Cloning and Characterization of Squalene Synthase (SQS) Gene from Ganoderma Lucidum

( Ming Wen Zhao ),( Wan Qi Liang ),( Da Bing Zhang ),( Wang Nan ),( Chen Guang Wang ),( Ying Jie Pan ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.7

Analytical study on free vertical and torsional vibrations of two- and threepylon suspension bridges via d’Alembert’s principle

Wen-Ming Zhang,Zhi-wei Wang,Hao-qing Zhang,Xiao-fan Lu,Zhao Liu 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.76 No.3

This study derives the differential equations of free vertical bending and torsional vibrations for two- and three-pylon suspension bridges using d'Alembert's principle. The respective algorithms for natural vibration frequency and vibration mode are established through the separation of variables. In the case of the three-pylon suspension bridge, the effect of the along-bridge bending vibration of the middle pylon on the vertical bending vibration of the entire bridge is considered. The impact of torsional vibration of the middle pylon about the vertical axis on the torsional vibration of the entire bridge is also analyzed in detail. The feasibility of the proposed method is verified by two engineering examples. A comparative analysis of the results obtained via the proposed and more intricate finite element methods confirmed the former feasibility. Finally, the middle pylon stiffness effect on the vibration frequency of the three-pylon suspension bridge is discussed. It is found that the vibration frequencies of the first- and thirdorder vertical bending and torsional modes both increase with the middle pylon stiffness. However, the increase amplitudes of thirdorder bending and torsional modes are relatively small with the middle pylon stiffness increase. Moreover, the second-order bending and torsional frequencies do not change with the middle pylon stiffness.

Frequency-based tension assessment of an inclined cable with complex boundary conditions using the PSO algorithm

Wen-ming Zhang,Zhi-wei Wang,Dan-dian Feng,Zhao Liu 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.79 No.5

The frequency-based method is the most commonly used method for measuring cable tension. However, the calculation formulas for the conventional frequency-based method are generally based on the ideally hinged or fixed boundary conditions without a comprehensive consideration of the inclination angle, sag-extensibility, and flexural stiffness of cables, leading to a significant error in cable tension identification. This study aimed to propose a frequency-based method of cable tension identification considering the complex boundary conditions at the two ends of cables using the particle swarm optimization (PSO) algorithm. First, the refined stay cable model was established considering the inclination angle, flexural stiffness, and sag-extensibility, as well as the rotational constraint stiffness and lateral support stiffness for the unknown boundaries of cables. The vibration mode equation of the stay cable model was discretized and solved using the finite difference method. Then, a multiparameter identification method based on the PSO algorithm was proposed. This method was able to identify the tension, flexural stiffness, axial stiffness, boundary rotational constraint stiffness, and boundary lateral support stiffness according to the measured multiorder frequencies in a synchronous manner. The feasibility and accuracy of this method were validated through numerical cases. Finally, the proposed approach was applied to the tension identification of the anchor span strands of a suspension bridge (Jindong Bridge) in China. The results of cable tension identification using the proposed method and the existing methods discussed in previous studies were compared with the on-site pressure ring measurement results. The comparison showed that the proposed approach had a high accuracy in cable tension identification. Moreover, the synchronous identification of the flexural stiffness, axial stiffness, boundary rotational constraint stiffness, and boundary lateral support stiffness was highly beneficial in improving the results of cable tension identification.

Analytical methods for determining the cable configuration and construction parameters of a suspension bridge

Wen-Ming Zhang,Gen-min Tian,Chao-yu Yang,Zhao Liu 국제구조공학회 2019 Structural Engineering and Mechanics, An Int'l Jou Vol.71 No.6

