Corni Fructus attenuates testosterone-induced benign prostatic hyperplasia by suppressing 5α-reductase and androgen receptor expression in rats

Hwangbo, Hyun,Kwon, Da He,Choi, Eun Ok,Kim, Min Yeong,Ahn, Kyu Im,Ji, Seon Yeong,Kim, Jong Sik,Kim, Kyung-Il,Park, No-Jin,Kim, Bum Hoi,Kim, Gi-Young,Hong, Su-Hyun,Park, Cheol,Jeong, Ji-Suk,Choi, Yung The Korean Nutrition Society 2018 Nutrition Research and Practice Vol.12 No.5

BACKGROUND/OBJECTIVES: Benign prostatic hypertrophy (BPH) is a major cause of abnormal overgrowth of the prostate mainly in the elderly. Corni Fructus has been reported to be effective in the prevention and treatment of various diseases because of its strong antioxidant effect, but its efficacy against BPH is not yet known. This study was designed to evaluate the therapeutic efficacy of Corni Fructus water extract (CF) in testosterone-induced BPH rats. MATERIALS/METHODS: To induce BPH, rats were intraperitoneal injected with testosterone propionate (TP). Rats in the treatment group were orally administered with CF with TP injection, and finasteride, which is a selective inhibitor of $5{\alpha}$-reductase type 2, was used as a positive control. RESULTS: Our results showed that the increased prostate weight and histopathological changes in BPH model rats were suppressed by CF treatment. CF, similar to the finasteride-treated group, decreased the levels of testosterone and dihydrotestosterone by TP treatment in the serum, and it also reduced $5{\alpha}$-reductase expression and concentration in prostate tissue and serum, respectively. In addition, CF significantly blocked the expression of the androgen receptor (AR), AR co-activators, and proliferating cell nuclear antigen in BPH rats, and this blocking was associated with a decrease in prostate-specific antigen levels in serum and prostate tissue. CONCLUSIONS: These results suggest that CF may weaken the BPH status through the inactivation of at least $5{\alpha}$-reductase and AR activity and may be useful for the clinical treatment of BPH.

Corni Fructus attenuates testosterone-induced benign prostatic hyperplasia by suppressing 5α-reductase and androgen receptor expression in rats

Hyun Hwangbo,Da He Kwon,Eun Ok Choi,Min Yeong Kim,Kyu Im Ahn,Seon Yeong Ji,Jong Sik Kim,Kyung-Il Kim,No-Jin Park,Bum Hoi Kim,Gi-Young Kim,Su-Hyun Hong,Cheol Park,Ji-Suk Jeong,Yung Hyun Choi 대한지역사회영양학회 2018 Nutrition Research and Practice Vol.12 No.5

BACKGROUND/OBJECTIVES: Benign prostatic hypertrophy (BPH) is a major cause of abnormal overgrowth of the prostate mainly in the elderly. Corni Fructus has been reported to be effective in the prevention and treatment of various diseases because of its strong antioxidant effect, but its efficacy against BPH is not yet known. This study was designed to evaluate the therapeutic efficacy of Corni Fructus water extract (CF) in testosterone-induced BPH rats. MATERIALS/METHODS: To induce BPH, rats were intraperitoneal injected with testosterone propionate (TP). Rats in the treatment group were orally administered with CF with TP injection, and finasteride, which is a selective inhibitor of 5α-reductase type 2, was used as a positive control. RESULTS: Our results showed that the increased prostate weight and histopathological changes in BPH model rats were suppressed by CF treatment. CF, similar to the finasteride-treated group, decreased the levels of testosterone and dihydrotestosterone by TP treatment in the serum, and it also reduced 5α-reductase expression and concentration in prostate tissue and serum, respectively. In addition, CF significantly blocked the expression of the androgen receptor (AR), AR co-activators, and proliferating cell nuclear antigen in BPH rats, and this blocking was associated with a decrease in prostate-specific antigen levels in serum and prostate tissue. CONCLUSIONS: These results suggest that CF may weaken the BPH status through the inactivation of at least 5α-reductase and AR activity and may be useful for the clinical treatment of BPH.

