SARS-CoV-2 infection engenders heterogeneous ribonucleoprotein interactions to impede translation elongation in the lungs

Kim Junsoo,Youn Daehwa,Choi Seunghoon,Lee Youn Woo,Sumberzul Dulguun,Yoon Jeongeun,Lee Hanju,Bae Jong Woo,Noh Hyuna,On Dain,Hong Seung-Min,An Se-Hee,Jang Hui Jeong,Kim Seo Yeon,Kim Young Been,Hwang Ji 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Translational regulation in tissue environments during in vivo viral pathogenesis has rarely been studied due to the lack of translatomes from virus-infected tissues, although a series of translatome studies using in vitro cultured cells with viral infection have been reported. In this study, we exploited tissue-optimized ribosome profiling (Ribo-seq) and severe-COVID-19 model mice to establish the first temporal translation profiles of virus and host genes in the lungs during SARS-CoV-2 pathogenesis. Our datasets revealed not only previously unknown targets of translation regulation in infected tissues but also hitherto unreported molecular signatures that contribute to tissue pathology after SARS-CoV-2 infection. Specifically, we observed gradual increases in pseudoribosomal ribonucleoprotein (RNP) interactions that partially overlapped the trails of ribosomes, being likely involved in impeding translation elongation. Contemporaneously developed ribosome heterogeneity with predominantly dysregulated 5 S rRNP association supported the malfunction of elongating ribosomes. Analyses of canonical Ribo-seq reads (ribosome footprints) highlighted two obstructive characteristics to host gene expression: ribosome stalling on codons within transmembrane domain-coding regions and compromised translation of immunity- and metabolism-related genes with upregulated transcription. Our findings collectively demonstrate that the abrogation of translation integrity may be one of the most critical factors contributing to pathogenesis after SARS-CoV-2 infection of tissues.

유무인복합기의 충돌회피를 위한 지상모니터링장비 개발

소준수(Junsoo So),배진근(Jinkeun Bae),신이섭(Leeseop Shin),현정욱(Jungwook Hyun),진상현(Sanghyun Jin),이정은(Jeongeun Lee) 항공우주시스템공학회 2018 항공우주시스템공학회 학술대회 발표집 Vol.2018 No.11

A novel approach to nucleic acid extraction using cross-linker conjugated magnetic beads

Minju Bae(배민주),HyeonAh Seong(성현아),Junsoo Park(박준수),Sehyun Shin(신세현) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.4

Analysis of Carbonization Behavior of Hydrochar Produced by Hydrothermal Carbonization of Lignin and Development of a Prediction Model for Carbonization Degree Using Near-Infrared Spectroscopy(열수 탄화 공정을 거친 리그닌 하이드로차(hydrochar)의 탄화 거동 분석과 근적외선 분광법을 이용한 예측 모델 개발)

( Un Taek Hwang ),( Junsoo Bae ),( Taekyeong Lee ),( Sung-yun Hwang ),( Jong-chan Kim ),( Jinseok Park ),( In-gyu Choi ),( Hyo Won Kwak ),( Sung-wook Hwang ),( Hwanmyeong Yeo ) 한국목재공학회 2021 목재공학 Vol.49 No.3

