Assessing the Diagnostic Value of Serum Dickkopf-related Protein 1 Levels in Cancer Detection: a Case-control Study and Meta-analysis

Jiang, Xiao-Ting,Ma, Ying-Yu,Guo, Kun,Xia, Ying-Jie,Wang, Hui-Ju,Li, Li,He, Xu-Jun,Huang, Dong-Sheng,Tao, Hou-Quan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: This study aimed to summarize the potential diagnostic value of serum DKK1 levels in cancer detection. Materials and Methods: Serum DKK1 was measured using enzyme-linked immunosorbent assay in a case-control study. Then we performed a meta-analysis and the pooled sensitivity, specificity, diagnostic odds ratio, and summary receiver operating characteristic (sROC) curves were used to evaluate the overall test performance. Results: Serum DKK1 levels were found to be significantly upregulated in gastric cancer as compared to controls. ROC curve analysis revealed an AUC of 0.636, indicating the test has the potential to diagnose cancer with poor accuracy. The summary estimates of the pooled sensitivity, specificity and diagnostic odds ratio in meta-analysis were 0.55 with a 95% confidence interval (CI) (0.53-0.57), 0.86 (95%CI, 0.84-0.88) and 12.25 (95%CI, 5.31-28.28), respectively. The area under the sROC was 0.85. Subgroup analysis revealed that the diagnostic accuracy of serum DKK1 in lung cancer (sensitivity: 0.69 with 95%CI, 0.66-0.74; specificity: 0.95 with 95%CI, 0.92-0.97; diagnostic odds ratio: 44.93 with 95%CI, 26.19-77.08) was significantly higher than for any other cancer. Conclusions: Serum DKK1 might be useful as a noninvasive method for confirmation of cancer diagnosis, particularly in the case of lung cancer.

A Study on Major Factors Influencing Dry Cutting Temperature of AISI 304 Stainless Steel

Hui-Bo He,Hua-Ying Li,Jun Yang,Xian-Yin Zhang,Qi-Bin Yue,Xue Jiang,류성기 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.10

TiN coated and uncoated tools were used to conduct the dry cutting temperatures for AISI 304 stainless steel. Effects of cutting variables on cutting temperatures were investigated using single factor experiment method. The optimal cutting parameters of cutting temperatures were obtained through orthogonal experiment method. The forming process and temperature distribution of chips were analyzed using finite element method (FEM). The results show that cutting temperature generally increases with the increase of cutting speed and feed rate. When depth of cut increases at the range of 0.8-1.5 mm, the cutting temperature increases firstly and decreases subsequently. And the cutting temperature of TiN coated tool is lower than that of uncoated tool. The optimal parameters of cutting temperature are obtained by orthogonal experiment, which is A1B1C3. The highest temperature appears on the second deformation zone by the simulation.

Resveratrol Attenuates Early Diabetic Nephropathy by Down-Regulating Glutathione S-Transferases Mu in Diabetic Rats

Bei Jiang,Ling Guo,Bao-Ying Li,Jun-Hui Zhen,Jian Song,Tao Peng,Xiang-Dong Yang,Zhao Hu,Hai-Qing Gao 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.6

Diabetic nephropathy (DN) is the major cause of end-stage renal disease. Resveratrol has been shown to ameliorate hyperglycemia in diabetic rats. However, the effects of resveratrol on DN remain unknown. The aim of the present study is to investigate the effects of resveratrol on early-stage DN. Diabetes was induced by streptozotocin injection in male Wistar rats. The diabetic rats were treated with resveratrol at a dose of 20 mg/kg body weight for 8 weeks. Plasma glucose, creatinine, kidney/body weight ratio, and 24-h urinary protein were determined. The renal pathological changes were examined with periodic acid Schiff staining, and renal mesangial cells were cultured in high glucose concentrations with indicated concentrations of resveratrol (2.5, 5.0, and 10.0 lmol/L). The proliferation of mesangial cells was evaluated by methylthiazoletetrazolium assay. Expressions of glutathione S-transferases Mu (GSTM) and nuclear factor erythroid 2-related factor 2 (Nrf2) were detected by western blot, and apoptosis was analyzed using a flow cytometer. Resveratrol reduced plasma glucose, creatinine, and urinary protein excretion, and attenuated renal hypertrophy. Moreover, resveratrol also reduced the expression of GSTM in diabetic rats. In vitro, resveratrol inhibited the proliferation of mesangial cells caused by high glucose and down-regulated GSTM and Nrf2 expressions in a dose-dependent manner. These findings suggest that resveratrol help prevent the progression of DN. The renoprotection by resveratrol is in part mediated through the inhibition of high glucoseinduced rat mesangial cell proliferation and downregulation of GSTM expression.

