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기업용 SNS 를 활용한 S/W 유지보수 프로세스 연구
박정훈 ( Jung-hoon Park ),이정빈 ( Jung-been Lee ),이동현 ( Dong-hyun Lee ),인호 ( Hoh Peter In ) 한국정보처리학회 2011 한국정보처리학회 학술대회논문집 Vol.18 No.2
대규모 프로젝트는 여러 부서의 조직적이며 집중적으로 관리가 되고 있다. 그러나, 소규모 프로젝트나 자체 운영 유지보수에 대한 관리는 그에 미치지 못하고 있으며, 긴급한 이슈가 발생시 전사 대상 공지가 수월하지 않다는 문제점들이 있다. 이 문제 해결을 위해 접수부터 개발, 테스트, 이관의 각 단계에서 기업용 SNS 를 활용하여 부서간 업무 협업을 통한 품질보증 활동을 향상할 수 있는 응용방법을 제안하였다. 사례 연구를 통해 기업용 SNS 를 적용하여 소프트웨어 개발 유지보수 프로세스를 수행한 결과, 긴급 이슈 발생 건이 줄고, 조직의 구성원들의 이슈 확인에 대한 책임감 등 조직원의 품질에 대한 의식과 관심의 향상을 이끌어 내었다.
Structures of the γ‐class carbonic anhydrase homologue YrdA suggest a possible allosteric switch
Park, Hye‐,Mi,Park, Jeong‐,Hoh,Choi, Ji‐,Woo,Lee, Jieun,Kim, Bo Yeon,Jung, Che‐,Hun,Kim, Jeong‐,Sun International Union of Crystallography 2012 Acta crystallographica. Section D, Biological crys Vol.68 No.8
<P>The YrdA protein shows high sequence similarity to γ‐class carbonic anhydrase (γ‐CA) proteins and is classified as part of the γ‐CA protein family. However, its function has not been fully elucidated as it lacks several of the conserved residues that are considered to be necessary for γ‐CA catalysis. Interestingly, a homologue of γ‐CA from <I>Methanosarcina thermophila</I> and a β‐carboxysomal γ‐CA from a β‐cyanobacterium have shown that these catalytic residues are not always conserved in γ‐CAs. The crystal structure of YrdA from <I>Escherichia coli</I> (ecYrdA) is reported here in two crystallographic forms. The overall structure of ecYrdA is also similar to those of the γ‐CAs. One loop around the putative catalytic site shows a number of alternative conformations. A His residue (His70) on this loop coordinates with, or is reoriented from, the catalytic Zn<SUP>2+</SUP> ion; this is similar to the conformations mediated by an Asp residue on the catalytic loops of β‐CA proteins. One Trp residue (Trp171) also adopts two alternative conformations that may be related to the spatial positions of the catalytic loop. Even though significant CA activity could not be detected using purified ecYrdA, these structural features have potential functional implications for γ‐CA‐related proteins.</P>
Rapid Identification of Rickettsiae using the Real-Time PCR
Park, Hyo-Soon,Lee, Jung-Hee,Jin, Kwang-Hoon,Jang, Won-Jong,Park, Kyung-Hee,Kook, Yoon-Hoh,Lee, Seung-Hyun 대한미생물학회 2008 Journal of Bacteriology and Virology Vol.38 No.4
In this study, new real-time PCR method based on the groEL gene was developed and investigated. Four spotted fever group (SFG) strains, four typhus group (TG) strains, and four scrub typhus group (STG) strains were easily differentiated as a distinct entity. This PCR assay was applied to detect Rickettsia DNA from 100 ticks. Twelve Haemaphysalis longicornis ticks were found positive and identified as spotted fever group Rickettsia. This real-time PCR method could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of SFG, TG, and STG in a single reaction.