Intranasal delivery of the cytoplasmic domain of CTLA-4 using a novel protein transduction domain prevents allergic inflammation

Choi, Je-Min,Ahn, Mi-Hyun,Chae, Wook-Jin,Jung, Yung-Gook,Park, Jae-Chul,Song, Hyun-Mi,Kim, Young-Eun,Shin, Jung-Ah,Park, Choon-Sik,Park, Jung-Won,Park, Tae-Kwann,Lee, Jung-Hoon,Seo, Byung-Fhy,Kim, Kyu Nature Publishing Group 2006 Nature medicine Vol.12 No.5

CTLA-4 is a negative regulator of T-cell activation, and its inhibitory effects can be accomplished either by competition with CD28 or by transmitting negative signals through its intracellular domain. To utilize the cytoplasmic domain of CTLA-4 to suppress allergic inflammation, we fused it to a novel protein-transduction domain in the human transcriptional factor Hph-1. Transduction efficiency was verified in vitro and in vivo after ocular, intranasal and intradermal administration. After transduction into T cells, the Hph-1–ctCTLA-4 fusion protein inhibited the production of interleukin (IL)-2, and downregulated CD69 and CD25. Intranasal administration of Hph-1–ctCTLA-4 resulted in markedly reduced infiltration of inflammatory cells, secretion of T helper type 2 (T<SUB>H</SUB>2) cytokines, serum IgE levels and airway hyper-responsiveness in a mouse model of allergic airway inflammation. These results indicated that Hph-1–ctCTLA-4 constitutes an effective immunosuppressive protein drug for potential use in the treatment of allergic asthma, via nasal administration.

경골 Pilon. 골절의 수술적 치료 : 내고정과 외고정의 적응증 Appication of int. Fixation and Ext. Fixation

서정국,정승훈 대한골절학회 1997 대한골절학회지 Vol.10 No.1

We have reviewed 15 cases of tibial pilon fractures who have been treated operatively from May, 1990 to May, 1995 at our hospital. 1. The fractures were classified into Type I, II and III according to the system of Mast, Spiegel and Pappas and Type III were subdivided into Type A, B and C according to the system of Ruedi- Allgower. Type I was 1 case(6.7%). Type II were 8(53.3%), Type IIIA were 2(l3.3%), Type IIIB were 3(20%) and Type IIIC was 1 case(6.7%). 2. Type I, II and IIIA were treated with open reduction and internal fixation using screw or plate. 2 cases of Type IIIB were treated with open reduction and external fixation and 1 case was treated with piate. Type IIIC was treated with open reduction and external fixation. 3. Conclusively, we had good results with open reduction and internal fixation in Type I, lI and IIIA. In Type lll8, IIIC, if it was difficult to maintain of reduction with internal fixation due to severe bone loss, comminution and soft tissue injury, we could have goad results with open reduction, bone graft and external fixation including ankle joint.

동통을 동반한 비골의 불유합

정병현,신규호,안종국,김병직,서광윤 인제대학교 1987 仁濟醫學 Vol.8 No.3

동통성 비골 골절의 불유합 2례를 금속판 내고정으로 치료하여 각 3개월과 5개월에 동통 소실과 골유합을 얻었기에 보고하는 바이다. Two cases of symptomatic fibula nonunion were presented with a brief review of the literatures. The patients were treated with open reduction and plate and screws and goad results were achieved.

