http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Experimental and Theoretical Studies of Bidirectional Variable Curvature Friction Pendulum Bearing
Shu-Chao Lin,Jian-Qiang Han,Hong-tie Zhao 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.5
In this study, to satisfy the base isolation demand of large-scale building structures under different earthquake records, a new bidirectional variable curvature friction pendulum bearing (BD-VCFPB) is proposed. The VCFPB is designed in detail based on the basic principle of a friction pendulum system. Moreover, the sliding interface function, gap between the slider and the sliding interface, and polytetrafluoroethylene (PTFE) plate stress are studied. Finally, a dynamic cyclic test is conducted to study the influence of the slider size, contact pressure on the PTFE plate, and loading frequency on the hysteretic behavior of the VCFPB. The results indicate that the hysteretic curves of the horizontal force and the displacement are regular, symmetrical, stable, and full, and clearly reflect the variable curvature convex and concave characteristics and the excellent behavior of energy dissipation of the BD-VCFPB. The sliding friction coefficient varies significantly with the contact pressure on the PTFE plate and the loading frequency; however, it has little relation with the slider size. A hysteretic model is proposed based on the results of a unidirectional test, and it is in good agreement with the hysteretic curves of the horizontal force and the displacement from a bidirectional test.
Han, Bao-Lin,Xu, Xiang-Ying,Zhang, Chun-Zhi,Wu, Jian-Juan,Han, Chun-Feng,Wang, Hui,Wang, Xuan,Wang, Guang-Shun,Yang, Shu-Juan,Xie, Yao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6
Objective: To conduct a meta-analysis to investigate the value of EBV DNA in diagnosis of nasopharyngeal cancer (NPC) in Asian populations, and provide important evidence for screening. Methods: Prospective or respective case-control or cohort studies regarding the detection role of EBV DNA for NPC were included in our study. We conducted a comprehensive literature search in PubMed, EMBASE, and the Chinese Biomedical Database (CBM database between January 1980 and March 2012. Results: A total of 18 studies with 1492 NPC cases and 2641 health controls were included. Almost of the included studies were conducted in China, and only one other conducted in Thailand. The overall results demonstrated that the pooled sensitivity, specificity, positive likelihood (+LR) and negative likelihood (-LR) were 0.73 (0.71-0.75), 0.89 (0.88-0.90), 8.84 (5.65-13.84) and 0.19(0.11-0.32), respectively. The overall EBV DNA detection showed the largest area of 0.932 under the summary receiver operator curve (SROC). The accuracy of detection by plasma for NPC (0.86) was higher than in serum (0.81), with largest areas under the SROC of 0.97 and 0.91, respectively. Conclusion: Our results demonstrated the EBV DNA detection in plasma or serum has high sensitivity and specificity in diagnosis of NPC, especially in Chinese populations with a high risk of cancer.
Yu Jian,Li Wen,Hou Guo-jun,Sun Da-peng,Yang Yuan,Yuan Sheng-xian,Dai Zhi-hui,Yin Hao-zan,Sun Shu-han,Huang Gang,Zhou Wei-ping,Yang Fu 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Hepatitis B protein x (HBx) has been reported to promote tumorigenesis in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), but the mechanism awaits further investigation. In this study, we found that cFAM210A (a circular RNA derived from the third exon of transcript NM_001098801 of the FAM210A gene; CircBase ID: hsa_circ_0003979) can be silenced by HBx. cFAM210A expression was downregulated and negatively correlated with tumorigenesis in patients with HBV-related HCC. Furthermore, cFAM210A reduced the proliferation, stemness, and tumorigenicity of HCC cells. Mechanistically, HBx increased the N6-methyladenosine (m6A) level of cFAM210A by promoting the expression of RBM15 (an m6A methyltransferase), thus inducing the degradation of cFAM210A via the YTHDF2-HRSP12-RNase P/MRP pathway. cFAM210A bound to YBX1 and inhibited its phosphorylation, suppressing its transactivation function toward MET. These findings suggest the important role of circular RNAs in HBx-induced hepatocarcinogenesis and identify cFAM210A a potential target in the prevention and treatment of HBV-related HCC.
( Yi Han Liu ),( Shu Ai Fan ),( Xiao Guang Liu ),( Zhi Meng Zhang ),( Jian Ling Wang ),( Zheng Xiang Wang ),( Fu Ping Lu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7
The stability of Bacillus licheniformis alpha-amylase (BLA) under acid condition was enhanced through direct evolution using the error-prone polymerase chain reaction. One beneficial mutation site, H281I, was obtained in BLA. The specific activity of H281I was 161/352 U/mg, which was 62.6/27.5% higher than that of the wild-type (WT) (99/276 U/mg) at pH 4.5/6.5 and 95°C. The pH optimum for H281I was decreased about 1 unit, whereas no significant changes of optimum temperature and thermostability were observed compared with the wild type (WT). The kcat/Km value of H281I was 1.7-/1.4-fold higher at pH 4.5/6.5, respectively, than that of WT. The structure model analysis indicated that the H281I mutation altered the predicted interaction between the amino acid residues at 281 and 273, thus creating a conducive local environment for substrate binding, as reflected by its decreased Km, and consequently increased the specific activity.