Application of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Following Integrated PET/CT in Mediastinal Staging of Potentially Operable Non-small Cell Lung Cancer

Hwangbo, Bin,Kim, Seok Ki,Lee, Hee-Seok,Lee, Hyun Sung,Kim, Moon Soo,Lee, Jong Mog,Kim, Hyae-Young,Lee, Geon-Kook,Nam, Byung-Ho,Zo, Jae Ill Elsevier 2009 Chest Vol.135 No.5

<P>BACKGROUND: The role of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) following integrated PET/CT scanning in mediastinal staging of non-small cell lung cancer (NSCLC) has not been assessed. METHODS: We prospectively evaluated the diagnostic values of PET/CT scanning and EBUS-TBNA for mediastinal staging in 117 patients with potentially operable NSCLC with accessible mediastinal lymph nodes (diameter range, 5 to 20 mm) by EBUS-TBNA. Subgroup analysis according to histologic type was performed. RESULTS: Of 30 cases of mediastinal metastasis, 27 were confirmed by EBUS-TBNA and 3 were confirmed by surgery. EBUS-TBNA results confirmed all cases with true-positive PET/CT scan findings and six of nine cases with false-negative PET/CT scan findings. The sensitivity, specificity, positive predictive value, negative predictive value (NPV), and accuracy of EBUS-TBNA in the detection of mediastinal metastasis were 90.0%, 100%, 100%, 96.7%, and 97.4%, respectively. For PET/CT scans, the values were 70.0%, 59.8%, 37.5%, 85.2%, and 62.4%, respectively (p = 0.052; p < 0.001; p < 0.001; p = 0.011; p < 0.001, respectively). In adenocarcinoma (n = 55), EBUS-TBNA detected four of six cases with false-negative PET/CT scan findings, and the NPV was higher for EBUS-TBNA than for PET/CT scans (94.6% vs 77.8%, respectively; p = 0.044). In squamous cell carcinoma (n = 53), the NPV of EBUS-TBNA and PET/CT scans were similarly high (97.9% vs 96.3%, respectively; p = 0.689). CONCLUSIONS: EBUS-TBNA was an effective invasive method following PET/CT scanning in the mediastinal staging of potentially operable NSCLC. In mediastinal PET/CT scan-positive cases, EBUS-TBNA was an excellent tool for detecting mediastinal metastasis. Even in mediastinal PET/CT scan-negative cases, EBUS-TBNA can be useful for confirming mediastinal metastases, especially in adenocarcinoma.</P>

Poster Session : In vitro assay of human telomerase reverse transcriptase and human telomerase rna components expressed in escherichia coli

( Jong Uk Koh ),( Hyun Suk Hwangbo ),( Kwang Hoon Kong ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-

Interaction of Norfloxacin with Super-Coiled DNA

Hwangbo, Hyun-Jung,Lee, Young-Ae,Park, Jung-Hag,Lee, Yong-Rok,Kim, Jong-Moon,Yi, Seh-Yoon,Kim, Seog K. Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.5

Norfloxacin, that inhibits the action of topoisomerase Ⅱ, binds to wide variety of DNA. The binding mode of this drug to double- and super-coiled DNA (ds- and scDNA) is compared in this study by various spectroscopic methods, including absorption, fluorescence, and circular dichroism(CD) spectroscopy. Hypochromism in the absorption band, negative and positive induced CD bands (respectively in 240-260 nm and 270-300 nm region) are apparent for the norfloxacin that bound to both the dsDNA and scDNA. A decrease in fluorescence is also noticed in the presence of both DNAs. Since the spectroscopic characteristics are the same for both complexes, it is imperative that the binding mode of the norfloxacin is similar in ds- and scDNA. In the presence of $Mg^{2+}$, which is a cofactor in the topoisomerase Ⅱ action, the fluorescence intensity of the scDNA-norfloxacin complex increased and the resulting fluorescence intensity and shape was identical to that in the absence of scDNA. Therefore, the addition of an excess amount of $Mg^{2+}$ may result in the extrusion of norfloxacin from scDNA.

Transoesophageal needle aspiration using a convex probe ultrasonic bronchoscope

HWANGBO, Bin,LEE, Hee Seok,LEE, Geon-Kook,LIM, Kun-Young,LEE, Soo-Hyun,KIM, Hyae-Young,LEE, Jong-Yeul,ZO, Jae Ill Blackwell Publishing Asia 2009 Respirology Vol.14 No.6

