진폭 비선형 양자화에 의한 컴퓨터 형성 홀로그램에 관한 연구

김은수,양인응 光云大學校 1987 論文集 Vol.16 No.-

In this paper, the binary computer generated hologram by amplitude nonlinear quantization method was analyzed. We observed that the intensity of the reconstructed image was increased with the nonlinear parameter μvalue & the image quality & light efficiency of the reconstructed image was found to be better those of linear quantization method.

데빅병 치료에 사용한 Interferon β-1b의 부작용 1예

김정미,조은경,조정선,최용석,한영수,한정호,김두응,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

A 63 year-old woman who had diagnosis of multiple sclerosis complaining left hemiparesis and fluctuating decreased visual acuity in her right eye and abnormal findings in pattern shift visual evoked potentials(PSVEPs) and on T2 weighted MR scanning. We treated her subcutaneously with 250μg interferon (3-lb(ITFß -lb) every other day. There were detected pancytopenia, skin rash, and abnormal liver function test seven days after the therapy. In spite of reducing the dose of interferon injection, abnormal laboratory findings were not returned to normal. Dose adjustment of interferon-I3 for body weight or race, may be helpful to reduce the side effects.

흔치않은 대상포진 치험 2예 : 비전형적 부위와 다른 부위로 재발한 예 Unusal Dermatome and Recurs Other Dermatome

김정미,조은경,조정선,최용석,한영수,한정호,김두응,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Herpes zoster is well known as an acute, self-limited disease of unilateral sensory dermatome caused by varicella-zoster virus and characterized by grouped vesicular skin eruption and pain over several dermatomes as well as single. This virus usually affects the thoracic dermatome and there are a few reports that this virus involves lower lumbar or sacral area, and recurrent cases. We experienced 2 cases that zoster virus involved L4 or S2 dermatome and left typical skin lesions, of which one was a recurrent case involving different dermatome at an interval of 8 years and the other was a rare case involving sacral dermatome.

미오신 II에 의한 신경 성장추의 액틴 다이내믹스 조절

신은영, 김응국 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적: 미오신 II는 액틴과 결합해서 세포의 다양한 활성, 수축, 이동, 부착 등을 조절한다. Rho G 단백질도 유사한 역할을 수행한다. 따라서 미오신 II와 Rho G 단백질의 활성 조절은 밀접하게 연 관되어 있다. 본 연구에서는 신경 성장추를 모델로 이 두 개의 단백질이 어떻게 액틴 다이내믹스를 조 절하는가에 관한 연구를 수행코자 한다. 대상 및 방법: 신경 성장추는 쥐의 해마세포를 초대배양하여 확보한다. 미오신 II에 대한 저해제인 blebbistatin (BBS)을 처리한 후 신경 성장추의 액틴 골격의 변화를 관찰한다. 또한 신경세포의 신경 돌기 및 성장추의 확장을 촉진하는 인자인 신경성장인자를 처리하고 미오신 II의 인산화를 면역블롯과 면역화학법을 통해 관찰한다. 결과: 초대배양한 해마세포의 신경 성장추에 BBS를 처리하면 30분 내에 질서정연한 액틴 골격이 무 질서한 액틴 골격으로 변한다. BBS를 배양액에서 제거하면 서서히 원래의 형태로 전환되는 것으로 미 루어, BBS에 의한 액틴 골격의 변화는 가역적인 반응으로 추정된다. 신경성장인자를 처리하면 BBS에 의해 유도되는 급격한 변화는 보이지 않았다. 그러나 미오신 II의 인산화를 면역블롯과 면역염색을 통 해 확인할 수 있었다. 결론: 미오신 II는 인산화를 통해 Rho G 단백질의 활성과 신경 성장추의 액틴을 재구성하는 것으로 보인다.

일단계 핵산 추출을 이용한 사람거대세포바이러스와 돼지내재레트로바이러스의 검출법

김정헌,정은숙,황응수 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Xenotransplantation is thought to be one of the alternative methods to overcome the shortage of human organs for transplantation. Recipients should be immunosuppressed for graft survival, and thus, there is a need for developing diagnostic modality that can detect diverse infections originating from animals and recipients rapidly, in the early stage, and with high sensitivity using small volume of samples. This study was carried out to develop a fast, simple, and robust technique for the preparation of HCMV DNA and PERV RNA using small volume of samples. Materials and Methods: Nucleic acids were extracted from serially diluted samples with one step extraction method as well as with Qiagen kit. The presence of genomic DNA of human cytomegalovirus (HCMV) and porcine endogenous retrovirus (PERV) was detected by PCR and specific primer set, respectively. RNA of HCMV and PERV was extracted and then detected by RT-PCR and specific primer set, respectively. For absolute quantification of HCMV, standard curve was established by real time PCR. Results: HCMV DNA and PERV RNA were prepared from culture supernatant and cells for PCR or RT-PCR with one step extraction method. It was possible to extract both the DNA and RNA from the samples in about 20 minutes with one step extraction method in a single tube. HCMV and PERV could also be detected by PCR and one step extraction method, respectively. It was also good with small quantity samples. Conclusions: One step extraction method is simpler and faster method than other extraction methods when there are two types of DNA and RNA viruses in one sample. From these results, we could see that the one step extraction method could be very useful in detecting HCMV and PERV rapidly from the pig cells or organ transplanted recipients with a small amount of sample.

