자궁 경부의 상피내암, 침윤이 있는 상피내암과 침윤성 편평상피암에서 p53, Ki-67 및 HER-2/neu의 표출 양상

장우섭,오미혜,양승하,김의한 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

To observe the expression of p53, Ki-67 and HER-2/neu in uterine cervical lesions, such as 10 cases of carcinoma in situ(CIS), 7 cases of carcinoma in situ with stromal invasion and 8 cases of invasive squamous cell carcinoma without carcinoma in situ, and to evaluate the corrleationship between results, the author has been carried out immunohistochemical staining with monoclonal antibodies to p53, Ki-67 and HER-2/neu. So, following results are obtained. 1. The p53 show negative reaction in all cases of CIS, and similar patterns in CIS with stromal invasion and invasive squamous cell carcinoma. 2. Ki-67 show high proliferation pattern in all cases and the basal cell show proliferating activity. 3. HER-2/neu show higher score in area of CIS than invasive area in stroma. 4. The stain pattern of HE-2/neu and p53 appeared to be inverse proportion to each other

비면역성 태아수종

장우섭 ( Woo Sup Chang ),최재호 ( Jae Ho Choi ),신봉식 ( Bong Sik Shin ),김범영 ( Bum Young Kim ),이교원 ( Kyo Won Lee ),송혜섭 ( Hye Sup Song ),한종설 ( Jong Seol Han ),김성도 ( Sung Do Kim ) 대한주산의학회 1998 Perinatology Vol.9 No.1

포스타 발표 : 산과학 ; 임신 14주에 초음파 검사로 진단된 멕켈-그루버 증후군 1예

김수진,안은희,장성운,장우섭,정상희,신승주,문명진 대한산부인과학회 2004 대한산부인과학회 학술대회 Vol.90 No.-

SF-36을 이용한 자궁적출술을 시행한 여성의 건강상태 평가

장경호,김봄님,김세현,심성신,장우섭,장성운,김상우 대한한방부인과학회 2004 大韓韓方婦人科學會誌 Vol.17 No.4

Purpose : The purpose of this study was to evaluate health status in women who undertaken a surgery for hysteretomy. Method : The informations taken from 60 patients who visited the department of ObGy in Bundang CHA Hospital from January, 2004 to November, 2004. We divided into two groups, experimental(N=30) and control(N=30). The members in the experimental group undertaken hysterectomy, a control group who did nor undertaken hysterectomy. The general data were collected that were age, height, weight, past history etc. Health status was measured using by SF-36(Medical Outcome Study Short Form 36), a 36 item self administered instrument. Results : The finding as follow; We compared to normartative data, patients exhibite impairment in all eight domains. The control group reported good health on the Physical functioning, Social Funtioning, Mental Health, Bodily Pain, General Health than experimental group. Conclusion : The results of this study suggest that carefulness is demanded to preform the hysterectomy, and positive managements to treat the symptoms after hysterectomy was demanded. Our results support proper management plan to improve the quality of lives in women who undertaken hysterectomy.

貼帶療法이 分娩에 미치는 영향에 관한 연구

김상우,장경호,김봄님,김세현,이동규,장성운,신승수,장우섭,강석호 대한한방부인과학회 2004 大韓韓方婦人科學會誌 Vol.17 No.2

Purpose : The aim of this study is to investigate the effects of taping therapy from the view of the primipara's having easy laboring(Sunsan). In this study, both total time and mode of labor were observed. Method : The 58 primipara who have visited to deliver on due in department of ObGy in Bundang CHA Hospital from October, 2002 to September, 2003 divided to two groups, experimental(N=30) and control(N=28). The members in the experimental group have taken the (subcutaneous) taping therapy on Acupoints in the accelation phrase in labor process I. The members in the control group was selected by random sampling within the same period. Then we estimated total necessary time from the activation phase to the full stage and followed up the pattern of labor, NSVD and C-sec. The general data were collected that were age, height, weight, past history etc, and the data reflecting the rate of the labor that Bishop score, NST, Apagar score, breakage of the amnion in labor. Results : The duration of labor of the experimental group was 227.2 33.1(min) and that was 240 35.7(min) in control group, then there was no significant difference in two groups, irrespective of the slight reduction of time. In respect of mode of delivery, there were 19 childbirth on NSVD(Sunsan) of 30 in the experimental group comparing to the 16 childbirth on C-sec(Nansan) of 28 in the control group. Also there was no significant difference in two groups. Conclusion : According to the above result, the taping therapy was not effective more than control group to help primipara to give easy birth.

