Effects of Negative Pressure Soft Tissue Therapy to Ankle Plantar Flexor on Muscle Tone, Muscle Stiffness, and Balance Ability in Patients with Stroke

Kim, Kyu Ryeong,Shin, Houng Soo,Lee, Sang Bin,Hwang, Hyun Sook,Shin, Hee Joon International Academy of Physical Therapy Research 2018 Journal of International Academy of Physical Ther Vol.9 No.2

The purpose of the study was to investigate the immediate effects of negative pressure soft tissue therapy on muscle tone, muscle stiffness and balance in patients with stroke. In total, 20 patients with stroke and assigned to the negative pressure soft tissue therapy group (NPST, n=10) or, placebo-negative pressure soft tissue therapy group(Placebo-NPST, n=10). Both groups underwent NPST or placebo-NPST once a day during the experimental period. MyotonPRO was used to assess the parameters for muscle tone and stiffness. Biorescue was used to assess the parameters for balance. Each group showed improvements in muscle tone, muscle stiffness, and balance ability (p<.05). Especially, Muscle tone, muscle stiffness, and anterior length in the limit of stability were the significant improvement on NPST group (p<.05). The results of the study suggest that the NPST is effective in improving muscle tone, muscle stiffness, and balance ability in patients with stroke.

Copper-Based Compounds against Erwinia amylovora: Response Parameter Analysis and Suppression of Fire Blight in Apple

Duck Kyu Ryu,Mahesh Adhikari,Dong Hyuk Choi,Kyung Jin Jun,Do Hyoung Kim,Chae Ryeong Kim,Min Kyu Kang,박덕환 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.1

Fire blight, caused by Erwinia amylovora, is one of the major bacterial disease of apple and pear, causing enormous economic losses worldwide. Several control measures against E. amylovora have been reported till date, however, none of them have proved to be effective significantly against the pathogen. In this study, mechanisms of the copper-based control agents (CBCAs): copper oxychloride (COCHL), copper oxide (COX), copper hydroxide (CHY), copper sulfate basic (CSB), and tribasic copper sulfate (TCS) and their disease severity reduction efficacy against E. amylovora were analyzed. Bis-1,3-dibutylbarbituric acid trimethine oxonol, carboxyl fluorescein diacetate succinimidyl ester, and 5-cyano-2,3-ditolyl tetrazolium chloride staining were used to check the damage of membrane potential, cytoplasmic pHin, and respiration of CBCAs-treated E. amylovora, respectively. High disturbance in the membrane potential of E. amylovora was found under COX and COCHL treatments. Similarly, higher significant changes in the inner cytoplasmic pHin were observed under COX, COCHL, and TCS treatment. CHY and COCHL-treated E. amylovora showed a significant reduction in respiration. In vitro bioassay results revealed that CHY, CSB, and TCS at 2,000 ppm reduced the severity of fire blight both in pre- and post-treatment of CBCAs in immature apple fruits and seedlings. Overall, the most effective CBCAs against E. amylovora could be CHY at 2,000 ppm as its showed inhibition mechanisms and disease severity reduction.

L-carnitine supplementation improves the cryosurvival and subsequent development of bovine embryos

Kyeong Yeob Kim,Youn Bae Park,Byeong Ho Kim,Jin Hee Lee,Ji Hye Lee,Chanuka Kulatunga,Dong Eon Kim,Kyu Hyun Kim,Ryeong Eun Kim,Yoon Seok Nam,Min Kyu Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Cryopreservation of bovine embryos is used to efficiently implant surrogate mothers. It has been widely accepted that high lipid content in the oocyte interrupts its survival during freeze-thaw cycles. Serum component in the culture medium is thought to increase the embryo`s lipid contents. Conversely, L-carnitine stimulates lipid metabolism by transporting long chain fatty acids into the mitochondria. Objective of this study was to analyze the effect of L-carnitine supplementation in IVM medium and defined IVC medium on the development, lipid contents and the cryosurvival of bovine IVF embryos. 0.0, 1.5, 3.0 and 6.0 mM L-carnitine was supplemented in IVM medium, respectively (IVM-LC 0.0, LC 1.5, LC 3.0 and LC 6.0). Development rate from the 2cell to the morula stages was higher in IVM-LC 3.0 groups than those of IVM-LC 6.0 (p<0.05). But there were no significant differences among the other groups in the blastocyst rates and lipid content results. When 0.0, 1.5, 3.0 and 6.0 mM L-carnitine were supplemented in IVC medium (IVC-LC 0.0, LC 1.5, LC 3.0 and LC 6.0), development competence was not significantly different between those embryos. Lipid contents of embryos treated L-carnitine (IVC-LC 1.5, 3.0 and 6.0) were significantly lower than embryos of non-treated group. L-carnitine was supplemented 0.0, 1.5, 3.0, 6.0 mM during IVM and 3.0 mM during IVC (LC 0.0 - 3.0, LC 1.5 – 3.0, LC 3.0 – 3.0, LC 6.0 – 3.0) and cryosurvival of blastocysts confirmed after freezing-thawing. There were no significant differences on development, but LC 3.0 – 3.0 was significantly lower lipid contents than other groups. And LC 3.0 – 3.0 had better survival rates and hatched rates of blastocysts than LC 0.0 – 0.0. In conclusion, supplementation of L-carnitine in defined IVC medium decreases lipid contents. And L-carnitine supplementation improves cryosurvival and developmental ability of bovine IVF embryos.

