배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

Taxol합성에 관한 연구. Ⅱ : 3-(2-Hydroxyethyl)-2, 2, 4-trimethyl-3-cyclohexenone SEM ether의 합성

임남수,신동수,정윤성,김정주,박두천,주우홍 昌原大學校 基礎科學硏究所 1995 基礎科學硏究所論文集 Vol.7 No.-

3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-cyclohexenone SEM ether ??, an intermediate in the synthesis of taxol was provided in eight steps. The keto ketal ?? was obtained in three steps from 1,3-cyclohexanedione. Reaction of the potassium enolate of ?? with N-phenyltrifluoromethanesulfonimide provided triflate ?? in 84% yield. Thus palladium-mediated cross-coupling reaction of enol triflate ?? with vinyltributylstannane afforded diene ?? in a 89% yield. 3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-Cyclohexenone SEM ether was prepared from diene ?? by sequential hydroboration [9-BBN, THF, 89%], deprotection[pTsOH ·H₂O, THF-H₂O,~100%], and silyation [SEMC], iPr₂NEt, nBu₄NI, CH₂Cl₂, 94%]

乳牛의 血液性狀과 泌乳量에 關한 硏究

林貞澤,金宇權,李載洪,韓邦根 全南大學校農漁村開發硏究所 1982 農業科學技術硏究 Vol.17 No.2

國內에서 飼育되고 있는 Holstein種의 血液成分値 그리고 血液成分値와 泌乳量과의 相關關係등을 檢討할 目的으로 全南地域에서 飼育되고 있는 外觀上 健康하다고 認定된 Holstein種 870餘頭를 對象으로 血液成分値를 調査檢討 하였고 한편 産乳量을 調査하여 血液成分値와 産乳量과의 關係를 檢討하여 다음과 같은 結論을 얻었다. 1. 平均 RBC數는 5.97(3.20~9.96)×10 6/㎕이었으나 成牝牛의 平均 RBC數는 6.06(3.20~9.74)×10 6/㎕이었으며 年齡別 平均 RBC數에서 1年牛가 5.19×l0 6/㎕로 特히 낮았다. 2. 平均 Hb量은 9.61(5.3-18.4)g/100ml 이었으나 成牝牛의 平均 Hb量은 9.42(5.3-18.4)g/100ml 이었으며 年齡別 平均 Hb量에서 2年牛가 8.97g/100ml로 特히 낮았다. 3. 平均 PCV値는 31.10(19~5)ml/100ml이었으며 成牝牛의 平均 PCV値는 31.04(19~55)ml/100ml 이었으며 年齡別 平均 PCV値에서 2年牛가 29.84ml/100ml로 가장 낮았다. 4. 平均 MCV値는 52.09(23.77~86.57) fl이었으나 成牝牛의 平均 MCV値는 51.22(29.77~86.57)fl 이었으며 1年未滿牛의 55.64fl 및 1年牛의 57.63fl 등 송아지에서 더 높았다. 5. 平均 MCH値는 16.10(8.21~28.96)pg 이었으며 成牝牛의 平均 MCH値는 15.54(8.21~28.96)pg 이었으며 1年未滿牛의 18.41pg 및 1年牛의 19.17pg 등 송아지에서 더 높았다. 6. 平均 MCHC値는 30.90(17.35~53.75)g/100ml 이었으나 成牝牛의 平均 MCHC値는 30.35(17.35~53.57)g/100ml 이었으며 1年未滿牛의 33.27g/100ml 등 송아지에서 더 높았다. 7. 貧血牛는 大部分이 大赤血球性이며 高血色素性이었으나 小赤血球性(MCV 37.15fl)이며 低血色素性(MCH 12.35pg)인 個體, 大赤血球性이며 低血色素性(MCHC 24.21g/100ml)인 個體 그리고 小赤血球性(MCV 35.71fl)이며 高血色素性(MCHC 40.83g/100ml)을 나타낸 個體도 發見되었다. 8. 平均 WBC數는 10.186(3,200~31,600)㎕ 이었고 成牝牛의 平均 WBC數는 10,408(3,200~31,600)㎕으로 어느 年齡層에서나 높은 値이었으나 2年牛가 12,647/㎕로 特히 높았다. 9. WBC鑑別計數의 平均値는 淋巴球가 66.95(36~91), 單核球가 3.08(0~12), 好中球가 25.69(2~54), 好酸球가 3.84(0~18) 그리고 好鹽基球가 0.44(0~5)%이었으나 成牝牛의 平均値는 淋巴球가 65.87(36~86), 單核球가 3.15(0~9), 好中球가 25.84(6~53), 好酸球가 4.73(0~15) 그리고 好鹽基球가 0.4(0~2)% 이었다. 10. 淋巴球는 1年牛를 頂点으로 年齡의 增加에 따라서 減少하고 있었으나 好中球는 1年牛가 가장 낮았으며 年齡의 增加에 따라서 增加하여 淋巴球와는 負의 相關關係를 나타냈고 單核球와 好酸球는 高齡牛에서 若干 높은 傾向이 있었다. 11. 平均血小板數는 252,429(93,000~964,000)/㎕이었으나 成牝牛의 平均血小板數는 245,658(110,000~570,000)/㎕이었으며 年齡別特徵은 認定할 수 없었다. 12. 平均血淸TP量은 6.88(4.10~10.20)g/100ml이었으나 成牝牛의 平均血淸TP量은 7.35(5.10~10.20)g/100ml이었으며 年齡의 增加에 따라서 점점 增加하는 傾向이 있었다. 13. 平均血淸Alb量은 3.43(2.00~4.40)g/100ml이었으나 成牝牛의 平均血淸Alb量은 3.51(2.00~4.40)g/100ml이었으며 1年未滿牛의 3.33g/100ml 및 1年牛의 3.31g/100ml등 송아지에서 若干 더 낮은 傾向이 있었으나 그 外는 거의 비슷하였다. 14. 平均血淸Glb量은 3.45(1.10~7.15)g/100ml이었으나 成牝牛의 平均血淸Glb量은 3.84(1.40~7.15)g/100ml이었으며 年齡의 增加에 따라서 점점 增加하는 傾向이 있었다. 15. 平均血淸A/G比는 0.99(0.40~3.33)이었으나 成牝牛의 平均血淸A/G比는 0.91(0.43~2.64)이었으며 年齡의 增加에 따라서 점점 減少하는 傾向이 있었다. 16. 