7두의 개에서 배설성 요로조영술을 통한 신장 기능 평가

최윤정,이기자,최형준,이용진,박성준,송근호,정성목,최호정,이영원 忠南大學校 獸醫科大學 動物醫科學硏究所 2005 動物醫科學硏究誌 Vol.13 No.-

Excretory urography is a type of contrast study used to verify and localize upper urinary tract disease. It is an inexpensive and easy way to visualize of anatomic and functional status of the kidney and has been used as a primary imaging modality for the evaluation of urinary tract abnormalities. We describe urological signs of 7 dogs who examined with excretory urography and ultrasonography. Six cases were referred to veterinary medical teaching hospital. Chungnam national university (VMTH, CNU) to evaluate renal function after the treatment for renal failure and one case was referred showing hematuria. In case 1, 2 and 4, blood test and urinalysis was normal and the results of excretory urography presented that renal function were normal range. In case 3, the results of urinalysis, ultrasonography, and excretory urography except blood test presented abnormal kidney and hydronephrosis was diagnosed. In case 5 and 6, blood test, urinalysis, ultrasonography and excretory urography indicated renal failure. In case 7, blood test, urinalysis and ultrasonography presented partially abnormal signs, however, the results of excretory urography was normal range.

김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

수평 매복된 상악 견치의 교정적 견인

최형준,이종은,이제호,이종갑 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.4

치아 매복은 구강점막이나 악골내에서 치아의 맹출이 중지된 상태를 의미하며, 제 3구대구치와 상악 견치에서 호발한다. 상악 견치의 매복은 주로 측절치가 상실되거나 왜소한 경우와 연관성이 높지만 치배의 위치 이상과 같이 유전적인 요소와도 관련될 수 있다. 매복치는 심미적, 기능적으로 여러 가지 문제점을 야기하므로 조기에 진단하여 치료하는 것이 중요하며, 매복치의 상태에 따라서 치료 방법을 적절하게 선택하여야 한다. 본 증례는 11세 여아에서 구개측으로 수평 매복된 상악 우측 견치의 치료를 보고한 것으로, 매복치는 외과적 수술을 동반한 교정적 견인 방법을 사용하여 기능적, 심미적으로 회복되었다. 견인 치료는 매복 견치의 맹출 공간을 확보하면서 이루어졌으며, 치료 종료 후 매복 견치는 치근 흡수와 같은 합병증 없이 적절한 부착치은을 확보하여 양호하게 위치되었다. Tooth impaction is defined as a cessation of the eruption of a tooth at the level of the oral mucosa or alveolar bone. Maxillary canines are the most frequently impacted teeth next to the third molar. Maxillary canine impaction is associated with congenital missing of lateral incisors, peg lateralis and genetic factors such as ectopic positioning of a tooth germ. The clinicians have an important role in early detectionof tooth impaction for prevention of esthetic and functional problems. There are specific methods to treat impacted tooth for different conditions. In this case, an 11-year-old girl with a horizontally impacted maxillary right canine in a palatal position was treated through orthodontic traction along with surgical button attachment procedure. On regaining of eruption space, canine traction was performed. At the completion of treatment, the canine was positioned fairly within the arch with proper keratinized gingiva and complications such as root resorption were not observed.

Open-face Stainless Steel Crown을 이용한 유전치의 치료에 대한 증례보고

최형준,이제호,최병재,김종민 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.4

In spite of the improvements of the techniques in the field of preventive dentistry, many children still present with extensive destruction of primary anterior teeth. Not only the practioner must consider the pulp state of the primary incisor, but also restore the form, function and esthetics of the tooth. Restorative treatment of primary incisor tooth requires durability, retention and esthetics. Stainless steel crowns used in restoring primary anterior teeth is retentive and durable in comparison with the composite resin, celluloid crown. But they are not esthetic. To enhance the esthetics of the anterior stainless steel crown without reducing its superior retention, an open-face stainless steel crown has been suggested. Several authors have suggested cutting away the labial portion of the stainless steel crown and placing the composite resin in that area. By following this technique, the practioner can prepare a retentive, durable, and esthetic restoration for primary teeth which have suffered from extensive loss of teeth structure. In addition, the single missing primary anterior teeth can be successfully restored by soldering the stainless steel crown together. Open-face stainless steel crown is indicated in the areas of large interproximal lesions involving incisal edge, crown fracture with pulp exposure and congenital malformation of the teeth. By this technique, the practioner can restore primary anterior teeth successfully regardless of the amount of remaining tooth structure, bruxism habit and presence of attrition. In this case, rampant caries with extensive loss of tooth structure and single missing of primary anterior tooth hart been successfully treated with open-face stainless steel crown.