Down-regulated serum microRNA-101 is associated with aggressive progression and poor prognosis of cervical cancer

Wei Jiang,Jia-Jia Pan,Ying-Hui Deng,Mei-Rong Liang,Li-Hua Yao 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.6

Objective: MicroRNAs (miRNAs) play a vital role in pathogenesis and progression of many cancers, including cervical cancer. However, importance of serum level of miR-101 in cervical cancer has rarely been studied. In the present study, clinical significance and prognostic value of serum miR-101 for cervical cancer was investigated. Methods: Association between miR-101 level in cervical cancer tissues and prognosis of patients was analyzed by using data retrieved from The Cancer Genome Atlas (TCGA) database, which was followed with our clinical study in which miR-101 serum level comparison between cervical cancer patients and healthy controls was conducted by real-time quantitative polymerase chain reaction (PCR). Results: TCGA database demonstrated that miR-101 was down-regulated in cervical cancer tissues compared with normal cervical tissues, and univariate Cox regression analysis indicated that decreased miR-101 expression was a highly significant negative risk factor. Similar trend was found in the serum miR-101. Serum level of miR-101 was associated with International Federation of Gynecology and Obstetrics (FIGO) stage (p=0.003), lymph node metastasis (p=0.001), and serum squamous cell carcinoma antigen (SCC-Ag) level >4 (p=0.007). The overall survival time of cervical cancer patients with a higher level of serum miR-101 was significantly longer than that of patients with a lower level of serum miR-101. Moreover, multivariate Cox regression analysis indicated that the down-regulated serum level of miR-101 was an independent predictor for the unfavorable prognosis of cervical cancer. Conclusion: Serum level of miR-101 is closely associated with metastasis and prognosis of cervical cancer; and, hence could be a potential biomarker and prognostic predictor for cervical cancer.

Antitumor Constituents from Anthriscus Sylvestris (L.) Hoffm

Chen, Hui,Jiang, He-Zhong,Li, Yong-Chao,Wei, Guo-Qing,Geng, Yun,Ma, Chao-Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Bioassay-guided chemical investigation of the roots of Anthriscus sylvestris (L.) Hoffm. resulted in the isolation of nine compounds, whose structures were determined by spectroscopic methods. Compound 1 was isolated from this plant for the first time and compounds 3 and 9 were first found from this genus. Different polar fractions of A. sylvestris extract and compounds 1, 6-8 and 9 were evaluated for antitumor activities against HepG2 (human hepatocellular carcinoma), MG-63 (human osteosarcoma cells), B16 (melanoma cells) and HeLa (human cervical carcinoma cells) lines by the MTT method. The petroleum ether fraction of A. sylvestris extract exhibited excellent inhibitory activity with an $IC_{50}$ value of $18.3{\mu}g/ml$. Among the isolates from the petroleum ether fraction, compound 7 showed significant inhibition against the growth of the four tumor cells with $IC_{50}$ values ranging from $12.2-43.3{\mu}g/ml$.

<i>Yersinia pseudotuberculosis</i> Exploits CD209 Receptors for Promoting Host Dissemination and Infection

He, Ying-Xia,Ye, Cheng-Lin,Zhang, Pei,Li, Qiao,Park, Chae Gyu,Yang, Kun,Jiang, Ling-Yu,Lv, Yin,Ying, Xiao-Ling,Ding, Hong-Hui,Huang, Hong-Ping,Mambwe Tembo, John,Li, An-Yi,Cheng, Bing,Zhang, Shu-Sheng American Society for Microbiology 2019 Infection and immunity Vol.87 No.1

