고두께 유리-실리콘의 대면적 양극접합에 관한 연구

김건년,이보나,박효덕,신상모,이근혁,권혁채 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

In this paper, we studied the anodic bonding of 5-inch silicon and #7740 Pyrex glass wafers with a thickness of 3mm by using the EV501 bonder and the Karl Suss SB6 bonder. The test conditions for anodic bonding of the EV501 bonder system with a full field electrode were temperature of 400 ℃ and voltage of 800V in a chamber pressure of 1X10^(3)mbar. The SB6 bonder with a star shaped electrode was tested at the temperature and voltage of 450 ℃ and 1300V in the atmosphere, respectively. As the results of test, we obtained the void free samples regardless of shape of substrates such as etched wafers with cavities and drilled glasses with holes.

Si Bulk Micromachining을 위한 Wafer Rolling Etching 및 그 특성

김건년,이보나,박효덕,신상모,공경준,장동근,김병철,권혁채,이봉희 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

A wafer rolling etching system for the silicon bulk micromachining has been designed and fabricated. The silicon diaphragms were anisotropically etched in a 24.5 weight percent KOH solution. Compared to the conventional KOH etching systems, pyramidal hillocks, and wave-shaped structures on the etched surfaces were greatly reduced by using this system. After etching for time of 438 minutes, the average etched depth and the etch-rate were measured to be 537μm and 1.22μm/min, respectively. The average etching uniformity of etching depth was 0.87% in 5-inch wafer. Our results showed that the wafer rolling method enhanced etch uniformity and etch rate.

집적화된 실리콘 압력센서의 출력전압 보상파라미터 추출 및 그 특성

이보나,김건년,박효덕,신상모,이경탁,김찬,권혁채,이상조,박현주 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

An integrated silicon pressure sensor has been designed, fabricated and tested. The signal conditioning circuits were designed to include calibration and temperature compensation of output voltage through trimming of diffusion and ion-implanted resistors. Before trimming of resistors, the compensation parameters such as pressure sensitivity, temperature coefficient of pressure sensitivity, temperature coefficient of piezoresistors and pressure sensitivity of piezoresistors were measured. Then offset voltage, span, and temperature coefficients of offset voltage and span were calibrated by trimming of resistors. The measured output voltage met our design specification and simulation value above room temperature. But, the measured output voltage at -30°C deviated from our design specification and simulation value because the offset voltages were found to vary randomly as a function of temperature.

보문 : 참당귀 추출물의 나노입자를 통한 피부 미백 효과 연구

권혁년 ( Hyuk Nyun Kwon ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.3

Angelica gigas (AG) root extract is one of the most widely used herbal medicines in Asia and is known to have antioxidant activity and skin whitening effect. This study was carried out to evaluate skin-whitening effects of nanoparticles of AG root extract on cell penetration by comparison with AG root extract, and lecithin and cholesterol were used to encapsulate AG root extract to make nanoparticles. Nanoparticles were spherical liposomes by SEM (scanning electron microscope) and varied in size from a range of approximately 100~500 nm compared to 1,000~5,000 nm for ethanol extracts as measured by ELS (electrophotonetic light scattering spectrophotometer). Cell viability of the nanoparticles in NIH/3T3 fibroblasts was similar to that of the extract. Mushroom tyrosinase inhibition activity of nanoparticles was lower than the extract. Confocal fluorescence micrographs using fluorescent dye showed that the nanoparticles more effectively penetrated into NIH/3T3 fibroblasts than the ethanol extract with higher affinity and mobility to the cells. From these results, it is speculated that nanoparticles could be applied as additive in cosmetics.

보문 : 화장품 소재를 위한 오디 발효 추출물의 항산화 효과

권혁년 ( Hyuk Nyun Kwon ) 대한미용학회(구 대한미용과학회) 2013 대한미용학회지 Vol.9 No.3

The present study aims to evaluate and compare the fermented (FEEM-fermented extract from mulberry produced by EM) and unfermented (WE-water extract from mulberry) extracts from mulberry in activities of antioxidant and antiinflammation as a functional ingredient for cosmetic products. Effective microorganism fermented extract was produced by fermentation of effective microorganisms (EM) containing more than one hundred bacterial strains, including photosynthetic bacteria, lactic acid producing bacteria and yeast. WE has been added to the EM at the concentrations of 0.1~2.5 mg/mL and then they were incubated to make FEEM at 37℃for 7 days. Cell viability of FEEM was measured on RAW 264.7 cells through MTT assay and it showed higher than that of WE in each concentration. Also, the generation of reactive oxygen species by RAW 264.7 cells was measured using flow cytometry caliber (FACS) analysis. FEEM resulted in lesser generation of ROS than WE as revealed by decrease in dichlorofluorescein (DCF) fluorescence. Electron donating abilities (EDA) and nitrite scavenging activities of FEEM were 7~12%, 11~20% respectively and these values were higher than WE at each concentration. In SOD-like activity, FEEM (46.91%) was 11.28% higher than WE (35.63%) at the 2.5 mg/mL concentration and they were lower than L-ascorbic acid at the same concentration. This study showed that FEEM improved the activities of antioxidant and anti-inflammation, and which can be further developed as a functional additive to cosmetics.