埋鍼療法이 分娩에 미치는 영향에 관한 연구

장경호,송화숙,김세현,이동규,장성운,신승수,장우섭,강석호 대한한방부인과학회 2003 大韓韓方婦人科學會誌 Vol.16 No.2

자간전증 산모에서 증가된 혈장 호모시스테인의 위험인자로서의 임상적 유용성 연구

김성도,한종설,송혜섭,이교원,김범영,최익준,신봉식,장우섭,조현구 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.10

목적: 자간전증이 있는 미산부에서 혈중 호모시스테인 농도가 증가하는지를 조사하여 혈중 호모시스테인이 자간전증에서 위험인자로서의 임상적 유용성이 있는가에 대하여 연구하였다. 연구 방법: 1997년 10월부터 1998년 4월까지 강북 삼성병원 산부인과에 분만을 위해 입원한 미산부중 자간전증이 있었던 미산부 20명(중증: 9명, 경증: 11명; 20명 중 13명: 조기발병 자간전증미산부)을 환자군으로 정하고 자간전증이 없었던 미산부 20명을 대조군으로 선정하여 이들 40명 모두의 혈액을 채취한 다음 high performance liquid chromatography로 혈장 총호모시스테인 농도를 측정하였고, 방사선면역측정법으로 엽산과 비타민 B12 농도를 측정하였다. 결과: 자간전증이 있었던 미산부에서 호모시스테인 농도는 12.61±3.06 μmol/L로서 자간전증이 없었던 정상 미산부에서의 6.63±1.72 μmol/L보다 통계적으로 유의하게 높았고(p<0.05), 조기에 자간전증이 발병했던 13명의 미산부에서 호모시스테인 농도는 12.71±3.41 μmol/L로서 자간전증이 없었던 정상 미산부에서의 6.63±1.72 μmol/L보다 통계적으로 유의하게 높았다(p<0.05). 엽산의 경우 환자군에서 6.86±2.96 nmol/L로서 대조군에서의 8.17±3.26 nmol/L와 통계적으로 유의한 차이가 없었으며, 비타민 B12 경우에도 환자군에서 237.8±73.1 pmol/L로서 대조군의 277.4±68.9 pmol/L와 통계적으로 유의한 차이가 없었다. 환자군에서 혈중 호모시스테인 농도가 중증군에서 13.79±3.23 μmol/L, 경증군에서 11.65±2.68 μmol/L로 두 군간에 통계적으로 유의한 차이가 없었다. 결론: 자간전증이 있는 미산부에서 분만당시에 측정한 혈중 호모시스테인은 증가하며, 자간전증을 예측할 수 있는 위험인자로 유용될 수 있을 것으로 사료된다. 호모시스테인이 자간전증 발병에 어떤 역할을 하는지, 그리고 자간전증을 어떻게 예방할 수 있는지에 대한 더 많은 연구가 필요할 것으로 사료된다. Objective: This study was designed to determine whether homocyst(e)ine is elevated in nulliparous pregnant women with preeclampsia, and to evaluate a clinical usefulness of elevated plasma homocyst(e)ine with preeclampsia as a risk factor. Methods: Of 40 nulliparous pregnant women studied from October, 1997 to March, 1998, 20 had preeclampsia (severe: 9, mild: 11; 13 early onset preeclampsia of 20) and 20 were normal pregnant controls. We measured plasma homocyst(e)ine levels by high performance liquid chromatography , and folic acid and vitamin B12 concentrations by radioimmunoassay at the time of their delivery. Results: Mean (standard deviation) plasma homocyst(e)ine levels in the two groups, 20 nulliparous women with preeclampsia and 13 nulliparous women with early onset preeclampsia, were significantly higher than in the 20 nulliparous women without preeclampsia, respectively (12.61±3.06 versus 6.63±1.72 μmol/L, P<0.05; 12.71±3.41 versus 6.63±1.72 μmol/L, P<0.05). Folic acid and vitamin B12 concentrations were not significantly different between two groups, respectively (folic acid, 6.86±2.96 versus 8.17±3.26 nmol/L; vitamin B12, 237.8±73.1 versus 277.4±68.9 pmol/L). Mean (standard deviation) plasma homocyst(e)ine levels were not significantly different between severe and mild preeclampsia(13.79±3.23 versus 11.65±2.68 μmol/L). Conclusion: Homocyst(e)ine levels are elevated in nulliparous pregnant women with preeclamsia at the time of their delivery and might be useful to predict the preeclampsia as a risk factor. Further studies are required to determine what role homocyst(e)ine may play in the etiology of preeclampsia, and to investigate how to prevent preeclampsia.