<P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals.</P><P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade the small intestine via Peyer’s patches to initiate dissemination. In this study, we demonstrate that <I>Y. pseudotuberculosis</I> utilizes its lipopolysaccharide (LPS) core to interact with CD209 receptors, leading to invasion of human dendritic cells (DCs) and murine macrophages. These <I>Y. pseudotuberculosis</I>-CD209 interactions result in bacterial dissemination to MLNs, spleens, and livers of both wild-type and Peyer’s patch-deficient mice. The blocking of the <I>Y. pseudotuberculosis</I>-CD209 interactions by expression of O-antigen and with oligosaccharides reduces infectivity. Based on the well-documented studies in which HIV-CD209 interaction leads to viral dissemination, we therefore propose an infection route for <I>Y. pseudotuberculosis</I> where this pathogen, after penetrating the intestinal mucosal membrane, hijacks the <I>Y. pseudotuberculosis</I>-CD209 interaction antigen-presenting cells to reach their target destinations, MLNs, spleens, and livers.</P>

Relationships of Low Serum Levels of Interleukin-10 With Poststroke Anxiety and Cognitive Impairment in Patients With Clinical Acute Stroke

Zhao-jian Ying,Yuan-Yuan Huang,Meng-Meng Shao,Chu-Huai Chi,Ming-Xia Jiang,Yi-Hui Chen,Yu-Chen,Miao-Xuan Sun,Yan-Yan Zhu,Xianmei Li 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.3

Background and Purpose The relationships among interleukin (IL)-10 levels, anxiety, and cognitive status after stroke remain controversial. We aimed to determine the associations of serum IL-10 levels with poststroke anxiety (PSA) and poststroke cognitive impairment (PSCI). Methods We recruited 350 patients with stroke, of whom only 151 completed a 1-month follow- up assessment. The Mini Mental State Examination (MMSE) and Hamilton Anxiety Scale (HAMA) were used to assess the cognitive status and anxiety, respectively. Serum IL-10 levels were measured within 24 hours of admission. Results IL-10 levels were significantly lower in the PSA group than in the non-PSA group, and they were negatively associated with HAMA scores (r=-0.371, p<0.001). After adjusting for all potential confounders, IL-10 levels remained an independent predictor of PSA (odds ratio=0.471, 95% confidence interval=0.237–0.936, p=0.032). IL-10 levels were strongly correlated with behavior during interviews, psychic anxiety, and somatic anxiety. Patients without PSCI had higher IL-10 levels were higher in non-PSCI patients than in PSCI patients, and they were positively associated with MMSE scores in the bivariate correlation analysis (r=0.169, p=0.038), and also with memory capacity, naming ability, and copying capacity. However, IL-10 did not predict PSCI in the univariable or multivariable logistic regression. Conclusions Low IL-10 levels were associated with increased risks of PSA and PSCI at a 1-month follow-up after stroke. Serum IL-10 levels may therefore be helpful in predicting PSA.

Two New Monoterpene Alkaloid Derivatives from the Roots of Incarvillea arguta

Jian Jun Fu,Hui Zi Jin,Jiang Jiang Qin,Qi Zeng,Ying Huang,Wei Dong Zhang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.2

Two new monoterpene alkaloid derivatives, incargutosines C (1) and D (2), together with a known compound, argutine A (3) have been isolated from the roots of Incarvillea arguta Royle. The structures of 1 and 2 were established on the basis of 1D and 2D NMR spectroscopic analysis, while the absolute configurations of 1 and 2 were determined by a synthetic method. In addition, the cytotoxicity of 3 was evaluated using four tumor cell lines, A549, LOVO, 6TCEM, and MDA-MB-435 (MDA) by the MTT assay.

