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Fan Meiqi,Choi Young-Jin,Wedamulla Nishala Erandi,Bae Sung Mun,Kim Eun-Kyung 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.3
Larvae of Allomyrina dichotoma (rhinoceros beetle) were registered as a food in the Korean Food Standards Code. Interestingly, the ethanol or chloroform extracts of the larvae were reported to have some biological activities such as anti-inflammatory and hepato-protective. In the present study, an attempt was made to test anti-obesity activity of A. dichotoma larvae. For this purpose, the larvae were hydrolyzed with a proteinase, promod 278P, and the effects of this larval hydrolysate (ADL) on the diet-induced obese (DIO) mice were assessed in terms of changes of obesity-related parameters. DIO mice were prepared by feeding C57BL/6 mice with the high fat diet (HFD, 60% fat of its total calories) for 6 weeks, and 18 mice were selected as DIO mice that were 20% or more heavier than the control mice fed with the conventional diet (10% fat of total calories). The 18 DIO mice were further fed with the HFD for 5 weeks without ADL (HFD group), with ADL (200 mg/kg/day) (HFD + ADL group) or with orlistat (a positive control) (HFD + Orl group). Compared to the control group, HFD group showed the significant increase in fat tissue weight, levels of total cholesterol, low-density lipoprotein cholesterol and very low-density lipoprotein cholesterol as well as alanine aminotransferase, aspartate aminotransferase, hepatic accumulation of lipid droplets, and hepatic expressions of adipogenesis-stimulating proteins (peroxisome proliferator-activated receptor γ, CCAAT-enhancer-binding protein α, sterol regulatory element binding protein 1c, acetyl coenzyme A carboxylase and fatty acid synthase). All these increases were significantly lowered by ADL and orlistat. The effects of ADL on most of these parameters were greater than those of orlistat. These results show that the larvae of A. dichotoma has an anti-obesity activity and thus, may be used as a source for anti-obesity agents.
Meiqi Fan,Young-Jin Choi,Sung Mun Bae,Nishala Erandi Wedamulla,Eun-Kyung Kim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The aim of this study was to identify an anti-obesity peptide from Allomyrina dichotoma and investigate the mechanism. For that, enzymatically hydrolyzed A. dichotoma larvae were separated using tangential flow filtration and consecutive chromatographic processes. Finally, an anti-obesity peptide that showed the highest inhibitory effect on lipid accumulation was obtained, and the sequence was Glu-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu(EIA10). EIA10 decreased lipid aggregation in vitro and significantly reduced the accumulation of body weight gain, liver weight, and adipose tissue weight in high-fat-fed mice. Compared with the control group, the levels of TC, TG, LDL, insulin, and HOMA-IR in the HFD group increased significantly, and the content of HDL in the serum decreased significantly. On the contrary, the levels of TC, TG, and insulin in the EIA10 group decreased significantly, and the HDL content increased significantly compared with the HFD group. Additionally, EIA10 dramatically decreased mRNA and protein levels of transcription factors involved in lipid adipogenesis. Taken together, our results suggest that EIA10 could be a promising agent for the prevention of obesity.
