한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

木浦(나무벌)의 水生 및 濕生管束植物相과그들의 保護

鄭宇珪,孔榮植,梁運眞 경남대학교 환경문제연구소 1995 환경연구 Vol.17 No.-

1993년 7월 부터 1995년 5월 까지 9회에 걸쳐 慶南 昌寧郡準 梨房面 木浦(나무벌)의 水生 및 濕生植物과 그들의 生活形, 開花期 및 花色을 조사한 결과는 다음과 같았다. 1. 조사된 總 植物은 4目 31科 48屬 71種 14變種 2品種 總 87種類였다. 2. 이들 중 水生植物이 11目 20科 23屬 38種 9變種 1品種 總 48種類 55.2%이고, 挻水植物 22種類 45.8%, 浮??植物 15種類 31 3%, 沈水植物 8種類 9.2%, 浮生植物 3種類 6.3%였다. 3. 濕生植物은 10目 24科 25屬 33種 5變種 1品種 總 39種類 44.8%였다. 4. 木浦 植物相의 開花期는 7~8월에 가장 많이 開花하여 여름개화기 후형이었고 北半球 溫帶 氣候 開花期이었다. 5. 木浦 樵物相의 花色 多樣性에서 가장 많은 색은 綠色(33.3%)이었고 다음이 흰색(25.4%)이었다. 6. 溜池에는 마름이 優占種이었고, 가시연꽃이 準優占種이었으며 가시연꽃은 單一種으로 된 群落을 형성하고 있었다. 7. 늪지의 水生 및 濕生植物의 分布 즉 마름, 가시연꽃 자라풀, 수염마름 등의 分布가 논의되었으며 이들 濕地植物과 늪의 保護를 위한 課題도 논의되었다. The flora, distribution, life form, flowering period, and flower colors of hydrophytes and hygrophytes from Mokp,o swamp in Changny□ng-gun, Ky□ngsangnam-do, Korea were investigated at nine times from July, 1993 to August, 1995. The flora was composed of 16 orders, 31 families, 48 genera, 71 species, 14 varieties, and 2 forma; total 87 taxa. Among them, the vascular hydrophytes were composed of 11 orders, 20 families, 23 genera, 38 species. 9 varieties, and 1 forma, total 48 taxa, 55.2%. These were composed of 22 taxa emerged plants(45.8%), 15 taxa floating-leaves plants(31.3 %), 8 taxa submerged plants(9.2%), and 3 taxa free-floating plants(6.3%). The hygrophytes were composed of 10 orders, 13 families, 25 genera, 33 species, 5 varieties, and 1 foema: total 39 taxa, 44.8%. The peak of flowering period occurred in August. The most species in this swamp were flowering from July to August. The pattern of flowering period was responded summer flowering climate pattern and northern middle latitute region flowering climate pattern. In the study of flower color diversity of Mokp,o flora, the most abundunt colcr was green taxa(33.3%) and the white was second(26.4%). In Mokp.o swamp dominent species was Trapa japonica and subdominent was Euryale ferox on the whole. The distribution of hydrophytes: Euryale ferox, Trapa japonica, Hydrocharis dubia and Trapella sinensis var antenifera were discussed. Also the work for protection of the swamp and wetland plants in Mokp.o were discussed.

고감도 CaSO_4 : Dy TL 분말의 TL 특성 및 고감도 TL 소자 제작 Dy TL powder and development of high sensitivity CaSO_4 Dy TL pellet

양정선,박재우,김두영,김장렬,장시영 제주대학교 방사능이용연구소 2002 연구보고 Vol.16 No.-

CaSO_4 : Dy series TL material is widely used as a personal dosimeter because of its high sensitivity to radiation. KAERI(Korea Atomic Energy Research Institute) has been produced CaSO_4 TL powder activated by Dy(rare earth elements) for several years and continually tried to improve its sensitivity having more than that of commercialized Teledyne CaSO_4 : Dy TL powders(USA). The TL powder is prepared by adding 0.1mol% Dy as an activator and by the acid evaporation method at first. And new step of washing this powder by distil-water is appended to separate non-crystalized powder in the initial crystal of CaSO_4 : Dy powder. CaSO_4 : Dy crystal TL powder which is produced by applying this new method has higer sensitivity than before washing step, and it has also higher sensitivity than that of Teledyne CaSO_4 : Dy TL powder. This CaSO_4 : Dy TL powder shows good TL characteristics such as dose response, energy response and fading. It is not possible to make solid detectors of only CaSO_4 : Dy TL phosphor without any binding material. Until now widely used sintered pellets. which were made from a mixture of CaSO_4 : Dy TL phosphor and Teflon. But, due to reduction in content of CaSO_4 : Dy TL phosphor in pellets(15∼20wt%) the TL sensitivity of pellets was reduced correspondingly. To variously use the CaSO_4 : Dy TL phosphor, research for preparing detectors by mixing CaSO_4 : Dy TL phosphor with different binding substances has been conductedThis paper described development of a new type of CaSO_4 : Dy pellets by using P-compounds as a bonding material(KCT-300), and compared the TL sensitivity with that of the commercialized Teledyne CaSO_4 : Dy pellets. Sensitivity of a new developed KCT-300 shows about 6 times than Teledyne ones, and can be used to measure very low radiation dose.