경추 척수증에서 불충분한 전방 감압술후 추궁판 성형술 5례 보고

서정국,김정훈 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Objective: We have analyzed the clinical and radiological outcomes in 5 patients who showed no improvement after anterior decompression so that underwent laminoplasty. Materials and Methods: We studied retrospectively 5 patients who underwent laminoplasty after anterior decompression between January 1998 and April 1999. The mean age was 55.4 years (46 to 60). The mean duration from anterior decompression to laminoplasty was 39 months (12 to 90). We evaluated clinical symptoms and signs. Plain radiogram and MRI were taken before and after surgery. In evaluating the clinical results, the evaluation system established by the Japanese Orthopedic Association (JOA score) and Hirabayashi recovery rate was employed. Results: Postoperative radiograms showed an incresasement of body to canal ratios(average 0.79 pre-op, to 1.15 post-op.) and the mean JOA score increased from 11.8 preoperatively to 14.4 at the final follow-up with a mean recovery rate of 48%. Conclusion: It is better to do anterior decompression in patients who have one or two segments involved. Otherwise, patients who involve more than 3 segments can be managed using laminoplasty. Laminoplasty for patients who underwent incomplete ant, decompression may be useful method.

포도 ‘거봉’ 품종에 있어서 착립 정도와 과실 품질과의 관계

박서준(Seo Jun Park),김진국(Jin Gook Kim),정성민(Sung Min Jung),노정호(Jung Ho Noh),허윤영(Youn Young Hur),류명상(Myung Sang Ryou),이한찬(Han Chan Lee) 한국원예학회 2010 원예과학기술지 Vol.28 No.6

포도 ‘거봉’ 품종의 적정 착과량 설정을 위한 기초자료를 마련하고자 신초당 착립수를 조절하여 변색기 이후의 과실의 품질변화를 조사하였다. 과실의 당도는 변색기 이후 30일까지 급격히 증가하였으며, 신초당 착립수가 적을수록 빠르게 증가하였다. 신초당 10립과 20립 처리구의 당도는 변색기 이후 4주까지 18.0°Bx 이상 급격히 상승한 다음, 완만하게 증가하였다. 신초당 30립 처리구의 당도는 변색초기에는 느리게 상승하였으나, 수확기까지 지속적으로 상승하여 18.0°Bx에 도달하였다. 그러나 신초당 40립과 50립 처리구는 변색기 이후 4주까지도 당도의 상승폭이 현저히 적었고, 성숙기에 도달해도 15.0°Bx에 그쳤다. 과즙의 산 함량은 모든 처리구에서 변색기 이후 30일까지 빠르게 감소하였고, 그 이후에는 완만하게 감소하였으며, 신초당 50립 처리구를 제외한 모든 처리구의 산 함량은 0.4-0.6%에 도달하였다. 과피색은 변색기 이후 모든 처리구에서 성숙이 진행됨에 따라 녹색에서 자흑색으로 변화하면서 L, b값은 감소하였고, a값은 증가하였다. 총 안토시아닌 함량은 신초당 10립과 20립 처리구의 경우 변색기 이후 2주까지 완만히 증가한 후 변색기 이후 6주까지 급격히 증가하였고, 그 후에는 감소하는 양상을 나타내었으며, 신초당 30, 40 및 50개의 착립구는 정상적인 착색을 나타내지 못하였다. 따라서 본 연구결과 과실품질을 고려할 때 거봉 포도의 착립 정도는 신초당 20립 내외가 적당한 것으로 나타났다. The study was conducted to investigate the relationship between berry set density per bearing shoot and fruit quality in ‘Kyoho’ grape. Fruit quality was evaluated by soluble solids content (SSC), titratable acidity (TA), skin color, and total anthocyanin content (TAC). There was a sharp increase in SSC of fruit juice from veraison initiation to 30 days after veraison. The SSC level increased with the lower berry set per bearing shoot. The rapid accumulation of SSC 4 weeks after veraison in the cluster of low berry set (10-20) was observed, while that of high berry set (40-50) showed delayed and low SSC accumulation which reached only 15.0°Bx at harvest. TA of fruit juice in all treatments reduced rapidly from veraison initiation to 30 days after veraison, and then reduced gradually. TA in all treatments except 50 berries set reached to 0.4-0.6% which was optimal TA in ‘Kyoho’ at fruit harvest. During fruit maturing after veraison, fruit skin color changed from green to purple-black, L and b value decreased, and a value increased. TAC in 10 and 20 berries set was gradually increased to 2 weeks after veraison, sharply increased until 6 weeks after veraison, and then finally decreased, while TAC in content in 30, 40, and 50 berries set per annual shoot remained at low level. The results indicated that the number of berry set for good quality seemed to be 20 per bearing shoot in ‘Kyoho’ grape.