경상북도 거주 노인의 정신건강과 자살 위험인자에 관한 연구

김정령(Jeong Ryeong Kim),이광헌(Kwang Hun Lee),김상규(Sang Kyu Kim),곽경필(Kyung Phil Kwak),사공정규(Jeong Kyu Sakong) 대한생물치료정신의학회 2021 생물치료정신의학 Vol.27 No.3

Objectives:The purpose of this study was to identify suicide risk factors among elderly residents in Gyeongsangbuk-do and to reveal the relationships between them. In addition, the differences in suicide risk factors were analyzed between the cognitively impaired group and the cognitively normal group. Methods:We investigated 20,127 elderly over 65 years of age, from January 2019 to December 2019. Participants were asked to complete a questionnaire survey. Cognitive function, depression, anxiety, sleep disturbance, and suicidal ideation data were assessed using a Korean version of the Mini-Mental Status Examination for Dementia Screening (MMSE-DS), Short Geriatric Depression Scale for Korean version (S-GDS), Geriatric Anxiety Inventory (GAI), Athens Insomnia Scale (AIS), and Scale for Suicidal Ideation (SSI). Data were analyzed using the chi-squared test and logistic regression analysis to examine suicide risk factors and the relationships between them. Furthermore, differences in suicide risk factors according to cognitive function were examined. Results:Age, cognitive function, depression, anxiety, and sleep disturbance were identified as suicide risk factors among elderly residents in Gyeongsangbuk-do. Depression was the factor that increased risk of suicide the most, followed by anxiety, impaired cognitive function, sleep disturbance, and 65-74 years of age. In addition, depression increased risk of suicide by 1.86 times in the cognitively impaired group. Conclusions:Among elderly residents in Gyeongsangbuk-do, depression was the factor that contributed the highest risk for suicide. More active prevention and treatment measures for depressive symptoms should be implemented in the cognitively impaired group.

보건의료기관 학습공동체 활동의 커뮤니케이션 특성과 조직 업무성과 간의 연관성: K기관 사례를 중심으로

김원령 ( Won Ryeong Kim ),박지연 ( Ji Yeon Park ),정규일 ( Kyu Ill Jong ),김혜경 ( Hye Kyeong Kim ),이은희 ( Eun Hee Lee ),김성대 ( Sung Dae Kim ),조한익 ( Han Ik Cho ) 대한보건협회 2013 대한보건연구 Vol.39 No.2

Objectives: The object of this study was to identify the relationship between communication characteristics of Communities of Practice(CoP) and organizational performance outcomes. Methods: A cross-sectional study was used and targeted 1,133 employees of K public health and medical institution who participated in CoP activities in 2012. Data on individual and communication characteristics of CoP activities, and individual and organizational outcomes were collected through self-administered questionnaires. The collected data were statistically analyzed by SPSS 18.0(K) Program. Results: here were significant differences among 10 communication characteristics of CoP depending on gender, duration of work and study hours(p<.001). Male workers were more likely to show individual and organizational outcomes from CoP than female workers. Both longer duration of work and time for CoP activities were positively related to individual and organizational outcomes. Linear regression analysis to understand the relationship between communication quality of CoP and individual performance, and organizational outcomes showed that ß of communication quality was 0.337(p<.001) and that of individual performances was 0.605(p<.001). Thus it was confirmed that two factors were significant factors on the organizational performance outcomes. The accountability of the regression model was 83.8%. Conclusion: The CoP participation had a positive effect on the communication quality, and individual and organizational performance outcomes. So, CoP could be the key strategy for facilitating evidence-based practice in public health and medical settings.

