Anti‑inflammatory effect of Rosa laevigata extract on in vitro and in vivo model of allergic asthma via the suppression of IgE and related cytokines

Seung‑Hyeon Lee,Seung‑Han Choi,In‑Seung Lee,Yumi Kim,Eun‑Jin An,Hyeung‑Jin Jang 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Backgrounds Exposure to a toxic stress environment leads to excessive inflammatory reactions and induces allergic asthma resulting in airway hyper-responsiveness. We investigated whether Rosa laevigata Michx. (RL) exhibits anti-inflammatory effects related allergic asthma in both an in vitro and in vivo studies. Methods To investigate the preventive effect of RL, A549 cells were pretreated with non-toxic doses of RL (500, 1000 μg/ mL) and induced by epidermal growth factor (EGF) (10 ng/mL). First, we evaluated cytotoxicity via a MTT assay. The inhibitory effects of NF-κB activity and COX-2 expression were confirmed by a western blot assay. In the in vivo study, BALB/c mice were challenged with regard to ovalbumin via an intraperitoneal injection of RL (50, 100 mg/kg) and were killed to collect bronchoalveolar lavage fluid, lung tissues and blood serum. The number of inflammatory cells, the secretion of IgE and related cytokines were monitored by ELISA and multiplex assays. Results RL significantly suppressed NF-κB activity and COX-2 expression levels in EGF-induced A549 cells. In a chronic inflammation mice model, pretreatment of RL attenuated allergic airway inflammation by reducing inflammatory cells, the secretion of IgE and related cytokines in a dose-dependent manner. Conclusions The results of this study present the possibility of RL as a therapeutic agent for allergic asthma patients via the suppression of IgE and related cytokines.

새로운 유사 원자층 증착 방법에 의한 산화막의 형성 및 터널링 자기 접합에의 응용

한신희,정원철,이장식,김병동,주승기 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.1

The tunneling barrier is crucial to the overall performance in magnetic tunnel junction. We have suggested a new formation method for the tunnel barrier, which has utilized pseudo atomic layer deposition with sputtering (PALDS). As is well known, all metallic thin films oxidize more or less under atmospheric conditions. With using this property of metallic thin films, we have prepared tunnel barrier by sputtering metallic thin film and exposing it to the oxygen ambience. From transmission electron microscopy (TEM) observation, the formed tunnel barrier has been confirmed to have clear and good interface between magnetic layers and tunnel barrier. From atomic force microscopy (AFM) observation, it has been also confirmed to have low surface roughness. The TMR MRAM cell manufactured using PALDS method has been shown to exhibit typical tunnel resistance of 86 KΩ·μm^2 and TMR ratio of about 40%. The 40% of MR is quite large. It is considered that this value is geometrically enhanced magnetoresistance due to the low junction resistance of MTJ.

림프절 질환들에서 병변에 따른 수지상 세포의 변화

장영우,김대중,양승하,김의한 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

The reaction patterns of the dendritic cells in lymph node lesions was as follows; 1. The number of the dendritic cells in the nonspecific lymphadenitis showed no significant decrease, so the immunologic reaction in the lymph nodes substained. 2. The number of the dendritic cells in tuberculous lymphadenitis showed significant decrease in case of the tubercles present in the lymph node than in case of no tubercle present. 3. The dendritic cells in lymph nodes of gastric adenocarcinoma were entirely disappeared regardless of metastasis. 4. The dendritic cells in lymph node of infiltrating ductal carcinoma were observed significantly regardless of metastasis. Above results suggested that the no expression of dentritic cells appeared to be the result of loss of function than disappearance. The loss of function of dendritic cells meaned that the easy infiltration of agents due to lack of immune reaction Because the immune reaction of lymph node in infiltrating ductal carcinoma was sustained while the immune reaction of lymph node in gastric adenocarcinoma was significantly reduced, the prognosis of infiltrating ductal carcinoma was better than gastric adenocarcinoma. But more research is necessary about the other prognostic factors.

