Interleukin-1β와 tumor necrosis factor-α가 마우스 조골세포의 nitric oxide 생성에 미치는 영향

채종성,고선일,김정근 단국대학교 치의학연구소 1996 논문집 Vol.8 No.1

Bone remodeling is characterized by the coupling of osteoclast-mediated bone resorption and osteoblast-mediated bone formation. The process is tightly regulated at the local level by an incompletely known complex network of humoral factors produced in the bone microenvironment, including peptide as well as non-peptide molecules. Nitric oxide(NO) is recently identified messenger molecule regulating a wide range of functions throughout the body but little is known about its possible role in skeletal metabolism. Many other cells, including macrophages, hepatocytes, chondrocytes and bone marrow cells, also produce NO. Recently, NO was reported to inhibit osteoclastic bone resorption in vitro, and there are indications of an arginine-dependent NO pathway in osteoblasts and osteoclasts. In this study we present evidence that mouse osteoblasts can be induced to produce NO by cytokines (interleukin-1β (IL-1β), tumor necrosis factor-α (TNT-α) known to modulate bone cell activity. The observed results were as follows. 1. In case of 24 hr incubation with IL-1β, NO production of MC3T3/E1 cells was not stimulated. But significant amounts of NO were measured after 48 hr incubation. In case of TNT-α, significant amounts of NO were measured after 24 hr, and increased further during the next 48 hr followed by a much slower increase up to 96 hrs. 2. IL-1β stimulated NO production into cultured medium in cultures of MC3T3/E1 cells dose dependently (0.4, 0.2, 1 ng/ml). 3. TNT-α stimulated NO production into cultured medium in cultures of MC3T3/E1 cells dose dependently (0.2, 1, 5 ng/ml). 4. Cytokine combination increased NO production dose dependently. And synergistic effects of the two cytokines were observed at all dose combinations.

수종의 cytokine이 배양중인 조골세포에 미치는 영향

李鍾烈,高鮮一,金正根 단국대학교 치의학연구소 1995 논문집 Vol.7 No.1

Evidence indicates that cytokines are involved in the initiation and progression of chronic inflammatory diseases. Recent evidence demonstrates major roles of growth factors and cytokines as mediators for bone growth and remodeling. Bone remodeling is a local phenomenon which occurs in discrete packets throughout the skeleton. The cellular events which comprise the remodeling sequence are controlled by cytokines which are generated in the microenvironment of the bone resorbing area. These cytokines are derived from marrow mononuclear cells or from bone cells themselves, or they are incorporated into the bone matrix and released in biologically active form as bone resorb. But evidence is accumulating that some of these cytokines play an important role not just in physiological bone remodeling, but also in common diseases of bone remodeling such as osteoporosis, osteopetrosis, Paget's diseases and malignant diseases which involve bone and chronic inflammatory diseases such as rheumatoid arthritis and periodontal diseases. Also, these cytokines are derived from bone cells themselves, these receptors are present in bone cells. Therefore, this experiment developed a culture of osteoblastic cells from embryonic chicken calvaria to examine the possible role of some cytokine in osteoblast function. This experiment was performed 1) to examine the effect of cytokines on the acid phosphatase activity of chicken osteoblast and 2) to examine the effect of cytokines on the alkaline phosphatase activity of chicken osteoblast and 3) to study the effect of cytokines on the bone nodule formation of chicken osteoblast in long-term cultures. The observed results were as follows. 1. Cytokines and cytokine combination stimulate the release of acid phosphatase into cultured medium in cultures of chicken osteoblast. 2. Cytokines and cytokine combination increase the activity of acid phosphatase of cell extract in cultures of chicken osteoblast. 3. Cytokines and cytokine combination decrease the activity of alkaline phosphatase of cell extract in cultures of chicken osteoblast. 4. Less bone nodules were formed by cytokines than in control group.