In this paper, we investigated the carbonization characteristics of lignin hydrochar prepared by hydrothermal carbonization and established a model for predicting the carbonization degree using near-infrared spectroscopy and partial least squares regression. The carbon content of the hydrothermally carbonized lignin at the temperature of 200 ℃ was higher by approximately 3 wt% than that of the untreated sample, and the carbon content tended to gradually increase as the heating time increased. Hydrothermal carbonization made lignin more carbon-intensive and more homogeneous by eliminating the microparticles. The discriminant and predictive models using near-infrared spectroscopy and partial least squares regression approppriately determined whether hydrothermal carbonization has been applied and predicted the carbon content of hydrothermal carbonized lignin with high accuracy. In this study, we confirmed that we can quickly and nondestructively predict the carbonization characteristics of lignin hydrochar manufactured by hydrothermal carbonization using a partial least squares regression model combined with near-infrared spectroscopy. 본 논문에서는 열수 탄화(hydrothermal carbonization)에 의해 제조된 리그닌 하이드로차의 탄화 특성을 조사하였고, 근적외선 분광법과 부분 최소 제곱(partial least squares) 회귀를 이용하여 탄화 거동을 예측하기 위한 모델을 수립하였다. 온도 200℃에서 열수 탄화된 리그닌의 탄소 함량은 무처리 시료 보다 약 3 wt% 높았으며 가열 시간이 증가할수록 탄소 함량도 서서히 증가하는 경향이 나타났다. 열수 탄화는 리그닌을 더욱 탄소 집약적으로 변화시키고 마이크로 파티클을 제거하여 더욱 균질한 특성을 부여하였다. 근적외선 분광법과 부분 최소 제곱 회귀를 이용한 판별 및 예측 모델은 수열 탄화의 적용 여부를 완벽히 구분했으며 높은 정확도로 열수 탄화 리그닌의 탄소 함량을 예측하였다. 본 연구로부터 근적외선 분광법과 결합된 부분 최소 제곱 회귀 모델을 이용하여 열수 탄화에 의해 제조된 리그닌 하이드로차의 탄화 특성을 빠르고 비파괴적으로 예측할 수 있다는 것이 확인되었다.

애니로직 시뮬레이션을 이용한 무인지상차량 운용성능과 전투효과의 연관성 분석

이재영(Jaeyeong Lee),신선우(Sunwoo Shin),김준수(Junsoo Kim),배성민(Sungmin Bae),김종만(Chongman Kim) 한국신뢰성학회 2015 신뢰성응용연구 Vol.15 No.2

In modern warfare, the number of unmanned systems grow faster than any other weapon systems. Therefore, it is very important to predict and measure the combat effectiveness (CE) of unmanned weapon systems in battlefield for deciding defense budget to acquire those systems. In general, quantitative calculation of weapon effectiveness under complicated battlefield is difficult based on the future network centric warfare. Hence, many papers studied how to measure the combat effectiveness and tried to study a lot of related issues about it. However, there are few papers dealing with the relationship between the UGV (Unmanned Ground Vehicle)’s performance and CE in a ground battlefield. In this paper, we do the sensitivity analysis based on a given scenario in a small unit battle. In order to do that, we developed simulation model using AnyLogic and changed the input parameters such as detection and hitting probabilities. We also assess the simulation outputs according to the variation of input parameters. The MOE used in this simulation model output is survival ratio for Blue force. We hope that this paper will be useful to find which input variable is more effective to increase combat effectiveness in a small unit ground battlefield.

근적외선 분광분석법을 이용한 열수탄화 강제냉각 공정을 거친 리그닌 바이오챠 분석

황운택 ( Untaek Hwang ),김현빈 ( Hyunbin Kim ),배준수 ( Junsoo Bae ),황성윤 ( Sungyun Hwang ),최종규 ( Jonggyu Choi ),박용건 ( Yonggun Park ),최인규 ( Ingyu Choi ),곽효원 ( Hyowon Kwak ),황성욱 ( Sungwook Hwang ),여환명 ( Hwanmyeo 한국목재공학회 2020 한국목재공학회 학술발표논문집 Vol.2020 No.1