平均血淸 cholesterol量은 145.65(44.0~336.0)mg/100ml이었으나 成牝牛의 平均血淸cholesterol量은 180.93(46.4~336.0)mg/100ml이었으며 年齡의 增加에 따라서 增加하여 4年牛를 頂点으로 다시 下向하는 傾向을 나타내고 있었다. 17. 平均血淸 glucose量은 46.00(27.0~96.0)mg/100ml이었으나 成牝牛의 平均血淸glucose量은 39.61(27.0~65.0)mg/100ml이었다. 血淸 glucose量은 全般的으로 낮은 値이었으나 年齡別 平均血淸glucose量에서 1年未滿牛가 58.07(39.7~96.0)mg/100ml로 가장 높았으며 特히 어린 송아지에서 높았다. 18. 平均血淸Mg量은 2.68(0.20~8.60)mg/100ml이었으나 成牝牛의 平均血淸Mg量은 2.72(0.20~8.46)mg/100ml이었으며 年齡別 平均血淸Mg量에서 7年以上牛가 2.27mg/100ml로 特히 낮았으며 老齡牛에서 Mg의 減少가 顯著하였다. 19. 平均血淸Ca量은 4.36(3.16~8.40)mEq/L이었고 成牝牛의 平均血淸Ca量도 4.37(3.16~8.40)mEq/L로 全般的으로 낮은 量이었으며 年齡別 特徵은 認定할 수 없었다. 20. 平均血淸P量은 6.27(3.10~9.78)mg/100ml이었으나 成牝牛의 平均血淸P量은 5.92(3.10~9.78)mg/100ml이었으며 年齡別 平均血淸P量에서 1年未滿牛가 7.50(4.70~9.40)mg/100ml로 特히 높았다. 21. 平均血淸Ca/P比는 1.39(0.68~2.64)이었으나 成牝牛의 平均血淸Ca/P比는1.48(0.68~2.64)로 全般的으로 낮은 値이었으며 年齡別 平均血淸Ca/P比에서 1年未滿牛가 1.21(0.83~1.93)로 가장 낮았다. 22. 平均血淸K量은 4.94(3.5~6.7)mEq/L이었으나 成牝牛의 平均血淸K量은4.84(3.5~6.2)mEq/L이었으며 年齡別 血淸K量에서 1年未滿牛가 5.22(4.0~6.7)mEq/L로 特히 높았으며 그外는 거의 비슷하였다. 23. 平均血淸Na量은 138.43(123~157)mEq/L이었으나 成牝牛의 平均血淸Na量은137.32(123~157)mEq/L이었으며 1年未滿牛의 139.74mEq/L 및 1年牛의 140.16mEq/L등 송아지에서 더 높았다. 24. 平均血淸Cl量은 99.96(84.6~123.0)mEq/L이었고 成牝牛의 平均血淸Cl量도 100.09(84.6~123.0)mEq/L로 年齡別差異点은 발견할수 없었다. 25. 平均血淸Na/K比는 28.02(21.49~37.14)이었으나 成牝牛의 平均血淸Na/K比에서 1年未滿牛가 26.77(21.49~35.00)로 特히 낮았다. 26. RBC數, Hb數, PCV値, MCH 및 MCHC는 모두 15~20kg群에서 第一 높았고 20~25kg群, 25kg以上 群으로 갈수록 낮아지는 傾向을 보였다. 卽 赤血球系 檢査値와 産乳量과는 負의 相關關係에 있는 것으로 보아진다. 27. WBC數와 産乳量과는 負의 相關關係가 있는 것으로 보아지며 血小板數와 産乳量과는 特別한 關係를 認定할 수 없었다. 28. WBC鑑別系數와 産乳量과의 相關에서는 淋巴球는 負의 相關關係에 있는 것으로 보아지며 好中球는 淋巴球와는 反對로 正의 相關關係에 있는 것으로 보아진다. 한편 單核球와 好酸球는 産乳量增加에 따라서 增加하는 傾向이 있었다. 29. 血淸TP量 및 血淸Glb量은 産乳量增加에 따라서 增加하는 傾向이 있었으며 血淸Alb量 및 血淸A/G比는 産乳量增加에 따라서 減少하는 傾向이 있었다. 30. 血淸glucose量과 産乳量과는 負의 相關關係에 있는 것으로 보아지며 血淸cholesterol量은 産乳量增加에 따라서 增加하는 傾向이 있었다. 31. 血淸Mg量은 産乳量이 가장 많은 25kg以上群에서 特히 낮았으며 血淸Ca 및 P量은 産乳量增加에 따라서 增加하는 傾向이 있었으나 血淸Ca/P比는 産乳量增加에 따라서 減少하는 傾向이 있었다. 32. 血淸Na量 및 血淸Na/K比는 産乳量과는 正의 相關關係에 있는 것으로 보아지며 血淸K量은 産乳量增加에 따라서 減少하는 傾向이 있었고 血淸Cl量과 産乳量과는 特別한 關係를 認定할 수 없었다. This work was conducted to determine the composition of the blood of Holstein cattle in Korea and the relationships between the blood composition and milk production. Blood samples were taken from 870 healthy cattle in Chonnam province. Milk yield was recorded, and the results obtained in this study are summarized as follows: 1. Mean red blood cell(RBC) count was 5.97(3.20~9.96)×10 6/㎕, whereas the RBC count of adult cows was 6.06(3.20~9.74)×10 6/㎕. The RBC count between age groups appeared to be especially lower in the age group of 1 year(5.19×l0 6/㎕) than in the other age groups. 2. Mean hemoglobin(Hb)content was 9.61(5.3-18.4)g/100ml, whereas the Hb content of adult cows was 9.42(5.3-18.4)g/100ml. The Hb content between age groups appeared to be especially lower in the age group of 2 years(8.97g/100ml) than in the other age groups. 3. Mean packed cell volume(PCV) was 31.10(19-5)ml/100ml. The PCV between age groups was lowest in the age group of 2 years(29.84ml/100ml). 