상악 영구 중철지의 외상성 치근파절 : 증례보고 A CASE REPORT

최형준,곽지윤,이종갑,최병재 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.3

청소년기에는 두개악안면부위의 외상성 손상이 흔하다. 그러나 상아질, 백아질, 치수를 포함하는 치아파절로 정의되는 치근파절은 비교적 드물다. 치근파절에 대하여 취할 수 있는 적절한 조치는 파절편의 치관부위를 재위치시키고, 2∼3달간 레진강선 고정술을 시행하는 것이다. 치수과사나 치근흡수의 징후가 나타날때까지 근관치료를 보류해야 하는데 대부분의 증례에서 파절편의 치근부위는 그 생활력을 유지하기 때문이다. 본 증례의 10세 4개월된 남아는 상악영구중절치의 치근파절을 주소로 연세대학교 치과병원에 내원하엿으며, 그후 7년동안 동일부위에 2번의 반복적인 외상이 있었으나 파절편 치관부의 재위치, 레진 강선 고정술, 근관치료 시행 후 임상적으로 방사선학적으로 비교적 양호한 치료결과를 얻었기에 보고하는 바이다. Traumatic injuries in the young permanent dentition are common, but root fractures, defined as fractures in volving dentin, cementum and pulp, are relatively uncommon. Appropriate management of root fracture involves repositioning the coronal portion of the tooth fragment and firm immobilization with a splint for 2 to 3 month. Root canal treatment should not be initiated until the sign of necrosis or resorption are apparent because in most cases, the apical fragments maintain their vitality. The following case report describes a patient with root fractures injured three times over the period of 7 years. The results, clinically and radiographically, were acceptable, but term periodic evaluation is required.

중학교 사회과 경제 관련 교육과정 내용 요소 분석

최병모,박형준,전희옥 한국경제교육학회 2004 경제교육연구 Vol.11 No.2

구순 구개열 환아의 수유 보조 장치에 대한 증례보고

최형준,손흥규,최병재,박동석 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.2

Cleft lip, cleft palate, cleft lip and palate are the most common congenital anomalies in cranio-orofacial region. These anomalies lead to esthetic problem as well as to difficulties in feeding and speech and also to chronic infection of upper airway and to disturbance of jaw growth during growing up. Especially, some difficulties during feeding make parents to be confused and puts infants in dangerous position. But these difficulties can be controlled by proper feeding methods according to pattern of cleft lip and palate. Among these feeding methods, feeding appliance is the most safe and relatively easy to manufacture. Early wearing of this appliance makes cleft lip and palate infants to a normal feeding and weight gain. As a member of team approach for the treatment of cleft lip and palate, a pediatric dentist should solve the feeding problems which cleft lip and palate infants and its parents are confronted and help in receiving further treatment.