열수탄화 후 강제냉각 공정을 통해 크라프트 리그닌 바이오챠를 제조하고 근적외선(Near Infrared ray, NIR)을 이용하여 분석하였다. 크라프트 리그닌을 증류수 140ml와 각각 2.8g, 5.6g, 8.4g(질량비 1:50, 2:50, 3:50)으로 혼합하여 가열 용기에서 200°C로 1시간, 2시간, 3시간, 5시간, 10시간 동안 가열하였다. 반응 과정에서는 온도와 전력량을 시간에 따라 기록하였다. 가열 후 생성물은 얼음물을 이용하여 식힌후 감압 여과한 뒤 항온항습기에서 60°C로 건조하였다. 이후 막자사발을 이용하여 곱게 갈아 20°C, 60RH% 조건에서 바이알에 보관하였다. 공정을 거친 리그닌 시료는 성분 분석, BET, SEM, NIR 분광분석을 통해 분석하였다. 획득된 NIR spectrum은 Unscrambler X 프로그램을 이용하여 SNV, Savitzky-Golay 2nd derivatives 수학 전처리 후 성분 분석, BET, SEM 분석 결과와 비교·분석되었다. 열수처리 유무 여부에대한 판정과 원소분석을 통해 얻은 탄소비율 추정을 부분최소자승법으로 분석한 결과 R-square 값이 각각 0.93과 0.91로 높은 정확도를 보였다. 1400-1500nm 파장대 등에서의 NIR 스펙트럼 피크값의 감소를 통해리그닌 작용기변화가 감지되었다. 본 연구에서 시도된 NIR 분광분석법에 의해 신속하고 비파괴적인 리그닌 열수처리 유무 여부 판정과 원소 함량비 검량이 기대된다.

Dkk3, downregulated in cervical cancer, functions as a negative regulator of β-catenin

Lee, Eun-Ju,Jo, Minwha,Rho, Seung Bae,Park, Kyoungsook,Yoo, Yae-Na,Park, Junsoo,Chae, Myounghee,Zhang, Wei,Lee, Je-Ho Wiley Subscription Services, Inc., A Wiley Company 2009 International journal of cancer: Journal internati Vol.124 No.2

<P>The Wnt/β-catenin signaling pathway is activated during the malignant transformation of keratinocytes that originate from the human uterine cervix. Dkk1, 2 and 4 have been shown to modulate the Wnt-induced stabilization of the β-catenin signaling pathway. However, the function of Dkk3 in this pathway is unknown. Comparison of the Dkk3 gene expression profiles in cervical cancer and normal cervical tissue by cDNA microarray and subsequent real-time PCR revealed that the Dkk3 gene is frequently downregulated in the cancer. Methylation studies showed that the promoter of Dkk3 was methylated in cervical cancer cell lines and 22 (31.4%) of 70 cervical cancer tissue specimens. This promoter methylation was associated with reduced expression of Dkk3 mRNA in the paired normal and tumor tissue samples. Further, the reintroduction of Dkk3 into HeLa cervical cancer cells resulted in reduced colony formation and retarded cell growth. The forced expression of Dkk3 markedly attenuated β-catenin-responsive luciferase activity in a dose-dependent manner and decreased the β-catenin levels. By utilizing a yeast two-hybrid screen, βTrCP, a negative regulator of β-catenin was identified as a novel Dkk3-interacting partner. Coexpression with βTrCP synergistically enhanced the inhibitory function of Dkk3 on β-catenin. The stable expression of Dkk3 blocks the nuclear translocation of β-catenin, resulting in downregulation of its downstream targets (VEGF and cylcin D), whereas knockdown of Dkk3 abrogates this blocking. We conclude from our finding that Dkk3 is a negative regulator of β-catenin and its downregulation contribute to an activation of the β-catenin signaling pathway. © 2008 Wiley-Liss, Inc.</P>

상품의 적합성평가제도에 있어 평가기준의 국가표준 일치화 정책방안 연구

이용하(Youngha Lee),배진한(JinHan Bae),이준수(Junsoo, Lee),송해근(Haegeun, Song) 인문사회과학기술융합학회 2018 예술인문사회융합멀티미디어논문지 Vol.8 No.3

