B형 간염 바이러스 단백질에 있어서 HLA-A2에 의해 표현되는 Epitope 펩타이드 들의 분석

이희구,임종석,김승목,이기영,김희수,김승호,권태종,최인성,정태화,김길현 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

The cytotoxic T lymphocyte (CTL) are an important component in host defense mechanism against viral infection. They can recongnize virus-derived peptides presented by the Class I MHC molecule at the cell surface of the infected cells. On searching for effective CTL epitopes of hepatitis B virus(HBV), we synthesized a distinct set of 9-10 mer peptide containing amino acid sequence of hepatitis B virus surface protein that are selected on the basis of a computer modeling and the previously described HLA-A2 specific motifs.Binding assay of the synthetic peptides to HLA-A2 molecules using human antigen processing defectant T2 cells showed that 3 out of 4 synthetic peptides enhanced the expression of HLA-A2 molecule on T2 cell surface.Two anchor positions, namely P2 and P9(or P10) appeared to play a decisive role for binding.Structural. characteristics of the peptides addressed by molecular dynamics simulation was analysed and compared.These peptides also partially triggered CTL isolated from human peripheral blood mononuclear cells of HBV positive patients, and the response was peptide-spcific.These results showed that negatively-charged amino acid residue at P2 hampered binding affinity of the peptides to HLA-A2 molecules, and that binding affinity of the peptides are not always reflected by their immunogenicity among natural T cell repertoire.

B형 간염 바이러스 단백질에 있어서 HLA-A2에 의해 표현되는 Epitope 펩타이드 들의 분석

이희구,임종석,김승목,이기영,김희수,김승호,권태종,최인성,정태화,김길현 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

The cytotoxic T lymphocyte(CTL) are an important component in host defense mechanism against viral infection. They can recongnize virus-derived peptides presented by the ClassⅠ MHC molecule at the cell surface of the infected cells. On searching for effective CTL epitopes of hepatitis B virus(HBV), we synthesized a distinct set of 9-10 mer peptide containing amino acid sequence of hepatitis B virus surface proteion that are selected on the basis of a computer modeling and the previously described HLA-A2 specific motifs. Binding assay of the synthetic peptides to HLA-A2 molecules using human antigen processing defectantn T2 cells showed what 3 out of 4 synthetic peptides enhaced the expression of HLA-A2 molemule on T2 cell surface. Two anchor positions, namely P2 and P9(or P10) appeared to play a decisive role for binding. Structural chacteristics of the peptides addressed by molecular dynamics simulation was analysed and compared. These peptides also parially triggerd CTL isolatied frmo human peripheral blood mononuclear cells of HBV positive patients, and the response was peptide-specific. These results showed that negatively-charged amino acid residue at P2 hampered binding affinity of the peptides to HLA-A2 molecules, and that binding affinity of the peptides are not always reflected by thier immunogenicity among natural T cell repertoire.

담즙정체성 간염의 임상적 양상

최선택,은종렬,임상우,김봉준,이헌주,구미진,최준혁 영남대학교 기초/임상의학연구소 2001 Yeungnam University Journal of Medicine Vol.18 No.1

Background: Cholestatic hepatitis is failure of bile to reach the duodenum with hepatocellular damage and no demonstrable obstruction of the major bile ducts. The prognosis is usually good with recovery in less than 4 weeks after withdrawal of the offending drug. However, a prolonged course lasting over 3 months is possible and, in rare cases, progression to ductopenia with development of a vanishing bile duct syndrome occurs. A differential diagnosis with other causes of Chronic liver disease is needed. Materials and Methods: From January 1991 through Jaunary 2000, 14 patients diagnosed as cholestatic hepatitis by liver biopsy were inclouded. The possible causative drug, clinical features, laboatory findings, and progression of cholestatic hepatitis were evaluated. The semiquantitative study of liver lesions was performed by two independent observers. Results: Causes of cholestatic hepatitis are 5 cases of oriental medicine, 3 cases of anti-tuberculosis medication, 1 case of ticlopidine and antibiotics and 4 cases of unknown causes. The clinical features of cholestatic hepatitis were jaundice, itching, urine color change, and general weakness. During 6 to 30 months, LFT of 5 patients showed prolonged elevation. Elevated total cholesterol ≥250 mg/dL in 6 patients, pheripheral blood eosinophilia in 5 patients, auto-antibody positive in 6 patients were observed respectively. The biopsies showed intralobular bilirubinostasis with a mixed portal inflammatory infiltration. Conclusion: In cholestatic hepatitis. durations of abnormal LFT are variable regardless of causative drugs. If cholestatic hepatitis progresses toward chronic course, viral hepatitis, primary biliary cirrhosis, and autoimmune hepatitis should be differentially diagnosed and sequential liver biopsies are needed.