Analysis of Cancer Incidence in Zhejiang Cancer Registry in China during 2000 to 2009

Du, Ling-Bin,Li, Hui-Zhang,Wang, Xiang-Hui,Zhu, Chen,Liu, Qing-Min,Li, Qi-Long,Li, Xue-Qin,Shen, Yong-Zhou,Zhang, Xin-Pei,Ying, Jiang-Wei,Yu, Chuan-Ding,Mao, Wei-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Objective: The Zhejiang Provincial Cancer Prevention and Control Office collected cancer registration data during 2000 to 2009 from 6 cancer registries in Zhejiang province of China in order to analyze the cancer incidence. Methods: Descriptive analysis included cancer incidence stratified by sex, age and cancer site group. The proportions and cumulative rates of 10 common cancers in different groups were also calculated. Chinese population census in 1982 and Segi's population were used for calculating age-standardized incidence rates. The log-linear model was used for fitting to calculate the incidence trends. Results: The 6 cancer registries in Zhejiang province in China covered a total of 60,087,888 person-years during 2000 to 2009 (males 30,445,904, females 29,641,984). The total number of new cancer cases were 163,104 (males 92,982, females 70,122). The morphology verified cases accounted for 69.7%, and the new cases verified only by information from death certification accounted for 1.23%. The crude incidence rate in Zhejiang cancer registration areas was $271.5/10^5$ during 2000 to 2009 (male $305.41/10^5$, female $236.58/10^5$), age-standardized incidence rates by Chinese standard population (ASIRC) and by world standard population (ASIRW) were $147.1/10^5$ and $188.2/10^5$, the cumulative incidence rate (aged from 0 to 74) being 21.7%. The crude incidence rate was $209.6/10^5$ in 2000, and it increased to $320.20/10^5$ in 2009 (52.8%), with an annual percent change (APC) of 4.51% (95% confidence interval, 3.25%-5.79%). Age-specific incidence rate of 80-84 age group was achieved at the highest point of the incidence curve. Overall with different age groups, the cancer incidences differed, the incidence of liver cancer being highest in 15-44 age group in males; the incidence of breast cancer was the highest in 15-64 age group in females; the incidences of lung cancer were the highest in both males and females over the age of 65 years. Conclusions: Lung cancer, digestive system malignancies and breast cancer are the most common cancers in Zhejiang province in China requiring an especial focus. The incidences of thyroid cancer, prostate cancer, cervical cancer and lymphoma have increased rapidly. Prevention and control measures should be implemented for these cancers.

Disorders of Small and Large Intestine : Colorectal Cancer In Guangdong Province: A Demographic And Anatomic Survey

( An Gao Xu ),( Bo Jiang ),( Zhi Jin Yu ),( Xin Ying Wang ),( Xu Hui Zhong ),( Ji Hong Liu ),( Li Shou Xiong ),( Qiu Yun Luo ),( Ai Hua Gan ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Colorectal cancer is the third leading incidence of malignant tumour in the world and the incidence of colorectal cancer has steadily been increasing in Asia in recently years. The aim of our study is to determine the basic demographic features of patients with colorectal cancer and the anatomic distribution and characteristics of the tumour in Guangdong population. Methods: A review of patients from 1990 to 2004 at five hospitals was conducted, including Peal Triangle Area in Guangdong (Nanfang Hospital and Huizhou Central People`s Hospital), North area of Guangdong (Shaoguan North-Guangdong People`s Hospital), West area of Guangdong (Affiliated Hospital Guangdong Medical Institute) and East area of Guangdong (Meizhou People`s Hospital). Results: Analysis was carried out on 6,501 patents, only 6,488 cases provided age. The age ranged from 5 years old to 91 years old and the mean age of 6,488 cases is 59. 5.2% (340/6,488) of the patients was young CRC patients. The peak incidence was between the ages 61 and 70 years old (29.5%). The mean age increases from 55 years old (1990-1992) to 61 years old (2002-2004) and the proportion of young CRC patients descends from 7.1% to 3.5%. The make to female ratio is 1.5:1 and the ratio increased with age increasing. Of 6,501 lesions, 3,423 (52.7%) were located in rectum and 3,078 (47.3%) in colon, the ratio of rectum cancer to colon cancer is 1.1:1. The proportion of rectum cancer decreased significantly from 74.5% (1990-1992) to 64.9% (2002-2004) and that of the right sides colon cancer increased from 25.5% to 35.1%. In four different areas, the mean age of CRC increasing and East area of Guangdong ranked the fist. There was no significant difference in the ratio of male to female. Conclusions: The demography of colorectal cancer in Guangdong is different from before and further study should be pursued to find the reason.