수종의 cytokine이 배양중인 조골세포에 미치는 영향

李鐘烈,高鮮一,金正根 단국대학교 치의학연구소 1995 단국대치의학연구소논문집 Vol.7 No.1

콜레스테롤 생합성 저해제 검색법의 개발

임종철,이남훈,김한균,염형렬,이대호,강현석,류형선,명재일,김완,신부안 全南大醫大 2001 전남의대학술지 Vol.37 No.2

옥수수 種實 形質의 遺傳分析

申昌浩,鄭丞根,車善佑 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1

Five inbreds and 20 F1s form a 5×5 diallel cross were used to obtain genetic informations on kernel characters in corn, which are necessary to improve seeds more adaptable to mechanical planter and to produce higher yielding hybrids with greater kernel dimension. Both general combining ability (GCA) and specific combining ability (SCA) were significant for kernel length (L), kernel width (W), kernel thickness (T), kernel weight, L/W, L/T and W/T. Greater GCA mean squares indicated additive effects were more important for W and L/W, while non-additive effects were more important for other characteristics. Significant reciprocal effects showed that female parents had greater effect on kernel dimension. Heterosis and heterobeltiosis were 14-43% and 8-31%, respectively, for all characteristics except T. GCA effects indicated that inbreds FR25 and KS42 increased L, KI16A increased W and WT, FR14A increased T, while FR2A decresed L, W, T, and WT. Genetic analysis also indicated the dominance and additive effect for W/T. No interactions between non-allelic genes and overdoinance were indicated for L, W and WT. Narrow sense heritability (0.29) indicated that selection for only W would be effective, although broad sense heritabilities for L, W and WT were 0.78-0.87

초내열 합금 Alloy718의 저주기 피로시험에서 반복연화 및 R=X (X=0,-1)효과

최병학,신수근,신정근,김선화,윤기봉,염종택,박노광 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

The LCF properties for R=0 as tension-tension and R=-1 as tension-compression were examined in DA alloy718. While the hysteresis loops composed of ?ε_(t), ?ε_(p) and ?σ were figured with similaity in both of R=0 and R=-1, the loops of R=0 were continuely going down along the stress axis during cyclic fatigue progressing. It was caused by Bauschinger effect, which includes a tendancy for stress recovering as an amount of compression stress. The phenomena of cyclic softening in both of R=0 and R=1 seem to be caused by planar slip of dislocations, which was accompanied by shearing the γ'/γ" precipitates during the cyclic fatigue test.

Case Reports : Dermatofibrosarcoma Protuberans Arising from a Burn Scar

Jong Keun Seo,Kyung Jong Cho,Ju Hyun Kang,Deborah Lee,Ho Suk Sung,Seon Wook Hwang 대한피부과학회 2009 Annals of Dermatology Vol.21 No.4

Malignant neoplasms arising in burn scars are well known. In previous literature, 25 cases of burn scar sarcomas were reported. However, dermatofibrosarcoma protuberans is very rare and only two cases have been reported. A 43-year-old Korean man presented with multiple erythematous clustered plaques and nodules and a skin-colored subcutaneous mass on the chest after a severe burn injury at the age of 8 years. A biopsy specimen revealed dermatofibrosarcoma protuberans. The tumor was excised widely to include the surrounding burn scar. Herein, we report this third case of dermatofibrosarcoma protuberans arising from a burn scar. (Ann Dermatol 21(4) 416~418, 2009)

The Accuracy of Lower Extremity Alignment in a Total Knee Arthroplasty Using Computer-Assisted Navigation System

