赤血球의 Methylene Blue攝取 와 Pentose 形成能에 미치는 高溫保管의 影響

池垠政 全北大學校 1975 論文集 Vol.17 No.-

Human erythrocytes were incubated at 30°for various time intervals up to 7 days in Na-phosphate buffer(pH 7.4) or acid-citrate-dextrose(ACD) solution. During incubation, the uptake of methylene blue and the formation of pentose from glucose or inosine were assayed to observe the effect of storage at high temperature(30°) on the glucose metabolism of erythrocytes. During incubation of red cells at 30°in Na-phosphate buffer, the methylene blue uptake in the presence of glucose decreased rapidly, and the formation of pentose from glucose also decreased gradually. The concentration of pentose in the erythrocytes was not correlated to the activity in converting pentose to glucose-6-phosphate. In contrast when inosine was added in place of glucose to red cell suspension, the methylene blue uptake and pentose formation remained unchanged during the incubation, and also the activity of converting pentose to glucose-6-phosphate was well correlated to the pentose concentration in the cells. The erythrocytes incubated at 30°in ACD solution showed the similar changes in methylene blue uptake, penotse formation in the presence of glucose, and the converting activity of pentose to glucose-6-phosphate, however, when inosine was substrate, methylene blue uptake, pentose formation, and the converting activity of pentose to glucose-6-phosphate decreased gradually during incubation. These results suggest that the incubation of erythrocytes at 30°causes the rapid depletion of adenosine triphosphate(ATP) in the erythrocytes, leading to the decreased activity in nucleoside phosphorylase and in the conversion of triose phosphate to glucose-6-phosphate in Embden-Meyerhof Pathway. It is also suggested that the activity in the formation of triose phosphate from pentose in pentose phosphate pathway and in the later half of Embden-Meyerhof Pathway (triose phosphate→lactate) is relatively well preserved during the incubation of red cells at 30°up to 7 days.

Inhibition of Aflatoxin Indrced Hepatocarcinogenesis by Antioxidant, BHA

지은정 啓明大學校 醫科大學 1989 계명의대학술지 Vol.8 No.2

Aflatoxin which is widely distributed in the human food supply is extremely potent animal toxins and is welknown hepatocarcinogen. Primary liver cancer is not prevalent form of cancer in western countries, however, there are certain geographical area where the incidence is significantly elavated, namely southeast Asia, China, southeastern India and subsaharan Africa, where temperature and humidity would favor contramination of human food stufls.

닭赤血球膜 Ca^(2+)-Adenosine Triphosphatase에 關한 硏究

金炯魯,池垠政 全南大醫大 1975 전남의대학술지 Vol.12 No.2

열안정형 카테콜-O-메틸전이효소 유전자의 클로닝

홍경만,최용복,정갑용,지은정,장현신,박현,백문기 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Catechol-O-methyltransferase (COMT; EC 2.1.1.6) is the enzyme which catalyzes the transfer of methyl group to the catecholamine neurotransmitters from its methyl donor S-adenosyl-L-methionine. Partially purified COMT from rat liver has been used to measure the concentration of catecholamines in the blood through solvent extraction and thin layer chromatography after converting ^3H-methyl derivatives of catecholamines. To improve this inconvenient and fluctuating method, an attempt was made to use COMT gene for the measurement of catecholamines. Specific primers, COMT5P (5'-TGC TCA GAG GTG CTT TGA AG-3') and COMT3P (5'-GGA GCC GCA GAA GGT CA G-3'), were used to amplify COMT gene from human placenta cDNA library. The amplified COMT gene through 35 cycles of polymerase chain reaction was cloned into T-vector and the nucleotide sequences are determined by automatic sequencer. Human COMT has two common variants, a thermostable high activity form which has valine at amino acid 158 and a thermolabile low activity form which Has methionine at the same position. The cloned COMT gene in this study has both variants. Clone 4 and 5 have valine at amino acid 158 (guanine at nucleotide 472) and clone 1-3 have methionine at this site (adenine at nucleotide 472). In addition to this, there are other DNA polymorphisms in COMT gene at nucleotide 101, 102 (amino acid 34, a structural mutation cysteine/serine) and nucleotide 186 (a silent mutation). All of the clones have cysteine at amino acid 34 (guanine and adenine at nucleotides 101 and 102) and cytosine at nucleotide 186 except clone 3 which has thymine at this nucleotide. Clone 4 and 5 are thermostable high activity variants, suggesting being more useful for the measurement of catecholamines after expressing the gene.