Low Cost MEMS-IMU Based DR/GPS Integrated System in Urban Environment

Woo Jung Park,Jae Hong Lee,Chang Ho Kang,Myung Hwan Seo,Sang Yeon Park,Jong Yun Yeo,Jin Woo Song,Chan Gook Park 제어로봇시스템학회 2018 제어로봇시스템학회 국제학술대회 논문집 Vol.2018 No.10

In this paper, we propose an MEMS-IMU Based 10<SUP>th</SUP> order DR/GPS integrated system. Existing low cost MEMS based INS/GPS integrated systems are not applicable to autonomous vehicle localization that needs navigation error under 3m. To improve the performance of the MEMS based navigation system, odometer based DR/GPS formulation was proposed. By substituting MEMS accelerometer to odometer, velocity and position errors of navigation solution are reduced. The proposed method is tested for a land vehicle in urban area including GPS outage section. The experimental results show that the proposed method reduced the navigation error in the GPS outage section than MEMS INS/GPS system.

흉요추부 골다공증성 압박골절에서 골절부 압박 진행 정도 및 골 밀도 검사 수치와의 상관관계

김정훈 ( Jung Hoon Kim ),서정국 ( Jeong Gook Seo ),안종호 ( Jong Ho Ahn ) 대한골절학회 2006 대한골절학회지 Vol.19 No.2

목적: 보존적 요법으로 치료한 흉요추부 골다공증성 압박골절 환자에서 골절부 압박 진행 정도와 골 밀도 검사 수치와의 상관관계를 알아보고자 하였다. 대상 및 방법: 2002년 3월부터 2005년 3월까지 가벼운 외상에 의해 흉요추부 골다공증성 압박굴절로 진단 받고 보존적 요법으로 치료한 30예를 대상으로 입원 당시 시행한 골 밀도 검사 수치에 따라 80% 이상, 70~80%, 60~70%, 60% 이하의 4군으로 분류하였으며 수상 당시, 1개월, 2개월, 3개월 후의 단순 방사선 사진상의 시상지수 및 전방 주체 높이를 측정하여 비교하였다. 결과: 골 밀도 수치가 낮은 군에서 수상 당시 압박의 정도가 더 심하였고 골 밀도 검사치가 낮은 군일수록 압박의 진행 정도가 크게 나타났으며 골 밀도 수치와 상관없이 각 군에서 수상 후 1개월에 압박의 진행 정도가 가장 호전되었다. 결론: 흉요추부 골다공증성 압박골절 환자에서 골 밀도 검사치가 낮은 경우에 압박의 진행을 예상하고 보다 적극적인 조기 보조기 착용 및 장기간의 골다공증 치료가 필요할 것이라 생각한다. Purpose: To evaluate whether progression of compression correlates with bone densiometry index in patients who were treated conservatively for osteoporotic compression fracture of thoracolumbar spine. Materials and Methods: Using the results of bone densiometry, 30 patients who were treated conservatively for osteoporotic compression fracture of thoracolumbar spine between March 2002 to March 2005 were categorized into 4 groups ; above 80%, 70 to 80%, 60 to 70%, and below 60%. We compared the measurements of sagittal index and anterior vertebral height from the plain radiographs taken at the time of injury and following three consecutive months after the injury. Results: Patients with lower bone densiometry index had greater amount of compression at the time of injury and more rapid progression of compression. We also found that progression of compression was lowest during the first month after injury in all groups. Conclusion: Patients with low bone densiometry index in osteoporotic thoracolumbar compression fracture are susceptible to more rapid progression of compression and should have early brace application and longer duration of treatment for osteoporosis.