수종의 저장용액에서 치주인대세포의 생존율 비교

최원경,최병재,최형준,이종갑 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.2

치아탈구 발생시 사고 현장에서 쉽게 구할 수 있는 저장용액을 이용하여 치주인대세포의 생존율을 비교해보고자 치주인대세포를 10%FBS함유 α-MEM에서 배양한 후 α-MEM에서 저장한 군을 대조군으로 하고 동일한 조건하에서 저온살균된 우유군, 멸균된 생리적 식염수군, 비자극성 타액군, 건조된 상태로 방치한 군을 실험군으로 하여 25℃ 실온에서 세포 배양 30, 60, 90, 120, 180분 후에 각 군에 대해 MTTassay를 시행하여 다음과 같은 결론을 얻었다. 1. 각 조사시점에서 실험군간의 비교에서는 모두 유의성있는 차이를 보였으며 저온살균된 우유군에서 치주인대세포의 생존율이 가장 높았고, 멸균된 생리적 식염수군, 비자극성 타액군, 건조된 상태로 방치한 군 순으로 점차 감소하였다.(P<0.05) 2. 각 실험군내에서 조사시점간의 비교에서는 치주인대세포의 생존율이 시간이 경과함에 따라 유의성있게 감소하였으나(p<0.05). 저온살균된 우유군에서 90-120분 경과 사이. 멸균된 생리적 식염수군에서 60-90분과 120-180분 경과 사이에서는 유의성있는 차이는 없었다.(P>0.05) 이상의 결론을 종합해볼 때 외상으로 인한 치아탈구 발생시 저장용액으로 HBSS가 추천되고 있으나 사고 현장에서 쉽게 구할 수 있고 치주인대세포의 생활력 보존에도 유리한 저온살균된 우유에 탈구된 치아를 담가오도록 추천할 수 있겠다. Preservation of the remaining periodontal ligament cells on an avulsed tooth is very important to the successful outcome of replantation. HBSS is recommended as the most suitable storage medium for the avulsed tooth that cannot be replanted immediately. But their availability near the site of an accident is doubtful. The purpose of this in vitro study was to compare periodontal ligament cells stored in different storage media obtained easily on th spot. Human periodontal ligament cells were collected from the premolar teeth extracted for orthodontic treatment. Cells were cultured in α-MEM culture medium containing 20% FBS, at 37℃ 100% humidity, in a 5% CO₂2 incubator. Cells were cultured in 96 well culture plate, 5×10⁴cells per well with α-MEM and incubated for 24 hours.After discarding the medium, those cells were cultured in α-MEM contained with 10% FBS, pasteurized milk, sterilized saline, unstimulated saliva and bench-dried state at 25℃ room temperature for 30, 60, 90, 120, 180 minutes respectively. And then each group was measured using MTT assay. The results were as follows. 1. Between the group of each time, there was statistically significant difference. Periodontal ligament cells viability was highest in pasteurized milk and was reduced stepwisely in sterilized saline, unstimulated saliva and bench-dried state(p<0.05) 2. between the time of each group, there was statistically significant difference (p<0.05) but was no statistically significant difference at 90 - 120 minutes in pasteurized milk and at 60 - 90 minutes and 120 - 180 minutes in sterilized saline(p>0.05). In conclusion, HBSS as storage medium of an avulsed tooth is not practical on the spot. Insteadily pasteurized milk can be recommended to maintain the periodontal ligament cells viability.

혼합치열기 반대교합자에서 상악골 전방견인장치를 이용한 치료증례

최형준,이제호,최병재,김은영 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

The prevalence of class Ⅲ malocclusion is approximately 5∼9%, and about one fourth of this malocclusion is due to underdeveloped maxilla. Maxillary protraction appliance is an orthopedic device which promote the growth of a deficient maxilla by applying extraoral force to actively growing patients. The object of using maxillary protraction appliance is to guide a normal growth of maxilla and mandible and improve the occlusal relationship and also improve the facial profile. The author treated three patients whom were diagnosed as a class Ⅲ malocclusion due to deficient maxilla using maxillary protraction appliance and the followings are the conclusions : 1. In these cases, anterior crossbite was corrected by anterior movement of maxilla and downward backward rotation of mandible and simultaneously, anterior facial height was increased. 2. The amount of dental change compare to skeletal change was greater as the patients got older. 3. When 500gm of force to each side was applied, the treatment period has been decreased. 4. As a result of applying the force between maxillary first primary molar and canine, there was a small degree of changes in palatal plane. So, it can be concluded that the maxillary protraction appliance is effective in treating growing patients with a deficient maxilla.

Lesch-Nyhan syndrome 환아의 oral self-mutilation 에 대한 증례보고

최병재,최형준,이제호,전진용 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

1. 하순에 심한 self-mutilation wound를 야기한 Lesch-Nyhan Syndrome 환아에서 상하악 유전치에 치관부 절단 및 치수 절세술을 시행하여 치아에 의한 자해를 방지하였다. 2. 장치를 이용한 치료가 어려운 경우 발치가 고려되기도 하나 본 증례에서는 발치보다 보존적인 술식으로 만족할 만한 결과를 얻었다. Self-injurous behavior is defined as deliberate harm to one's own body without suicidal intent. It usually occurs as head banging or hitting, skin cutting, or finger biting and includes ocular, genital and oral self-mutilation. Lesch-Nyhan syndrome is a rate X-linked recessively inherited disorder, caused by complete absence of hypoxanthin-guanine phosphoribosyl transferase(HPRT) activity. Clinical presentation is characterized by mental retardation, chorea, athetosis, hyperuricemia, uricosuria and self-mutilating behavior. In these patients, the most typical feature is loss of tissue from biting themselves, even though they are not insensitive to pain. The dental management of self-mutilation includes treatment with appliances such as soft mouthgrard or lip bumper, extraction of all the teeth, and orthognathic surgery. We report a 25-month-old boy who was a known case of Lesch-Nyhan syndrome and presented with severe self-mutilation wound on his lower lip. Vital pulpectomy and coronal resection was done as a more conservative approach than extracting all primary anterior teeth. Due to maintaining the root portion of the teeth in the bone, it is expected that the normal growth of the alveolar bone will be achieved.