Jong Keun Seon(선종근),Eun Kyoo Song(송은규) 대한정형외과학회 2004 대한정형외과학회지 Vol.39 No.5

목적: Computer-assisted navigation system을 이용한 인공 슬관절 치환술의 하지 정열 및 내고정물 위치의 정확도를 평가하고자 하였다. 대상 및 방법: 고식적 방식(manual alignment system)을 이용한 40예(A군)와 navigation system을 이용한 36예(B군)를 방사선학적으로 비교 분석하였다. 수술 후 2개월에 시행한 방사선 촬영에서 대퇴 경골각, 기계적인 축, 대퇴 및 경골 삽입물의 경사도를 정면 및 측면 사진에서 측정하였다. 결과 평가는 적정위치±3° 이내를‘양호(acceptable)’, 적정위치±3° 초과를‘불량(outlier)’으로 하였다. 결과: 관상면에서 대퇴 삽입물의 경사도 측정시, 대퇴골의 해부학적 축과 이루는 각은 A군에서 불량이 13예와 B군에서 1예이었다. 대퇴골의 기계적인 축과 이루는 각은 A군에서 17예, B군에서 2예이었다. 관상면에서 경골 삽입물의 경사도와 시상면에서 대퇴 삽입물의 경사도는 두 군간의 차이가 없었다. 시상면에서 경골 삽입물의 경사도는 A군에서 16예, B군에서 0예이었다. 대퇴 경골각의 불량은 두군에서 각각 9예, 2예이었다. 체중 부하 전후면 사진에서 기계적인 축의 변위에 있어 불량은 각각 9예, 2예로 navigation군에서 적은 오차가 관찰되었다. 결론: Navigation system을 이용한 인공 슬관절 치환술은 기존방식에 비해‘양호’결과가 훨씬 많았으며, 불량은 적었다. 따라서 navigation system을 이용한 인공 슬관절 치환술은 정확한 하지 정렬을 얻을 수 있었으며, 인공 슬관절 치환술에 유용한 것으로 사료된다. Purpose: This study assessed the accuracy of the alignment of the lower extremity and the position of the components in a total knee arthroplasty (TKA), which was performed using a computer-assisted navigation system. Materials and Methods: This study reviewed 40 TKA using a manual alignment system (group A) and 36 TKA using the Orthopilot<SUP>®</SUP> 4.0 navigation system (group B). The radiographs taken 2 months after surgery were used to measure the femoro-tibial angle, the mechanical axis deviation, and the inclination of the femoral and tibial component. A value within the optimum±3° was defined as an ‘acceptable’ and a value over the optimum±3° was befined as an ‘outlier’. Results: The coronal inclination of the femoral component to the anatomical femoral axis showed outliers in 13 knees in group A and 1 knee in group B. The coronal inclination of the femoral component to the mechanical axis revealed 17 and 2 outliers in each group, respectively. The coronal inclination of the tibial component and sagittal inclination of femoral component showed no significant differences between two groups, but the sagittal inclination of tibial component showed 16 and no outlier in each group, respectively. Concerning the femoro-tibial angle, there were 9 outliers in group A and 2 outliers in group B. The mechanical axis deviation showed 9 and 2 outliers in groups A and B, respectively. Conclusion: The navigation system helped to produce more ‘acceptable’ and less ‘outlier’ results than the manual alignment system. The alignment of the lower extremity after a TKA can be achieved more accurately using the navigation system.

인공 슬관절 반치환술의 방사선학적 비교

선종근(Jong-Keun Seon),송은규(Eun-Kyoo Song),윤택림(Taek-Rim Yoon),배봉현(Bong-Hyun Bae),김철영(Cheol-Young Kim) 대한정형외과학회 2006 대한정형외과학회지 Vol.41 No.1

목적: 인공 슬관절 반치환술을 시행받은 환자 중 고식적 방법과 내비게이션을 이용한 군의 방사선학적 결과를 비교 분석하였다. 대상 및 방법: 인공 슬관절 반치환술을 시행받은 46명의 환자 50예를 대상으로 하였다. 고식적 방법을 이용한 경우는 22명 25예였으며, 내비게이션을 이용한 경우는 24명 25예였다. 술 전 및 후의 슬관절 전후면 및 측면 방사선 사진과 하지 전장을 포함하는 체중부하 전후면 사진을 비교하였다. 결과: 술 후 대퇴-경골각은 고식적 방법을 이용한 군에서 외반 3.6도로, 내비게이션을 사용한 군에서 외반 5.4도로 교정되었다. Kennedy와 White의 방법으로 측정한 역학적 축의 위치는 고식적 방법을 이용한 군은 술 후 구역 1은 4예(16%), 구역 2 또는 C에 21예(84%)가 위치하였다. 내비게이션을 이용한 군은 술 후 전 예에서 구역 C에 위치하였다 (p=0.001). 관상면의 대퇴골 삽입물은, 고식적 방법을 이용한 경우 우수 11예(44%), 양호 7예(28%), 불량 7예(28%)인 반면, 내비게이션을 이용한 경우 우수 15예(60%), 양호 10예(40%)였으며 불량은 없었다(p<0.05). 경골 삽입물은 관상 면에서 고식적 방법을 이용한 경우 우수 22예(88%), 불량 3예(12%)였으며, 내비게이션을 이용한 경우 전 예에서 우수한 결과를 보였고(p>0.05) 시상면에서 고식적 방법을 이용한 경우 우수 4예(16%), 양호 12예(48%), 불량 9예(36%)였으며, 내비게이션을 이용한 경우 우수 20예(80%), 양호 5예(20%)였으며 불량은 없었다(p<0.05). 결론: 내비게이션을 이용한 인공 슬관절 반치환술은 고식적인 방법에 비해 정확한 하지의 해부학적 축과 삽입물의 정렬을 회복하는데 더욱 좋은 결과를 보여 향후 인공 슬관절 반치환술에 유용하게 이용할 수 있을 것으로 생각된다. Purpose: To compare the radiographic results of unicompartmental knee arthroplasty using a navigation system with those using a manual alignment system. Materials and Methods: The results of 50 cases (46 patients) of unicompartmental knee arthroplasty using a navigation system (24 patients, 25 cases) and using a manual alignment system (22 patients, 25 cases) were evaluated. Knee anteroposterior, lateral, and weight-bearing full-length lower extremity radiographs before and after the arthroplasty were used for the measurements. Results: The mean postoperative femorotibial alignment was 3.60 of valgus in the manual alignment group and 5.4˚ of valgus in the navigation group. The postoperative mechanical axis measured using Kennedy and White's method was located at zone 1 in 4 cases (16%), at zone 2 or C in 21 cases (84%) in the manual group, and at zone C for all cases in the navigation group (p=0.001). In the coronal axis of the femoral component, 11 cases (44%) were excellent, 7 cases (28%) were good and, 7 cases (28%) were poor in the manual alignment group. In the navigation group, 15 cases (60%) were excellent and 10 cases (40%) were good and there were no poor results (p<0.05). In the coronal axis of the tibial component, 22 cases (88%) showed excellent results and 3 cases (12%) showed poor results in the manual alignment group. In the navigation group, all cases were showed excellent results (p>0.05). In the sagittal axis of the tibial component, 9 cases (36%) showed poor results in manual group and there were no poor results in the navigation group (p<0.05). Conclusion: Unicompartmental knee arthroplasty using navigation produced better results in restoring the alignment of the prosthesis and the mechanical axis of the lower extremity than that using manual alignment.