4. Mean corpuscular volume(MCV) was 52.09(23.77-86.57) fl. whereas the MCV of adult cows 51.22(29.77-6.57) fl. Mean corpuscular hemoglobin(MCH) was 16.10(8.21-28.96) pg, whereas the MCH of adult cows was 15.54(8.21-28.96) pg. Mean corpuscular hemoglobin concentration(MCHC) was 30.90(17.35-53.57)g/100ml, whereas MCHC of adult cows was 30.35(17.35-53.57)g/100ml. The MCV, MCH and MCHC appeared to be higher in the calves and heifers than in the adult cows. 5. Most anemic cattle showed macrocytic, hyperchromic anemia. Various types of anemia were detected; microcytic(MCV 37.15 fl) hypochromic(MCH 12.35.pg); macrocytic hypochromic(MCHC 24.21g/100ml); microcyticfMCV 35.71 fl)hyperchromic(MCHC 40.83g/100ml). 6. Mean white blood cell(WBC) count was 10,186(3,200-31,600)/ l, which was high compared with other results. The WBC count between age groups appeared to be especially higher in the age group of 2 years(12, 647/yu.e) than in the other age groups. 7. Mean differential WBC count was lymphocyte 66.95(36-91), monocyte 3.08(0-12), neutrophil 25.69(2-54), eosinophil 3.84(0-18) and basophil 0.44(0-5%), respectively, while in the adult cows, it was lymphocyte 65.87(36-86), monocyte 3.15(0-9), neutrophil 25.84(6-53), eosinophil 4.73(0-15) and basophil 0.41(0-2)%, respectively. Lymphocyte count increased up to 1 year of age and thereafter it decreased with age, whereas the reverse was the case with neutrophil count. There was a negative correlation between lymphocyte and neutrophil counts. Monocyte and eosinophil counts tended to be slightly higher in the older age groups. 8. Mean platelet count was 252,429(93,000-964,000)/㎕; no differences were found in the platelet count between age groups. 9. Mean serum total protein(TP) content was 6.88(4.10-10.20)g/100ml, while the TP content of adult cows was 7.35(5.10-10.20)g/100ml. The TP content tended to increase with age. 10. Mean serum aIbumin(Alb) content was 3.43(2.00-4.40)g/l00ml, while the Alb content of adult cows was 3.51(2.00-4.40)g/100ml. The Alb content appeared to be lower in the calves and heifers than in the adult cows. 11. Mean serum globulin(GIb) content was 3.45(1.10-7.15)g/100ml, while the Gib content of adult cows was 3.84(1.40-7.15g)/100ml. The Gib conienc tended to increase with age. 12. Mean serum .albumin: globuIin(A/G) ratio was 0.99(0.40-3.33), while the A/G ratio of adult cows was 0.91(0.43-2.64). The A/G ratio tended to decrease with age. 13. Mean serum cholesterol concentration was 145.65(44.0-336.0)mg/100ml, whereas the scrum cholesterol concentration of adult cows was 180.93(46.4-336.0)mg/100ml. The serum cholesterol concentration increased up to 4 years of age and thereafter it decreased with age. 14. Mean serum glucose concentration was 46.00(27.0-96.0)mg/100ml, whereas the serum glucose concentration of adult cows was 39.61(27.0-65.0)mg/100ml, which was low compared with other results. The serum glucose concentration between age groups appeared to be especially higher in the age group of less than 1 year(58.07mg/100ml) than in the other age groups. 15. Mean serum magnesium(Mg) concentration was 2.68(0.20-8.60)mg/100ml, whereas the Mg concentration of adult cows was 2.72(0.20-8.46)mg/100ml. The Mg concentration between age groups appeared to be especially lower in the age group of more than 7 years(2.27mg/100ml) than in the other age groups. Decreased Mg concentration was shown in older cows. 16. Mean serum calcium(Ca) concentration was 4.36(3.16-8.40)mEq/L, which was low compared with other results. No differences were found in the Ca concentration between age groups. 