Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

축산물 수요모형의 비교와 선정

김태균,박계원,성명환 韓國農業政策學會 1999 농업경영정책연구 Vol.26 No.2

AR기법을 이용한 SLAM 영상 개선

김현,송대건,황기환,연규헌,전계석 慶熙大學校 大學院 1998 高凰論集 Vol.22 No.-

In this paper, we have been studied the enhancement of SLAM image using average response methos. AR methos has been used to reduce the random noise in signal processing. In this the experiment, we have constructed the SLAM system of 10㎒ operating frequency and experienced for holed Al sample. In this experimental result, we have been found that SLAM using AR method was able to get the high-speed and enhanced image compared with SAM.

재활용 플라스틱의 활용성 향상을 위한 디자인 개선에 관한 연구 : PET용기를 중심으로

이계환,김철복 한국디자인과학학회 1999 디자인과학연구 Vol.3 No.-

인류가 도구를 사용하면서부터 자연환경에서 자원을 채취하고 폐기물을 자연 생태계로 배출함으로써 현재와 같은 고도의 문명사회를 구축해 왔다. 자연생태계에는 순환시스템이 있어 인간활동의 규모가 별로 크지 않고 그 폐기물이 적을 경우, 그것은 자연생태계의 순환시스템 안에서 분해되고 다시 자원으로 전환되어 그 영향을 그다지 크지 않았다. 그러나 인류의 사회경제활동이 확대되고 자원 채취량이 자연재생 능력의 범위를 넘고, 또 폐기물량이 자연 생태계에서 수용할 수 있는 범위를 초과해 버리면 자원고갈이나 생태계의 파괴, 폐기물배출로 인한 오염물질 축적이라는 환경문제가 발생하여 지구의 유한성을 인식하게 되었다. 이미 지구상에는 사용하다가 버리는 이 물질문명을 앞으로도 유지해 나갈 수 있을 정도로 방대한 자원량과 무한한 환경이 존재하지 않는 것으로 확실해졌다. 한편, 오늘날의 환경문제는 규모면에서 오존층 파괴나 열대림 감소 등 지구 전체적 규모로써 그 대상이 되는 문제도 이제까지의 대기오염, 수질오염, 소음이라는 국부적 문제뿐만 아니라 지구 온난화 문제나 고형폐기물 문제 등으로 확대되어 가고 있다. 이 같은 지구환경의 용량한계 때문에 21세기 중에도 지구 생명권이 중대한 위기를 맞이하지 않을 수 없는 상황에 있다. 이러한 시점에서 본 연구에서는 현재 인류의 생존에 직접적으로 영향을 미치고 있는 환경오염에 관해 적극적으로 대체할 수 있는 디자인 측면에서의 해결 방안을 찾고자 한다. Since after Human Doing had began to use the instruments, the people have collected the resources out of natural environment and discharged waste products to the natural ecology, then, have built up highly civilized society like the present time. As you are aware of. there is a circulating system in the natural ecology, and if the scale of human activities are not so large enough, and the waste products are less in quantity, they are dissolved and reduced again into resources, within the range of circullating system of the natural ecology. and no serious influences have been arised. However, as social and economical activities of the human being are expanded, as volume of resources collection outstrips the scope of ability of the natural regeneration, and as the volume of waste products overruns the ability of the natural ecology, deposit of substances contaminated by exhausted waste products are being risen, and it evokes there cognition of threshold of the globe. It is certain that there do not exist enormous volume of resources and infinite environment on this 131o0e to enable us to continue enjoying such material civilization as people use the things and throw them away. On the other hand, current environmental issues disclose the whole global problems such as destruction of ozone layer and decrease of tropical forest, and their object are being spreader out to greenhouse effect of the globe, solid waste products, etc. As well as air pollution, water con contamination and noise that we have had so far. We may say this has been caused by the fact that both Quantity and quality of waste products produced by human being exceeded the range of natural circulation, and it my be inevitable to encounter with critical danger on our global environment. This study is intended to seek the design-oriented solution methods to cope actively with the environmental contamination that Inflicts direct impact on the existence of the human being nowadays.

자궁선근증의 고찰

조환성,이해혁,정성윤,김성욱,이순곤,남계현,이임순,이권해 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Objective: This study was designed to estimate the frequency and risk factor for edenomyosis Methods: The clinical records of 623 women undergoing hysterectomy were retrieved in the Department of Obstetrics and Gynecology, Soonchunhyang University Hospital, during 5 years, from Jan. 1995 to Dec. 1999. Results: The following result were obtained. 1. Adenomyosis was found in 247 of 623 patients(39.6%) 2. The highest incidence was 40-49 years of age group, an incidence of 46.6% and mean age was 44.7 years. 3. Adenomyosis was more frequently observed in parous women than non-parous women, such as 93.1% and 6.9%, respectively. 4. Dysmenorrhea, metrorrhagia, pelvic pain and vaginal bleeding were common symptom of adenomyosis, an incidence of 28.7%, 19.0%, 18.2% and 16.2%, respectively. 5. Myoma was the most combined disease in adenomyosis, showing the incidence of 54.7%. 6. Grossly, the size of uterus was enlarged more than 10 weeks gestational size in adenomyosis, an incidence of 61.9%. 7. Preoperative diagnostic accuracy of adenomyosis was 6.9%. Conclusion: The results show that deeply understanding of the common symptoms and epidemiology of adenomyosis improve the preoperative diagnostic accuracy.

