Enhanced Maturation and Function of Dendritic Cells Using Hydrogel Coated Plate and Antigen Electroporation

Son, Cheol-Hun,Bae, Jae-Ho,Shin, Dong Yeok,Lee, Hong-Rae,Choi, Yung Hyun,Yang, Kwangmo,Park, You-Soo Informa Healthcare 2013 Immunological investigations Vol.42 No.4

<P>Dendritic cells (DCs) are potent antigen-presenting cells that can be matured <I>in vitro</I> from immature dendritic cells (iDCs) in the presence of several biological agents such as cytokine cocktail, CD40L, TNF-a and antigen loading, which are necessary and achieved using various protocols, such as lipofection, passive pulse or electroporation. However, these DCs maturation protocols may cause with a significant loss of cells because of cellular attachment and spreading during culturing. Some biomaterials that influence adhesion and development of cells have been used in cell culture techniques, and it was thought that they might be applied on the culture of DCs. In this study, we used polyHEMA, which is a hydrogel coating biomaterial that prevents DCs from adherence, and investigated whether hydrogel coating affects the maturation of iDCs. The efficiency in the generation of mDCs was improved through hydrogel coating procedure and a dendritic cell maturation marker, CD83, was significantly increased in hydrogel-coated culture condition. The antigen-loaded mDCs from electroporation were further expressed the CD83. The mDCs generated in the hydrogel-coated culture condition showed more, longer and thicker dendrites, and produced more amounts of cytokines such as IL-12 and IFN-γ. Therefore, it was suggested that the hydrogel-coated culture condition could improve function of mDCs.<fn><P>Cheol-Hun Son and Jae-Ho Bae contributed equally to this work.</P></fn></P>

마이크로 컴퓨터용 DBMS를 이용한 데이터 베이스 설계 : 응용시스템 설계를 중심으로

崔鍾烈,朱載勳 釜山大學校 師範大學 1989 교사교육연구 Vol.19 No.-

The efficient management of data is a major activity in individual person or organizations. A significant part of time is spent recording, retriving, and absorbing a large amount of information. Database systems are designed for the convenient and efficient management of it. Databae is referred to a collection of data, and a database management system provides an environment that is to use in retrieving information from and storing it into the database. Today, microcomputer is widely distributed and the DBMS on micomputer is also easily purchased. In this situation, end-user of a computer can design, a necessary database for the efficient management of information without any expert's aids. Therefore he serves as database administrator. Several excellent database texts and reference books are available today, however, most of these books emphasis issues about the design of database management system software and operating system file access methods. On the other hand, many handbooks of a particular DBMS software only demonstrate how it's command can be used for database management. The purpose of this study is to provide adequate technical details for a design of database system with particular microcomputer DBMS software together with practical application while providing the theoretical background necessary to sucessfully implement a wide database systems. So this study consists of two parts, one for concepts of database and general procedures in database design, another for database design of the Graduate School of Management, P university We designed a database system for academic affairs with dBASE Ⅲ plus on microcomputers, IBM AT and XT.

UV 센서를 위한 Spiroxazine LB 단분자막 특성에 대한 연구

최성욱,박은진,장지훈,김재호,강신원 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

경사각에 따른 자리 진동 히트파이프의 열전달특성에 관한 연구

최재혁,윤두호,윤석훈 한국해양대학교 산업기술연구소 2002 연구논문집 Vol.19 No.-

In this paper, heat transfer characteristics of a self-oscillating heat pipe were experimentally investigated for the effect of inclination angle and heat loads. This type of heat pipe consists of a heating section, a cooling section and an adiabatic section. The heat pipe used has a 0.002m internal diameter, a 0.34m length in one turn and consists of 19 turns. Each of the heating and cooling section has a length of 70mm. Experiments were performed to measure the temperature distributions and the pressure variation of the heat pipe according to inclination angle. Based on the experimental results, the heat transfer ability of the self-oscillating heat pipe seems to improved with the increase of the inclination angle.