17. Mean serum inorganic phosphate(P) concentration was 6.27(3.10-9.78)mg/100ml, whereas the P concentration of adult cows was 5.92(3.10-9.78)mg/100ml. The P concentration between age groups appeared to be especially higher in the age group of less than 1 year(7.50mg/100ml) than in the other age groups. 18. Mean serum calcium: inorganic phosphate(Ca/P) ratio was 1.39(0.68--2.64). The Ca/P ratio of adult cows was 1.48(0.68-2.64), which was lower compared with other results. The Ca/P ratio between age groups was lowest in the age group of less than 1 year(1.21). 19. Mean serum potassium(K) concentration was 4.94(3.5-6.7)mEq/L> whereas the K concentration of adult cows was 4-84(3.5-6.2)mEq/L. The K concentration between age groups appeared to be especially higher in rhe age group of less than 1 year(5.22mEq/L) than =n the other age groups. 20. Mean serum sodiurn(Na) concentration was 138.413(123-157)mEq/L> whereas the Na concentration of adult cows was 137.32(123-157)mEq/L. The Na concentration between age groups appeared to be higher in the calves and heifers than in the adult cows. 21. Mean serum chloride concentration was 99.96(84.6-123.0)mEq/L; no differences were found between age groups in the scrum chloride concentration. 22. Mean serum sodium: potassium(Na/K) ratio was 28.02(21.49-37.14), whereas it was 28.37(22.00-37.14) in the adult cows. The Na/K ratio between age groups appeared to be especially lower in the age group of less than 1 year(26.77) than in the other age groups. 23. The values of RBC count, Hb content, PCV, MCH and MCHC increased up to 15-20kg milk yielding group and thereafter they tended to decrease with higher milk yielding groups. In other words, there was a negative correlation between the values of erythrocytic series and milk production. 24. The leukocyte and lymphocyte count were a negative correlation with milk yield, while the neutrophil count was a positive correlation wirh milk yield. The monocyte and eosinophil count tended to increase as milk production increased. No relationships were found between the platelet count and milk production. 25. The serum total protein and globulin content tended to increase as milk production increased, while the reverse was the case with serum albumin content and A/G ratio. 26. The serum glucose concentration was a negative correlation with milk yield. The Serum cholesterol concentration tended to increase as milk production increased. 27. The serum calcium and inorganic phosphate concentration tended to increase as milk production increased, while the opposite was the case with Ca/P ratio. The serum magnesium concentration was especially lower in the highest milk yielding group than in the other milk yielding groups. 28. The serum sodium concentration and the Na/K ratio tended to increase as milk production increased. In other words, there was a positive correlation with milk yield. The serum potassium concentration tended to decrease as milk production increased. No relationships were found between the serum chloride concentration and milk production.

