp cells. Overall, VP28 of the WSSV can bind to shrimp cells as an attachment protein, and can help the virus enter the cytoplasm.
( Xiao Lan Liu ),( Xi Qun Zheng ),( Peng Zhi Qian ),( Narasimha Kumar Kopparapu ),( Yong Ping Deng ),( Masanori Nonaka ),( Naoki Harada ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.2
A fibrinolytic enzyme was produced by an edible mushroom of Pleurotus ostreatus using submerged culture fermentation. The enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. The enzyme was purified by a 147-fold, with a yield of 7.54%. The molecular masses of the enzyme an determined by gel filtration and SDSPAGE were 13.6 and 18.2 kDa, respectively. The isoelectric point of the enzyme was 8.52. It hydrolyzed fibrinogen by cleaving the α and β chains of fibrinogen followed by the γ chains, and also activated plasminogen into plasmin. The enzyme was optimally active at 45°C and pH 7.4. The enzyme activity was completely inhibited by EDTA, whereas protease inhibitors of TPCK, SBTI, PMSF, aprotinin and pepstatin showed no inhibition on its activity. The partial amino acid sequences of the enzyme as determined by Q-TOF2 were ATFVGCSATR, GGTLIHESSHFTR, and YTTWFGTFVTSR. These sequences showed a high degree of homology with those of metallo-endopeptidases from P. ostreatus and Armillaria mellea. The purified enzyme can also be applied as a natural agent for oral fibrinolytic therapy or prevention of thrombosis.
Contributed Mini Review : Isoforms, structures, and functions of versatile spectraplakin MACF1
( Li Fang Hu ),( Pei Hong Su ),( Run Zhi Li ),( Chong Yin ),( Yan Zhang ),( Peng Shang ),( Tuan Min Yang ),( Ai Rong Qian ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.1
Spectraplakins are crucially important communicators, linking cytoskeletal components to each other and cellular junctions. Microtubule actin crosslinking factor 1 (MACF1), also known as actin crosslinking family 7 (ACF7), is a member of the spectraplakin family. It is expressed in numerous tissues and cells as one extensively studied spectraplakin. MACF1 has several isoforms with unique structures and well-known function to be able to crosslink F-actin and microtubules. MACF1 is one versatile spectraplakin with various functions in cell processes, embryo development, tissue-specific functions, and human diseases. The importance of MACF1 has become more apparent in recent years. Here, we summarize the current knowledge on the presence and function of MACF1 and provide perspectives on future research of MACF1 based on our studies and others. [BMB Reports 2016; 49(1): 37-44]
Hao Tang,Nian-Guang Li,Zhi-Hao Shi,Yu-Ping Tang,Qian-Ping Shi,Ze-Xi Dong,Peng-Xuan Zhang,Jin-ao Duan 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10
The binding abilities of scutellarin (Scu) andscutellarein (Scue) with bovine serum albumin (BSA) wereinvestigated using equilibrium dialysis, high performanceliquid chromatography, fluorescence spectroscopy, competitivesite marker and molecular docking. The resultsshowed that the average protein binding ratios of Scu andScue with BSA were (79.85 ± 1.83) and (85.49 ± 1.21) %respectively. Under simulated physiological conditions, thefluorescence data indicated that Scu and Scue bound withBSA through a static mechanism. The thermodynamicparameters indicated that the interactions of Scu-BSA andScue-BSA mainly occurred by van der Waals forces andhydrogen bonds and it was easier for Scue to bind withBSA than Scu, indicating that the glucuronic acid moleculein Scu decreased the binding affinity. Site competitivemarker experiments showed that the binding sites of Scuand Scue mainly located within the sub-domain IIA ofBSA. Furthermore, molecular docking studies indicatedthat one BSA could bind three Scue, while one BSA couldcarry only two Scu. All these results clearly indicated theinteractions of Scu and Scue with BSA, which will lay thefoundation for further research to determine the pharmacologyand pharmacodynamics of Scu and Scue for treatingischemic cerebrovascular disease.