인슐린 주사제의 고충실 액체색층기 분석

우정택,팽정렬,김성운,양인명,김진우,김영설,김광원,최영길 최신의학사 1993 最新醫學 Vol.36 No.8

항공사 승무원의 비행 스트레스와 식행동에 관한 연구

양정미,노정옥,우경자 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.2

This study investigated the relationship among the flight stress, disease and eating behavior of the Airline cabin crews. Self administered questionnaires were collected from three hundred and twenty cabin crews. Statistical data analysis was completed using a SPSS v.10.0 program. The results were summarized as follows: Before the flight, the maladaptation to the oversea foods was the most common stress for the crews less than one year flight experience. During the flight, fastidious arrangement and loss of appetite due to overwork were the most common stresses. After the flight, anorexia due to jet lag was the most common stress. Backache and stomachache were the most common job-related ailments of the airline cabin crews. During the flight, the intakes of carbonated drinks and coffee were most common. After the flight, water was the most common drink they consumed.

용융 알미늄합금의 산화피막 강도측정에 관한 연구

우정명,양중식,백영남 慶熙大學校 材料科學技術硏究所 1991 材料科學技術硏究論集 Vol.4 No.-

This study carrys out to determine the strength of oxide skins of Al alloy melts as the practical alloy for automobile piston. This has been observed by Rotational Die Movement Equiment for the temperature range from 600℃ to 750℃ and oxidation time range from 10min to 60min. This method was able to quantify the strength of oxide skins of the molten alloys and had ±3% experimence error range. This strength of oxide skins of the molten alloys were much more affected by oxidation temperature than oxidation time.

수도권 지역의 의료기기 관련 업체의 실태 조사 연구

양태홍,이우철,김정래,박승환 서울보건대학 1997 서울보건대학 부설 병원경영연구소 논문집 Vol.3 No.1

Recently, the domestic business of medical equipment have many difficult things in the development and sale of new product. To overcome them, it is needed to solidify the co-work system of industry-education marriage. This paper is undertakened to serve an aid in the field of medical equipment. The analysis of data is attempted through the survey by questionaires, and an object of study is the medical business corporation at Seoul area. As a result to review the data it was identifided that this study is able to be applied to the basis material to reconstruct a right role and phase to medical business Also, it`s expected that we're able to use its results as the educational materials to bring up the more qualified students fit to the industrial need.

顔貌變形症 患者에 있어서 顎矯正 手術前 心理 評價에 關한 臨床的 硏究

양명철,신효근,진우정 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.1

The need to assess preoperative psychological characteristics of orthoganathic surgery patients has recently been recognized. Fifty six patients were involved in this study, they were investigated in the preoperative psychosocial aspects. Korean Version Eysenk Personality Questionaire and Korean Version Tennessee Self-Concept Scale were used for psychological test. The results were as follows : 1. There was no significant difference of sex and age in the personality diamension, the selfconcept scale, and the preoperative tests(P>0.05). 2. 37% of subjects showed phonetic problem, 60% masticatory problem and 56% social problem for facial deformity. The first choice was the face in the order of correctionfor mastication, phonation and face. And 83% of the subjects forecasted the change in their face after surgical correction, The answer was generally vague. 64% of the subjects expected their personality to become more lively as a result of their operation. 3. Total positive self score and personal self score were less improved in the groups with high scores of toughmindness and neuroticism(P<0.05), degree of suffering was increased in the group with high scores of neuroticism(P<0.05). 4. Degree of subjective deformity, self-consciousness, and degree of dissimilarity were more improved in the group of intraversion, there was a significant correlation with degree of dissimilarity in the group with high score of toughmindness(r=-.64, P<0.05). 5. There was a significant correlation with the forecast of change in the groups with high score in extraversion(r=.59, P<0.01) and with low score in neuroticism(r=-.39, P<0.05).