포도 ‘캠벨얼리’ 품종의 엽수 조절에 따른 과실 품질변화

박서준(Seo Jun Park),김진국(Jin Gook Kim),정성민(Sung Min Jung),노정호(Jung Ho Noh),허윤영(Youn Young Hur),박교선(Kyo Sun Park) (사)한국생물환경조절학회 2011 시설원예‧식물공장 Vol.20 No.3

포도 ‘캠벨얼리’ 품종의 결과지당 적정 엽수를 구명하기 위해 만개 30일 후에 결과지당 2송이를 착과시키고, 첫 번째 송이는 70~75립, 두 번째 송이는 35~40립으로 조절하고 변색기 이후 과실의 품질변화를 조사하였다. 과실의 당도는 변색기 이후 28일 동안 급격히 증가하였으나, 그 이후에는 일정한 수준을 유지하였다. 결과지당 15엽 처리구는 착색초기부터 빠르게 증가되어 착색 28일 후에 16.0°Bx 이상 되었으나, 결과지당 10엽 처리구는 12.0°Bx로 증가폭이 적었고, 결과지당 5엽 및 0엽 처리구는 당도 축적이 거의 없었다. 산도는 모든 처리구에서 28일 동안 감소되었고, 결과지당 엽수가 많을수록 감소폭도 컸다. 결과지당 15엽 처리구는 변색기 이후 7일 동안 빠르게 감소된 후 수확기까지 꾸준히 감소되어 0.5%로 되었으나, 결과지당 5엽 및 0엽 처리구는 감소세가 완만하여 수확기에도 산 함량이 1.0%로 높았다. 과피색은 결과지당 15엽에서 hunter L과 b값이 변색기 이후 7일 동안 빠르게 감소되었고, 그 이후에는 완만하게 감소되어 수확기에 가장 낮은 값을 나타냈고, hunter a값은 변색기 이후 7일 동안 빠르게 증가되었고, 그 이후에는 약간 감소되었다. 그러나 결과지당 5엽 및 0엽 처리구는 hunter L과 b값이 변색기부터 완만하게 감소되었고, hunter a 값은 착색초기에는 현저히 낮았으나, 수확기까지 꾸준히 증가되었다. 총 안토시아닌은 결과지당 15엽 처리구에서 변색기 7일 후부터 빠르게 증가된 후 변색기 28일 후부터는 일정한 수준을 유지하였고, 다른 처리구는 변색기 이후 큰 차이가 없었다. This study aimed to determine optimum leaves per shoot in ‘Campbell Early’ grape through investigating changes of fruit quality after veraison 70~75 berries of first cluster and 35~40 berries of second cluster at 30 days after full bloom. Soluble solids content (SSC) increased dramatically during 28 days after veraison and afterwards it was stabilized. 15 leaves per shoot treatment showed fast increase of SSC from early veraison and reached to over 16.0°Bx at 28 days after veraison. No considerable increase of SSC were occurred on either 5 or 0 leaves per shoot treatments after veraison. Titratable acidity (TA) was decreased continuously during 28 days of berry ripening in the all treatments. In 15 leaves per shoot treatment, TA decreased fast during first 7 days after veraison and continuously decreased to 0.5% by harvest time. While, 5 and 0 leaves per shoot treatments showed TA decrease slightly only at the early veraison. Hunter L and b values of 15 leaves per shoot treatment decreased fast during first 7 days of veraison, and gradually decreased by 14 days, whereas Hunter a value increased fast during the first 7 days from veraison. Total anthocyanin content in 15 leaves per shoot treatment increased fast from 7 days of veraison and the increase became slow after 28 days of veraison. Other treatments showed no significant difference from veraison to harvest time.