정적 부하의 비대칭적 적용에 따른 등 근육의 근전도 분석

김태영,박은영,이응상 한국전문물리치료학회 1997 한국전문물리치료학회지 Vol.4 No.1

網膜色素上皮細胞의 Lysosomal acid phosphatase의 活性値 測定

김복희,김은경,김윤화,백미화,이지숙,조영숙,황응숙 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

Measurement of lysosomal enzyme activity in the rabbit eye was effected using acid phosphatase as enzyme markers. Acid phosphatase in the retinal pigment epithelium of the rabbit eye was studied using standard enzymological techniques. The enzyme activity in the lysosomal extract of the pigment epithelium was 54.9±23.0 King-Armstrong Unit/dl.

SNU 미니돼지 유래 돼지내재레트로바이러스의 시험관내 사람세포주에서의 증식부전

김정헌,정은숙,박정규,김상준,황응수 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: The presence of porcine endogenous retrovirus (PERV) has been considered as one of the main hurdles to transplant pig’s organs or tissues to human beings. There has been no report that PERV infection is associated with human diseases. Because pigs have their own characteristics of PERV according to pig strain, it is necessary to analyze the infectivity of PERV from SNU miniature pig to human cells for future utilization as a transplantation donor. Materials and Methods: Human cell lines were infected with culture supernatant from porcine cell line or immunomodulator-stimulated peripheral blood mononuclear cells (PBMC) of SNU miniature pigs. They were also co-cultured with PBMC or islet cells of SNU miniature pigs. The presence of PERV genes and general pig marker gene in cells was determined by nested PCR with primer set for PERV pol and pig mitochondrial cytochrome oxidase II (COII), respectively. Results: Infection test with the culture supernatant from PBMC of SNU miniature pigs showed that PERV pol but not COII was detected only in a few cases, but there was no uniform infection pattern in scope of stimulators and cell types. PERV pol was not demonstrated in co-cultures of human cell line with PBMC or islet cells from SNU miniature pigs after 80 days of co-cultures. Conclusions: In vitro infectivity test suggests that PERV from SNU miniature pig might not replicate productively in human cell lines although it could infect human cells and integrate into chromosome.

Rho GTPase와 미오신 활성의 상관관계 조사

김응국, 신은영 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적 : 본 연구는 PC12 세포에서 nerve growth factor (NGF) 처리에 의해 Rho GTPase Rac이 활성화 되었을 때 미오신 II와 guanine nucleotide exchange factor (GEF) PIX의 상호작용을 관찰하고자 하였다. 재료 및 방법 : PC12 세포는 10% 혈청이 포함된 DMEM에서 배양하였다. NGF는 처리 전 24 시간 동안 혈청을 제거하였다. NGF 20 ng/ml를 표기한 시간대로 처리한 후 단백질 용출액을 처리하여 세포균질액을 얻었다. 이 과정을 통해 얻은 세포균질액은 PBD-His beads로 pulldown assay를 실시하여 Rac의 활성화를 검증하였다. 또한 anti-PIX antibody로 면역침전한 후 미오신 II 항체를 이용하여 결합여부를 확인하였다. 결과 : Rac의 활성은 NGF 처리 후 5분부터 20분까지 꾸준히 증가하였으며 30분에는 감소 하는 경향을 나타냈다. 뿐만 아니라 PIX와 미오신 II와의 결합은 10분부터 감소하기 시작하 여 20-30분에는 결합한 두 단백질의 양이 상당히 감소함을 확인할 수 있었다. 결론 : 이상의 결과로부터 NGF 처리에 의해 Rac이 활성화 되는 것과 PIX와 미오신 II의 결합이 해리되는 시간대가 일치한다는 것을 알 수 있었다. 이는 NGF 처리가 PIX와 미오신 II의 결합 상태를 해리시켜 PIX의 GEF 활성을 유도한다는 것을 설명하고 있다.

치오포스포티로신을 함유한 펩티드 유도체의 중간체 합성

김은경,최희성,이응석 영남대학교 약품개발연구소 1998 영남대학교 약품개발연구소 연구업적집 Vol.8 No.-

N-(tert-Butoxycarbonyl)-O-(dicyanoethylthiophosphono)-L-tyrosine(7), the key intermediate for the synthesis of thiophosphotyrosine-containing peptide derivatives, was prepared. For the phosphorylation, we used t-Boc-tyrosine and phosphoramidite in the presence of 1H-tetrazol. For the protection of thiophosphate moiety, cyanoethyl protecting group was used. Thiophosphotyrosine-containing peptides could be used as tools fur the elucidation of mechanism of signal transduction pathway and also prepared as PTK inhibitors. PTPase inhibitors and cytosolic protein binding blockers. It may be contributed for the development of potential anticancer agents.