Main cable configurations under final dead load and in the unloaded state and critical construction parameters (e.g. unstrained cable length, unstrained hanger lengths, and pre-offsets for tower saddles and splay saddles) are the core considerations in the design and construction control of a suspension bridge. For the purpose of accurate calculations, it is necessary to take into account the effects of cable strands over the anchor spans, arc-shaped saddle top, and tower top pre-uplift. In this paper, a method for calculating the cable configuration under final dead load over a main span, two side spans, and two anchor spans, coordinates of tangent points, and unstrained cable length are firstly developed using conditions for mechanical equilibrium and geometric relationships. Hanger tensile forces and unstrained hanger lengths are calculated by iteratively solving the equations governing hanger tensile forces and the cable configuration, which gives careful consideration to the effect of hanger weight. Next, equations for calculating the cable configuration in the unloaded state and pre-offsets of saddles are derived from the cable configuration under final dead load and the conditions for unstrained cable length to be conserved. The equations for the main span, two side spans and two anchor spans are then solved simultaneously. In the proposed methods, coupled nonlinear equations are solved by turning them into an unconstrained optimization problem, making the procedure simplified. The feasibility and validity of the proposed methods are demonstrated through a numerical example.

Crop Production and Environment friendly-agriculture in China

Ming Zhao,Lifeng Fang,Zhiqiang Dong,Xianguo Zhu,Wen Jiang 한국작물학회 2005 한국작물학회 학술발표대회 논문집 Vol.2005 No.-

Measurement of neutron cross sections and resonance parameters of ¹⁶⁹Tm below 100 eV

Wen-Ming, Wang,Xia, Li,Zhi-Xiang, Zhao,Zu-Ying, Zhou,Hong-Wei, Yu,Hai-Cheng, Wu,Yi-Xiang, Wei,Wang, T. F.,Kim, G. N.,Lee, M. W.,Kim, K. S.,Cho, M. H.,Ko, I. S.,Namkung, W. science press 2010 Chinese physics. C Vol.34 No.2

<P>The neutron total cross-sections of thulium (<SUP>169</SUP>Tm) were measured in the neutron energy region from 0.01 eV to 100 eV by using the time-of-flight method at the Pohang Neutron Facility, which consists of an electron linac, a water-cooled tantalum target with a water moderator, and a 12 m time of flight path. Two thulium plates with different thicknesses were used for the neutron transmission measurement. The background level was determined by using a notch-filter of Co, In, and Cd sheets. The present measurement was compared with the previous ones, and a new set of resonance parameters of <SUP>169</SUP>Tm isotope was obtained from the transmission rate by using the SAMMY code, with a comparison with the recommended parameters by Mughabghab.</P>

Analysis of Ferrous on Ten-Eleven Translocation Activity and Epigenetic Modifications of Early Mouse Embryos by Fluorescence Microscopy

Zhao, Ming-Hui,Liang, Shuang,Guo, Jing,Choi, Jeong-Woo,Kim, Nam-Hyung,Lu, Wen-Fa,Cui, Xiang-Shun Cambridge University Press 2016 Microscopy and Microanalysis Vol.22 No.2

<B>Abstract</B><P>Iron is an essential trace element that plays important roles in the cellular function of all organs and systems. However, the function of Fe(II) in mammalian embryo development is unknown. In this study, we investigated the role of Fe(II) during preimplantation embryo development. Depletion of Fe(II) using thiosemicarbazone-24 (TSC24), a specific Fe(II) chelator, rescued quenching of the Fe(II)-sensitive fluorophore phen green-SK. After <I>in vitro</I> fertilization, TSC24 significantly reduced the cleavage rate as well as blastocyst formation. The hatch rate of blastocysts was also reduced with 1 pM TSC24 treatment (20.25±1.86 versus 42.28±12.96%, <I>p</I><0.05). Blastocysts were cultured in leukemia inhibitory factor-free mouse embryonic stem cell culture medium with or without TSC24, and those with depleted Fe(II) displayed delayed attachment and lost the ability to induce embryoid body formation. To further explore the mechanism of Fe(II) in embryo development, we assessed the expression of 5-hydroxymethylcytosine (5hmC) and OCT4 in the pronuclear and blastocyst stages, respectively. We observed that Fe(II) reduced 5hmC and OCT4 expression, which could be explained by low ten-eleven translocation (TET) enzyme activity induced by TSC24 treatment. These findings demonstrate that Fe(II) is required for mammalian embryo development and that it facilitates the process via regulation of TET activity.</P>

Antioxidant Enzymes and Photosynthetic Responses to Drought Stress of Three Canna edulis Cultivars

Wen-E Zhang,Fei Wang,Xue-jun Pan,Zhi-guo Tian,Xiu-ming Zhao 한국원예학회 2013 원예과학기술지 Vol.31 No.6