융모막 세포에서 다중중합효소 연쇄반응을 이용한 Duchenne형 근이영양증의 산전 진단

이교원(Kyo Won Lee),윤선웅(Seon Woong Yoon),장우섭(Woo Sup Chang),김진영(Jin Yeong Kim),이숙환(Sook Hwan Lee),이상준(Sang Joon Lee),김성도(Sung Do Kim),한종설(Jong Sul Han) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.9

목적: 엑손 결실로 인한 근 이영양증(DMD)의 가족력이 있는 경우 융모막 검사로써 다중중합효소연쇄반응을 이용한 산전진단의 효용성에 관한 연구이다. 연구방법: 융모막 샘플링 후 염색체 분석으로 태아 성별을 확인하고 10일정도 더 배양하여 바닥면적이 25cm2가 되는 T-flask에서 1×106 cells 정도일 때 DNA를 추출하였다. 평균 DNA의 농도는 50-100 ng정도였고 DNA 추출 후 다중중합효소연쇄반응을 시행하였다. 결과: 다중중합효소연쇄반응은 결실이 흔히 일어나기 쉬운 엑손 17개를 4그룹으로 나누어서 중합효소연쇄반응을 시행하였으며, 모두 17개의 엑손이 전부 다 증폭이 되어서 결실이 없는 것으로 판정을 내릴 수 있었다. 결론: 이 방법은 융모 세포의 DNA분석으로서 근 이영양증의 위험성이 높은 남성 태아의 산전진단 방법이 되며, 착상 전 진단이 불가능한 경우 임신 초기에 대신할 수 있는 유용한 방법이다. 즉, 엑손 결실 때문에 생긴 근 이영양증 환자가 있는 가족에서 산전진단으로 이용할 수 있다. Objective: To evaluate the efficacy of prenatal multiplex PCR with chorionic villi, in the case of family history of DMD due to exon deletion. Methods: DNA was extracted when cells' size are 1x 106 on T- flask surface area reach to 25 cm2 after 2 weeks from sex confirmation. Average DNA concentration was 50-100 ng and multiplex PCR test was performed from DNA extraction. Result: PCR was done for 17 exons devided into 4 groups. Seventeen exons were all amplified with their right size. Conclusions: This method is DNA analysis for prenatal diagnosis of DMD with chorionic villi in the family of DMD. This is useful when preimplantation genetic diagnosis is not available.

제왕절개술 시행 중 조기 예측되어진 폐혈전색전증 1례

김원중 ( Won Joong Kim ),신승주 ( Seung Ju Shin ),장우섭 ( Woo Seob Jang ),김병성 ( Byoung Sung Kim ),김광현 ( Kwang Hyun Kim ),최미영 ( Mi Young Choi ),박찬 ( Chan Park ) 대한주산의학회 2001 Perinatology Vol.12 No.3

진피내 모반 , 경제 모반과 악성 흑색종에서 p53 과 p21 의 발현

송무현(Mu Hyun Song),김영태(Young Tae Kim),장우섭(Woo Sub Jang),이성열(Sung Yul Lee),양승하(Seung Ha Yang),이종석(Jong Suk Lee),황규왕(Kyu Uang Whang),김의한(Eui Han Kim) 대한피부과학회 2002 대한피부과학회지 Vol.40 No.8

N/A Background : The function of the p53 protein is known to regulate cell proliferation by inhibiting cells entering S phase, so DNA damaged cell proliferation is inhibited by apoptosis. p21 is a cyclin dependent kinase inhibitor induced by wild type p53, not mutant p53. Thus p21 is thought to mediate the signal of p53 induced by DNA damaged agents to arrest the cell cycle in G1 phase. p53 and p21 are expressed in many malignant tumors, and its role in oncogenesis, tumor progression and prognosis are important. Objective : The purpose of this study was to analyze immunogistochemical expression of mutant p53 and p21 protein in melanocytic lesions. Method : 11 cases of intradermal nevus, 7 cases of junctional nevus and 6 cases of malignant melanoma were immunohistochemically stained with p53 and p21 monoclonal antibodies. Results : 1. In intradermal nevus, the p53 was negative in 100% and the p21 was negative in 98%. These findings suggest that the composing cells of intradermal nevus is completely mature cell. 2. The positive rates of p53 and p21 in junctional nevus were 43% and 43%, respectively. The positive rates of p53 and p21 in malignant melanoma were 82% and 67%, respectively. Conclusion : If the expression of p21 is induced by p53 independent pathway, the cell cycle can be arrested in G1 phase, so the tumor cell proliferation is inhibited. But if the expressed p21 is mutated as p53, it means that the natural function of p21 disappears. More research is necessary about the nature of p21 which is expressed with mutant p53.