<P>ABSTRACT</P><P>Background and objective: </P><P>Although endoscopic ultrasound-guided fine needle aspiration can be helpful when combined with bronchoscopic procedures, endoscopic ultrasound-guided fine needle aspiration is not available as a conjunctive procedure with bronchoscopy at many institutions. This study evaluated the feasibility and the additional role of transoesophageal fine needle aspiration using a convex probe ultrasonic bronchoscope (EUS-B-FNA).</P><P>Methods: </P><P>We analysed 84 patients who underwent EUS-B-FNA between Oct 2007 and May 2008. Bronchoscopy and/or endobronchial ultrasound-guided transbronchial needle aspiration was performed on 83 patients prior to EUS-B-FNA.</P><P>Results: </P><P>EUS-B-FNA was performed on 89 lesions (1.7 aspirations/lesion) including three lung masses and 86 lymph nodes (nodal stations 1, 3P, 4L, 5, 7, 8, 9 and 10L) without complication. Sample adequacy was 95.4% for each aspiration and 100% for each lesion. Of the 89 lesions, 39 malignant lesions were confirmed by EUS-B-FNA. EUS-B-FNA provided additional diagnostic gain to bronchoscopic procedures in 16 patients (19.0%): 3 lung cancers were upstaged, 11 lung cancers were pathologically confirmed, and 2 patients were diagnosed with mediastinal metastasis from an extrathoracic malignancy. This gain was obtained by the sampling of inaccessible (<I>n</I> = 4) or difficult lesions by endobronchial ultrasound-guided transbronchial needle aspiration (<I>n</I> = 2) or when bronchoscopy was difficult due to dyspnoea, cough, brain metastasis or other conditions (<I>n</I> = 10).</P><P>Conclusions: </P><P>EUS-B-FNA is a technically feasible and safe procedure, which may be an alternative to endoscopic ultrasound-guided fine needle aspiration as a procedure that complements bronchoscopy. Additional diagnostic yield can be obtained by combining EUS-B-FNA with bronchoscopic procedures.</P>

Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100

HwangBo, Kwon,Son, Su-Hyun,Lee, Jong-Suk,Min, Sung-Ran,Ko, Suk-Min,Liu, Jang-R.,Choi, Dong-Su,Jeong, Won-Joong The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.1

A DNA extraction method using Chelex 100 is widely used for bacteria, Chlamydomonas, and animal cell lines, but only rarely for plant materials due to the need for additional time-consuming and tedious steps. We have modified the Chelex 100 protocol and successfully developed a rapid and simple method of DNA extraction for efficient PCR-based detection of transgenes from a variety of transgenic plant and algal species. Our protocol consists of homogenizing plant tissue with a pestle, boiling the homogenized tissue in a microfuge tube with 5% Chelex 100 for 5 min, and centrifuging the boiled mixture. The supernatant, which is used for PCR analysis, was able to successfully amplify transgenes in transgenic tobacco, tomato, potato, Arabidopsis, rice, strawberry, Spirodela polyrhiza, Chlamydomonas, and Porphyra tenera. The entire DNA extraction procedure requires <15 min and is therefore comparable to that used for bacteria, Chlamydomonas, and animal cell lines.

Transbronchial and Transesophageal Fine-Needle Aspiration Using an Ultrasound Bronchoscope in Mediastinal Staging of Potentially Operable Lung Cancer

Hwangbo, Bin,Lee, Geon-Kook,Lee, Hee Seok,Lim, Kun-Young,Lee, Soo-Hyun,Kim, Hyae-Young,Lee, Hyun Sung,Kim, Moon Soo,Lee, Jong Mog,Nam, Byung-Ho,Zo, Jae Ill Elsevier 2010 Chest Vol.138 No.4

수확시기, 예건 및 싸라기 처리가 유기 청보리의 사초 생산성 및 사일리지 품질에 미치는 영향

김종덕(Jong Duk Kim),이현진(Hyun Jin Lee),전경협(Kyeong Hup Jeon),양가영(Ga-Young Yang),권찬호(Chan Ho Kwon),성하균(Ha Guyn Sung),황보순(Soon Hwangbo),조익환(Ik Hwan Jo) 한국초지조사료학회 2010 한국초지조사료학회지 Vol.30 No.1