NDRG2 is one of novel intrinsic factors for regulation of lL-10 production in human myeloid cell

최승철,김광동,김종태,오상석,윤선영,송은영,이희구,조영경,최인표,임종석,김재화 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

NDRG2 is one of novel intrinsic factors for regulation of lL-10 production in human myeloid cell

최승철,김광동,김종태,오상석,윤선영,송은영,이희구,조영경,최인표,임종석,김재화 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

Mycobacterium intracellulare Pleurisy Identified on Liquid Cultures of the Pleural Fluid and Pleural Biopsy

Jong Gu Lim,Sei Won O,Ki Dong Lee,Dong Keun Suk,Tae Young Jung,Tae Sun Shim,Gyu Rak Chon 대한결핵 및 호흡기학회 2013 Tuberculosis and Respiratory Diseases Vol.74 No.3

Pleural effusion is a rare complication in non-tuberculous mycobacterial infection. We report a case of Mycobacterium intracellulare pleuritis with idiopathic pulmonary fibrosis in a 69-year-old man presenting with dyspnea. Pleural effusion revealed lymphocyte dominant exudate. M. intracellulare was identified using a polymerase chain reaction-restriction fragment length polymorphism method and liquid cultures of pleural effusion and pleural biopsy. After combination therapy for M. intracellulare pulmonary disease, the patient was clinically well at a 1- month follow-up.

Treatment Modification Is not Needed for Early Alanine Aminotrasferase Flare in Treatment-naive Patients with Chronic Hepatitis B Initiated on Tenofovir

( Jong Gu Lim ),( Jin Yong Kim ),( Jeong Rok Lee ),( Joon Ho Wang ),( Jeong Han Kim ),( Won Hyeok Choe ),( So Young Kwon ),( Soon Young Ko ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Tenofovir disoproxil fumarate (TDF) is a potent antiviral drug used in the treatment of patients with chronic hepatitis B (CHB). The aim of this study was to evaluate the antiviral efficacy and safety of continuous TDF monotherapy after early alanine aminotrasferase (ALT) flare in treatment-naive patients initiated on TDF. Methods: A total of 40 treatment-naive CHB patients were treated with a 300-mg once-daily dose of TDF for more than 12 weeks. Virological markers of hepatitis B virus (HBV) and biochemical data were monitored at baseline and every 1-3 months during the therapy. The proportion of patients with undetectable HBV DNA level (< 1.25 log10 IU/ml) was noted. Results: At the baseline, median age was 44 years, and there were 31 male subjects, and 33 HBeAg-positive subjects; there was no cases of cirrhosis. Median follow-up was 36 weeks (12-140 weeks). Baseline mean HBV DNA levels were 6.04 ± 2.3 log10 IU/mL. Baseline mean ALT levels were 157 ± 144 IU/mL. Serum HBV DNA was undetectable in 58.3% and 100% of the patients at weeks 48 and 96. HBeAg loss was observed in 2 patients during the treatment period. Two HBeAg-positive patients (5%) showed ALT flares (>10 × upper limit of the normal range), without viral breakthrough, HBeAg loss, or seroconversion within first 4 weeks after the start of TDF monotherapy. ALT flares resolved within 4 weeks and both patients showed virologic response without interruption or discontinuation of treatment. Among baseline factors, young age (≤ 40 years) was predictive of early ALT flare (p = 0.048). Conclusions: Continuous TDF monotherapy may be effective and safe in treatment-naive patients with CHB experiencing early ALT flares without viral breakthrough. Early ALT flares may be related to young age in treatment-naive patients with CHB started on TDF.