점차 가속화 되는 글로벌 무역환경의 변화로 인해 제품의 자유로운 국경 간 이동이 활발하게 진행 되고 있으며, 소비자의 권익 향상으로 유통제품에 대한 상품의 품질, 안전, 그리고 서비스 등에 대한 소비자의 기대 수준 또한 높아지고 있다. 따라서 제조 유통업계는 상품에 대한 이러한 시장의 요구를 인증이라는 검증의 도구를 통해 입증하고 있으며, 정부는 이러한 인증 제도를 효율적으로 운영하기 위한 각종 인증 정책을 운영하고 있다. 본 연구는 인증이라는 용어로 통용되는 적합성평가제도에 있 어 평가기준으로 활용되는 국가표준 및 기술규정의 일치화 방안을 모색하고 인증제도간 효율적인 상 호인정을 촉진하는 제도적 개선방안을 도출하고자 한다. 이를 위해 본 연구는 첫째, 대한민국의 적합 성평가제도에 있어 표준 및 기술규정의 문제점에 대해 살펴보고 해외 사례 검토를 통한 활용현황을 분석한다. 둘째, 적합성평가에 대한 국가 간 또는 인증제도 간 상호인정에 대한 역학적 구조를 검토 한다. 셋째, 적합성평가제도 운영에 있어 이러한 표준의 활용 방향에 대해 살펴본다. As the global trade environment changes rapidly, the movement of products or services across the border is brisk. Along with this, the enhanced rights of consumer drives the requirements of quality, safety, and related services of distributed products is rising. The manufacturers and distributers prove that their products/services satisfy the market requirements by using verification tool such as certification system. In addition, the government operates certification policies to run the certification system efficiently. This study aims to deepen insight of the conformity assessment policy that is used as certification terminology in Korea and to find the agreement method of national standard and technical regulations which are used as evaluation criteria in the conformity assessment system. In addition, this study suggests the institutional improvement proposal that facilitates the mutual recognition between certification systems efficiently. For this, the current study firstly examines the problems of standard and technical regulations in Korea s conformity assessment system and reviews the foreign cases. Second, this article examines the dynamic structure of mutual recognition for the conformity assessment between countries. Finally, the utilization direction of the standard in the operation of conformity assessment suggested in this paper.

재배지역별 청양고추의 품질 특성

황인국(In Guk Hwang),김하윤(Ha Yun Kim),이준수(Junsoo Lee),김행란(Haeng-Ran Kim),조명철(Myeoung-Cheoul Cho),고인배(In Bae Ko),유선미(Seon Mi Yoo) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.9

본 연구에서는 우리나라의 청양고추를 13개 지역에서 노지재배된 것을 채취하여 재배지역별 품질 특성을 비교 분석하였다. 일반성분의 경우 단백질함량은 12.74~19.98%, 지방함량은 4.54~7.17%, 회분함량은 6.07~9.81% 범위로 넓은 분포를 보였다. 무기성분 함량은 지역별 차이를 보였지만 주요 무기성분은 K이었다. pH 및 총산도는 각각 4.86~5.26 및 2.10~4.25% 범위였다. 청양고추의 유리당은 fructose 및 glucose가 주요 유리당으로 검출되었고 각각 8.55~17.06% 및 3.25~10.47% 범위로 지역별 큰 차이를 나타냈다. 색도 중 a값은 28.89~37.12의 범위였고, ASTA값은 76.54~139.57의 범위로 a값에 비해 넓은 분포를 나타냈다. Capsaicin 함량은 100.27~261.54 mg%, dihydrocapsaicin 함량은 51.01~84.58 mg% 범위였고 capsaicinoid 함량은 11개 지역에서 200 mg% 이상의 함량을 보였다. 본 연구결과 청양고추는 재배지역에 따른 심한 함량 변이를 나타냈으며, 추후 다양한 품종을 대상으로 재배지역에 따른 품종 특성에 관한 추가 연구가 필요하다. This study was carried out to investigate the proximate composition, mineral content, pH, acidity, color, ASTA value, free sugar content, and capsaicinoid level of Cheongyang pepper (Capscium annuum L.) cultivated in 13 different regions. For proximate composition of Cheongyang pepper, protein, lipid, and ash contents showed wide ranges of 12.74~19.98%, 4.54~7.17%, and 6.07~9.81%, respectively. Calcium, magnesium, iron, sodium, and potassium are major minerals found in Cheongyang pepper. Further, pH, total acidity, a value, and ASTA value showed wide ranges of 4.86~5.26, 2.10~4.25%, 28.89~37.12, and 76.54~139.57, respectively. Free sugars were fructose (8.55~17.06%) and glucose (3.25~10.47%), but sucrose and maltose were not detected. Capsaicin and dihydrocapsaicin contents were in the range of 100.27 to 261.54 mg% and 51.01 to 84.58 mg%, respectively. Capsaicinoid contents were above 200 mg% for the 11 cultivation regions. According to our results, Cheongyang pepper may be affected by environmental conditions such as different cultivation region more than genotype.