체감형 VR 게임이 기분상태 변화와 운동 효과에 미치는 영향

서성우 ( Seo Seong-woo ),고경훈 ( Ko Kyung-hoon ),박상국 ( Park Sang-gook ),허정훈 ( Huh Jung-hoon ) 한국스포츠심리학회 2021 한국스포츠심리학회지 Vol.32 No.4

목적: 본 연구는 체감형 VR 게임이 기분상태와 운동 효과에 어떠한 영향을 미치는지 검증하는 데 목적이 있다. 방법: 연구참여자는 무선할당에 의해 VR 신체활동 게임집단 9명, 오프라인 신체활동 집단 9명으로 분류하였다. 4주 동안 실시한 프로그램에서 수집된 데이터를 바탕으로 반복측정 분산분석(RM ANOVA) 방법을 사용하여 기분 상태 변화 수준과 운동 효과의 차이를 확인했다. 결과: 두 집단 모두 기분상태의 부정적 요인이 감소하고 긍정적 요인은 증가한 것으로 나타났다. 반면 집단 간에 유의한 차이는 발견되지 않았다. 최대심박수와 운동 자각 수준이 유의하게 감소하여 운동 효과가 있는 것으로 나타났다. 결론: 체감형 VR 게임이 기분상태의 부정적 요인을 낮추고 긍정적 요인을 증가시킨다는 것을 알 수 있었고 중요하게는 오프라인에서 실시하는 신체활동과 유사한 수준의 기분상태 변화와 운동 효과가 있다는 것을 검증하였다. 시·공간의 제약을 받지 않고 혼자서도 즐길 수 있는 체감형 VR 게임은 코로나19 이후 신체활동의 대체물로써 최적의 사용자경험을 제공할 것으로 기대되며 장애·비만·노인 등 신체활동에 제한이 있는 사람들을 위한 맞춤형 프로그램 개발이 활발하게 이루어져야 할 것으로 논의하였다. Purpose: The purpose of this study is to analyze differences in mood conditions and exercise effects among groups and provide basic data on physical activities after Covid-19 by conducting a motion-based VR game program for four weeks. Methods: Physical activity program participants were classified into two groups(Experimental group, 9; Comparison group, 9) by random assignments. Based on the data collected for four weeks, the repeated measures ANOVA(RM ANOVA) method was used to determine the difference in mood state change levels and motor effects between groups. Results: It was found that both groups reduced negative factors in mood and improved positive factors. On the other hand, no significant difference was found between groups. The maximum heart rate and exercise awareness level were significantly reduced, indicating that there was an exercise effect. Conclusion: The above results are significant in that they verified that motion-based VR games lower negative factors of mood and improve positive factors, and that they have a similar level of exercise effect to offline physical activities. motion-based VR games that can be enjoyed alone without time and space constraints are expected to provide optimal user experience as a substitute for physical activities after COVID-19, and customized programs for people with limited physical activities such as disabilities, obesity, and the elderly should be actively developed.

퇴행성 슬관절염 환자에서 슬관절경 세척술과 경피적 다발성 골천공술의 치료 결과분석

박정규,김진구,김병직,임영,고한석,서정국,안치성 대한슬관절학회 1997 대한슬관절학회지 Vol.9 No.1

42 knees with degenerative arthritis underwent arthroscopic lavage and 7 knees underwent arthroscopic lavage combined with multiple bone drilling in between 1994 and 1995 and followed average 12 months. Clinical evaluation using the hospital for special surgery knee scores(HSS scores) was done at last follow up and classified into 4 groups: excellent(90-99), good(80-89), fair(70-79) and poor(less than 70). The results were as follows: 1. Of the 42 knees of the lavage group, 6 knees(16%) was excellent, 14 knees (33%) good, 17 knees(40%) fair and 5(11%) knees poor. Success group (Excellent & Good) were achived in 20 knees(49%). 2. Of 17 knees treated with arthroscopic lavage comhined with multiple bone drillig resulted in excellent in 9 knees(53%), good in 4 knees(23%), fair in 2 knees(12%) and poor in 2 knees(12%). Success group were achived in 13 knees(76%). In conclusion, we believe that multiple bone drilling in conjunction with arthroscopic lavage has more favorable effect on the pain relief for degenerative osteoarthritis of the knee than arthroscopic lavage only.