Neuronal over-expression of human Alzheimer's disease related genes in canines

Chanuka Kulatunga,Dong Eon Kim,JI Hye Lee,Kuk Bin Ji,Eun Ji Lee,Kyeong Yeob Kim,Beom Sik Kim,Kyu Hyun Kim,Ryeong Eun Kim,Yoon Seok Nam,Min Kyu Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

The early-onset familial Alzheimer's disease (EOFAD/ FAD), the less common type of Alzheimer's disease (AD) currently affects a vast number of individuals worldwide. This type is being inherited as an autosomal dominant fashion. Missense mutations on Amyloid precursor protein (APP) and Presenilins 1 and 2 (PSEN1 & PSEN2) are known as major genetic factors in FAD. Conversely, missense mutations on microtubule-associated protein tau (MAPT) are also thought to involve. Up to date, several triple-transgenic animal models with muted forms of the human APP, PSENs and MAPT have been reported. Compared to other animals, canines are more emotional and their disease signs can be easily diagnosed. This attempt was to develop a triple transgenic canine model for the AD. We have obtained the coding sequences of APP, PSEN1 and MAPT from Dana-Farber/Harvard Cancer Center DNA resource core at HMS and incorporated several common AD mutations. The transgenic construct is composed of hNSE (ENO2) promoter-driven three AD genes fused together with modified 2A sequences. It was transfected into the canine fetal fibroblasts which were then used to perform somatic cell nuclear transfer (SCNT). The viable transgenic embryos were obtained after in vitro culture and the GFP was detected. In this study, we have successfully produced viable triple transgenic canine cloned embryos using SCNT technique. These transgenic canine embryos will be further developed into canines with FAD. The transgenic canines will be a good candidate in the AD research field.

Investigation of the Potential of Modified Handmade Cloning (mHMC) Assisted with Demecolcine (DEM) in Porcine Embryo Cloning

Eun Ji Lee,Kuk Bin Ji,Kang Sun Park,Kyeong Yeob Kim,Beom Sik Kim,Kyu hyun Kim,Ryeong Eun Kim,Eun Young Kim,Ji Hye Lee,Ju Lan Chun,Min Kyu Kim 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06

Somatic cell nuclear transfer(SCNT) using micromanipulator have been used as a traditional cloning technique and applied various researches during the last decades. However the micromanipulator is expensive and relatively longer training period is required to operate it efficiently. Handmade cloning(HMC) is an alternative cloning method in a simplified way compared to the traditional cloning. Here, we suggested modified porcine handmade cloning(mHMC) as a new approach to clone porcine embryos as a substitute of the traditional SCNT. In mHMC, a nucleus was removed by an aspiration method by using a glass pipette, instead of bisection method. In this study, we investigated the efficiency of the enucleation method in mHMC by assessing the developmental competence of embryos in comparison with the traditional SCNT. The efficiency of enucleation was evaluated based on the rate of the accuracy and oocyte survivability. The accuracy of enucleation was lower in mHMC compared to those in SCNT(98.01±0.57 vs. 83.83±2.47), and the rate of survived oocytes was also lower in mHMC(96.50±0.84 vs. 90.10±2.11, respectively). And the developmental competence was assessed. The blastocyst rate was significantly higher in mHMC group(13.53±2.08 vs. 20.48±0.99). The levels of apoptosis and ROS were investigated to evaluate embryo quality. The expression of ROS and apoptosis-related genes showed no difference between groups. And the relative expressions of mRNA of pluripotency genes and reprogramming genes were evaluated. Although DNMT1 and DNMT3α were not differently expressed in two groups, the expression of the one of pluripotent gene, Oct4 was significantly higher in mHMC. In conclusion, based on the comparable results of mMHC and SCNT, the mHMC could be a suitable alternative technique to clone embryos in cost effective way compared to traditional SCNT.

방사선치료를 위한 CT 검사 시 3DCT와 4DCT에 대한 피폭선량 고찰

박령황(Ryeong-Hwang Park),김민정(Min-Jung Kim),이상규(Sang-Kyu Lee),박광우(Kwang-Woo Park),전병철(Byeong-Cheol Jeon),조정희(Jeong-Hee Cho),유병규(Beong-Gyu Yoo),이종석(Jong-Seok Lee) 대한방사선과학회(구 대한방사선기술학회) 2011 방사선기술과학 Vol.34 No.4