SELECTION OF PEPTIDES THAT BIND TO THE HLA-A2.1 MOLECULE BY MOLECULAR MODELLING

LIM, JONG-SEOK,KIM, SEUNGMOAK,LEE, HEE GU,LEE, KI-YOUNG,KWON, TAE-JONG,KIM, KILHYOUN 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

Cytotoxic T lymphocytes recognize antigenic peptides in association with major his-tocompatibility complex class I proteins. Although a large set of class I binding peptides has been described, it is not yet easy to search for potentially antigenic peptides without synthesis of a panel of peptides, and subsequent binding assays. In order to predict HLA-A2.1-restricted antigenic epitopes, a computer model of the HLA-A2.1 molecule was established using X-ray crystallography data. In this model nonameric peptide sequences were aligned. In a molecular dynamics(MD)simulation with two sets of peptides known to be presented by HLA-A2.1, it was important to know the anchor amino acid residue preference and the distance between the anchor residues. We show here that the peptides bound to the HLA-A2.1 model structure possess a side chain of C-terminal anchor residue oriented into the binding groove with different distances between the two anchor residues from 15 to 21Å. We also synthesized a set of nonamer peptides containing amino acid sequences of Hepatitis B virus protein that were selected on the basis of previously described HLA-A2.1 specific motifs. When results obtained from the MD simulation were compared with functional binding assays using the TAP-deficient cell line T2, it was evident that the MD simulation method improves prediction of the HLA-A2.1 binding epitope sequence. These results suggest that this approach can provide a way to predict peptide epitopes and search for antigenic regions in sequences in a variety of antigens without screening a large number of synthetic peptides.

SELECTION OF PEPTIDES THAT BIND TH THE HLA-A2.1 MOLECULE BY MOLECULAR MODELLING

LIM, JONG-SEOK,KIM, SEUNGMOAK,LEE, HEE GU,LEE, KI-YOUNG,KWON, TAE-JONG,KIM, KILHYOUN 梨花女子大學校 藥學硏究所 1997 藥學硏究論文集 Vol.- No.6

Cytotoxic T lymphocytes recognize antigenic peptides in association with major histocompatibility complex class I proteins. Although a large set of class I binding peptides has been described, it is not yet easy to search for potentially antigenic peptides without synthesis of a panel of peptides, and subsequent binding assays. In order to predict HLA-A2.1-restricted antigenic epitopes, a computer model of the HLA-A2.1 molecule was established using X-ray crystallography data. In this model nonameric peptide sequences were aligned. In a molecular dynamics (MD) simulation with two sets of peptides known to be presented by HLA-A2.1, it was important to know the anchor amino acid residue preference and the distance between the anchor residues. We show here that the peptides bound to the HLA-A2.1 model structure possess a side chain of C-terminal anchor residue oriented into the binding groove with different distances between the two anchor residues from 15 to 21A. We also synthesized a set of nonamer peptides containing amino acid sequences of Hepatitis B virus protein that were selected on the basis of previously described HLA-A2.1 specific motifs. When results obtained from the MD simulation were compared with functional binding assays using the TAP-deficient cell line T2, it was evident that the MD simulation method improves prediction of the HLA-A2.1 binding epitope sequence. These results suggest that this approach can provide a way to predict peptide epitopes and search for antigenic regions in sequences in a variety of antigens without screening a large number of synthetic peptides.

In vitro Formation of Tuberous Roots from Root Ends in the Rooted Tuberous stem without shoots in Cyclamen persicum MILL.

Lim Jong-Gu,Junzo Fujigaki 한국자원식물학회 2004 Plant Resources Vol.7 No.3

In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL^-1 NAA, 0.5mg L^-1 BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.