Clock Self-Synchronization Protocol based on Distributed Diffusion for Wireless Sensor Networks

Min Li,Guoqiang Zheng,Jishun Li 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.5

A Virtual MIMO Communication Strategy Based on Cooperative Groups for Wireless Sensor Networks

Bing Li,Guoqiang Zheng,Na Li,Jishun Li 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.3

As we all know, virtual MIMO technology is an efficient way for energy saving in wireless sensor networks (WSNs). The present virtual MIMO communication strategies in WSNs depend on cluster heads collecting and forwarding source data, which causes big energy consumption. Aiming at this problem, we propose a new virtual-MIMO communication strategy based on cooperative group (CG) in this paper, called Group Collaboration MIMO (GCMIMO). GCMIMO clusters the WSN into several clusters. Each cluster is managed by two cluster heads, master cluster head (MCH) and vice cluster head (VCH). MCH divides the source nodes into several cooperative groups, every two nodes as a group. A CG can constitute a virtual dual-antenna transmitter for sending source data of both nodes directly. VCH and MCH are always seen as a CG, called master cooperative group (MCG), which can receive and forward the data from CGs of other clusters. Unlike the existing virtual MIMO communication strategy, GCMIMO doesn’t require the cluster heads collecting the data of the source nodes, which can reduce the times of source data transmission and improve the energy efficiency. In the simulation, we analyze the energy consumption in each communication phase of GCMIMO. The results indicate that GCMIMO can effectively decrease the energy consumption in data transmission and improve energy efficiency.

An Energy-efficient Transmission Scheme for Wireless Sensor Network with DSC and virtual MIMO

Guoqiang Zheng,Fangge Nie,Bing Li,Huahong Ma,Jishun Li,Yujun Xue,Peipei Li 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.10

Energy efficiency is one of the primary performance parametersin wireless sensor network (WSN). The data collected by neighboring nodesin the high density WSN tend to have a high correlation which isdirectly transmitted to the sink node (DGN) will seriously reduce the energy efficiency of wireless sensor networks.For improving the energy efficiency of high node density WSN, We introduce distributed source coding (DSC) which can compress the high correlation source data into WSNbased on virtual MIMO. In the simulationresults indicate thatDSC can effectively reduce the energy consumption in data transmission and improve the energy efficiencyof the whole WSN.

Trichoderma biodiversity in major ecological systems of China

Kai Dou,Jinxin Gao,Chulong Zhang,Hetong Yang,Xiliang Jiang,Jishun Li,Yaqian Li,Wei Wang,Hongquan Xian,Shigui Li,Yan Liu,Jindong Hu,Jie Chen 한국미생물학회 2019 The journal of microbiology Vol.57 No.8

An investigation of Trichoderma biodiversity involving a large-scale environmental gradient was conducted to understand the Trichoderma distribution in China. A total of 3,999 isolates were isolated from forestry, grassland, wetland and agriculture ecosystems, and 50 species were identified based on morphological characteristics and sequence analysis of genetic markers. Trichoderma harzianum showed the largest proportion of isolates and the most extensive distribution. Hypocrea semiorbis, T. epimyces, T. konilangbra, T. piluliferum, T. pleurotum, T. pubescens, T. strictipilis, T. hunua, T. oblongisporum and an unidentified species, Trichoderma sp. MA 3642, were first reported in China. Most Trichoderma species were distributed in Jilin and Heilongjiang Provinces in northeast China and the fewest were distributed in Qinghai Province. Based on the division of ecological and geographic factors, forestry ecosystems and low-altitude regions have the greatest species biodiversity of Trichoderma.

Self-assembled Viral Nanoparticles as Targeted Anticancer Vehicles

Yuanzheng Wu,Jishun Li,신현재 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.1

Viral nanoparticles (VNPs) comprise a variety of mammalian viruses, plant viruses, and bacteriophages, that have been adopted as building blocks and supramolecular templates in nanotechnology. VNPs demonstrate the dynamic, monodisperse, polyvalent, and symmetrical architectures which represent examples of such biological templates. These programmable scaffolds have been exploited for genetic and chemical manipulation for displaying of targeted moieties together with encapsulation of various payloads for diagnosis or therapeutic intervention. The drug delivery system based on VNPs offer diverse advantages over synthetic nanoparticles, including biocompatibility, biodegradability, water solubility, and high uptake capability. Here we summarize the recent progress of VNPs especially as targeted anticancer vehicles from the encapsulation and surface modification mechanisms, involved viruses and VNPs, to their application potentials.

Fungal and mushroom hydrophobins: A review

Wu, Yuanzheng,Li, Jishun,Yang, Hetong,Shin, Hyun-Jae The Korean Society of Mushroom Science 2017 한국버섯학회지 Vol.15 No.1

Hydrophobins are surface active proteins that are produced by filamentous fungi including mushrooms. Their ability to self-assemble into an amphipathic membrane at any hydrophilic-hydrophobic interface is most intriguing. These small secreted proteins comprise of eight conserved cysteine residues which form four disulfide bridges and an extraordinary hydrophobic patch. Hydrophobins play critical roles in fungal (and/or mushrooms) growth as structural components and in the interaction of fungi and mushrooms with the environment. The biophysical and biochemical properties of the isolated proteins are remarkable, such as strong adhesion, high surface activity and the formation of various self-assembled structures. With the increasing demands of hydrophobins from fungi and mushroom sources, production and purification in large scale is under challenge. Various applications, ranging from food industries, cosmetics, nanotechnology, biosensors and electrodes, to biomaterials and pharmaceuticals are emerging and a bright future is foreseen.