信號交叉路의 交通特性과 左回轉 禁止에 따른 運營效果에 관한 硏究

홍익상,공창환,정우영,임채문 대구대학교 산업기술연구소 1993 産業技術硏究 Vol.12 No.-

This study analyzed the characteristics of the operation of left turns at intersections of the streets in Taegu City. The points of the study were as follows: increase of traffic capacity, decrease of delay time, and to do so this study aims at seeking a way for the most appropriate signal cycle and an effective way to operate intersections. The following are the results: 1st The arterials of Taegu City have a high rate of left turns, and yet are not divided into specific functions, which impairs the efficient use of the functions. It is required that a study be made on the way of utilizing the left turn to the maximum of the operational functions of signalized intersections. 2nd As traffic volume and left turn ratio increase, it was revealed that the optimum signal cycle and delay increased, adoption of efficient lanes was analysed to be most effective at the intersection which have a great of traffic volume and a high or rate of left turns. 3rd On the independent intersection in Taegu city, when left turn is prohibited, it may give poor approaches, but according to analyses this will bring a phenomenal decrease in the optimum signal cycle and delay.

유도전동기의 자속 및 토크 기울기에 의한 DTC에 관한 연구

임병옥,임홍우,정병호,이강연,백형래,조금배 조선대학교 에너지.자원신기술연구소 2003 에너지·자원신기술연구소 논문지 Vol.25 No.1

유니버셜모터의 저차 고조파전류 저감을 위한 속도제어기에 관한 연구

임홍우,박수강,백형래 조선대학교 에너지.자원신기술연구소 2000 에너지·자원신기술연구소 논문지 Vol.22 No.1

Universal motors are commonly used for portable tools and other application. The universal motors can be implied AC or DC voltage source. Phase-angle control AC drive systems gain a high popularity due to their simple implementation despite the disadvantage of their poor input power factor especially for large values of phase delay angle. Futhermore it contains a large amount of subharmonic current. But pulse width modulation controlled DC drive systems reduce these problems. This paper compared the harmonic characteristics of phase angle control AC system that is controlled by TCA785 with zero voltage crossing similar to traditional system and DC chopper system that is used in PWM controller.