조직확장술및 장골이식을 이용한 하악골 결손부의 재건

이계영,민경인,정수일,박재범,안재진,김도균,김철환 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

Soft tissue expansion is widely used technique in oral & maxillofacial reconstruction and provide new method of reconstruction in posttraumatic alopecia, post burn, wide scar, congenital deformity, benign tumor, tattoo, etc. Expanded tissue flaps have the advantage of increased vascularity, proximity to the defect, and similarity of color and texture. They also preclude the need to advance flaps from distant sites. Tissue expansion can be used to form a well vascularized cavity to accomodate and nourish bone grafts. The following report describes the uses of tissue expanders by allowing bone grafting to correct both soft and bony defects of mandibular region

유방초음파 검사의 유용성에 대한 연구

문양인,김병학,명화련,도윤수,김계환,손정훈,김미회 한국건강관리협회 2000 건협 Report Vol.3 No.-

톡소포자충 배양시 약제투여에 따른 Toxoplasma P30 유전자의 발현

이상걸,이영하,김계영,나영언,신대환 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

To know the drugs effect on gene expression and its regulating mechanism by RT-PCR and southern blot hybridization assay based on detection of the P30 gene encoding the major surface antigen, P30 of Toxoplasma gondii and the DNA synthesis in T. gondii infected HeLa cells. The experiment was performed and the results were as follows. 1. The thymidine incooperation in DNA of HeLa cell and T. gondii infected HeLa cell reached peak level at 36 hours and suddenly decreased at 48 hours. The uracil incooperation in DNA of T. gondii reached peak level at 48 hours. 2. The decrease of P30 gene expression began from 24 hours after pyrimethamine treated and 36 hours after methotrexate treated in T. gondii. The results were some what different. Pyrimethamine was more sensitive and acted earlier to T. gondii than Methotrexate did. The decrease of P30 gene expression of T. gondii was regulated these drugs in dose dependent manner. 3. The effects of dibutyryl cyclic AMP on P30 gene expression increased peak at 24 hours and on contrary decreased at 60 hours after treatment in dose dependent manner, but at 10mM high concentration of dibutyryl cAMP, P30 gene expression was supressed. 4. Actinomycine D supressed the P30 gene expression of T. gondii in HeLa cell. As the results, we presume pyrimethamine acts more powerful and earlier to supresses P30 gene expression than methotrexate, and it is regulated at transcription level. The results of RT-PCR were in agreement with that of southern blot hybridization.

슬관절 골관절염 증상이 환자에게 미치는 우울 효과

박영규,이윤영,이계우,김정아,윤도경,박승회,조경환,홍명호,박용규 대한노인병학회 2001 대한노인병학회지 Vol.5 No.4

국내 하수오니에 대한 퇴비원료로의 활용성 검토

권순익,임동규,이상범,김계훈,고문환 한국환경농학회 2003 한국환경농학회지 Vol.22 No.2

국내에서 발생되는 하수오니에 대한 퇴비원료로의 활용성을 검토하기 위하여 전국 150개소의 하수처리장 중에서 82개소로부터 88점의 하수오니를 채취하여 중금속 함량을 분석하였다. 하수오니의 구분은 하수처리장이 위치한 지역의 인구를 기준으로 광역시(100만명 이상), 대도시(50만~100만명), 중소도시(10만~50만명) 및 농총지역(인구 10만명 이하 소도시 포함)으로 구분하여 몇 가지 중금속을 분석하고 현재의 퇴비원료 기준과 비교 검토하였다. 성분별 전국적인 분포는 As 0.0~54.3 mg/kg, Cd 1.7~197.3 mg/kg, Cr 7.3~2,854 mg/kg, Cu 87.4~5,730 mg/kg, Mn 129.3~10,289 mg/kg, Ni 20.1~2,057 mg/kg, Pb 7.6~197.5 mg/kg, Zn 523.5~6,349 mg/kg의 범위에 있었다. 퇴비원료기준과 비교하였을 때 As, Cd, Cr, Cu, Ni, Pb 및 Zn 중 어느 한 성분이라도 함량이 초과하는 비율은 광역시 및 대도시는 100%, 중소도시 95%, 농촌지역 93%로 나타났다. 중금속 성분별로는 전체시료 중 As는 1%, Cd 41%, Cr 15%, Cu 53%, Ni 47%, Pb 2% 및 Zn은 89%가 기준치를 초과하였다. The objective of this experiment was to investigate the possibility on agricultural use of municipal sewage sludge with respect to heavy metal contents in Korea. Metal contents (mg/kg) in 88 sludge samples were ranged from 0 to 54.3 for As, 1.7 to 197.3 for Cd, 7.3 to 2,854 for Cr, 87.4 to 5,730 for Cu, 129.3 to 10,289 for Mn, 20.1 to 2,057 for Ni, 7.6 to 197.5 for Pb, and 523.5 to 6,349 for Zn. Heavy metal contents of sludges were compared with the regulation on raw material for compost. All sludges produced from sewage treatment plants in metropolis (over a million in population) and large cities (500,000∼l,000,000) were not compatible with the regulation. In addition, 95% of sludge from small and mid-size cities (100,000∼500,000) and 93% from rural area (below l00,000) were not suitable. On the basis of Cd, Cu, Ni, and Zn contents, 41, 53, 47, and 89% of sludge samples were not compatible with the regulation on raw material for compost, respectively.