Edible canna is a productive starch source in some tropical and semitropical regions. In these regions, water deficit stress is one of factors that limit the crop yield. In the present study, we investigated seven physiological indexes and photosynthetic responses of three edible canna (Canna edulis Ker.) cultivars (‘PLRF’, ‘Xingyu-1’, and ‘Xingyu-2’) under 35 days drought stress. Our results indicated that drought treatment caused visible wilting symptoms in all cultivars, especially in ‘Xingyu-1’. Coupled with the increase of wilting symptoms, relative water content (RWC) and chlorophyll content decreased progressively, malondialdehyde (MDA) content gradually increased, and key antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities increased first and then decreased in all three cultivars. The effect of water stress was more pronounced in ‘Xingyu-1’ than in ‘PLRF’ and ‘Xingyu-2’, and in lower leaves than in upper leaves. In addition, 35 days drought stress also significantly reduced the photosynthetic capacity. Consistent with antioxidant parameters, photosynthetic changes of ‘Xingyu-2’ were less than those of the other cultivars under water deficit stress. Drought stress caused a significant increase of water use efficiency (WUE) in ‘Xingyu-2’, but little in ‘PLRF’, and obvious decrease in ‘Xingyu-1’. These results indicated that ‘Xingyu-2’ was more tolerant to drought stress than ‘PLRF’ and ‘Xingyu-1’ by maintaining lower lipid peroxidation and higher antioxidant enzyme activities.

Antioxidant Enzymes and Photosynthetic Responses to Drought Stress of Three Canna edulis Cultivars

Zhang, Wen-E,Wang, Fei,Pan, Xue-Jun,Tian, Zhi-Guo,Zhao, Xiu-Ming Korean Society of Horticultural Science 2013 원예과학기술지 Vol.31 No.6

Edible canna is a productive starch source in some tropical and semitropical regions. In these regions, water deficit stress is one of factors that limit the crop yield. In the present study, we investigated seven physiological indexes and photosynthetic responses of three edible canna (Canna edulis Ker.) cultivars ('PLRF', 'Xingyu-1', and 'Xingyu-2') under 35 days drought stress. Our results indicated that drought treatment caused visible wilting symptoms in all cultivars, especially in 'Xingyu-1'. Coupled with the increase of wilting symptoms, relative water content (RWC) and chlorophyll content decreased progressively, malondialdehyde (MDA) content gradually increased, and key antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities increased first and then decreased in all three cultivars. The effect of water stress was more pronounced in 'Xingyu-1' than in 'PLRF' and 'Xingyu-2', and in lower leaves than in upper leaves. In addition, 35 days drought stress also significantly reduced the photosynthetic capacity. Consistent with antioxidant parameters, photosynthetic changes of 'Xingyu-2' were less than those of the other cultivars under water deficit stress. Drought stress caused a significant increase of water use efficiency (WUE) in 'Xingyu-2', but little in 'PLRF', and obvious decrease in 'Xingyu-1'. These results indicated that 'Xingyu-2' was more tolerant to drought stress than 'PLRF' and 'Xingyu-1' by maintaining lower lipid peroxidation and higher antioxidant enzyme activities.

Research on the Shared-network of SMV and GOOSE in Smart Substation

Wang, Wen-Long,Liu, Ming-Hui,Zhao, Xi-Cai 대한전기학회 2014 The Journal of International Council on Electrical Vol.4 No.2

The network structure of the smart substation in common use was introduced, and the technical problem of the shared-network of sampled measured value (SMV) and generic object oriented substation event (GOOSE) was analyzed, such as the processing ability of network device and the intelligent device, the data real-time property and the network reliability, the effects to the substation in the condition of network fault, and so on. On this basis, the feasibility of the shared-network of SMV and GOOSE was discussed, the implement scheme were presented, and eventually came to the solution of the shared-network of SMV and GOOSE, which based on the applications of the message priority control, restricting the switch number, virtual local area network (VLAN) and GARP multicast registration protocol (GMRP) classification flow control, etc. In the test-bed, the cases of shared-network and separate-network of SMV and GOOSE were compared and analyzed, and the result was valuable for reference.