플로팅 구조물의 파랑중 모형실험

김혁년(Kim, Hyuk Nyun),채지용(Chae, Ji Yong),박형권(Park, Hyung Kwon),이영욱(Lee, Young Wook) 한국콘크리트학회 2014 한국콘크리트학회 학술대회 논문집 Vol.2014 No.5

^60Cobalt γ-선 조사로 유발된 백서의 유방 종양

신대균,권혁년,황호원,최찬,문형배 圓光大學校 醫科學硏究所 1992 圓光醫科學 Vol.8 No.1-2

This study was aimed at examining the incidence and histological type of the breast tumors of rat induced by ^60Co γ-ray irradiation. Twenty Sprague-Dawley rats were given 0.9 Gy of ^60Co γ-ray for 6 weeks (0.15 Gy per week), and were killed 46 weeks after the end of irradiation. Juvenile fibroadenoma(JFA) was the most frequent tumor(18 of 20), and adenoma(2 of 20) was the second frequent one. The 18 cases of JFAs showed broad spectrum of morphologic changes. The 2 cases revealed massive fibrosis of intralobular stroma(JFA with massive fibrosis), the 6 cases typical JFA, the 6 cases showed focal alveolar hyperplasia(JFA with focal alveolar hyperplasia), and the rest 4 cases showed diffuse alveolar hyperplasia(JFA with diffuse alveolar hyperplasia). Although the rats were not pregnant nor lactating, 2 cases of adenoma were morphologically identical with lactating adenoma. These results suggest that the responsiveness of breast tissue to the radiation is different even in the same species of animals. JFA can be easily made in Sprague-Dawley rats by ^60Co γ-ray irradiation, and this method can be used as a good experimental model in studying the JFA.

Hepatotoxicity of Microcystin-LR in Rats and Mice

Bum-Seok Kim,Hyuk-Nyun Kwon,Chae-Woong Lim,Byung-Moo Rim 한국실험동물학회 2000 한국실험동물학회 학술발표대회 논문집 Vol.2000 No.-

Lipopolysaccharide가 Lymphokine의 합성에 미치는 영향

박영순,권혁년 圓光大學校 基礎自然科學硏究所 1996 基礎科學硏究誌 Vol.14 No.2

Bacterial lipopolysaccharide(LPS)가 림프구의 lymphokine 합성과 분비능에 미치는 영향을 연구하고자 in vitro 및 in vivo로 LPS를 투여한 결과 다음과 같은 결과를 얻었다. 1. In vitro LPS 와 lymphokine합성과 분비능은 영향을 받지 않았으나 in vitro 투여 후에는 IL―2 및 γ―IFN의 분비능은 감소하고 IL―4의 분비능은 증가 하였다. 2. E.coli LPS 와 salmonella LPS의 lymphokine 생성능은 동일하였고 가열한 것과 비가열한 것의 결과도 동일 하였다. 3. LPS 장기 투여로 탈 감작화된 mouse의 lymphokine생성에 대한 효과도 동일 하였으며 in vitro RU486 처치 후에는 lymphokine생성능이 억제 되었다. 이상의 결과를 종합하면 LPS는 IL―2 및 γ―IFN의 합성과 생성능은 감소시킨 반면 IL―4의 생성능은 증가시켰으며, 이들은 glucocorticosteroid 분비에 의해 중재됨을 추측 할 수 있었다. This study was undertaken to evaluate the immunomodulatory properties of the bacterial lipopolysaccgaride(LPS) on the lymphokine production in vivo and in vitro. The results are follows: 1. There was no differences on the lymphokine secretion by the in vitro treatment of LPS, however, in vitro LPS treatment decreased the production of IL―2 and γ―IFN, whereas it increased the IL―4 production. 2. There were no differences on the lymphokine production between E.coli and salmonella LPS, and between heated and non heated LPS. 3. Lymphokine production of mice which were desensitized by the long term treatment was not different from control and in vitro treatment with HU486 can block the alterations of lymphokine production after the treatment of LPS. By the summarizing of these results, LPS decreased the secretion of IL―2 and γ―IFN ana increased the secretion of IL―4 through the endogenous secretion of glucocorticoids.

Hepatotoxicity Induced by Microcystin-LR in Rat

Bumseok Kim,Jae Woo Cho,Hyuk Nyun Kwon,Ivar Blank,Irina Borisova,Sohail Ejaz,Irina Chekarova,Jungkee Kwon,Chae Woong Lim 한국독성학회 2006 Toxicological Research Vol.22 No.4

Microcystin-LR (MC-LR) is a cyanobacterial hepatotoxin mainly produced by Microcystis aeruginosa. The current study examined the effects of a single intraperitoneal dose of MC-LR in rats. Female Sprague-Dawley rats were intraperitoneally injected with MC-LR (100 ㎍/㎏ body weight) and they were sacrificed at 0, 20, 40, 80, 160 min, or 12 h after injection. Clinically, animals showed lethargy and had ruffled hair beginning at 40 min post injection. In the gross findings, liver was enlarged and its color was changed into dark red beginning at 40 min post injection. Microscopically, dissociation of centrilobular hepatocytes and hemorrhage was observed in the hepatic central regions and such pathological changes were then extended to the portal regions of liver by time course manner. Interestingly at 80 min after MC-LR injection, the entrapped eosinophilic materials that may be necrotic fragments of dissociated hepatocytes were found in the capillaries of lung and renal glomerulus. Ultrastructurally, microvilli of the hepatocytes were disrupted or lost at all time points. Furthermore, the Disse space and gap junctions were widened beginning at 40 min post injection. These results suggest that liver is the major target organ of MC-LR and isolated hepatocytes by the effects of such hepatotoxin may secondarily reduce the physiological function of lung and kidney.