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- 저자 : ( Fangyu Cheng ) (검색결과 2 건)
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Biosynthesis of Chondroitin in Engineered Corynebacterium glutamicum
( Fangyu Cheng ),( Sijin Luozhong ),( Huimin Yu ),( Zhigang Guo ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
Chondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum. CgkfoC and CgkfoA genes with host codon preference were synthesized and driven by promoter Ptac, which was confirmed as a strong promoter via GFPuv reporter assessment. In a lactate dehydrogenase (ldh) deficient host, intracellular chondroitin titer increased from 0.25 to 0.88 g/l compared with that in a wild-type host. Moreover, precursor enhancement via overexpressing precursor synthesizing gene ugdA further improved chondroitin titers to 1.09 g/l. Chondroitin production reached 1.91 g/l with the engineered strain C. glutamicum ΔL-CgCAU in a 5-L fed-batch fermentation with a single distribution M<sub>w</sub> of 186 kDa. This work provides an alternative, safe and novel means of producing chondroitin for industrial applications.
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Dou-dou Liu,Jian-fang Li,Xiaomao Li,Liangjun Xie,Luping Qin,Fangyu Peng,Mu-hua Cheng 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.6
Objective: To investigate the prognostic value of metabolic tumor volume (MTV) and totallesion glycolysis (TLG), measured by preoperative 18F-fluorodeoxyglucose positron emissiontomography/computed tomography (18F-FDG PET/CT), in risk stratification of patients withendometrial carcinoma (EC). Methods: The patients with pathological diagnosis of EC who underwent preoperative18F-FDG PET/CT imaging were retrospectively selected for analysis of the prognostic valuesof PET parameters in risk classification and lymph node metastases (LNMs). Receiveroperating-characteristic analysis was used to analyze the correlation of PET parameters cutoffvalues with deep myometrial invasion (MI), lymphovascular space involvement and LNM forprognostic values in risk stratification. Results: The sensitivity, specificity, positive predictive value, negative predictive value andaccuracy for detection of LNM are 83.3%, 99.7%, 90.9%, 99.5% and 99.2%, respectively. TheMTV and TLG of primary lesion of EC in the patients with LNM are notably higher than thosein patients without LNM, p<0.010. The MTV and TLG of the EC primary lesions in high-riskpatients are significantly higher than those in low-risk patients (p<0.010), but the maximumstandardized uptake value (SUVmax) is not. The MTV and TLG of primary lesions weresuperior to SUVmax for predicting of deep MI, LNM and high-risk of EC (p<0.005). Conclusion: MTV and TLG of primary lesions are more valuable in predicting riskstratification of EC patients. Preoperative 18F-FDG PET/CT imaging is useful in predictingthe LNM of EC and may help guide pelvic lymphadenectomy to avoid unnecessary pelviclymphadenectomy in EC patients with low-risk stratification.
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