항법 장치 시스템을 이용한 개방형 근위 경골 절골술

선종근(Jong-Keun Seon),김하성(Ha-Sung Kim),김도연(Do-Youn Kim),송은규(Eun-Kyoo Song) 대한정형외과학회 2014 대한정형외과학회지 Vol.49 No.2

퇴행성 관절염에서 개방형 근위 경골 절골술은 고정기구 및 수술 기법의 발달로 많이 시행되고 있다. 또한 과거엔 고식적인 방법으로 술자의 경험에 의존하여 기계적 축을 변화시켰지만 최근 수술 술기 및 과학 기술의 발달로 항법 장치 시스템을 이용하여 술자가 원하 는 각도로 기계적 축을 교정함으로써 수술의 정확성 및 정밀성 향상과 함께 좋은 임상 결과가 보고되고 있다. 또한 이러한 항법 장치 시스템은 실시간 모니터링을 통해 수술 중 하지 축 정렬을 수술자가 원하는 대로 교정할 수 있을 뿐만 아니라 방사선 노출 시간도 현 저히 감소 시킬 수 있다. 하지만 고식적 방법에 비해 수술 시간 연장 및 추척자(tracker) 사용에 따른 추가적 절개와 그로 인한 감염이 나 골절의 가능성도 있다. 또한, 체중 부하 하지 전장 방사선 사진에서 얻은 하지의 기계적 축과 체중부하를 하지 않은 항법 장치 시 스템을 이용한 술전 하지의 기계적 축 사이에는 차이가 발생할 수 있으며 등록 오류나 기계적 결함 등 기술적인 함정이 발생할 수 있 다는 단점 또한 지니고 있다. 이번 종설에서는 항법 장치 시스템을 이용한 개방형 근위 경골 절골술의 소개, 수술 방법 및 임상적용, 수술 시 주의할 사항 및 경골 후방 경사각 조절, 그리고 임상결과에 대해 알아보도록 하겠다. Navigation systems are currently being widely used in orthopedic surgery. The mechanical axis alignment can be judged accurately via a navigation system. High tibial osteotomy (HTO) is a procedure that aims to change the mechanical axis of the lower limb, transferring the body weight across healthy articular cartilage. Several studies have shown that accurate correction is the leading predictor for success. And, by using a navigation system, accurate multiplane measurements of the lower limb alignment can be performed intraoperatively in real time, and alignment adjustments can be made as the surgeon desires. Compared with the conventional cable-method, computer navigation significantly improves the accuracy of postoperative leg axis, reduces correction variability with fewer outliers, and reduces radiation time. This paper reviews the advantages, clinical results, complications, pitfalls, and posterior tibial slope control in navigation guided open wedge HTO.