최근에 청보리 사일리지는 국내에서 가장 많이 이용하는 조사료이지만 유기축산을 위한 유기조사료의 생산과 품질에 관한 연구는 미흡한 실정이다. 따라서 본 시험은 유기 청보리 사일리지의 적정 수확시기를 구명하고, 사일리지 품질 향상을 위하여 예건과 싸라기를 첨가하여 사일리지를 평가하였다. 시험구 배치는 12처리 3반복의 분할구 배치법으로 주구는 수확시기로 출수기, 유숙기 및 황숙기를 두었으며, 세구는 무처리, 예건, 싸라기 10%, 싸라기 15%를 두었다. 수확시 건물함량은 출수기, 유숙기 및 황숙기가 각각 12.8%, 21.9% 및 29.8%로 증가하였다. 청보리의 건물수량은 출수기, 유숙기 및 황숙기가 각각 10,346, 15,819 및 18,336 ㎏/㏊였으며, TDN 수량은 6,288, 9,550 및 10,178㎏/㏊였다. 유기 사일리지의 pH는 출수기, 유숙기 및 황숙기가 각각 4.70, 4.03 및 5.37로 유숙기가 가장 낮았다. 청보리의 수확시기가 늦어짐에 따라 조단백질, 조지방, NFC 및 TDN 함량은 감소하였으나, NDF 및 ADF 함량은 증가하였다. 그러나 조회분 함량은 유숙기가 가장 낮았다. 사일리지의 예건과 싸라기의 첨가는 조회분, NDF 및 ADF 함량을 감소시키고, NFC와 TDN 함량은 증가시켰다. 사일리지의 건물소화율은 수확시기가 늦어짐에 따라 감소하였으며, 예건과 싸라기 첨가에 의하여 건물소화율이 증가하였다. 유숙기가 다른 수확시기보다 젖산과 총유기산 함량은 높고 낙산 함량이 낮아 총유기산 중 젖산함량이 가장 높았다. 예건과 싸라기 첨가에 의한 젖산 함량 증가 효과는 출수기가 다른 수확기보다 효과가 있었다. 이상의 결과를 종합해 볼 때 유기 청보리 사일리지 제조를 위한 수확적기는 유숙기 이었으며, 예건과 싸라기 첨가에 의하여 청보리 사일리지의 품질을 향상시킬 수 있었다. Although whole crop barley (Hordeum vulgare L.) is now widely grown as a silage crop in Korea, forage production and silage quality of that for organic farm have not been published. Therefore, this experiment was conducted to evaluate the effect of harvest stage on forage production and quality of organic barley, and effect of field wilting and crushed rice for shortening of harvest date and improvement of forage quality. The experiment was split-plot design with three replications. Main plots were heading, milking and yellow stages, and sub-plot were field wilting for 1 day, crushed rice 10% (CR10%) and 15% (CR15%) treatments. The dry matter (DM) contents of heading, milking and yellow stages were 12.8%, 21.9% and 29.8%, respectively. The DM yields of heading, milking and yellow stages were 10,346, 15,819 and 18,336 kg/ha, respectively, and the total digestible nutrients (TDN) of these were 6,288, 9,550 and 10,178 kg/ha, respectively. The pH of milking stage showed low 4.00 pH values. The crude protein, ether extract, crude ash, non-fiber carbohydrate (NFC) and TDN contents were decreased as harvest stage progressed, while neutral detergent fiber (NDF) and acid detergent fiber (ADF) were increased. The crude ash (CA) of milking stage showed the lowest among harvest stages. Field wilting and crushed rice treatments decreased CA, NDF and ADF contents, and increased NFC and TDN contents. In vitro dry matter digestibility (IVDMD) decreased with progressed harvest stage, while field wilting and crushed rice treatments increased that of barley silage. Lactic acid and total organic acid contents of milking stage were the highest, and butyric acid of milking stage was the lowest among harvest stage. The good effect of field wilting and crushed rice was observed in heading stage. The experiment results indicate that optimum harvest stage of barley silage for organic was milking stage. The field wilting and crushed rice additive could be recommended as effective method for shortening harvest date and increasing forage quality of organic barley silage.

ORIGINAL PAPER : Inhibitory effects of juglanin on cellular senescence in human dermal fibroblasts

( Hyo Hyun Yang ),( Kyong Hwangbo ),( Ming Shan Zhang ),( Jong Keun Son ),( Hwa Young Kim ),( Suk Hwan Baek ),( Hyung Chul Choi ),( So Young Park ),( Jae Ryong Kim ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Cellular senescence contributes to tissue and organismal aging, tumor suppression and progress, tissue repair and regeneration, and age-related diseases. Thus, aging intervention might be a promising target for treatment and prevention of diverse age-related diseases. In the present study, we investigated whether juglanin purified from the crude extract of Polygonum aviculare exerted inhibitory activity against cellular senescence in human dermal fibroblasts (HDFs). Juglanin decreased senescence-associated β-galactosidase activity (SA-β-gal) and the level of reactive oxygen species in senescent cells induced by adriamycin treatment. Juglanin also repressed SA-β-gal activity in HDFs under replicative senescence. These results suggest that juglanin represses cellular senescence in HDFs and might be useful for the development of dietary supplements or cosmetics that alleviate tissue aging or age-related diseases.