Tang, Yujiao,Fan, Meiqi,Choi, Young-Jin,Choi, Eun-Ju,Moon, Sang-Ho,Debnath, Trishna,Yu, Yonghai,Lee, Il Nam,Kim, Eun-Kyung Hindawi Limited 2018 Journal of chemistry Vol.2018 No.-
<P>We previously discovered the antioxidant and antiprostate cancer effects of antler extract (AE), but whether it inhibits cisplatin- (Cis-) induced toxicity has not been investigated. In this study, the effect of AE on Cis-induced side effects in the kidney and liver using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide-based cytotoxicity and cell cycle assays in prostate cancer PC-3 cells in vitro is investigated. Furthermore, we used a xenograft mouse model of the same cells to examine the in vivo effects and mechanisms of action. Cis and Cis + AE treatment attenuated prostate cancer cell growth by inducing apoptosis in vitro. Cis + AE stimulated cleaved caspases 3, 7, and 9 and polyadenosine diphosphate ribose polymerase expression. Cis + AE treatment for 1 week significantly increased the superoxide dismutase and catalase antioxidant activity while thiobarbituric acid reactive substances decreased. The histopathological damage and tumor necrosis factor-α, interleukin- (IL-) 1<I>β</I> and IL-6, cyclooxygenase-2, and inducible nitric oxide synthase expression in the kidney and liver tissue decreased. Therefore, AE likely possesses antiprostate cancer activity and inhibits Cis toxicity.</P>
( Young-jin Choi ),( Meiqi Fan ),( Yujiao Tang ),( Masahiro Iwasa ),( Kwon-il Han ),( Hongchan Lee ),( Ji-young Hwang ),( Bokyung Lee ),( Eun-kyung Kim ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8
In the present study, we investigated the inhibitory effect of heat-killed Enterococcus faecalis (E. faecalis) and live E. faecalis on benign prostatic hyperplasia (BPH). The BPH rat model was established by administering male rats with testosterone propionate (TP, 5 mg/kg, in corn oil) via subcutaneous injections daily for four weeks after castration. The rats were divided into five groups: Con, corn oil-injected (s.c.) + DW administration; BPH, TP (5 mg/kg, s.c.) + DW administration; BPH+K_EF, TP (5 mg/kg, s.c.) + heat-killed E. faecalis (7.5 × 10<sup>12</sup> CFU/g, 2.21 mg/kg) administration; BPH+L_EF, TP (5 mg/kg, s.c.) + live E. faecalis (1 × 10<sup>11</sup> CFU/g, 166 mg/kg) administration; BPH+Fi, TP (5 mg/kg, s.c.) + finasteride (1 mg/kg) administration. In both of BPH+K_EF and BPH+L_EF groups, the prostate weight decreased and histological changes due to TP treatment recovered to the level of the Con group. Both of these groups also showed regulation of androgen-signaling factors, growth factors, and apoptosis-related factors in prostate tissue. E. faecalis exhibited an inhibitory effect on benign prostatic hyperplasia, and even heat-killed E. faecalis showed similar efficacy on the live cells in the BPH rat model. As the first investigation into the effect of heat-killed and live E. faecalis on BPH, our study suggests that heat-killed E. faecalis might be a food additive candidate for use in various foods, regardless of heat processing.
Choi Young-Jin,Fan Meiqi,Tang Yujiao,Moon Sangho,Lee Seung-Hyun,Lee Bokyung,Bae Sung Mun,Lee Sang Moo,Kim Eun-Kyung 한국영양학회 2022 Nutrition Research and Practice Vol.16 No.4
BACKGROUND/OBJECTIVES Benign prostatic hyperplasia (BPH) is the most common prostate disease and one of the most common chronic diseases caused by aging in men. On the other hand, there has been no research on BPH using Abeliophyllum distichum Nakai (A. distichum). Therefore, this study investigated the effects of A. distichum on BPH. MATERIALS/METHODS A. distichum leaves were extracted with distilled water, 70% ethanol, and 95% hexane as solvents. Subsequently, the inhibitory effects of each A. distichum extract on androgen receptor (AR) signaling were evaluated in vitro. The testosterone-induced BPH model was then used to confirm the efficacy of A. distichum leaves in 70% ethanol extract (ADLE). RESULTS ADLE had the strongest inhibitory effect on AR signaling. A comparison of the activity of ADLE by harvest time showed that the leaves of A. distichum harvested in autumn had a superior inhibitory effect on AR signaling to those harvested at other times. In the BPH rat model, the administration of ADLE reduced the prostate size and prostate epithelial cell thickness significantly and inhibited AR signaling. Subsequently, the administration of ADLE also reduced the expression of growth factors, thereby inactivating the PI3K/AKT pathway. CONCLUSIONS An analysis of the efficacy of ADLE to relieve BPH showed that the ethanol extract grown in autumn exhibited the highest inhibitory ability of the androgen-signaling related factors in vitro. ADLE also inhibited the expression of growth factors by inhibiting the expression of the androgen-signaling related factors in vivo. Overall, ADLE is proposed as a functional food that is effective in preventing BPH.