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Scrub Typhus : 확진된 76예의 임상 소견에 대한 전향적 연구
김동민,김현리,박치영,윤성호,송현제,심수경 대한감염학회 2006 감염과 화학요법 Vol.38 No.4
배경 : Orientia tsutsugamushi에 의한 인체 감염은 혈액과 임파선을 타고 전신에 퍼져 혈관 염을 일으켜, 전신 장기의 침범 소견을 보인다. 그러나 아직 까지 전신장기 침범에 대한 임상 소견 및 검사 결과의 전향적인 추적 관찰에 대한 연구는 거의 없는 실정이다. 재료 및 방법 : 조선대학교병원에 내원한 환자 중 확진법(gold standard)인 간접형광항체법(IFA)으로. 확인된 76명의 환자를 대상으로 내원시 임상 양상 및 검사 결과의 평가 및 치료 후 검사 결과의 변화에 대한 전향적인 연구를 수행하였다. 결과 : 대부분의 환자에서 발열, 갈증, 전신 쇠약감, 두통을 호소하였으며, 특이 할만한 소견으로 현재까지 보고되지 않았지만, 대부분의 환자가 갈증을 심하게 호소한다는 것이다. 3명의 젊은 건강한 환자를 제외한 대부분의 환자에서 이러한 갈증 증상을 호소하였으며, 이러한 갈증은 발열이 호전되어도 지속되었으며, 퇴원 이후에 까지 지속되는 양상을 보였다. 혈액검사상 scrub typhus 초기에 나타날 수 있는 신기능 장애 및 간 기능 장애는 대부분 가역적으로 적절한 항생제 투여 후 모두 정상으로 회복됨을 확인할 수 있었다. 혈액 검사상 CRP, LDH, AST가 대부분의 환자에서 상승하였고, 특히 LDH의 상승이100% 환자에서 관찰된 것은 특이할 만 한데 적절한 치료후 CRP는 신속히 호전을 보이나, LDH는 서서히 호전됨을 확인하였다. DIC 검사상 DIC는 입원환자의 95.5%에서 확인되었으나, 비교적 일부의 환자에서 출혈 및 경색이 초래되는 것으로 생각된다. 결론 : Scrub typhus 초기에 나타 날수 있는 신기능 장애, 간기능 장애 및 DIC는 대부분 가역적으로 적절한 항생제 투여 후 모두 정상으로 회복되며, 이러한 관찰이scrub typhus 환자의 진단 및 임상 경과의 이해에 도움이 되리라 사료된다. Background : Orientia tsutsugamushi spreads to the entire body through the blood and lymphatics, and it induces vasculitis that results in the patients manifesting symptoms of systemic organ involvement. Materials and Methods : We conducted a prospective study to evaluate the clinical manifestations and the change of the laboratory results after instituting treatment for scrub typhus. Results : Most patients presented with fever (100%), malaise (96.1%) and thirst (96.1%). It was remarkable that most patients presented with severe thirst, except for 3 healthy, young patients. This thirst was persistent even after the resolution of fever. The renal and hepatic dysfunction were reversible after the administration of appropriate antibiotics. For the blood testing, it was deteded that the CRP, and LDH were elevated in most patients (95.9% and 100% respectively). DIC could be diagnosed in 95.5% of the patients at the time of admission Conclusion : Most patients presented with fever, malaise and severe thirst, and the renal and hepatic dysfunction were reversible after the administration of appropriate antibiotics. DIC was observed in most of our patients, but hemorrhage and infarction were not present. CRP showed a rapid improvement, nonetheless, the LDH and DIC test results improved slowly.
다발성 골수종에서 저용량 thalidomide, cyclophosphamide, dexamethasone (TCD) 요법의 효과
류충헌,정재현,고정해,장제혁,박영진,최규남,박봉수,이상민,주영돈 인제대학교 2008 仁濟醫學 Vol.29 No.-
Background and Objectives : The immunomodulatory drug thalidomide can inhibit angiogenesis and induce apoptosis in experimental models. It can also induce marked and durable response in newly diagnosed myeloma patients. Thalidomide has been used at doses ranging from 200 to 800 mg with significant toxicity. No data are available on the impact of low-dose thalidomide, cyclophosphamide and dexamethasone as initial therapy for myeloma patients. Design and Methods : To address this issue, newly diagnosed myeloma patients were treated with 50 mg/day thalidomide continuously and cyclophosphamide 150 mg/m², days 1-4 and dexamethasone 20 mg/m², days 1-5 and day 15-19, every month. Between October 2005 and October 2006, 14 patients (median age 54.5 years) were treated with low-dose thalidomide, cyclophosphamide and dexamethasone. Results : After a minimum of two cycles of treatment, 5 patients (55.5%) showed a partial remission. After four cycles of treatment, 10 patients (83.3%) showed a partial remission (n=6) and complete remission (n=4). After a median follow-up of 15.4 months, 1 year overall survival rate was 82.0%. Thalidomide was well tolerated without serious toxic effects. Conclusions : The combination of low-dose thalidomide, cyclophosphamide and dexamethasone demonstrates favorable response rate and 1 year overall survival rate in newly diagnosed myeloma. Severe toxicities were not seen with this combination.
