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Marzhan Seitovna Kalmakhanova,Jose Luis Diaz de Tuesta,Bakytgul Kabykenovna Massalimo,Helder Teixeira Gomes 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.2
Pillared clays with Zr and Fe/Cu/Zr polycations have been prepared from natural clays found in large deposits of Kazakhstan and assessed as catalysts for the catalytic wet peroxide oxidation (CWPO), using 4-nitrophenol (4-NP) as model compound. The performance of the catalysts was followed by measuring the concentration of 4-NP, H₂O₂ and the total organic carbon (TOC), considering C4-NP = 5 g L<SUP>-1</SUP>, CH₂O₂ = 17.8 g L<SUP>-1</SUP>, Ccat = 2.5 g L<SUP>-1</SUP>, initial pH = 3.0 and T = 50°C. At those selected conditions, the pillared clays showed higher activity than natural clays in the CWPO of 4-NP. The conversion of the model pollutant was complete when Fe/Cu/Zr-PILCs were used, with the TOC removal reaching 78.4% after 24 h with the best Fe/Cu/Zr-PILC. The H₂O₂, 4-NP and TOC time-evolution was well described by a kinetic model based on TOC lumps in three blocks, considering the initial TOC (corresponding to 4-NP), the production of oxidizable intermediates and the formation of refractory products.
Definition of a Methodology for an Expedited Assessment of Slope Instability on Volcanic Terrains
( Ana Maria Malheiro ),( Luis Teixeira ),( Paulo Amaral ),( Filipe Marques ),( Jose Vieira ),( Leticia Moniz ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2
A project called MACASTAB (co-financed by the Interreg Mac 2014-20 program) is being developed, by a group of technicians from the Canarias, Azores, Madeira and Cabo Verde archipelagos. This project aims to prepare a methodological guide for the natural risk management produced by the instability of volcanic slopes and talus in Macaronesia archipelagos. One of its tasks is the development of charts that can be applied in the referred archipelagos in order to do an expeditious evaluation of slope stability. Given the specificities of the geology of the Azores and Madeira islands, the charts were supported by limit equilibrium analysis methods (e.g. Morgenstern-Price) applied to slopes whose constitution is predominantly formed by soils (an unusual situation in other archipelagos that are in the project). Results of shear strength parameters obtained for volcanic soils on both archipelagos by triaxial compression tests and direct shear tests are presented. Empirical correlation between friction angle and particle size distribution was found with a coefficient of adjustment of 93%. That correlation shows a relationship between the angle of internal friction of a given material and the percentage of gravel and sand. For the preparation of these charts, several stability analyzes were performed with different scenarios (slope gradient and heights, and shear strength parameters), in order to determine the safety factor of slopes in a simple and fast way in the field.
Applications of Yeast Flocculation in Biotechnological Processes
Domingues, Lucilia,Vicente, Antonio A.,Lima, Nelson,Teixeira, Jose A. The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.4
A review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects-the basics of yeast flocculation, the development of "new" flocculating yeast strains and bioreactor development. In what concerns the basics of yeast flocculation, the state of the art on the most relevant aspects of mechanism, physiology and genetics of yeast flocculation is reported. The construction of flocculating yeast strains includes not only the recombinant constitutive flocculent brewer's yeast, but also recombinant flocculent yeast for lactose metabolisation and ethanol production. Furthermore, recent work on the heterologous $\beta$-galactosidase production using a recombinant flocculent Saccharomyces cerevisiae is considered. As bioreactors using flocculating yeast cells have particular properties, mainly associated with a high solid phase hold-up, a section dedicated to its operation is presented. Aspects such as bioreactor productivity and culture stability as well as bioreactor hydrodynamics and mass transfer properties of flocculating cell cultures are considered. Finally, the paper concludes describing some of the applications of high cell density flocculating bioreactors and discussing potential new uses of these systems.e systems.
( Jorge Frias ),( Duarte Toubarro ),( Alexandra Fraga ),( Claudia Botelho ),( Jose Teixeira ),( Jorge Pedrosa ),( Nelson Simoes ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.2
Fibrinolytic enzymes with a direct mechanism of action and safer properties are currently requested for thrombolytic therapy. This paper reports on a new enzyme capable of degrading blood clots directly without impairing blood coagulation. This enzyme is also non-cytotoxic and constitutes an alternative to other thrombolytic enzymes known to cause undesired side effects. Twenty-four Bacillus isolates were screened for production of fibrinolytic enzymes using a fibrin agar plate. Based on produced activity, isolate S127e was selected and identified as B. subtilis using the 16S rDNA gene sequence. This strain is of biotechnological interest for producing high fibrinolytic yield and consequently has potential in the industrial field. The purified fibrinolytic enzyme has a molecular mass of 27.3 kDa, a predicted pI of 6.6, and a maximal affinity for Ala-Ala-Pro-Phe. This enzyme was almost completely inhibited by chymostatin with optimal activity at 48℃ and pH 7. Specific subtilisin features were found in the gene sequence, indicating that this enzyme belongs to the BPN group of the S8 subtilisin family and was assigned as AprE127. This subtilisin increased thromboplastin time by 3.7% (37.6 to 39 s) and prothrombin time by 3.2% (12.6 to 13 s), both within normal ranges. In a whole blood euglobulin assay, this enzyme did not impair coagulation but reduced lysis time significantly. Moreover, in an in vitro assay, AprE127 completely dissolved a thrombus of about 1 cc within 50 min and, in vivo, reduced a thrombus prompted in a rat tail by 11.4% in 24 h compared to non-treated animals.
Lubrication Aspects during Single Point Incremental Forming for Steel and Aluminum Materials
Nelson Gil Azevedo,João Sá Farias,Ricardo Pereira Bastos,Pedro Teixeira,João Paulo Davim,Ricardo Jose Alves de Sousa 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.
Over the past few years, Single Point Incremental Forming (SPIF) has been mainly studied regarding formability, forming forcesinvolved and the dimensional accuracy achieved in the final product. As in any other metal forming process, the choice of thelubricant depends on the deformation mechanisms that characterize the process, the roughness of the material involved and on theprocess parameters. Despite the recent developments on SPIF, little attention has been paid to the influence of lubricants used duringthe process. This study intends to evaluate the influence of the type of lubricant used in SPIF process, particularly on aluminum 1050and DP780 steel sheets, in what concerns the surface quality of final parts. To do so, tests were performed employing a range ofdistinct lubricants. Roughness tests were conducted to evaluate surface quality. Results show opposite trends for aluminum and steelin the sense that lubricants that guarantee better results in aluminum proved to have worse results in steel and vice-versa.