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Liu, Hui,Wang, Sixin,Zhang, Dongyan,Wang, Jing,Zhang, Wei,Wang, Yamin,Ji, Haifeng Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1
Objective: This study was conducted to determine the effects of dietary supplementation with Pediococcus acidilactici (P. acidilactici) ZPA017 as a probiotic on reproductive performance, fecal microbial flora and serum indices in sows during late gestation and lactation. Methods: A total of 94 sows (Large White×Yorkshire, average 4.50 parities) were randomly allotted to two dietary treatments: control diet and the diet supplemented with P. acidilactici ZPA017 (2.40×10<sup>9</sup> colony-forming unit/kg of diets). The study started at d 90 of gestation and conducted until d 28 of lactation. Results: Compared to sows fed the control diet, supplementation of P. acidilactici ZPA017 increased the number of weaning piglets, weaning weight of litter and piglets, survival rate of piglets at weaning (p<0.05), and decreased diarrhea rate of piglets in lactation (p<0.05). Dietary P. acidilactici ZPA017 increased fecal Lactobacillus populations (p = 0.030) and reduced fecal Escherichia coli and Staphylococcus aureus populations (p<0.05) of sows at weaning. Moreover, the supplementation of P. acidilactici ZPA017 increased serum concentrations of immunoglobulin G, immunoglobulin A and total protein (p<0.05), while decreased serum haptoglobin concentration and alanine aminotransferase activity (p<0.05) of sows at weaning. Conclusion: Administration of P. acidilactici ZPA017 in diets during late gestation and lactation had positive effects on the reproductive performance, intestinal microflora balance and immunity of sows.
Tianqi Liu,Dongyan Chen,Jun Hu 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.5
In this paper, the resilient distributed filtering problem is addressed for discrete stochastic uncertain timevarying systems with missing measurements and stochastic uncertainties over wireless sensor networks. Some random variables governed by the Bernoulli distribution are used to model the missing measurements phenomenon of each sensor node. In addition, the stochastic uncertainties are characterized by the multiplicative noises and stochastic nonlinearities. By using the variance-constrained method, an appropriate filter gain is selected to minimize thetrace of the upper bound for the filter error covariance. Moreover, the resilient distributed filtering algorithm isdesigned and a new matrix simplification technique is introduced to deal with the sparsity of sensor networks topology. Finally, both the feasibility and effectiveness of the resilient distributed filtering algorithm are verified by anumerical simulation.
Production and Optimization of a Kiwi Pectin Methylesterase Inhibitor in Pichia pastoris GS115
Qian Liu,Wentao Xu,Shiwen Han,Dongyan Cao,Xiaoyun He,Kunlun Huang,Xiaohong Mei 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6
Gene sequence coding of a kiwi pectin methylesteraseinhibitor was optimized, synthesized, and expressedin Pichia pastoris GS115 based on P. pastoris preferredcodon usage. The expression level of the recombinantprotein (kwPMEI) increased by 89.74% after codonoptimization. Expression conditions of recombinant strainswere optimized. The highest production of kwPMEI wasachieved using 0.8% sorbitol (added every 24 h), 0.05%oleic acid (added at the beginning of induction), and 0.5%methanol (added every 12 h). kwPMEI was purified usingNi2+ chelating affinity chromatography and 17 mg of theprotein was harvested from 60 mL of a culture supernatant. Activity analysis showed that kwPMEI efficiently inhibitedthe activity of different plant PMEs. High expression levelsand purification of kwPMEI will promote applications infruit and vegetable juices.