슬관절 내측부 인대 손상의 보존적 치료

임홍철,심재학,남혁우,왕준호,노영진 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

Purpose : The medial collateral ligament injury is the most common injury of the knee. Recently Non-operative treatment has been preferred than operative treatment and most of patients have satisfactory clinical results after non-operative treatment. But a few patients have continuous instability after non-operative treatment, so the purpose of this study is to fine out the cause of persistent symptom. Methods and Materlals : We performed a retrospective study of one hundred thirty seven patients with medial collateral ligament injury treated non-operative from January 1990 to December 1997. W divided patients into two groups: isolated medial collateral ligament injury (groupⅠ): medial collateral ligament injury with concomitant anterior cruciate ligament injury (groupⅡ) and we analysed MRI finding, valgus instability, and Lysholm knee score. Result : At the follow up, three of group Ⅰ, five of group Ⅱ had persistent grade Ⅲ valgus instability. In group Ⅰ, we could find two case of rupture of the proper of medial collateral ligament with inward inversion and one case of concomitant posteromedial compartment injury. We could find the proper rupture of medial collateral ligament with inversion inward in two patients, the injury of posteromedial compartment in one patient. Two patients of group Ⅱ with grade Ⅲ valgus instability had anterior instability with laxity of grafted tendon after anterior cruciate ligament reconstruction. Conclusion : Most of isolated injury of the medial collateral ligament heals wthout operative intervention. But in case of rupture of the proper portion of medial collateral ligament with inversion inward or concomitant injury of posteromedial compartment, we should consider the operative treatment of medial collateral ligament. In case of medial collateral ligament injury with anterior cruciate ligament, it would better to consider the delicate reconstruction of anterior cruciate ligament.

축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.

여행속도에 따른 Q-V관계식 산정에 관한 연구

임채문,정우영,공창환,홍익상 대구대학교 과학기술연구소 1995 科學技術硏究 Vol.2 No.1

The purpose of this study is to estimate a suitable traveling speed model based on using the traffic data collected by test-vehicle traveling method and the data on road geometric and operating characteristics. The conclusions of this study are as follows. In travel speed model(Q-V), regression model (V=9.635+0.0169L - 0.00045Q - 2.1926N, R²=0.76) the important independent variables are speed is closely related with distance, traffic volume and the number of traffic lane, form the result of speed-flow regression analysis, link travel speeds are influenced by link distance, traffic volume, and the number of lanes. As expectedly by the most significant factor influencing link travel speeds. The changing of traveling speed according to section distance is appeared large. According to the result of analysis of travel speed and traffic density model(Q-K), as according the that density increases the travel speed is decreases, on the other hand travel speed increases, the density decreases. The speed equation (u=68.72e^(-k/22.6)) is derived by form the Q-K relationships founded form the study, traffic volume increases as traffic speed increase up to the critical traffic density. If traffic volume decreases when traffic density is abduce the critical traffic density. Using Q-V equation estimated by regression analysis and Q-K equation estimated by density, Traffic value test was accomplished by computer program(TRANPLAN). There were difference between too test, however, the deviation was small. On case of using program, the application extent we considered, is between 25Km/h - 60Km/h. Traffic volumes obtained from TRANPLAN are governably 10% larger than traffic volume estimated form Q-K model. However the difference of the critical traffic density bow Q-K model and TRANPLAN, are relatively small. It is desirable to reduce traffic volume by 0.7∼9% over all, speed range when TRAPLAN is applied in Q-K relationship.

PEMFC의 효율적 시뮬레이션을 위한 Matlab/Simulink와 PSIM기반의 Co-Simulation

임채홍,이우택 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

Fuel Cell system is expected to use widely in the near future. Proton Exchange Membrane Fuel Cell(PEMFC) system is broadly investigated due to low operating temperature and fast start up characteristics. To shorten the time and effort putting into development, the simulation procedure is needed, For Simulating Fuel Cell system, specified tools are necessary because Fuel Cell system consists of control system, power generation system and power conversion system. Matlab/Simulink is useful to simulate Fuel Cell's control algorithm and V-I characteristics. PSIM is useful to simulate Fuel Cell system's power conversions. This paper, focuses on Co-Simulation of PEMFC system using Matlab/Sumulink and PSIM. Co-Simulation is an effective method to simulate PEMFC's complicated characteristics.