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Wu Szu-Hsien,Yu Jin-Huei,Liao Yu-Ting,Liu Kuo-Hao,Chiang En-Rung,Chang Ming-Chau,Wang Jung-pan 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3
BACKGROUND: Infant adipose-derived mesenchymal stem cells (ADSCs) collected from excised polydactyly fat tissue, which was surgical waste, could be cultured and expanded in vitro in this study. In addition, the collecting process would not cause pain in the host. In this study, the proliferation, reduction of senescence, anti-oxidative ability, and differentiation potential in the infant ADSCs were compared with those in the adult ADSCs harvested from thigh liposuction to determine the availability of infant ADSCs. METHODS: Proliferation was determined by detecting the fold changes in cell numbers and doubling time periods. Senescence was analyzed by investigating the age-related gene expression levels and the replicative stress. The superoxide dismutase (SOD) gene expression, adipogenic, neurogenic, osteogenic, and tenogenic differentiation were compared by RTqPCR. The chondrogenic differentiation efficiency was also determined using RT-qPCR and immunohistochemical staining. RESULTS: The proliferation, SOD (SOD1, SOD2 and SOD3) gene expression, the stemness-related gene (c-MYC) and telomerase reverse transcriptase of the infant ADSCs at early passages were enhanced compared with those of the adults’. Cellular senescence related genes, including p16, p21 and p53, and replicative stress were reduced in the infant ADSCs. The adipogenic genes (PPARc and LPL) and neurogenic genes (MAP2 and NEFH) of the infant ADSC differentiated cells were significantly higher than those of the adults’ while the expression of the osteogenic genes (OCN and RUNX) and tenogenic genes (TNC and COL3A1) of both demonstrated opposite results. The chondrogenic markers (SOX9, COL2 and COL10) were enhanced in the infant ADSC differentiated chondrogenic pellets, and the expression levels of SODs were decreased during the differentiation process. CONCLUSION: Cultured infant ADSCs demonstrate less cellular senescence and replicative stress, higher proliferation rates, better antioxidant defense activity, and higher potential of chondrogenic, adipogenic and neurogenic differentiation.
Effects of Acupuncture at Pai-Hui on the Deficit of Memory
Chang, Yung-Hsien,Hsieh, Ming-Tsuen,Wu, Chi-Rei 경희대학교 2001 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.2001 No.1
Acupuncture has been introduced as one of the complementary therapies in traditional Chinese medicine. In addition to the analgesic effect, acupuncture also has anti-inflammatory, anti-allergic and immunomodulatory effects. Activation of the Bai-Hui by acupuncture was further shown to correct loss of consciousness, as well as to relieve mental abstraction, sluggish speech and hysteria. In this study, we investigated the attenuating effect of Pal-Hui acupuncture on scopolamine (SCOP)-induced or cycloheximide (CXM)-induced impairment of the passive avoidance response in rats. Our preliminary results indicated that acupuncture at Pal-Hui before or immediately after the training trial could significantly attenuate SCOP-induced impairment in rats. The effects were better than those treated before the right-room retention trial. Interestingly, the identical procedure could also significantly attenuate the CXM-induced impairment, but not those treated before the retention trial. The correction effect of acupuncture on CXM-induced impairment was equivalent to that of piracetam treatment. Acupunctures at Pal-Hui 15,30 or 60 min before the training trial could he all effective to attenuate CXM-induced impairment, and its efficacy paralleled acupuncture duration. Furthermore, we demonstrated the mechanism of the attenuating effects of acupuncture at Pal-Hui on CXM-induced memory consolidation deficits by combined with SCOP or p-chloroamphetamine(PCA). The attenuating effects of acupuncture at Pal-Hui was significantly antagonized by PCA but not SCOP. These results suggest acupuncture at Pal-Hui might mainly affect the memory storage process and had preventive and immediate therapeutic effects on the CXM-induced impairment and its efficacy paralleled acupuncture duration. The preventive effect of acupuncture at Pal-Hui on CXM-induced impairment is significantly reduced by serotonergic 5-HT releaser. However, the detailed mechanism of acupuncture on memory function and the differential mechanism of acupuncture on memory function an analgesia must be further investigated.
Performance Analysis of Permanent Magnet Brushless Motors for Mobility Scooters
Yu-Chuan Lu,Ming-Yang Cheng,Chun-Hsien Wu,Wei-Chih Chen,Cheng-Hu Chen 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5
Most modern commercial electric scooters are still equipped with the DC brush motor. This type of motor has mechanical structures such as permanent magnet, brush, and commutator. Therefore, it has attractive features, e.g., large start-up torque and easy to control. However, this kind of mechanical commutation often leads to drawbacks such as low power density, low reliability, and poor heat dissipation. In contrast, the Permanent Magnet Brushless Motors (PMBLMs) have advantages such as high efficiency and maintenance free, etc. Hence, the PMBLMs have great potential in becoming the actuators for electric scooters. In this paper, the performance of the PMBLMs with trapezoidal and sinusoidal back-emf waveforms driven by square wave commutation and sinusoidal commutation is investigated. To reduce the cost, low-resolution Hall sensors are used to implement sinusoidal commutation.
Effects of UV Assistance on the Properties of Al-Doped ZnO Thin Films Deposited by Sol-Gel Method
Yung-Kuan Tseng,Feng-Ming Pai,Yan-Cheng Chen,Chao-Hsien Wu 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.6
We report here the preparation of aluminum doped zinc oxide transparent conductive thin films by a UV-assisted sol-gel method. It was found that UV irradiation creates ozone, which promotes the conductivity and transparency of the films. Boro-silicate glasses are used as substrates; an PGME is used as a solvent; after spin-coating,the films are dried and radiated with UV and then heated to 400°C for decarburization and 500°C for annealing under air. The surface morphologies of the prepared films are observed by FE-SEM and AFM. It was found that the films irradiated with UV-C are smoother and denser. An XRD analysis shows that the films have a typical wurtzite crystalline structure with a c-axis orientation normal to the surface. The electric resistance values measured with a four-point probe show that the films irradiated with UV have better conductivity (at approximately 3.4 × 10−3Ω-cm) than the films that did not undergo UV irradiation. An analysis by visible light spectrometry indicates that the AZO films irradiated with UV are more transparent than the films without UV-irradiation.
( Yi Chia Lee ),( Tsung Hsien Chiang ),( Jyh Ming Liou ),( Hsiu Hsi Chen ),( Ming Shiang Wu ),( David Y Graham ) 대한간학회 2016 Gut and Liver Vol.10 No.1
Although the age-adjusted incidence of gastric cancer is declining, the absolute number of new cases of gastric cancer is increasing due to population growth and aging. An effective strategy is needed to prevent this deadly cancer. Among the available strategies, screen-and-treat for Helicobacter pylori infection appears to be the best approach to decrease cancer risk; however, implementation of this strategy on the population level requires a systematic approach. The program also must be integrated into national healthcare priorities to allow the limited resources to be most effectively allocated. Implementation will require adoption of an appropriate screening strategy, an efficient delivery system with a timely referral for a positive test, and standardized treatment regimens based on clinical efficacy, side effects, simplicity, duration, and cost. Within the population, there are subpopulations that vary in risk such that a "one size fits all" approach is unlikely to be ideal. Sensitivity analyses will be required to identify whether the programs can be utilized by heterogeneous populations and will likely require adjustments to accommodate the needs of subpopulations. (Gut Liver 2016;10:12-26)