미나리 에탄올 추출물의 항산화성분과 항산화활성

황초롱(Cho Rong Hwang),황인국(In Guk Hwang),김현영(Hyun Young Kim),강태수(Tae Soo Kang),김윤배(Yun Bae Kim),주성수(Sung Soo Joo),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.2

미나리 에탄올 추출물과 용매분획물에 대한 항산화물질과 항산화활성을 분석한 결과 미나리 에탄올 추출물의 총폴리페놀 함량은 37.50 ㎎ GA eq/g이었으며, 에틸아세테이트 층에서 240.61 ㎎ GA eq/g로 높게 나타났다. 총 플라보노이드 함량은 에탄올 추출물에서 26.50 ㎎ catechin eq/g 이었지만 에틸아세테이트 층에서는 105.57 ㎎ catechin eq/g의 함량을 나타내었다. DPPH법에 의한 항산화활성의 IC?? 값은 에탄올 추출물에서 1.07 ㎎/㎖이었으며, 에틸아세테이트 분획물에서는 0.08 ㎎/㎖로 높았다. 총 항산화력은 에탄올 추출물에서 34.48 ㎎ AA eq/g이었으며, 에틸아세테이트 층에서는 382.00 ㎎ AA eq/g으로 높았다. 환원력은 1 ㎎/㎖의 농도에서 에탄올 추출물은 0.45이었지만, 에틸아세테이트 층에서는 0.75로 높았다. Ferrous ion chelating 효과는 에탄올 추출물에서 32.01%로 높았고 용매 분획물에서는 오히려 감소하거나 없는 것으로 나타났으며 hydroxyl 라디칼 소거능의 IC??은 에틸아세테이트 분획물에서 26.71μg/㎖로 가장 높았다. This study was conducted to investigate the antioxidant compounds and antioxidant activity on the dropwort (Oenanthe javanica) and its solvent fraction. The dropwort was extracted with 70% (v/v) ethanol, and then partitioned using the solvents of hexane, chloroform, ethyl acetate, butanol, and aqueous. The ethyl acetate fraction contained the highest phenolic and flavonoid of 240.61 ㎎ GA eq/g and 105.57 ㎎ catechin eq/g, followed by ethanol extract of 37.50 ㎎ GA eq/g and 26.50 ㎎ catechin eq/g, respectively. The DPPH radical scavenging activity (IC??) on the solvent fractions increased in the order of ethyl acetate, butanol, ethanol extract, chloroform, aqueous, and hexane with 0.08, 0.58, 1.07, 2.43, 2.47, and 3.31 ㎎/㎖, respectively. The ABTS radical scavenging activity was the highest value of 382 ㎎ AA eq/g in ethyl acetate fraction. Reducing power and chelating effect on the ethanol extracts and its solvent fraction were in range of 0.23~0.75 and 0~32.01%, respectively. Hydroxyl radical scavenging activity (IC??) was the lowest value of 26.71 μg/㎖ in ethyl acetate fraction.