지역금융시장에서 지역농협의 역할에 대한 실증적 연구 : 부산경남지역을 중심으로 an Empirical Study

최진배,김태훈,민재현 한국경제지리학회 2004 한국경제지리학회지 Vol.7 No.3

지역농협은 협동조합적 금융기관이다. 그러나 지역경제의 금융제약을 완화하기에 적합한 조직구조와 특성을 지니고 있다. 특정지역에서 한정된 고객=조합원을 상대로 영업하고 있기 때문이다. 지금 그것은 심각한 정체성의 위기에 직면하고 있다. 그러나 변화하는 환경에 적응하려고 노력해오면서 지역금융기관으로서 위상을 상당히 구축한 것으로 보인다. 이 글은 지역농협이 지역의 영세기업과 서민가게를 주된 고객으로 하면서 이들의 금융제약을 완화해주는데 많은 기여를 하고 있으며, 나아가 은행권이 상대적으로 관심을 적게 기울이는 농촌지역금융시장에서 독점적인 지위를 누리고 있다는 점을 밝히고 있다. 이 글은 지역농협의 영업기반을 실증적으로 분석한 최초의 글이라 할 수 있다. 한편 이러한 연구결과는 지역농협을 지역금융기관으로 육성해야 할 필요성이 있다는 것을 보여준다. The national agricultural cooperative federation is a kind of credit unions. But because it operates for a small group of members in a specified areas we can say that it has suitable structures and characteristics appropriate to ease regional financial constraints. Now it is suffering identity crisis. But trying to adapt itself to new environments it is changing into a regional financial institute. This paper tries to show that national agricultural cooperative federation serves to ease the financial constraints of small firms and households and has monopoly powers in rural financial markets. This is the first paper that surveys the operational grounds of national agricultural cooperative federation. The results reveal that national agricultural cooperative federation needs to be developed as a regional financial institute.

LPC열의 상관 차원에 의한 화자인식

최성재,이헌규 경원전문대학 2000 論文集 Vol.22 No.-

The thesis is about the methods of speaker recognition by applying strange attractor of linear prediction coefficient series to speaker recognition. We have accomplished speaker recognition experiments by utilizing strange attractor of time series as well as of linear prediction coefficient series. The results were 94% recognition ratios. Therefore, the strange attractor of linear prediction coefficient series was more appropriate characterized parameter in increasing speaker recognition ratios.

중추에서 Prostaglandin계가 Renin-angiotensin System에 미치는 영향

최영태,김종승,문성호,오형균,김재훈,전제열,염철호,윤평진 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.1

Role of prostaglandins on the renin-angiotensin system in the central nervous system was examined in normotensive and 2-kidney, 1 clip (2K1C) hypertensive rats. The experiment was done under thiopental (50 ㎎/㎏, IP) anesthesia. Captopril and indomethacin were injected into the right lateral cerebral ventricle. Arterial blood pressure and heart rate were recorded from the femoral artery. Intracerebroventricular (ICV) captopril (1 ㎎) caused a decrease in mean arterial pressure in both normotensive and 2K1C hypertensive rats. The depressor response to captopril was more sensitive in hyper-tensive rats than in normotensive rats. Indomethacin treatment (ICV, 200 ㎎) altered the depressor response to captopril neither in normotensive nor in hypertensive rats. These results suggest that the cardiovascular effect of renin-angiotensin system in the central nervous system may not be mediated via prostaglandin systems in normotensive and 2KlC hypertensive rats.

(Sr.Ca)TiO_(3)세라믹스의 C-V특성과 Ca의 역할

최운식,강재훈 대불대학교 2002 大佛大學校大學院 硏究論文集 Vol.- No.1

It this study, the capacitance-voltage properties of (Sr_(1-x)·Ca_(x))TiO_(3)(0.05≤x≤0.20)-based grain boundary layer ceramics were investigated. The ceramics were fabricated by the conventional oxide-mixed method. The sintering temperature and time were 1480∼1500℃ and 4hour, respectively. The 2nd phase formed by the thermal diffusion of CuO from the surface leads to very excellent dielectric properties, that is, ε_(γ)>50000, tanδ<0.05, ΔC<±10%. The capacitance is almost unchanged below about 20[V] but it decreases slowly over 20[V]. The results of the capacitance-voltage properties indicated that the grain boundary was composed of the continuous insulating layers. The voltage-current characteristics was due to space charge condensed to interface between grain and grain boundary. The specimens treated thermal diffusion in 1150℃ for 2hrs appeared nonlinearity characteristic of α>7.