방사선치료를 위한 CT 검사 시 동일 환자에 대하여 3차원영상과, 호흡주기영상을 획득하기 위한 컴퓨터단층촬영에서 환자의 피폭선량을 측정하고자 SOMATON SENSATION OPEN(SIEMENS, GERMANY)을 이용하여 내원환자 중 폐암환자 10명, 간암환자 10명의 CT 검사 시 피폭선량을 측정했다. 환자가 받는 피폭선량은 The volume CT dose index(CTDIvol), Dose Length Product(DLP)를 이용하여 분석하였으며 각각의 장기들이 받는 피폭선량의 실측은 환자의 장기를 대상으로 할 수 없어 Rando 팬텀을 이용 흉부검사 시 폐와 심장, 척수를, 복부검사 시 간과 신장의 위치를 선택하여 in-vitro와 in-vivo 계측이 가능한 광유도발광선량계(Optically Stimulated Luminescent Dosimeter, Landauer, Inc., USA)를 이용하여 측정하였다. 폐암환자의 CT 검사 시 10명의 CTDIvol값은 5.7배, DLP값은 약 2.4배, 간암환자의 CTDIvol값은 3.8배, DLP값은 약 1.6배의 값을 나타내었고, OSLD를 이용한 실측정치 역시 폐암환자의 경우 6배, 간암환자의 경우 5.5배의 차이를 보이는 등 4DCT 검사에서 전체적인 피폭선량의 증가를 볼 수 있었다. 방사선치료 시 호흡에 의한 치료부위의 위치변화를 4DCT 검사를 이용하여 움직임을 보정하여 치료계획시 치료용적의 정확성을 높일 수 있으나 4DCT 검사로 인한 환자의 피폭선량 증가를 고려하여 검사시간과 검사범위를 줄여 피폭선량을 감소시키기 위한 노력이 필요하다. This study was to measure the patient dose difference between 3D treatment planning CT and 4D respiratory gating CT. Study was performed with each 10 patients who have lung and liver cancer for measured patient exposure dose by using SOMATON SENSATION OPEN(SIMENS ,GERMANY). CTDIvol and DLP value was used to analyze patient dose, and actual dose was measured in the location of liver and kidney for abdominal examination and lung, heart and spinal cord for chest examination. Rando phantom were used for the experiment. OSLD was used for in-vitro and in-vivo dosimetry. Increasing overall actual dose in 4D respiratory gated CT-simulation using OSLD increase the dose by 5.5 times for liver cancer patients and 6 times for lung cancer patients. In CT simulation of 10 lung cancer patients, CTDIvol value was increased by 5.7 times and DLP 2.4 times. For liver cancer patients, CTDIvol was risen by 3.8 times and DLP 1.6 times. The accuracy of treatment volume could be increased in 4D CT planning for position change due to the breaths of patient in the radiation therapy. However, patients dose was increased in 4D CT than 3D CT. In conclusion, constant efforts is required to reduce patients dose by reducing scan time and scan range.

Prognostic Signifi cance of the Lymph Node Ratio Regarding Recurrence and Survival in Rectal Cancer Patients Treated with Postoperative Chemoradiotherapy

( Ji Yoon Kim ),( Su Mi Chung ),( Byung Ock Choi ),( In Kyu Lee ),( Chang Hyeok An ),( Jong Man Won ),( Mi Ryeong Ryu ) 대한소화기학회 2012 Gut and Liver Vol.6 No.2

Background/Aims: To evaluate the prognostic impact of the lymph node ratio (LNR: the ratio of positive lymph nodes to the total number of lymph nodes examined) on disease recurrence and survival among rectal cancer patients who received curative surgery and postoperative chemoradiotherapy (CRT). Methods: Between 1995 and 2008, 124 patients with pathologic T3-4 or node-positive rectal cancer underwent curative surgery and postoperative CRT. Postoperative radiotherapy was delivered at a median dose of 50.4 Gy (range, 45 to 59.4 Gy) for 6 weeks. Chemotherapy consisted of a bolus injection of 5-fl uorouracil and leucovorin in the first and last week of radiotherapy (91.9%) or daily capecitabine during radiotherapy (8.1%). Further adjuvant chemotherapy was administered after chemoradiation. Results: The median follow-up was 5.1 years. In the multivariate analysis, pathologic N (pN) stage and lymphovascular invasion were signifi cantly associated with disease-free survival and disease-specific survival (p<0.05). However, when the LNR with a cutoff value of 0.2 was included as a covariate in the model, the LNR was highly signifi cant (p<0.001), and the pN stage lost its signifi cance (p>0.05). Conclusions: The LNR predicts recurrence and survival more accurately than pN stage. The pN stage and the LNR should be considered together when estimating the risk of disease recurrence among rectal cancer patients. (Gut Liver 2012;6:203-209)

플로팅 기술을 이용한 VR 광학계 설계

안아령(A-Ryeong An),최진혁(Jin-Hyeok Choi),박철우(Chul-Woo Park),김지원(Ji-Won Kim),이정현(Jeong-Hyeon Lee),김규호(Kyu-Ho Kim),오정훈(Jeong-Hoon Oh),정미숙(Mee-Suk Jung) 한국조명·전기설비학회 2021 한국조명·전기설비학회 학술대회논문집 Vol.2021 No.5