편평 세포암, 광선 각화증 및 Bowen병에서 p53과 Ki-67의 발현 양상

장영우,양승하,김의한 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : The function of p53 protein was known to regulate damaged cell proliferation in S phase, so damaged cell proliferation was not occurred by apoptosis. Ki-67 nuclear antigen has been used as a marker to define growth fraction in both benign and malignant tumors. The Ki-67 nuclear antigen was expressed throughout the cell cycle except G0 and early G1 phase. The role of p53 and Ki-67 in oncogenesis, tumor progression and prognosis were important. The authors analyzed immunohistochemical expression of p53 and Ki-67 in squamous cell carcinoma, actinic keratosis and Bowen's disease. Method: Seventeen cases of squamous cell carcinoma, fifteen cases of actinic keratosi and seven cases of Bowen's disease were immunohistochemically stained with p53 and Ki-67 monoclonal antibodies. Results: 1. p53 protein were expressed mainly in basal layer in well differentiated squamous cell carcinoma and diffusely expressed in poorly differentiated squamous cell carcinoma. In actinic keratosis, the expression pattern of p53 was similar to well differentiated squamous cell carcinoma, but the intensity was lower. 2. In Bowen's disease, the p53 protein was more diffusely and strongly expressed than squamous cell carcinoma. 3. The ki-67 labelling index in squamous cell carcinoma was below 20% regardless of differentiation. In actinic keratosis, the labelling index was lower. 4. The Ki-67 labelling index in Bowen's disease was higher than squamous cell carcinoma and weakly expressed in basal layer, especially adjoining dermis. 5. In mitotic cells, the p53 protein was not expressed, but Ki-67 nuclear antige was strongly expressed. Above results suggested that Bowen's disease is almostly composed of immature cell while squamous cell carcinoma was composed of the mature and immature cells. In spite of higher labelling index in Bowen's disease, the growth rate is really faster in squamous cell carcinoma. The authors suggested the reason that the number of the mitotic cells in Bowen's disease were fewer than that of squamous cell carcinoma.

Plyometric Training 박스의 높이 차이가 남자 핸드볼 선수의 점프력에 미치는 영향

장창현,김승권,한주욱 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

Twenty four male middle school and high school handball players were involved an adapted Plyometric Training program which was composed of depth jump and box jump, 3 days a week for B weeks. The jump ability factor before and after the training was analyzed, and the following conclusions were drawn 1. The group trained with higher box showed greater increase in their vertical jump ability than the group trained with lower box for both middle and high school players. 2. The group trained with higher box showed greater increase in their vertical running jump ability than the group trained with lower box for both Middle and high school players. 3. The group trained with higher box showed greater increase in the ability of staying in the air than the group trained with lower box for both middle and high school players. 4. The middle school players showed greater increase in the jump ability than the high school students.

자궁 경부의 상피내암, 침윤이 있는 상피내암과 침윤성 편평상피암에서 p53, Ki-67 및 HER-2/neu의 표출 양상

장우섭,오미혜,양승하,김의한 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

To observe the expression of p53, Ki-67 and HER-2/neu in uterine cervical lesions, such as 10 cases of carcinoma in situ(CIS), 7 cases of carcinoma in situ with stromal invasion and 8 cases of invasive squamous cell carcinoma without carcinoma in situ, and to evaluate the corrleationship between results, the author has been carried out immunohistochemical staining with monoclonal antibodies to p53, Ki-67 and HER-2/neu. So, following results are obtained. 1. The p53 show negative reaction in all cases of CIS, and similar patterns in CIS with stromal invasion and invasive squamous cell carcinoma. 2. Ki-67 show high proliferation pattern in all cases and the basal cell show proliferating activity. 3. HER-2/neu show higher score in area of CIS than invasive area in stroma. 4. The stain pattern of HE-2/neu and p53 appeared to be inverse proportion to each other

Stearic Acid로 被覆한 Fe₃O₄에 의한 油分除去 (Ⅰ)

韓尙敏,張哲鉉,朴勝祚 동아대학교 환경문제연구소 1989 硏究報告 Vol.12 No.1

The large quantity of iron contained in waste sludge discharged form pickling liquor in a steel industry was used for Fe₃O₄. Then we new adsorbent for oil removal was made. The adsorbent was made of Fe₃O₄ coated with stearic acid. The results of this study that perform batch test to relate adsorptive ability, and oil removal efficiency of the adsorbent with kerosene, bunker-c oil, and emulsified bunker-c oil are followings : 1) The results of batch adsorption test fit well to the Freundlich equation, and the K of kerosene, bunker-c oil, and emulsified bunker-c oil was, 0.20, 0.17, and 0.16, respectively. 2) The adsorptive reaction was the first order reaction and the K' was 0.526/min. 3) The kerosene removal efficiency by new adsorbent was 30% in the alkalinity, 85% in the acidity and 92% in a neutrality.