소양호의 어류군집 동태

최재석,이광열,장영수,고명훈,권오길,김범철 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.2

2001년 4월부터 2002년 9월까지 소양호의 어류군집 동태를 조사한 결과는 다음과 같다. 조사된 어류는 총 11과 38종이었다. 한국 고유종은 P. tenuicorpus, C. splendidus, S. gracilis majimae, S. japonicus coreanus, H. mylodon, G. brevibarba, M. yaluensis, M. longidorsalis, C. koreensis, C. rotundieaudata, S. microdorsalis, P. koreanus, L. andersoni, C. herzi, O. patycephala 등 15종(40.54%)이었다. 우점종은 Z. platypus(39.60%), H. olidus(39.47%), S. gracilis majimae(12.24%), S. japonics coreanus(2.11%), P. fulvidraco(1.48%), L. macrochirus(1.40%) 등이었다. 채집된 어종들의 생체량을 보면 Z. platypus가 19.48㎏, H. olidus로 8.75㎏, C.cuvieri 7.42㎏, L. maeroehirus 7.39㎏, H. labeo 6.85㎏의 순으로 나타났다. 소양호에서 확인된 도입종은 A. japonica, H. eigenmanni, H. olidus, P. altivelis, C. urotaenius, R. giurinus, T. brevispinis, C. carpio(Israeli type), c. euvieri, Onchorhynchus mykiss, L. macrochirus, M. salmoides, I. puntatus 등 13종이었다. 이 중 C. carpio(Israeli type), C. cuvieri, Onchorhynchus mykiss, L macrochirus, M. salmoides, I. puntatus는 국외종이며, A. japonica, H. eigenmanni, H. olidus, P. altivelis, C. urotaenius, R. giurinus, T. brevispinis 등은 국내 다른 하천ㅇ로부터 유입된 종이다. The dynamics of the fish community in Lake Soyang, Korea, was investigated from April 2001 to September 2002. During the surveyed period 38 species belonging 11 families were collected. There were 15 Korean endemic species 39.47%, including Pseudopungtungia tenuicorpus, Coreoleuciscus splendidus, Squalidus gracilis majimae, S. japonicus coreanus, Hemibarbus mylodon, Gobiobotia brevibarba, Microphysogobio yaluensis, Microphysogobio longidorsalis, Iksookimia koreensis, Iksookimia rotundicaudata, Silurus microdorsalis, Pseudobagrus koreanus, Liobagrus andersoni, Coreoperca herzi, and Odontobutis platycephala. Dominant species were Zacco platypus (39.60%), Hypomesus olidus (31.70%), Squalidus gracilis majimae (12.24%), Squalidus japonicus coreanus (2.11%), Pseudobagrus fulvidraco (1.48%) and Lepomis macrochirus (1.40%). The biomass of collected fishes were Zacco platypus (19.48 ㎏), Hypomes olidus (8.75 ㎏), Carassilus cuvieri (7.42 ㎏), Lepomis macrochirus (7.39 ㎏) and Hemibarbus labeo (6.85 ㎏). Of the 13 introduced fishes in Lake Soyang Cyprinus carpio (israeli type), Carassius cuvieri, Onchorhynchus mykiss, Lepomis macrochirus, Micropterus salmoides and Ictalurus puntatus were originated from foreign countries but Anguilla japonica, Hemiculter eigenmanni, Hypomesus olidus, Plecoglossus altivelis, Chaenogobius urotaenius, Rhinogobius giurinus. Thridentiger brevispinis were introduced from other native river systems.

PCR과 SDS-PAGE를 이용한 젖산균의 분류

최동훈,이중림,민승기,박종환,김해영,조재선 慶熙大學校 1998 論文集 Vol.27 No.-

Strain type of lactic acid bacteria was analysed by PCR and SDS-PAGE. PCR allows the rapid and specific detection of lactic acid bacteria using primers based on 16S rRNA gene-sequences. The analysis of protein pattern on SDS-PAGE can be used to determine the differences among the same or similar species of lactic acid bacteria. Therefore, the complementary use of PCR and SDS-PAGE method is one of simple way to classify the lactic acid bacteria and to compare with genetic analysis.