Zi-Shu Chen,Ning-Ning Han,Jian Hong Li,Hu Wan,Guo-Hua Huang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The real-time quantitative reverse transcription PCR (qRT-PCR) is an efficient and pervasive technique for analysis gene expression. Despite the fact that this technique has been extensively used to explore the gene functions, the stability of the reference genes still requires being validated at different experimental conditions. This research aims to confirm that the expression stability of seven candidates reference genes under different concentrations sterile hemolymph containing virus (2, 4, 6, 108-fold) infects the Spodoptera frugiperda cells line (Sf9). The appropriate reference genes as endogenous controls were determined through four computational algorithms (ΔCt, NormFinder, BestKeeper, and geNorm) and an integrated online software RefFinder. According to results of this study, TUB and ACT1 were proved to be the most stable reference genes in E2 fold and E4 fold respectively. PPI was the best reference gene in E6 and E8 fold, 28S and TUB were the most stable pair reference genes through all samples. These results can facilitate basic researches of the molecular mechanism of HvAV- 3h and also prove to be helpful for the standard RT-PCR method.
Adenoviral Vector Mediates High Expression Levels of Human Lactoferrin in the Milk of Rabbits
( Zeng Sheng Han ),( Qing Wang Li ),( Zhi Ying Zhang ),( Yong Sheng Yu ),( Bo Xiao ),( Shu Yun Wu ),( Zhong Liang Jiang ),( Hong Wei Zhao ),( Rui Zhao ),( Jian Li ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.1
The limitations in current technology for generating transgenic animals, such as the time and the expense, hampered its extensive use in recombinant protein production for therapeutic purpose. In this report, we present a simple and less expensive alternative by directly infusing a recombinant adenovirus vector carrying human lactoferrin cDNA into rabbit mammary glands. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. An 80-kDa protein was visualized after viral vector infection. With this method, we obtained a high level of expressed human lactoferrin of up to 2.3 mg/ml in the milk. Taken together, the method is useful for the transient high-level expression recombinant proteins, and the approach established here is probably one of the most economical and efficient ways for large-scale production of recombinant proteins of biopharmaceutical interest.
Strength of Double Skin Steel-Concrete Composite Walls
Ying Qin,Gan-Ping Shu,Shenggang Fan,JinYu Lu,Shi Cao,Jian-Hong Han 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.2
Double skin steel-concrete composite walls have been increasingly used in civil engineering applications. However, the advantages of these walls have not been fully recognized due to the lack of appropriate technical guidelines for capacity design. In this paper, the local buckling strength of steel plate were firstly reviewed in terms of specifications incorporated in several modern codes. A methodology to predict the strength of steel plate with restraint of both concrete and shear studs was proposed based on the explicit solution for local buckling of steel plate in composite shear walls subjected to uniform axial compression and with elastically rotational restraint at loaded and unloaded edges. The results were compared with various previous experimental data and good agreement was observed. Furthermore, the load carrying capacity of composite walls was derived from the superposition of the contribution of steel plate and concrete. The predicted values from the proposed equations, together with the resulted determined from modern codes, were compared with the experimental results. It was found that both the proposed method and JEAG 4618 offer reasonable predictions while AISC 360 and KEPIC-SNG always underestimate the actual values.
Deuterium Clusters Fusion Induced by the Intense Femtosecond Laser Pulse
Hong-Jie, Liu,Zhi-Jian, Zheng,Yu-Qiu, Gu,Bao-Han, Zhang,Yong-Joo, Rhee,Sung-Mo, Nam,Jae-Min, Han,Yong-Woo, Rhee,Kwon-Hae, Yea,Jia-Bin, Chen,Hong-Bin, Wang,Chun-Ye, Jiao,Ying-Ling, He,Tian-Shu, Wen,Xia ALLERTON PRESS INC 2007 CHINESE PHYSICS LETTERS Vol.24 No.2
<P>Neutrons (2.45 MeV) from deuterium cluster fusion induced by the intense femtosecond (30 fs) laser pulse are experimentally demonstrated. The average neutron yield 10<SUP>3</SUP> per shot is obtained. It is found that the yield slightly increases with the increasing laser spot size. No neutron can be observed when the laser intensity I < 4.3×10<SUP>15</SUP> W/cm<SUP>2</SUP>.</P>