신장기증자들의 심리적 특성

정승아,김재환,강종명,이장한,김경욱 漢陽大學校 精神健康硏究 1998 精神健康硏究 Vol.17 No.-

새열기형의 임상적 고찰

김장묵,김대식,백승혁,한동렬,김희중,백병준,오천환 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Background and Objectives : Branchial cleft anomalies are encountered rarely in clinical practice. We reviewed medical records of branchial cleft anomalies according to age and sex distribution, site distribution, characteristics of mass on physical examination, location of mass, preoperative diagnosis, type of branchial cleft cyst and report our experience with 25 cases of branchial cleft anomalies. Materials and Methods : A review of medical records was performed on 25 cases of branchial cleft anomalies diagnosed in pathologic report from January, 1990 to March, 2000. Result : Sex distribution was 11 male and 14 female, age distribution was most common in third decade which was 8 cases(32%). Site distribution was predominant in left side(13 cases), and in physical examination, the characteristics of the mass was nontender(76%), mobile(60%), soft(80%). The lesion site was submandibular area in 2 cases, upper 1/3 portion of sternocleidomastoid muscle(SCM) in 2 cases, middle 1/3 portion of SCM in 20 cases and parotid area in 1 case. The preoperative diagnosis was branchial cleft cyst in 16 cases, cervical lymphadenitis in 3 cases, tuberculous lymphadenitis in 3 cases, branchial cleft fistula in 1 case, dermoid cyst in 1 case and parotid mass in 1 case. Among 25 cases, first branchial cleft cyst was seen in 4 cases, second branchial cleft cyst in 20 cases and second branchial cleft fistula in 1 case. Conclusion : The clinical history of recurrent neck inflammatory episodes in young patients and nontender, mobile, soft neck mass should raise the suspicion of this entity. Investigation using CT scanning in combination with aspiration is useful. Treatment of all branchial cleft anomalies is complete surgical excision because there is lack of spontaneous regression, a high rate of recurrent infection, the possibility of other diagnosis, and rare malignant degeneration. Complete removal without complications depends on a good understanding of regional embryogenesis, a knowledge of the surrounding structure, and awareness of the different anatomical presentation.

광주광역시 전문계 고등학교 특성화 및 종합발전방안

나승일,정철영,김진모,안강현,한홍진,김기용,민상기,장현진 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.1

이 연구의 목적은 광주광역시 전문계 고등학교의 특성화 및 종합발전방안을 수립하는데 있다. 이 연구는 ① 전문계 고등학교 관련 선행연구 분석,② 인력육성 및 특성화 방향을 설정하기 위하여 광주광역시,전라남도,우리나라의 산업여건 분석,③ 광주광역시 전문계 고등학교에 대해 Malcolm Baldrige 평가모형에 의한 진단평가와 SWOT 및 활용전략 분석, ④ 광주광역시 전문계 고등학교의 개편 요구 분석,⑤ 광주광역시 전문계 고등학교의 특성화 및 종합발전방안 설정 등의 과정을 통해 수행되었다. 이 연구의 결과는 다음과 같다. 첫째,비전은 광주광역시 전문계고 특성화와 발전 역량 구축을 통한 산업체 요구에 부합하는 우수 인력 양성이며,이를 위한 전략은 특성화 체제구축,학교교육 혁신,인적 자원 혁신,학교경영 혁신,인프라 구축으로 설정하는 것이 필요하다. 둘째, 특성화 체제 구축은 특성화 체제 개편,교육과정 중심 특성화,학과 재배치 통합형 고교 도입,특성화고 신설을 통해 추진할 수 있 는데 특히,교육과정 중심 특성화가 적극 도입되어야 한다. 셋째,학교교육 혁신은 교육과정 개편,교수 학습 개선,진로지도 개선,시설 및 기자재 개선을 통해 추진할 수 있다. 넷째,인적자원 혁신은 학교 관리자 역량 강화,교원 역량 강화,학생 역량 강화를 통해 추진할 수 있다. 다섯째,학교경영 혁신은 경영 리더십 제고,경영 성과관리 강화,학교 재정 혁신을 통해 추진할 수 있다. 여섯째,인프라 구축을 위해 지자체,대학,산업체,지역주민과 연계 강화를 통해 추진할 수 있다. 일곱째,13개 전문계 고둥학교의 여건 및 특성에 적합한 단위학교별 특성화 및 종합발전방안을 수립하였다. The purpose of this study was to suggest strategies to specialize and develop vocational high schools in Gwangju Metropolitan City. The study was orderly performed as follows: (1) reviewing literature about vocational high school, (2) analyzing the condition of industries, (3) diagnosing the vocational high school through Malcolm Baldrige model, (4) analyzing the SWOT and application strategies, (5) analyzing the needs of reform, and (6) establishing the strategies for the specialization and development of Vocational High Schools in Gwangju Metropolitan City. The major findings of this study were as the following: First, the vision was to bring up excellent human resources to corresponds industrial needs through specialization and development of vocational high schools in Gwangju Metropolitan City. Five strategies to achieve it were creating the specialization system, innovating the school education, human resources, and the school management, and building the infrastructure. Second, creating the specialization system can be performed through five tasks which were restructuring the specialization system, creating curriculum-based specialization, trading departments in inter-schools, introducing an integrated high school, and building a new specialized high school. Especially curriculum based specialization needs to be accepted. Third, innovating the school education can be performed through four tasks which were reorganizing the curriculum, improving the teaching and learning, improving the career guidance, and improving the facilities and equipments. Fourth, innovating human resources can be performed through three tasks which were enhancing each competencies of a school manager, a teacher, and a student. Fifth, innovating the school management can be performed through three tasks which were improving the management leadership, developing the performance management, and innovating the school finance. Sixth, building the infrastructure can be performed through four tasks which were enhancing the relationships with a local government, colleges, industries, and local residents. Seventh, individual strategies for the specialization and development of thirteen vocational high schools were also developed by corresponding to their conditions and characteristics.