Lee, Ja-Rang,Kim, Young-Hyun,Park, Sang-Je,Choe, Se-Hee,Cho, Hyeon-Mu,Lee, Sang-Rae,Kim, Sun-Uk,Kim, Ji-Su,Sim, Bo-Woong,Song, Bong-Seok,Jeong, Kang-Jin,Lee, Youngjeon,Jin, Yeung Bae,Kang, Philyong,Hu Hindawi Publishing Corporation 2016 International journal of genomics Vol.2016 No.-
<P><I>TSEN54</I> encodes a subunit of the tRNA-splicing endonuclease complex, which catalyzes the identification and cleavage of introns from precursor tRNAs. Previously, we identified an<I> AluSx</I>-derived alternative transcript in<I> TSEN54</I> of cynomolgus monkey. Reverse transcription-polymerase chain reaction (RT-PCR) amplification and<I> TSEN54</I> sequence analysis of primate and human samples identified five novel alternative transcripts, including the<I> AluSx</I> exonized transcript. Additionally, we performed comparative expression analysis via RT-qPCR in various cynomolgus, rhesus monkey, and human tissues. RT-qPCR amplification revealed differential expression patterns. Furthermore, genomic PCR amplification and sequencing of primate and human DNA samples revealed that<I> AluSx</I> elements were integrated in human and all of the primate samples tested. Intriguingly, in langur genomic DNA, an additional<I> AluY</I> element was inserted into<I> AluSx</I> of intron eight of<I> TSEN54</I>. The new<I> AluY</I> element showed polymorphic insertion. Using standardized nomenclature for<I> Alu</I> repeats, the polymorphic<I> AluY</I> of the langur<I> TSEN54</I> was designated as being of the<I> AluYl17</I> subfamily. Our results suggest that integration of the<I> AluSx</I> element in<I> TSEN54</I> contributed to diversity in transcripts and induced lineage- or species-specific evolutionary events such as alternative splicing and polymorphic insertion during primate evolution.</P>
Lee, Jinwoo,Na, Hyon Bin,Kim, Byoung Chan,Lee, Jin Hyung,Lee, Byoungsoo,Kwak, Ja Hun,Hwang, Yosun,Park, Je-Geun,Gu, Man Bock,Kim, Jaeyun,Joo, Jin,Shin, Chae-Ho,Grate, Jay W.,Hyeon, Taeghwan,Kim, Jungb Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.42
<P>A magnetically-separable and highly-stable enzyme system was developed by adsorption of enzymes in superparamagnetic hierarchically ordered mesocellular mesoporous silica (M-HMMS) and subsequent enzyme crosslinking. Superparamagnetic nanoparticles were homogeneously incorporated into hierarchically-ordered mesocellular mesoporous silica (HMMS) by the decomposition of a preformed iron propionate complex. The size of the incorporated superparamagnetic nanoparticles was around 5 nm, generating a magnetically separable host with high pore volumes and large pores (M-HMMS). α-chymotrypsin (CT) was adsorbed into M-HMMS with high loading (∼30 wt%) in less than 30 minutes. Glutaraldehyde (GA) treatment of adsorbed CT resulted in nanometer scale crosslinked enzyme aggregates in M-HMMS (CLEA-M). The activity of these CT aggregates in M-HMMS (CLEA-M-CT) was 34 times than that of simply adsorbed CT in M-HMMS, due to an effective prevention of enzyme leaching during washing <I>via</I> a ship-in-a-bottle approach. CLEA-M-CT maintained the initial activity not only under shaking (250 rpm) for 30 days, but also under recycled uses of 35 times. The same approach was employed for the synthesis of CLEA-M of lipase (CLEA-M-LP), and proven to be effective in improving the loading, activity, and stability of enzyme when compared to those of adsorbed LP in M-HMMS.</P> <P>Graphic Abstract</P><P>Crosslinked enzyme aggregates in magnetitie-coated mesoporous silica, fabricated <I>via</I> a two-step procedure of enzyme adsorption and crosslinking, were highly active, highly stable, and easily recyclable using a magnet. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b909109b'> </P>
Lee Bonggi,An Hye Jin,Kim Dae Hyun,Lee Min-Kyeong,Jeong Hyeon Hak,Chung Ki Wung,고영훈,Seo Arnold Y.,Kim Il Yong,Seong Je Kyung,Yu Byung Pal,LEE, JAE-WON,Im Eunok,Lee In-Kyu,Lee Myung-Shik,Yamada Ken-ich 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
The vitamin-C-synthesizing enzyme senescent marker protein 30 (SMP30) is a cold resistance gene in Drosophila, and vitamin C concentration increases in brown adipose tissue post-cold exposure. However, the roles of SMP30 in thermogenesis are unknown. Here, we tested the molecular mechanism of thermogenesis using wild-type (WT) and vitamin C-deficient SMP30-knockout (KO) mice. SMP30-KO mice gained more weight than WT mice without a change in food intake in response to short-term high-fat diet feeding. Indirect calorimetry and cold-challenge experiments indicated that energy expenditure is lower in SMP30-KO mice, which is associated with decreased thermogenesis in adipose tissues. Therefore, SMP30-KO mice do not lose weight during cold exposure, whereas WT mice lose weight markedly. Mechanistically, the levels of serum FGF21 were notably lower in SMP30-KO mice, and vitamin C supplementation in SMP30-KO mice recovered FGF21 expression and thermogenesis, with a marked reduction in body weight during cold exposure. Further experiments revealed that vitamin C activates PPARα to upregulate FGF21. Our findings demonstrate that SMP30-mediated synthesis of vitamin C activates the PPARα/FGF21 axis, contributing to the maintenance of thermogenesis in mice.