Fungal and mushroom hydrophobins: A review

Yuanzheng Wu,Jishun Li,Hetong Yang,Hyun-Jae Shin 한국버섯학회 2017 한국버섯학회지 Vol.15 No.1

Hydrophobins are surface active proteins that are produced by filamentous fungi including mushrooms. Their ability to self-assemble into an amphipathic membrane at any hydrophilic–hydrophobic interface is most intriguing. These small secreted proteins comprise of eight conserved cysteine residues which form four disulfide bridges and an extraordinary hydrophobic patch. Hydrophobins play critical roles in fungal (and/or mushrooms) growth as structural components and in the interaction of fungi and mushrooms with the environment. The biophysical and biochemical properties of the isolated proteins are remarkable, such as strong adhesion, high surface activity and the formation of various self-assembled structures. With the increasing demands of hydrophobins from fungi and mushroom sources, production and purification in large scale is under challenge. Various applications, ranging from food industries, cosmetics, nanotechnology, biosensors and electrodes, to biomaterials and pharmaceuticals are emerging and a bright future is foreseen.

Targeted Cowpea Chlorotic Mottle Virus-based Nanoparticles with Tumor-homing Peptide F3 for Photothermal Therap

Yuanzheng Wu,Jishun Li,Hetong Yang,성지현,임호동,김근중,신현재 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.6

Our aim was to devise targeted drug delivery systems using genetically modified cowpea chlorotic mottle virus (CCMV) capsids by fusion expression with tumorhoming peptide F3 for efficient delivery of therapeutic substances into tumor cells. The RNA-binding domain at the N terminus (amino acid residues 1–25) of CCMV capsid protein (CP) was selectively deleted, and F3 was inserted for the expression in Pichia pastoris. After chromatographic purification, F3-CCMV capsids were obtained via selfassembly of the F3-CP fusion protein and then analyzed by transmission electron microscopy and dynamic light scattering analysis, which revealed spherical nanoparticles (NPs) ca. 18 nm in diameter with regular monodispersity. Near-infrared fluorescent dye IR780 iodide, which has been applied for cancer imaging, photodynamic therapy, and photothermal therapy, was encapsulated in F3-CCMV NPs. The resultant F3-CCMV-IR780 NPs showed excellent molecular targeting to nucleolin receptor overexpressed on the surface of MCF-7 tumor cells. Furthermore, the in vitro cellular uptake and cell viability assay proved a photothermal effect by a single dose of near-infrared laser irradiation. The present system may offer a programmable nanoscaffoldbased drug delivery system vehicle for fabrication of promising therapeutic substances for cancer therapy.

A Large-scale Multi-track Mobile Data Collection Mechanism for Wireless Sensor Networks

( Guoqiang Zheng ),( Lei Fu ),( Jishun Li ),( Ming Li ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.3

Recent researches reveal that great benefit can be achieved for data gathering in wireless sensor networks(WSNs) by employing mobile data collectors. In order to balance the energy consumption at sensor nodes and prolong the network lifetime, a multi-track large-scale mobile data collection mechanism (MTDCM) is proposed in this paper. MTDCM is composed of two phases: the Energy-balance Phase and the Data Collection Phase. In this mechanism, the energy-balance trajectories, the sleep-wakeup strategy and the data collection algorithm are determined. Theoretical analysis and performance simulations indicate that MTDCM is an energy efficient mechanism. It has prominent features on balancing the energy consumption and prolonging the network lifetime.

Surface Modification of Cowpea Chlorotic Mottle Virus Capsids via a Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC) Reaction and Their Adhesion Behavior with HeLa Cells

Yuanzheng Wu,Hetong Yang,전영진,이민영,Jishun Li,신현재 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4

A copper(I)-catalyzed azide-alkyne cycloaddition(CuAAC) reaction was exploited for the surface modificationof cowpea chlorotic mottle virus (CCMV). The exposedcarboxyl residues of the CCMV capsids were modifiedwith an alkyne and then further modified with an azide,using a triazole connection in the presence of CuSO4,tris(2-carboxyethyl)phosphine hydrochloride (TCEP), anda bathocuproin disulfonic acid disodium salt (BCDS). Fluorogenic coumarin was successfully grafted onto theCCMV capsids and monitored by fast protein liquidchromatography (FPLC) and UV-irradiated SDS-PAGE. An oligo-ethylene glycol (OEG) short chain and an Arg-Gly-Asp (RGD) peptide were also connected to the CCMVcapsids via the CuAAC reaction. Size-exclusion FPLC,transmission electron microscopy (TEM), and dynamiclight scattering (DLS) analyses confirmed the modificationand integrity of the viral capsids. Interestingly, OEG-CCMVdisplayed a unique phenomenon of connected bridges withthe intact capsids crosslinked to each other. Coumarin-CCMV, OEG-CCMV, and RGD-CCMV were absorbedonto APTES slides for cell binding with HeLa cells. Theopposite adhesion behavior of OEG-CCMV and RGDCCMVindicated the inhibition effect of OEG and thepromotion effect of RGD for cell attachment. This providesa generalized method for chemical modification of thesurface of virus capsids with multivalent ligands, whichdemonstrates the potential applications in bioimaging, tissueengineering, and drug delivery.