골수암유래 암세포주 KHOS/NP와 MG-63의 성장에 미치는 마늘의 암세포 독성효과

이정석,임성남,오명숙,지은정 全北大學校 齒醫學硏究所 1993 전북치대논문집 Vol.11 No.1

Most anticancer drugs so far used have brought many side effects to the host, which is inevitable since they can not specifically distinguish between cancer cells and rapidly dividing normal cells. Therefore, it is very important to develope a chemopreventive agent proven for a long period of time not to toxic to normal cells. Since garlic has been attracted interests from many areas of cancer researchers as a potent anticancer agent, attempts were made to see the direct cytotoxic effect of garlic to human osteosarcoma cell lines. The present study was designed to investigate the effect of garlic on the two cell lines of rapidly growing osteosarcoma with comparable doubling time of 20 hours, namely KHOS/NP and MG-63. Four different garlic preparations commnly used in the Koreans, that is, raw and heated garlic dissolved in distilled water, raw and heated garlic dissolved in saline were compared in thier cytotoxic effect to the KHOS/NP and MG-63. The results obtained were as follows. 1. Raw garlic dissoved in distilled water showed the best cytotoxic effect among four different preparations of garlic used in this study. 2. The cytotoxic effect of garlic to the osteosarcoma was different, to the cell lines showing mostly more effective to KHOS/NP than to MG-63. 3. To KHOS/NP, the cytotoxic effect of garlic was markedly increased with concentrations but not with incubated time. 4. To MG-63, the cytotoxic effect of garlic w3s increased both with concentrations and incubated time. 5. The difference of inhibitory action of garlic between KHOS/NP and MG-63 was marked when heated garlic in saline was treated at high concentration(0.1875g% ) for 48 hours.

A flatoxin B_1 carcinogenesis and its prevention by Butylated hydroxyanisole

Jhee, Eun-Chung 全北大學校 齒醫學硏究所 1985 전북치대논문집 Vol.3 No.1

흰쥐에 butylated hydroxyanisole (BHA)를 전처치 한후 강력한 간암 유발제인 aflatoxin과 흰쥐간의 DNA와의 결합능의 정도를 생체내 실험은 물론 시험관내 실험에서도 비교관찰하였다. 대조군과 BHA로 전처치한 흰주의 간을 대상으로 aflatoxin-B_1 DNA 결합능을 비교해보면 cytochrome P450 함량이나 microsome에 연관된 aflatoxin B_1-DNA양에는 별차이가 없었다. cytosol의 저해효과는 대조군의 2배에 비하여 BHA전처치때는 5배나 저해 시키므로서 현저하게 저해 작용이 증진 됨을 알수 있었다. 이와같은 aflatoxin B_1-DNA결합능에 미치는 cytosol의 저해작용은 송아지 흉선의 exogenous DNA와 신선한 흰쥐의 간세포에서 직접 분리한 endogenous DNA에서 모두 비슷한 양상을 나타냈다. 1mM 농도의 trichloropropene oxide나 styrene oxide는 aflatoxin B_1-DNA결합능에 대한 cytosol의 저해 작용을 부분적으로 다시 환원 시킬뿐아니라 흰쥐를 희생시키기 2시간 전에 aflatoxin B_1을 복강내로 주사 한후 DNA와의 결합능을 관찰해 보면 대조군에서 보다 BHA로 전처치한 실험군에서 50%나 AF B_1-DNA 결합능이 감소되는 것을 볼 수 있었다. 이상의 결과로 미루어 볼 때 BHA로 전처치한 흰쥐에서는 GSHS-transferase가 유되되어 간의 Aflatoxin B_1-DNA 결합능을 조절하는 것이 시사되었다.

The Effect of Butylated Hydroxyanisole on Hepatic Afatoxin B₁-DNA Binding in Rats

Eun-Chung Jhee 한국식품과학회 1985 식품과학과 산업 Vol.18 No.3

Inhibition of Microsome-mediated Binding of Aflatoxin B1 to DNA by Glutathione S-transferase

Jhee, Eun Chung,Prabhaker D. Lotlikar 전북대학교 의과학연구소 1981 全北醫大論文集 Vol.5 No.1

The Properties of Ca^2+-Adenosine Triphosphatase in Chicken Erythrocytes Membrane

Chung, Jhee Eun 전북대학교 의과학연구소 1978 全北醫大論文集 Vol.2 No.-

Biotechnology of Aflatoxins

Jhee, Eun-Chung INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1989 YONSEI REPORTS ON TROPICAL MEDICINE Vol.20 No.1

Aflatoxins which is produced by the fungus, asperfillus flavus was widely distributed in the human food supply like cones, peanuts and grains. It has been wellknown to be a potent toxin causing acute aflatoxicosis liver cancer. As informations on aflatoxin problems accumulated, it has become apparent that the risk of exposure to aflatoxins is much less in technologically developed countries than in developing ones. The role of the hepattitis B virus and aflatoxin B₁in the developmetn of primary hepatocellular carcinoma (PHC) was studied in southeast Asia and Africa. Using aflatoxin B₁binding to DAN as an indicator of hepatocarcinogenic potency, the effect of antioxidant was examined to see how this agent modulate the hepatocarcino-genesis of aflatoxins in rats. We also find the mechanism of this inhibition by antioxidant is through the modulation of cytosolic detoxifying enzyme, glutathione s-transferases.