방사선 조사를 받은 흰쥐 망막 속얼기층의 미세구조

양석우,고정식,양남길,안의태,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

본 실험은 머리부분이 많은양의 방사선에 노출되었을 때 망막 속얼기층의 미세구조적 변화를 관찰하기 위하여 시행하였다. 실험동물로는 체중 200-250g의 흰쥐를 사용하였으며, 마취상태에서 머리부분에만 방사선을 조사하였다. 방사선 조사는 미쓰비시 선형가속기를 이용하여 조사거리 80cm, 구역 30cm X 30cm, 깊이 1.2cm 조건에서 1분당 200rad씩 연속조사하여, 3,000rad 또는 6,000rad를 조사한 후 6시간, 2일, 6일후에 희생시켰다. 각 동물은 1% glutaraldehyde- 1% paraformaldehyde액으로 관류고정하였다. 관류가 끝난 후 안구를 적출하여 톱니둘레를 따라 절단한 다음, 망막의 일정부위에서 조직을 떼어냈다. 떼어낸 조직은 2.5% glutaraldehyde- 1.5% paraformaldehyde액에 일차고정한 후 1%osmium tetroxide액에 이차고정하였다. 고정된 조직은 수세와 탈수과정을 거쳐 araldite혼합액에 포매한 다음 얇은 절편을 작성하여 uranly acetate와 lead citrate로 염색한 후 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 무축삭신경원의 세포질돌기는 전자밀도가 높은 어두운세포와 전자밀도가 낮은 밝은세포로 구분되었으며, 밝은 세포 가운데에는 과립형연접소포를 갖고 있는 돌기가 관찰되었다. 2. 3,000rad조사 후 6시간군의 경우 밝은 무축삭신경원이 세포질돌기와 신경절세포의 가지돌기는 팽대되었으며 사립체와 세포막이 파괴되는 모습이 관찰되었는데, 2일군의 경우 그 경향이 가장 심했다. 3. 3,000rad조사 후 6일군의 경우 부챗살아교세포의 영역이 넓어져서 전체적인 조직의 모습이 2일군의 것에 비하여 많이 회복되어 보였다. 4. 6,000rad조사 6일군의 경우 부챗살아교세포는 미세사가 많고 전자밀도가 높은 변성된 세포가 자주 관찰되었다. 이상의 결과를 종합해 보면 3,000rad의 방사선을 조사받으면 흰쥐망막의 속얼기층은 처음에는 무축삭신경원의 세포질돌기와 신경절세포의 가지돌기가 손상을 받았으나, 6일 후에는 부챗살아교세포의 영역이 넓어져서 많이 회복되었다. 그러나 6,000rad정도의 방사선을 조사하면 부챗살아교세포도 변성되어 속얼기층 전체가 변성된것으로 보아, 부챗살아교세포는 무축삭신경원이나 신경절세포에 비하여 방사선에 대한 저항성이 높다고 생각된다. This experiment was performed to study the morphological respones of the inner plexiform layer of the retina of rat following X-ray irradiation. Rats anethetized with sodium thiopental, were exposed only on their head areas with a single dose of 3,000rads or 6,000rads, respectively. Radiation was produced by Mitsubishi linear accelerator, at the speed of 200rads/min. The experimental groups were sacrificed on the 6th hour, 2nd hour, 2nd and 6th day after X-ray irradiation. Under anethesia, 1% glutaraldehyde- 1% paprformaldehyde solution was perfused through the heart. Pieces of the tissue taken from the posterior region of the retina were fixed in 2.5% glutaraldehyde- 1.5% paraformaldehyde, and in 1% osmium tetroxide, and embedded in araldite mixture. Ultrathin sections were stained with uranyl acetate and lead citrate solutions. From the ultrastructural study, following results were made: 1. Two types of amacrine cells, according to their elecrton densities, were found, i.e., light amacrine cell and dark amacrine cell. Granular synaptic vesicels were often observed in the light amacrine cells. 2. Two days after exposure to 3,000rads of X-ray, severe morphological changes were observed in cytoplasmic processes of light amacrine cells and dendrite of ganglion cells; i.e., edematous changes, framoentation of mitochondria, breakdown of cell membranes, etc. 3. Six days after exposure to 3,000rads of X-ray, many of the malformaitons were seen to be repaired, except the territory of activated Muller cell. 4. Six days after exposure to 6,000rads of X-ray, darkly degenerated Muller cell were frequently observed. From the above results, it was concluded that large amount of head-irradiation results the severe damages to the cytoplasmic processes of amacrince cell and the dendrites of ganglion cell in the inner plexiform layer of rat retina, but Muller cells are more radioresistant than amacrine and ganglion cells.