Linyan Zhou,Dongyan Liu,Ying Xie,Xinjie Yao,Yan Li 거트앤리버 소화기연관학회협의회 2019 Gut and Liver Vol.13 No.4
Background/Aims: The current study aims to investigate the protective effects of Bifidobacterium infantis on the abnormal immune response to inflammatory bowel disease (IBD) in dextran sodium sulfate (DSS)-induced colitis. Methods: Eight-week-old BALB/c mice were separated into five groups at random (control, DSS, DSS+B9 [B. infantis 1×109 CFU], DSS+B8 [B. infantis 1×108 CFU], and DSS+B7 [B. infantis 1×107 CFU]). Colitis was induced by 5% DSS ad libitum for 7 days, at which time we assessed weight, the disease activity index (DAI) score, and the histological damage score. The nuclear transcription factor Foxp3 (a marker of Treg cells), cytokines interleukin-10 (IL-10) and transforming growth factor β1 (TGF-β1), and related proteins (programmed cell death ligand 1 [PD-L1] and programmed cell death 1 [PD-1]) were detected by an immunohistochemical method and Western blot. Results: B. infantis increased weight, decreased DAI scores and histological damage scores, increased the protein expression of Foxp3 (p<0.05) and cytokines IL-10 and TGF-β1 in mouse colon tissue (p<0.05), and increased the expression of PD-L1 in the treatment groups relative to that in the DSS group (p<0.05). The effect of B. infantis on Foxp3 and PD-L1 was dose dependent in the treatment groups (p<0.05). PD-L1 was positively correlated with Foxp3, IL- 10, and TGF-β1. Conclusions: In a mouse model of IBD, B. infantis can alleviate intestinal epithelial injury and maintain intestinal immune tolerance and thus may have potential therapeutic value for the treatment of immune damage in IBD.
Topology-Hiding Broadcast Based on NTRUEncrypt
( Bo Mi ),( Dongyan Liu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.1
Secure multi-party computation (MPC) has been a research focus of cryptography in resent studies. However, hiding the topology of the network in secure computation is a rather novel goal. Inspired by a seminal paper [1], we proposed a topology-hiding broadcast protocol based on NTRUEncrypt and secret sharing. The topology is concealed as long as any part of the network is corrupted. And we also illustrated the merits of our protocol by performance and security analysis.
Qianying Zhao,Jiaxin Yang,Dongyan Cao,Jiangna Han,Kaifeng Xu,Yongjian Liu,Keng Shen 대한부인종양학회 2016 Journal of Gynecologic Oncology Vol.27 No.3
Objective: To explore the appropriate treatment of malignant germ cell tumor (MGCT) in thefemale genital system, and to analyze the factors influencing both therapeutic response andsurvival outcome. Methods: A cohort of 230-Chinese women diagnosed with MGCT of the genital systemwas retrospectively reviewed and prospectively followed. The demographic and pathologicalfeatures, extent of disease and surgery, treatment efficiency, recurrence and survival wereanalyzed. Results: MGCTs from different genital origins shared a similar therapeutic strategy andresponse, except that all eight vaginal cases were infantile yolk sac tumors. The patients’ curerate following the initial treatment, 5-year overall survival and disease-free survival (DFS)were 85.02%, 95.00%, and 86.00%, respectively. Although more extensive excision couldenhance the remission rate; it did not improve the patients’ survival. Instead, the level of themedical institution, extent of surgery and disease were independent prognostic factors forrelapse (p<0.05). Approximately 20% of patients had recurrent or refractory disease, morethan half of whom were in remission following secondary cytoreductive surgery with salvagechemotherapy. Conclusion: Fertility-sparing surgery with or without standardized PEB/PVB (cisplatin,etoposide/vincristine, and bleomycin) chemotherapy is applicable for female MGCTsof different origins. Comprehensive staging is not required; nor is excessive debulkingsuggested. Appropriate cytoreduction by surgery and antineoplastic medicine at anexperienced medical institution can bring about an excellent prognosis for these patients.
Stable pH sensitivity of LaAlO3/SrTiO3 interfacial electronic gas
Dong Yan,Zhang Lunyong,Li Chen,Liu Yanli,Shao Pengfei,Lei Jianming,Wang Rui,Wu Di,Chen Dunjun,Zhang Rong,Zheng Youdou 한국물리학회 2022 Current Applied Physics Vol.34 No.-
Probing pH value is essential for many applications. The request of developing stable and highly sensitive pH sensor with small size are increased recently, this is a challenge to the traditional glass pH sensors. This work investigated the pH sensing performance of the novel two-dimensional electronic gas (2DEG) at the LaAlO3/ SrTiO3 heterostructure interface discovered recently. The experimental results demonstrated that the devices host excellent sensing ability to the pH value of aqueous solutions. Quite stable output current is realized for a given pH value, and the output current is linearly dependent on pH value as required by sensor applications. This work would prompt the application research of LaAlO3/SrTiO3 heterostructure and design of new generation of advanced pH sensors.